# HG changeset patch # User fabio # Date 1496243242 14400 # Node ID 565118df598a1cbcde57b76702e66727aaa18d1b # Parent 81faa1567178a2f4e13c3831392ab6c499385338 Uploaded 20170531 diff -r 81faa1567178 -r 565118df598a ._ETn_example Binary file ._ETn_example has changed diff -r 81faa1567178 -r 565118df598a ._example Binary file ._example has changed diff -r 81faa1567178 -r 565118df598a ._loadandplot.R Binary file ._loadandplot.R has changed diff -r 81faa1567178 -r 565118df598a ._plotwithscale.R Binary file ._plotwithscale.R has changed diff -r 81faa1567178 -r 565118df598a ._testandplot.R Binary file ._testandplot.R has changed diff -r 81faa1567178 -r 565118df598a ETn_example/._.DS_Store Binary file ETn_example/._.DS_Store has changed diff -r 81faa1567178 -r 565118df598a ETn_example/._Control.bed Binary file ETn_example/._Control.bed has changed diff -r 81faa1567178 -r 565118df598a ETn_example/._DESCRIPTION.txt Binary file ETn_example/._DESCRIPTION.txt has changed diff -r 81faa1567178 -r 565118df598a ETn_example/._ETn_fixed.bed Binary file ETn_example/._ETn_fixed.bed has changed diff -r 81faa1567178 -r 565118df598a ETn_example/._Recombination_hotspots.txt Binary file ETn_example/._Recombination_hotspots.txt has changed diff -r 81faa1567178 -r 565118df598a ETn_example/._features.header Binary file ETn_example/._features.header has changed diff -r 81faa1567178 -r 565118df598a ETn_example/._regions.header Binary file ETn_example/._regions.header has changed diff -r 81faa1567178 -r 565118df598a ETn_example/Control.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ETn_example/Control.bed Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,1142 @@ +chr1 6743847 6807847 +chr1 12755253 12819253 +chr1 14459704 14523704 +chr1 16989370 17053370 +chr1 18859600 18923600 +chr1 20393735 20457735 +chr1 20457735 20521735 +chr1 22357071 22421071 +chr1 34267805 34331805 +chr1 36860915 36924915 +chr1 38084729 38148729 +chr1 38513351 38577351 +chr1 39997553 40061553 +chr1 40484791 40548791 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The region dataset "Control" contains 1142 regions of 64 kb without elements, +used as control in the test. The regions are aligned around their center (i.e. around the ETn integration +sites). +Recombination hotspots measurements are associated to each "ETn fixed" and "Control" region. In +particular, this feature is measured in 1-kb windows, so that each region is associated to a recombination +hotspots curve made of 64 values. The measurement used is the feature content, i.e. the +fraction of the 1-kb window that is covered by recombination hotspots + +Data have been collected and pre-processed by: R Campos-Sanchez, MA Cremona, A Pini, F +Chiaromonte and KD Makova (2016). Integration and fixation preferences of human and mouse +endogenous retroviruses uncovered with Functional Data Analysis. PLoS Computational Biology. +12(6): 1-41. +Fixed ETn positions come from: Y Zhang, IA Maksakova, L Gagnier, LN van de Lagemaat, DL +Mager (2008). Genome-wide assessments reveal extremely high levels of polymorphism of two +active families of mouse endogenous retroviral elements. PLoS Genetics. 4: e1000007. +Recombination hotspots data come from: H Brunschwig, L Levi, E Ben-David, RW Williams, +B Yakir, S Shifman (2012). Fine-scale maps of recombination rates and hotspots in the mouse +genome. Genetics. 191: 757-764. \ No newline at end of file diff -r 81faa1567178 -r 565118df598a ETn_example/ETn_fixed.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ETn_example/ETn_fixed.bed Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,1296 @@ +chr1 3079711 3144047 +chr1 3232230 3302517 +chr1 3756792 3826331 +chr1 4589320 4653381 +chr1 5155380 5219717 +chr1 5634836 5704314 +chr1 5967107 6035506 +chr1 6240632 6304964 +chr1 7186756 7250816 +chr1 7434671 7504179 +chr1 7573651 7643108 +chr1 7879930 7944250 +chr1 7962100 8026423 +chr1 8219414 8283736 +chr1 9451579 9515898 +chr1 10561964 10626025 +chr1 10774950 10844464 +chr1 11301539 11365867 +chr1 11479858 11543919 +chr1 11577435 11646975 +chr1 11734130 11798451 +chr1 12053425 12117744 +chr1 12503733 12568058 +chr1 12690915 12755253 +chr1 13015768 13080108 +chr1 13772979 13840932 +chr1 14114086 14178424 +chr1 15752556 15816879 +chr1 15820454 15889959 +chr1 16894874 16964379 +chr1 17180480 17250022 +chr1 17380662 17444982 +chr1 17626574 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-0,0 +1,1 @@ +Recombination_hotspots.txt Recomb_hot Recombination hotspots \ No newline at end of file diff -r 81faa1567178 -r 565118df598a ETn_example/regions.header --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ETn_example/regions.header Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,2 @@ +Control.bed Control Control +ETn_fixed.bed ETn_fixed ETn fixed \ No newline at end of file diff -r 81faa1567178 -r 565118df598a example/._.DS_Store Binary file example/._.DS_Store has changed diff -r 81faa1567178 -r 565118df598a example/._Controls_regions.bed Binary file example/._Controls_regions.bed has changed diff -r 81faa1567178 -r 565118df598a example/._DESCRIPTION.txt Binary file example/._DESCRIPTION.txt has changed diff -r 81faa1567178 -r 565118df598a