Mercurial > repos > fabio > iwtomics
changeset 16:ed9594c25fad draft
Uploaded 20170531
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--- a/ETn_example/DESCRIPTION.txt Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,21 +0,0 @@ -This example contains two region datasets "ETn fixed", "Control" and one feature "Recombination hotspots content". -In particular, the region dataset "ETn fixed" contains 1296 genomic regions of 64 kb surrounding -fixed ETns elements (32-kb flanking sequences upstream and 32-kb flanking sequences downstream -of each element). The region dataset "Control" contains 1142 regions of 64 kb without elements, -used as control in the test. The regions are aligned around their center (i.e. around the ETn integration -sites). -Recombination hotspots measurements are associated to each "ETn fixed" and "Control" region. In -particular, this feature is measured in 1-kb windows, so that each region is associated to a recombination -hotspots curve made of 64 values. The measurement used is the feature content, i.e. the -fraction of the 1-kb window that is covered by recombination hotspots - -Data have been collected and pre-processed by: R Campos-Sanchez, MA Cremona, A Pini, F -Chiaromonte and KD Makova (2016). Integration and fixation preferences of human and mouse -endogenous retroviruses uncovered with Functional Data Analysis. PLoS Computational Biology. -12(6): 1-41. -Fixed ETn positions come from: Y Zhang, IA Maksakova, L Gagnier, LN van de Lagemaat, DL -Mager (2008). Genome-wide assessments reveal extremely high levels of polymorphism of two -active families of mouse endogenous retroviral elements. PLoS Genetics. 4: e1000007. -Recombination hotspots data come from: H Brunschwig, L Levi, E Ben-David, RW Williams, -B Yakir, S Shifman (2012). Fine-scale maps of recombination rates and hotspots in the mouse -genome. Genetics. 191: 757-764. \ No newline at end of file
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--- a/ETn_example/features.header Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1 +0,0 @@ -Recombination_hotspots.txt Recomb_hot Recombination hotspots \ No newline at end of file
--- a/ETn_example/regions.header Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -Control.bed Control Control -ETn_fixed.bed ETn_fixed ETn fixed \ No newline at end of file
--- a/README.txt Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,3 +0,0 @@ -This tool requires the following dependencies: -1. R (https://cran.r-project.org/) -2. R/Bioconductor package IWTomics (https://bioconductor.org/packages/release/bioc/html/IWTomics.html)
--- a/example/Controls_regions.bed Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,35 +0,0 @@ -chr1 91399892 91499892 -chr1 116447079 116547079 -chr2 231381109 231481109 -chr3 47386633 47486633 -chr3 99195712 99295712 -chr3 116765114 116865114 -chr3 134345017 134445017 -chr4 68064019 68164019 -chr4 137465811 137565811 -chr4 173293016 173393016 -chr5 59044110 59144110 -chr5 94592763 94692763 -chr5 108436006 108536006 -chr5 110836898 110936898 -chr5 146661073 146761073 -chr6 99727306 99827306 -chr7 88291325 88391325 -chr7 120999784 121099784 -chr8 126860366 126960366 -chr8 127636547 127736547 -chr11 41362564 41462564 -chr11 101741527 101841527 -chr12 15210277 15310277 -chr13 91106037 91206037 -chr13 91518822 91618822 -chr13 98959030 99059030 -chr15 39087176 39187176 -chr17 58542142 58642142 -chr18 12199823 12299823 -chr20 8173704 8273704 -chr21 16255112 16355112 -chr21 45089115 45189115 -chrX 23898983 23998983 -chrX 37029585 37129585 -chrX 103593349 103693349
--- a/example/DESCRIPTION.txt Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -This example contains simulated measurements of two different genomic features, "ftr1" and "ftr2", -corresponding to four different region datasets: "elem1", "elem2", "elem3" and "control".
--- a/example/Elements1_regions.bed Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,25 +0,0 @@ -chr2 49960150 50060150 -chr2 55912445 56012445 -chr3 140326750 140426750 -chr4 41772201 41872201 -chr4 70629719 70729719 -chr4 109340735 109440735 -chr5 15025389 15125389 -chr5 43028888 43128888 -chr5 58231354 58331354 -chr5 91277761 91377761 -chr5 119464508 119564508 -chr6 85309170 85409170 -chr6 85762072 85862072 -chr6 134621363 134721363 -chr6 147470164 147570164 -chr7 77195798 77295798 -chr7 77880914 77980914 -chr7 114805399 114905399 -chr10 46350885 46450885 -chr10 67833078 67933078 -chr12 27722692 27822692 -chr12 95518506 95618506 -chr12 117863466 117963466 -chr14 100464166 100564166 -chrX 29266748 29366748
--- a/example/Elements2_regions.bed Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,20 +0,0 @@ -chr1 65942901 66042901 -chr1 117128102 117228102 -chr1 190338169 190438169 -chr2 227365478 227465478 -chr6 50856764 50956764 -chr6 135703067 135803067 -chr7 38228726 38328726 -chr7 51115898 51215898 -chr7 150325760 150425760 -chr8 25475691 25575691 -chr9 73374609 73474609 -chr11 55092625 55192625 -chr11 86581661 86681661 -chr12 12795284 12895284 -chr12 48151882 48251882 -chr12 83109951 83209951 -chr15 31430278 31530278 -chr15 65610815 65710815 -chr17 40766223 40866223 -chr20 17245048 17345048
--- a/example/Elements3_regions.bed Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,28 +0,0 @@ -chr1 49721616 49821616 -chr1 95059425 95159425 -chr1 207236313 207336313 -chr2 73790843 73890843 -chr3 134465321 134565321 -chr4 43433669 43533669 -chr4 122032777 122132777 -chr5 12701747 12801747 -chr5 58501529 58601529 -chr5 152853250 152953250 -chr6 136085023 136185023 -chr6 147019529 147119529 -chr7 14192964 14292964 -chr7 125519883 125619883 -chr8 77652872 77752872 -chr8 108375665 108475665 -chr9 2984971 3084971 -chr10 978822 1078822 -chr11 18395046 18495046 -chr12 47273592 47373592 -chr12 60095778 60195778 -chr12 84854096 84954096 -chr12 95157539 95257539 -chr14 23982000 24082000 -chr16 1875335 1975335 -chr16 66789863 66889863 -chr18 64644131 64744131 -chr21 15966609 16066609
