--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/iwtomics-1.0/testandplot.R	Fri Oct 06 11:03:56 2017 -0400
@@ -0,0 +1,99 @@
+if (require("IWTomics",character.only = TRUE,quietly = FALSE)) {
+  args=commandArgs(TRUE)
+  
+  # get args names and values
+  args_values=strsplit(args,'=')
+  args_names=unlist(lapply(args_values,function(arg) arg[1]))
+  names(args_values)=args_names
+  args_values=lapply(args_values,function(arg) arg[2])
+  # read filenames
+  adjustedpvaluematrix=args_values$adjustedpvaluematrix
+  iwtomicsrespdf=args_values$iwtomicsrespdf
+  iwtomicssumpdf=args_values$iwtomicssumpdf
+  regionids=args_values$regionids
+  featureids=args_values$featureids
+  rdatafile=args_values$rdatafile
+  iwtomicsrdata=args_values$iwtomicsrdata
+  iwtomicstests=args_values$iwtomicstests
+  iwtomicsselectedfeatures=args_values$iwtomicsselectedfeatures
+  # read parameters (from region1 on)
+  i_region1=which(args_names=='region1')
+  for(i in i_region1:length(args)){
+    eval(parse(text=args[[i]]))
+  }
+  
+  # load RData
+  load(rdatafile)
+  # read regionids and featureids
+  regionids=as.character(read.delim(regionids,header=FALSE,sep='\t',stringsAsFactors=FALSE))
+  featureids=as.character(read.delim(featureids,header=FALSE,sep='\t',stringsAsFactors=FALSE))
+  # retrieve region1, region2 and features_subset ids and check they are in the RData
+  id_region1=regionids[region1]
+  id_region2=regionids[region2]
+  id_features_subset=featureids[features_subset]
+  if(length(setdiff(c(id_region1,id_region2),idRegions(regionsFeatures)))!=0){
+    write("Wrong region ids.", stderr())
+    quit(save="no", status=10)
+  }
+  if(length(setdiff(id_features_subset,idFeatures(regionsFeatures)))!=0){
+    write("Wrong feature ids.", stderr())
+    quit(save="no", status=20)
+  }
+  if(sum(duplicated(paste0(id_region1,id_region2)))){
+    write("Same test repeated multiple times.", stderr())
+    quit(save="no", status=30)
+  }
+  
+  # perform test
+  tryCatch({
+    # fix repeated probs
+    if(statistics=='quantile'){
+      # fix repeated probs
+      testprobs=sort(unique(testprobs))
+    }else{
+      testprobs=0.5
+    }
+    regionsFeatures_test=IWTomicsTest(regionsFeatures,id_region1,id_region2,id_features_subset,
+                                      statistics=statistics,probs=testprobs,B=B)
+    # create adjustedvaluematrix output
+    for(test in seq_along(id_region1)){
+      for(id_feature in id_features_subset){
+        write(paste0('Test: ',id_region1[test],' vs ',id_region2[test],', on feature ',id_feature),
+              file=adjustedpvaluematrix,append=TRUE,sep='\t')
+        pval=regionsFeatures_test@test$result[[test]][[id_feature]]$adjusted_pval_matrix
+        row.names(pval)=paste('Scale',rev(seq_len(nrow(pval))))
+        write.table(pval,file=adjustedpvaluematrix,append=TRUE,sep='\t',quote=FALSE,row.names=TRUE,col.names=FALSE)
+        write('',file=adjustedpvaluematrix,append=TRUE,sep='\t')
+      }
+    }
+  }, error = function(err) {
+    write("Testing error.", stderr())
+    quit(save="no", status=40) #error testing
+  })
+  
+  # plot test results
+  pdf(iwtomicsrespdf,width=5,height=7)
+  if(plottype=='boxplot'){
+    # fix repeated probs
+    probs=sort(unique(probs))
+  }else{
+    probs=c(0.25,0.5,0.75)
+  }
+  plotTest(regionsFeatures_test,alpha=testalpha,type=plottype,probs=probs,average=average,size=size,ask=FALSE)
+  dev.off()
+  
+  # plot summary results
+  if(groupby!='none'){
+    pdf(iwtomicssumpdf,width=15,height=10)
+    plotSummary(regionsFeatures_test,alpha=summaryalpha,only_significant=only_significant,groupby=groupby,ask=FALSE,append=TRUE)
+    dev.off()
+  }
+  
+  # create output
+  write.table(as.data.frame(t(paste(id_region1,'vs',id_region2))),file=iwtomicstests,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE)
+  write.table(as.data.frame(t(idFeatures(regionsFeatures_test))),file=iwtomicsselectedfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE)
+  save(regionsFeatures_test,file=iwtomicsrdata)
+}else{
+  write("Missing IWTomics package. Please be sure to have it installed before using this tool.", stderr())
+  quit(save="no", status=255)
+}
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