example/._Elements1_regions.bed Binary file example/._Elements1_regions.bed has changed diff -r 81faa1567178 -r 565118df598a example/._Elements2_regions.bed Binary file example/._Elements2_regions.bed has changed diff -r 81faa1567178 -r 565118df598a example/._Elements3_regions.bed Binary file example/._Elements3_regions.bed has changed diff -r 81faa1567178 -r 565118df598a example/._Feature1.bed Binary file example/._Feature1.bed has changed diff -r 81faa1567178 -r 565118df598a example/._Feature2.bed Binary file example/._Feature2.bed has changed diff -r 81faa1567178 -r 565118df598a example/._features.header.bed.txt Binary file example/._features.header.bed.txt has changed diff -r 81faa1567178 -r 565118df598a example/._regions.header.txt Binary file example/._regions.header.txt has changed diff -r 81faa1567178 -r 565118df598a example/Controls_regions.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example/Controls_regions.bed Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,35 @@ +chr1 91399892 91499892 +chr1 116447079 116547079 +chr2 231381109 231481109 +chr3 47386633 47486633 +chr3 99195712 99295712 +chr3 116765114 116865114 +chr3 134345017 134445017 +chr4 68064019 68164019 +chr4 137465811 137565811 +chr4 173293016 173393016 +chr5 59044110 59144110 +chr5 94592763 94692763 +chr5 108436006 108536006 +chr5 110836898 110936898 +chr5 146661073 146761073 +chr6 99727306 99827306 +chr7 88291325 88391325 +chr7 120999784 121099784 +chr8 126860366 126960366 +chr8 127636547 127736547 +chr11 41362564 41462564 +chr11 101741527 101841527 +chr12 15210277 15310277 +chr13 91106037 91206037 +chr13 91518822 91618822 +chr13 98959030 99059030 +chr15 39087176 39187176 +chr17 58542142 58642142 +chr18 12199823 12299823 +chr20 8173704 8273704 +chr21 16255112 16355112 +chr21 45089115 45189115 +chrX 23898983 23998983 +chrX 37029585 37129585 +chrX 103593349 103693349 diff -r 81faa1567178 -r 565118df598a example/DESCRIPTION.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example/DESCRIPTION.txt Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,2 @@ +This example contains simulated measurements of two different genomic features, "ftr1" and "ftr2", +corresponding to four different region datasets: "elem1", "elem2", "elem3" and "control". diff -r 81faa1567178 -r 565118df598a example/Elements1_regions.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example/Elements1_regions.bed Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,25 @@ +chr2 49960150 50060150 +chr2 55912445 56012445 +chr3 140326750 140426750 +chr4 41772201 41872201 +chr4 70629719 70729719 +chr4 109340735 109440735 +chr5 15025389 15125389 +chr5 43028888 43128888 +chr5 58231354 58331354 +chr5 91277761 91377761 +chr5 119464508 119564508 +chr6 85309170 85409170 +chr6 85762072 85862072 +chr6 134621363 134721363 +chr6 147470164 147570164 +chr7 77195798 77295798 +chr7 77880914 77980914 +chr7 114805399 114905399 +chr10 46350885 46450885 +chr10 67833078 67933078 +chr12 27722692 27822692 +chr12 95518506 95618506 +chr12 117863466 117963466 +chr14 100464166 100564166 +chrX 29266748 29366748 diff -r 81faa1567178 -r 565118df598a example/Elements2_regions.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example/Elements2_regions.bed Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,20 @@ +chr1 65942901 66042901 +chr1 117128102 117228102 +chr1 190338169 190438169 +chr2 227365478 227465478 +chr6 50856764 50956764 +chr6 135703067 135803067 +chr7 38228726 38328726 +chr7 51115898 51215898 +chr7 150325760 150425760 +chr8 25475691 25575691 +chr9 73374609 73474609 +chr11 55092625 55192625 +chr11 86581661 86681661 +chr12 12795284 12895284 +chr12 48151882 48251882 +chr12 83109951 83209951 +chr15 31430278 31530278 +chr15 65610815 65710815 +chr17 40766223 40866223 +chr20 17245048 17345048 diff -r 81faa1567178 -r 565118df598a example/Elements3_regions.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example/Elements3_regions.bed Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,28 @@ +chr1 49721616 49821616 +chr1 95059425 95159425 +chr1 207236313 207336313 +chr2 73790843 73890843 +chr3 134465321 134565321 +chr4 43433669 43533669 +chr4 122032777 122132777 +chr5 12701747 12801747 +chr5 58501529 58601529 +chr5 152853250 152953250 +chr6 136085023 136185023 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example/regions.header.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/example/regions.header.txt Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,4 @@ +Elements1_regions.bed elem1 Elements 1 +Elements2_regions.bed elem2 Elements 2 +Elements3_regions.bed elem3 Elements 3 +Controls_regions.bed control Controls \ No newline at end of file diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/._.DS_Store Binary file iwtomics/ETn_example/._.DS_Store has changed diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/._Control.bed Binary file iwtomics/ETn_example/._Control.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/._DESCRIPTION.txt Binary file iwtomics/ETn_example/._DESCRIPTION.txt has changed diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/._ETn_fixed.bed Binary file iwtomics/ETn_example/._ETn_fixed.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/._Recombination_hotspots.txt Binary file 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113836504 113900504 -chr9 118910538 118974538 -chr9 121627136 121691136 diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/DESCRIPTION.txt --- a/iwtomics/ETn_example/DESCRIPTION.txt Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,21 +0,0 @@ -This example contains two region datasets "ETn fixed", "Control" and one feature "Recombination hotspots content". -In particular, the region dataset "ETn fixed" contains 1296 genomic regions of 64 kb surrounding -fixed ETns elements (32-kb flanking sequences upstream and 32-kb flanking sequences downstream -of each element). The region dataset "Control" contains 1142 regions of 64 kb without elements, -used as control in the test. The regions are aligned around their center (i.e. around the ETn integration -sites). -Recombination hotspots measurements are associated to each "ETn fixed" and "Control" region. In -particular, this feature is measured in 1-kb windows, so that each region is associated to a recombination -hotspots curve made of 64 values. The measurement used is the feature content, i.e. the -fraction of the 1-kb window that is covered by recombination hotspots - -Data have been collected and pre-processed by: R Campos-Sanchez, MA Cremona, A Pini, F -Chiaromonte and KD Makova (2016). Integration and fixation preferences of human and mouse -endogenous retroviruses uncovered with Functional Data Analysis. PLoS Computational Biology. -12(6): 1-41. -Fixed ETn positions come from: Y Zhang, IA Maksakova, L Gagnier, LN van de Lagemaat, DL -Mager (2008). Genome-wide assessments reveal extremely high levels of polymorphism of two -active families of mouse endogenous retroviral elements. PLoS Genetics. 4: e1000007. -Recombination hotspots data come from: H Brunschwig, L Levi, E Ben-David, RW Williams, -B Yakir, S Shifman (2012). Fine-scale maps of recombination rates and hotspots in the mouse -genome. Genetics. 191: 757-764. \ No newline at end of file diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/ETn_fixed.bed --- a/iwtomics/ETn_example/ETn_fixed.bed Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1296 +0,0 @@ -chr1 3079711 3144047 -chr1 3232230 3302517 -chr1 3756792 3826331 -chr1 4589320 4653381 -chr1 5155380 5219717 -chr1 5634836 5704314 -chr1 5967107 6035506 -chr1 6240632 6304964 -chr1 7186756 7250816 -chr1 7434671 7504179 -chr1 7573651 7643108 -chr1 7879930 7944250 -chr1 7962100 8026423 -chr1 8219414 8283736 -chr1 9451579 9515898 -chr1 10561964 10626025 -chr1 10774950 10844464 -chr1 11301539 11365867 -chr1 11479858 11543919 -chr1 11577435 11646975 -chr1 11734130 11798451 -chr1 12053425 12117744 -chr1 12503733 12568058 -chr1 12690915 12755253 -chr1 13015768 13080108 -chr1 13772979 13840932 -chr1 14114086 14178424 -chr1 15752556 15816879 -chr1 15820454 15889959 -chr1 16894874 16964379 -chr1 17180480 17250022 -chr1 17380662 17444982 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0 0 0 0 0 0 0.82 1 1 1 1 1 1 1 1 1 1 0.18 0 0 0 0 0 0.82 1 1 1 0.18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.82 -chr9 107848560 107912560 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 1 0.27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -chr9 107941738 108005738 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.908 1 0.092 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -chr9 108683437 108747437 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -chr9 108962296 109026296 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.466 1 1 1 0.534 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -chr9 109947901 110011901 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -chr9 110485254 110549254 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -chr9 110616374 110680374 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.544 1 1 1 1 1 1 1 1 1 0.456 0.544 1 1 1 1 1 1 0.456 0.544 1 1 0.456 0.544 1 1 1 -chr9 111968818 112032818 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -chr9 113836504 113900504 0 0 0.457 1 1 1 1 0.543 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.457 1 1 1 1 1 1 1 1 0.543 0 0 0 0 0.457 1 1 1 1 -chr9 118910538 118974538 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.491 1 1 1 1 1 1 1 1 0.509 0 0 0 0 0 0 0 0 0 0 -chr9 121627136 121691136 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/features.header --- a/iwtomics/ETn_example/features.header Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1 +0,0 @@ -Recombination_hotspots.txt Recomb_hot Recombination hotspots \ No newline at end of file diff -r 81faa1567178 -r 565118df598a iwtomics/ETn_example/regions.header --- a/iwtomics/ETn_example/regions.header Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -Control.bed Control Control -ETn_fixed.bed ETn_fixed ETn fixed \ No newline at end of file diff -r 81faa1567178 -r 565118df598a iwtomics/example/._.DS_Store Binary file iwtomics/example/._.DS_Store has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._Controls_regions.bed Binary file iwtomics/example/._Controls_regions.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._DESCRIPTION.txt Binary file iwtomics/example/._DESCRIPTION.txt has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._Elements1_regions.bed Binary file iwtomics/example/._Elements1_regions.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._Elements2_regions.bed Binary file iwtomics/example/._Elements2_regions.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._Elements3_regions.bed Binary file iwtomics/example/._Elements3_regions.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._Feature1.bed Binary file iwtomics/example/._Feature1.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._Feature2.bed Binary file iwtomics/example/._Feature2.bed has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._features.header.bed.txt Binary file iwtomics/example/._features.header.bed.txt has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/._regions.header.txt Binary file iwtomics/example/._regions.header.txt has changed diff -r 81faa1567178 -r 565118df598a iwtomics/example/Controls_regions.bed --- a/iwtomics/example/Controls_regions.bed Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,35 +0,0 @@ -chr1 91399892 91499892 -chr1 116447079 116547079 -chr2 231381109 231481109 -chr3 47386633 47486633 -chr3 99195712 99295712 -chr3 116765114 116865114 -chr3 134345017 134445017 -chr4 68064019 68164019 -chr4 137465811 137565811 -chr4 173293016 173393016 -chr5 59044110 59144110 -chr5 94592763 94692763 -chr5 108436006 108536006 -chr5 110836898 110936898 -chr5 146661073 146761073 -chr6 99727306 99827306 -chr7 88291325 88391325 -chr7 120999784 121099784 -chr8 126860366 126960366 -chr8 127636547 127736547 -chr11 41362564 41462564 -chr11 101741527 101841527 -chr12 15210277 15310277 -chr13 91106037 91206037 -chr13 91518822 91618822 -chr13 98959030 99059030 -chr15 39087176 39187176 -chr17 58542142 58642142 -chr18 12199823 12299823 -chr20 8173704 8273704 -chr21 16255112 16355112 -chr21 45089115 45189115 -chrX 23898983 23998983 -chrX 37029585 37129585 -chrX 103593349 103693349 diff -r 81faa1567178 -r 565118df598a iwtomics/example/DESCRIPTION.txt --- a/iwtomics/example/DESCRIPTION.txt Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -This example contains simulated measurements of two different genomic features, "ftr1" and "ftr2", -corresponding to four different region datasets: "elem1", "elem2", "elem3" and "control". diff -r 81faa1567178 -r 565118df598a iwtomics/example/Elements1_regions.bed --- a/iwtomics/example/Elements1_regions.bed Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,25 +0,0 @@ -chr2 49960150 50060150 -chr2 55912445 56012445 -chr3 140326750 140426750 -chr4 41772201 41872201 -chr4 70629719 70729719 -chr4 109340735 109440735 -chr5 15025389 15125389 -chr5 43028888 43128888 -chr5 58231354 58331354 -chr5 91277761 91377761 -chr5 119464508 119564508 -chr6 85309170 85409170 -chr6 85762072 85862072 -chr6 134621363 134721363 -chr6 147470164 147570164 -chr7 77195798 77295798 -chr7 77880914 77980914 -chr7 114805399 114905399 -chr10 46350885 46450885 -chr10 67833078 67933078 -chr12 27722692 27822692 -chr12 95518506 95618506 -chr12 117863466 117963466 -chr14 100464166 100564166 -chrX 29266748 29366748 diff -r 81faa1567178 -r 565118df598a iwtomics/example/Elements2_regions.bed --- a/iwtomics/example/Elements2_regions.bed Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,20 +0,0 @@ -chr1 65942901 66042901 -chr1 117128102 117228102 -chr1 190338169 190438169 -chr2 227365478 227465478 -chr6 50856764 50956764 -chr6 135703067 135803067 -chr7 38228726 38328726 -chr7 51115898 51215898 -chr7 150325760 150425760 -chr8 25475691 25575691 -chr9 73374609 73474609 -chr11 55092625 55192625 -chr11 86581661 86681661 -chr12 12795284 12895284 -chr12 48151882 48251882 -chr12 83109951 83209951 -chr15 31430278 31530278 -chr15 65610815 65710815 -chr17 40766223 40866223 -chr20 17245048 17345048 diff -r 81faa1567178 -r 565118df598a iwtomics/example/Elements3_regions.bed --- a/iwtomics/example/Elements3_regions.bed Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,28 +0,0 @@ -chr1 49721616 49821616 -chr1 95059425 95159425 -chr1 207236313 207336313 -chr2 73790843 73890843 -chr3 134465321 134565321 -chr4 43433669 43533669 -chr4 122032777 122132777 -chr5 12701747 12801747 -chr5 58501529 58601529 -chr5 152853250 152953250 -chr6 136085023 136185023 -chr6 147019529 147119529 -chr7 14192964 14292964 -chr7 125519883 125619883 -chr8 77652872 77752872 -chr8 108375665 108475665 -chr9 2984971 3084971 -chr10 978822 1078822 -chr11 18395046 18495046 -chr12 47273592 47373592 -chr12 60095778 60195778 -chr12 84854096 84954096 -chr12 95157539 95257539 -chr14 23982000 24082000 -chr16 1875335 1975335 -chr16 66789863 66889863 -chr18 64644131 64744131 -chr21 15966609 16066609 diff -r 81faa1567178 -r 565118df598a iwtomics/example/Feature1.bed --- a/iwtomics/example/Feature1.bed Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,5400 +0,0 @@ -chr1 91399892 91401892 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7.3992370168724 -chr21 16060609 16062609 7.97487587946482 -chr21 16062609 16064609 6.76355670816454 -chr21 16064609 16066609 6.11742019669001 diff -r 81faa1567178 -r 565118df598a iwtomics/example/features.header.bed.txt --- a/iwtomics/example/features.header.bed.txt Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -Feature1.bed ftr1 Feature 1 -Feature2.bed ftr2 Feature 2 \ No newline at end of file diff -r 81faa1567178 -r 565118df598a iwtomics/example/regions.header.txt --- a/iwtomics/example/regions.header.txt Wed May 31 11:06:30 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,4 +0,0 @@ -Elements1_regions.bed elem1 Elements 1 -Elements2_regions.bed elem2 Elements 2 -Elements3_regions.bed elem3 Elements 3 -Controls_regions.bed control Controls \ No newline at end of file diff -r 81faa1567178 -r 565118df598a loadandplot.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/loadandplot.R Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,146 @@ +if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { + require(tools,quietly = FALSE) + args=commandArgs(TRUE) + + for (i in seq_along(args)) { + message(args[[i]]) + } + + # get args names and values + args_values=strsplit(args,'=') + args_names=unlist(lapply(args_values,function(arg) arg[1])) + names(args_values)=args_names + args_values=lapply(args_values,function(arg) arg[2]) + # read filenames + outrdata=args_values$outrdata + outregions=args_values$outregions + outfeatures=args_values$outfeatures + outpdf=args_values$outpdf + regionspaths=unlist(strsplit(args_values$regionspaths,'\\|')) + if("regionsheaderfile" %in% args_names){ + # the file regionsheaderfile must contain as first column the (unique) regionsfilenames, + # as second column the corresponding ids and as third column the names + tryCatch({ + regionsheader=read.delim(args_values$regionsheaderfile,header=FALSE,stringsAsFactors=FALSE,row.names=1,sep="\t") + regionsfilenames=unlist(strsplit(args_values$regionsfilenames,'\\|')) + if(length(setdiff(regionsfilenames,row.names(regionsheader)))) { + quit(save="no", status=11) + stop('Not all regionsfilenames are present in the first column of regionsheader.') + } + id_regions=regionsheader[regionsfilenames,1] + name_regions=regionsheader[regionsfilenames,2] + }, error = function(err) { + quit(save="no", status=10) #error on header file + stop(err) + }) + }else{ + eval(parse(text=args[[which(args_names=='regionsgalaxyids')]])) + id_regions=paste0('data_',regionsgalaxyids) + name_regions=paste0('data_',regionsgalaxyids) + } + featurespaths=unlist(strsplit(args_values$featurespaths,'\\|')) + if("featuresheaderfile" %in% args_names){ + # the file featuresheaderfile must contain as first column the (unique) featuresfilenames, + # as second column the corresponding ids and as third column the names + tryCatch({ + featuresheader=read.delim(args_values$featuresheaderfile,header=FALSE,stringsAsFactors=FALSE,row.names=1,sep="\t") + featuresfilenames=unlist(strsplit(args_values$featuresfilenames,'\\|')) + if(length(setdiff(featuresfilenames,row.names(featuresheader)))) { + quit(save="no", status=21) + stop('Not all featuresfilenames are present in the first column of featuresheader.') + } + id_features=featuresheader[featuresfilenames,1] + name_features=featuresheader[featuresfilenames,2] + }, error = function(err) { + quit(save="no", status=20) #error on header file + stop(err) + }) + }else{ + eval(parse(text=args[[which(args_names=='featuresgalaxyids')]])) + id_features=paste0('data_',featuresgalaxyids) + name_features=paste0('data_',featuresgalaxyids) + } + # read parameters (from smoothing on) + i_smoothing=which(args_names=='smoothing') + for(i in i_smoothing:length(args)){ + eval(parse(text=args[[i]])) + } + + # load data + tryCatch({ + regionsFeatures=IWTomicsData(regionspaths,featurespaths,alignment, + id_regions,name_regions,id_features,name_features,start.are.0based=start.are.0based) + }, error = function(err) { + if(grepl('invalid format',err$message)){ + quit(save="no", status=31) # error, not enough columns in input file + }else if(grepl('duplicated regions',err$message)){ + quit(save="no", status=32) # error, duplicated regions in region file + }else if(grepl('duplicated windows',err$message)){ + quit(save="no", status=33) # error, duplicated windows in feature file + }else if(grepl('overlapping windows',err$message)){ + quit(save="no", status=34) # error, overlapping windows in feature file + }else if(grepl('not all regions in datasets',err$message)){ + quit(save="no", status=35) # error, windows in feature files do not cover all regions in region files + }else if(grepl('ifferent size windows',err$message)){ + quit(save="no", status=36) # error, all windows in a feature files must have the same size + } + #error loading data + + stop(err) + + quit(save="no", status=30) + + }) + + # smooth data + if(smoothing!='no'){ + tryCatch({ + if(smoothing=='locpoly'){ + dist_knots=10 + }else if(smoothing=='kernel'){ + degree=3 + dist_knots=10 + }else if(smoothing=='splines'){ + bandwidth=5 + } + if(alignment=='scale'){ + if(scale==0){ + regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, + bandwidth=bandwidth,degree=degree,dist_knots=dist_knots) + }else{ + regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, + bandwidth=bandwidth,degree=degree,dist_knots=dist_knots,scale_grid=scale) + } + }else{ + regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, + bandwidth=bandwidth,degree=degree,dist_knots=dist_knots) + } + }, error = function(err) { + quit(save="no", status=40) #error on smoothing + stop(err) + }) + } + + # plot data + pdf(outpdf,width=10,height=8) + if(plottype=='boxplot'){ + # fix repeated probs + probs=sort(unique(probs)) + }else{ + probs=c(0.25,0.5,0.75) + } + plot(regionsFeatures,type=plottype,probs=probs,average=average,size=size,ask=FALSE) + dev.off() + + # create output + #write.table(cbind(unlist(strsplit(args_values$regionsfilenames,'\\|')),idRegions(regionsFeatures),nameRegions(regionsFeatures)), + #file=outregions,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + write.table(as.data.frame(t(idRegions(regionsFeatures))),file=outregions,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + #write.table(cbind(unlist(strsplit(args_values$featuresfilenames,'\\|')),idFeatures(regionsFeatures),nameFeatures(regionsFeatures)), + #file=outfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + write.table(as.data.frame(t(idFeatures(regionsFeatures))),file=outfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + save(regionsFeatures,file=outrdata) +}else{ + quit(save="no", status=255) + stop("Missing IWTomics package") +} \ No newline at end of file diff -r 81faa1567178 -r 565118df598a loadandplot.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/loadandplot.xml Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,325 @@ + + Smooth and Plot + + macros.xml + + + + + + + + + + + + + + + + + + + + +& /dev/null +]]> + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
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+ + + + + + + + + + +This tool imports a collection of genomic region datasets, and associates to each region +multiple genomic feature measurements. It allows to align the regions in multiple ways +(center, left, right or scale alignment), to smooth the feature curves (possibly filling gaps in the +measurements) and to create a graphical representation of the feature measurements in each +region datasets (aligned curves or pointwise quantile curves). + +----- + +**Region datasets** + +Each region dataset can be provided as a BED or Tabular file with tab delimited columns chr +start end (extra columns present in the input file are ignored). Regions can be of different +length:: + + chr2 49960150 50060150 + chr2 55912445 56012445 + ... + +----- + +**Feature measurements** + +Feature measurements corresponding to all the regions can be provided as a BED or Tabular +file with tab delimited columns chr start end value:: + + chr2 49960150 49962150 0.9426 + chr2 49962150 49964150 0.7816 + ... + +Each feature must be measured in windows of a fixed size inside all the regions (missing +values must be indicated as NA). Another way to import feature measurements is from a +Tabular file with the first three columns chr start end corresponding to the different genomic +regions, followed on the same row by all the measurements in fixed-size windows:: + + chr2 49960150 50060150 0.9426 0.7816 0.8921 ... ... 1.2063 + chr2 55912445 56012445 0.8719 0.9975 1.1619 ... ... 0.9601 + ... + +----- + +**Output** + +The tool returns: + +1. RData with the IWTomicsData object, that stores the aligned genomic region datasets, and their associated feature measurements; +2. Region dataset identifiers; +3. Feature identifiers; +4. PDF file with the plotted data. + +1-3 can be used as input of the tool *IWTomics Test and Plot* + +----- + +.. class:: infomark + +**Notes** + +This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). + +It implements a simplified version of the methods *smooth* and *plot* for *IWTomicsData* objects. +The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). + +.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf + + + + + @ARTICLE{ + iwt-functional-data, + author = {Pini, Alessia and Vantini, Simone}, + title = {Interval-Wise Testing for functional data}, + journal = {Journal of Nonparametric Statistics}, + year = {2017}, + volume = {29}, + number = {2}, + pages = {407-424} + } + + + @MANUAL{ + iwtomics, + author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, + title = {IWTomics: Interval-Wise Testing for Omics Data}, + note = {R package version 0.99.12}, + year = {2017} + } + + + +
diff -r 81faa1567178 -r 565118df598a macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,9 @@ + + 1.0.0 + + + R + bioconductor-iwtomics + + + \ No newline at end of file diff -r 81faa1567178 -r 565118df598a plotwithscale.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plotwithscale.R Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,117 @@ +if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { + args=commandArgs(TRUE) + + # get args names and values + args_values=strsplit(args,'=') + args_names=unlist(lapply(args_values,function(arg) arg[1])) + names(args_values)=args_names + args_values=lapply(args_values,function(arg) arg[2]) + # read filenames + adjustedpvalue=args_values$adjustedpvalue + iwtomicsrespdf=args_values$iwtomicsrespdf + iwtomicssumpdf=args_values$iwtomicssumpdf + iwtomicsrdata=args_values$iwtomicsrdata + iwtomicstests=args_values$iwtomicstests + iwtomicsselectedfeatures=args_values$iwtomicsselectedfeatures + test_subset=paste0('c(',strsplit(args_values$test_subset,'\\|')[[1]],')') + feature_subset=paste0('c(',strsplit(args_values$feature_subset,'\\|')[[1]],')') + # read parameters (from test_subset on) + i_scale_subset=which(args_names=='scale_subset') + for(i in i_scale_subset:length(args)){ + eval(parse(text=args[[i]])) + } + + # load RData + load(iwtomicsrdata) + # read testids and featureids and check them + unlisted=lapply(seq_along(test_subset), + function(i){ + test_subset_i=eval(parse(text=test_subset[i])) + feature_subset_i=eval(parse(text=feature_subset[i])) + test_subset_i=rep(test_subset_i,each=length(feature_subset_i)) + feature_subset_i=rep(feature_subset_i,length.out=length(test_subset_i)) + scale_subset_i=rep(scale_subset[i],length(test_subset_i)) + return(list(test_subset=test_subset_i,feature_subset=feature_subset_i,scale_subset=scale_subset_i)) + }) + test_subset=unlist(lapply(unlisted,function(l) l$test_subset)) + feature_subset=unlist(lapply(unlisted,function(l) l$feature_subset)) + scale_subset=unlist(lapply(unlisted,function(l) l$scale_subset)) + testids=as.character(read.delim(iwtomicstests,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + featureids=as.character(read.delim(iwtomicsselectedfeatures,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + id_features_subset=featureids[feature_subset] + if(sum(testids!=paste(testInput(regionsFeatures_test)$id_region1,'vs',testInput(regionsFeatures_test)$id_region2))){ + quit(save="no", status=10) + stop('Wrong test ids') + } + if(sum(featureids!=idFeatures(regionsFeatures_test))){ + quit(save="no", status=20) + stop('Wrong feature ids') + } + # retrieve test and features_subset ids + id_features_subset=featureids[feature_subset] + if(sum(duplicated(paste0(test_subset,id_features_subset)))){ + quit(save="no", status=30) + stop('Two scale thresholds selected for the same test and feature.') + } + # If scale_subset=0, do not change the threshold + default=(scale_subset==0) + scale_subset=scale_subset[!default] + test_subset=test_subset[!default] + id_features_subset=id_features_subset[!default] + + # get scale threshold + scale_threshold=lapply(regionsFeatures_test@test$result, + function(result) unlist(lapply(result,function(feature) feature$max_scale))) + for(i in seq_along(test_subset)){ + if(scale_threshold[[test_subset[i]]][id_features_subset[i]] + on Test Scale + + macros.xml + + + + + + + + + + + + + + +& /dev/null +]]> + + + + + + ... + + + + + + + + + + +
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+ + + + + + + + + +This tool allows to select the scale for the Interval-Wise Testing results. In particular, it returns the p-value curves +for the different tests performed at the selected scale, and it creates a graphical representation of the +Interval-Wise Testing results and a summary plot (optional) at the selected scale. + +----- + +**Input files** + +RData file with the IWTomicsData object with test results, tabular files with test IDs and feature IDs. +These files are created by the tool *IWTomics Test and Plot*. + +----- + +**Output** + +The tool returns: + +1. TXT file with an adjusted p-value curve for every test performed at the selected scale; +2. PDF file with the plotted test results; +3. PDF file with the summary plot. + +----- + +.. class:: infomark + +**Notes** + +This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). + +It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects. +The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). + +.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf + + + + + @ARTICLE{ + iwt-functional-data, + author = {A Pini and S. Vantini}, + title = {Interval-Wise Testing for functional data}, + journal = {Journal of Nonparametric Statistics}, + year = {2017}, + volume = {29}, + number = {2}, + pages = {407-424} + } + + + @MANUAL{ + iwtomics, + author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, + title = {IWTomics: Interval-Wise Testing for Omics Data}, + note = {R package version 0.99.12}, + year = {2017} + } + + + + diff -r 81faa1567178 -r 565118df598a testandplot.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/testandplot.R Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,99 @@ +if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { + args=commandArgs(TRUE) + + # get args names and values + args_values=strsplit(args,'=') + args_names=unlist(lapply(args_values,function(arg) arg[1])) + names(args_values)=args_names + args_values=lapply(args_values,function(arg) arg[2]) + # read filenames + adjustedpvaluematrix=args_values$adjustedpvaluematrix + iwtomicsrespdf=args_values$iwtomicsrespdf + iwtomicssumpdf=args_values$iwtomicssumpdf + regionids=args_values$regionids + featureids=args_values$featureids + rdatafile=args_values$rdatafile + iwtomicsrdata=args_values$iwtomicsrdata + iwtomicstests=args_values$iwtomicstests + iwtomicsselectedfeatures=args_values$iwtomicsselectedfeatures + # read parameters (from region1 on) + i_region1=which(args_names=='region1') + for(i in i_region1:length(args)){ + eval(parse(text=args[[i]])) + } + + # load RData + load(rdatafile) + # read regionids and featureids + regionids=as.character(read.delim(regionids,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + featureids=as.character(read.delim(featureids,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + # retrieve region1, region2 and features_subset ids and check they are in the RData + id_region1=regionids[region1] + id_region2=regionids[region2] + id_features_subset=featureids[features_subset] + if(length(setdiff(c(id_region1,id_region2),idRegions(regionsFeatures)))!=0){ + quit(save="no", status=10) + stop('Wrong region ids') + } + if(length(setdiff(id_features_subset,idFeatures(regionsFeatures)))!=0){ + quit(save="no", status=20) + stop('Wrong feature ids') + } + if(sum(duplicated(paste0(id_region1,id_region2)))){ + quit(save="no", status=30) + stop('Same test repeated multiple times.') + } + + # perform test + tryCatch({ + # fix repeated probs + if(statistics=='quantile'){ + # fix repeated probs + testprobs=sort(unique(testprobs)) + }else{ + testprobs=0.5 + } + regionsFeatures_test=IWTomicsTest(regionsFeatures,id_region1,id_region2,id_features_subset, + statistics=statistics,probs=testprobs,B=B) + # create adjustedvaluematrix output + for(test in seq_along(id_region1)){ + for(id_feature in id_features_subset){ + write(paste0('Test: ',id_region1[test],' vs ',id_region2[test],', on feature ',id_feature), + file=adjustedpvaluematrix,append=TRUE,sep='\t') + pval=regionsFeatures_test@test$result[[test]][[id_feature]]$adjusted_pval_matrix + row.names(pval)=paste('Scale',rev(seq_len(nrow(pval)))) + write.table(pval,file=adjustedpvaluematrix,append=TRUE,sep='\t',quote=FALSE,row.names=TRUE,col.names=FALSE) + write('',file=adjustedpvaluematrix,append=TRUE,sep='\t') + } + } + }, error = function(err) { + quit(save="no", status=40) #error testing + stop(err) + }) + + # plot test results + pdf(iwtomicsrespdf,width=5,height=7) + if(plottype=='boxplot'){ + # fix repeated probs + probs=sort(unique(probs)) + }else{ + probs=c(0.25,0.5,0.75) + } + plotTest(regionsFeatures_test,alpha=testalpha,type=plottype,probs=probs,average=average,size=size,ask=FALSE) + dev.off() + + # plot summary results + if(groupby!='none'){ + pdf(iwtomicssumpdf,width=15,height=10) + plotSummary(regionsFeatures_test,alpha=summaryalpha,only_significant=only_significant,groupby=groupby,ask=FALSE,append=TRUE) + dev.off() + } + + # create output + write.table(as.data.frame(t(paste(id_region1,'vs',id_region2))),file=iwtomicstests,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + write.table(as.data.frame(t(idFeatures(regionsFeatures_test))),file=iwtomicsselectedfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + save(regionsFeatures_test,file=iwtomicsrdata) +}else{ + quit(save="no", status=255) + stop("Missing IWTomics package") +} \ No newline at end of file diff -r 81faa1567178 -r 565118df598a testandplot.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/testandplot.xml Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,239 @@ + + and Plot + + macros.xml + + + + + + + + + + + + +& /dev/null +]]> + + + + + + + + + + + + +
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+ + + + + + + + + + + + +This tool statistically evaluates differences in genomic features between groups of regions along the genome. +In particular, it implements the Interval-Wise Testing for omics data, an extended version of the Interval-Wise +Testing for functional data presented in Pini and Vantini (2017). It allows to perform multiple two sample permutation +tests between pairs of region datasets, on several features. It returns the adjusted p-value curves for every test +and all possible scales. Moreover, it creates a graphical representation of the Interval-Wise Testing results +and a summary plot (optional) with p-values at the maximum scale. The tool *IWTomics Plot with Threshold on Test Scale* +permits to select the scale to be used in the plots. + +----- + +**Input files** + +RData file with the IWTomicsData object, tabular files with region dataset IDs and feature IDs. +These files are created by the tool *IWTomics Load Smooth and Plot*. + +----- + +**Output** + +The tool returns: + +1. TXT file with an adjusted p-value matrix for every test performed. Each matrix contains a p-value curve (row) for every scale considered in the test; +2. PDF file with the plotted test results; +3. PDF file with the summary plot; +4. RData with the IWTomicsData object with the test results; +5. Test identifiers; +6. Feature identifiers. + +4-6 can be used as input of the tool *IWTomics Plot with Threshold on Test Scale* + +----- + +.. class:: infomark + +**Notes** + +This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). + +It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects. +The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). + +.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf + + + + + @ARTICLE{ + iwt-functional-data, + author = {A Pini and S. Vantini}, + title = {Interval-Wise Testing for functional data}, + journal = {Journal of Nonparametric Statistics}, + year = {2017}, + volume = {29}, + number = {2}, + pages = {407-424} + } + + + @MANUAL{ + iwtomics, + author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, + title = {IWTomics: Interval-Wise Testing for Omics Data}, + note = {R package version 0.99.12}, + year = {2017} + } + + + +