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--- a/example/features.header.bed.txt Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ -Feature1.bed ftr1 Feature 1 -Feature2.bed ftr2 Feature 2 \ No newline at end of file
--- a/example/regions.header.txt Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,4 +0,0 @@ -Elements1_regions.bed elem1 Elements 1 -Elements2_regions.bed elem2 Elements 2 -Elements3_regions.bed elem3 Elements 3 -Controls_regions.bed control Controls \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/.Rhistory Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,3 @@ +require(IWTomics) +require(pheatmap) +install.packages(pheatmap)
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/ETn_example/DESCRIPTION.txt Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,21 @@ +This example contains two region datasets "ETn fixed", "Control" and one feature "Recombination hotspots content". +In particular, the region dataset "ETn fixed" contains 1296 genomic regions of 64 kb surrounding +fixed ETns elements (32-kb flanking sequences upstream and 32-kb flanking sequences downstream +of each element). The region dataset "Control" contains 1142 regions of 64 kb without elements, +used as control in the test. The regions are aligned around their center (i.e. around the ETn integration +sites). +Recombination hotspots measurements are associated to each "ETn fixed" and "Control" region. In +particular, this feature is measured in 1-kb windows, so that each region is associated to a recombination +hotspots curve made of 64 values. The measurement used is the feature content, i.e. the +fraction of the 1-kb window that is covered by recombination hotspots + +Data have been collected and pre-processed by: R Campos-Sanchez, MA Cremona, A Pini, F +Chiaromonte and KD Makova (2016). Integration and fixation preferences of human and mouse +endogenous retroviruses uncovered with Functional Data Analysis. PLoS Computational Biology. +12(6): 1-41. +Fixed ETn positions come from: Y Zhang, IA Maksakova, L Gagnier, LN van de Lagemaat, DL +Mager (2008). Genome-wide assessments reveal extremely high levels of polymorphism of two +active families of mouse endogenous retroviral elements. PLoS Genetics. 4: e1000007. +Recombination hotspots data come from: H Brunschwig, L Levi, E Ben-David, RW Williams, +B Yakir, S Shifman (2012). Fine-scale maps of recombination rates and hotspots in the mouse +genome. Genetics. 191: 757-764. \ No newline at end of file
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/ETn_example/features.header Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,1 @@ +Recombination_hotspots.txt Recomb_hot Recombination hotspots \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/ETn_example/regions.header Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,2 @@ +Control.bed Control Control +ETn_fixed.bed ETn_fixed ETn fixed \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/Controls_regions.bed Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,35 @@ +chr1 91399892 91499892 +chr1 116447079 116547079 +chr2 231381109 231481109 +chr3 47386633 47486633 +chr3 99195712 99295712 +chr3 116765114 116865114 +chr3 134345017 134445017 +chr4 68064019 68164019 +chr4 137465811 137565811 +chr4 173293016 173393016 +chr5 59044110 59144110 +chr5 94592763 94692763 +chr5 108436006 108536006 +chr5 110836898 110936898 +chr5 146661073 146761073 +chr6 99727306 99827306 +chr7 88291325 88391325 +chr7 120999784 121099784 +chr8 126860366 126960366 +chr8 127636547 127736547 +chr11 41362564 41462564 +chr11 101741527 101841527 +chr12 15210277 15310277 +chr13 91106037 91206037 +chr13 91518822 91618822 +chr13 98959030 99059030 +chr15 39087176 39187176 +chr17 58542142 58642142 +chr18 12199823 12299823 +chr20 8173704 8273704 +chr21 16255112 16355112 +chr21 45089115 45189115 +chrX 23898983 23998983 +chrX 37029585 37129585 +chrX 103593349 103693349
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/DESCRIPTION.txt Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,2 @@ +This example contains simulated measurements of two different genomic features, "ftr1" and "ftr2", +corresponding to four different region datasets: "elem1", "elem2", "elem3" and "control".
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/Elements1_regions.bed Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,25 @@ +chr2 49960150 50060150 +chr2 55912445 56012445 +chr3 140326750 140426750 +chr4 41772201 41872201 +chr4 70629719 70729719 +chr4 109340735 109440735 +chr5 15025389 15125389 +chr5 43028888 43128888 +chr5 58231354 58331354 +chr5 91277761 91377761 +chr5 119464508 119564508 +chr6 85309170 85409170 +chr6 85762072 85862072 +chr6 134621363 134721363 +chr6 147470164 147570164 +chr7 77195798 77295798 +chr7 77880914 77980914 +chr7 114805399 114905399 +chr10 46350885 46450885 +chr10 67833078 67933078 +chr12 27722692 27822692 +chr12 95518506 95618506 +chr12 117863466 117963466 +chr14 100464166 100564166 +chrX 29266748 29366748
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/Elements2_regions.bed Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,20 @@ +chr1 65942901 66042901 +chr1 117128102 117228102 +chr1 190338169 190438169 +chr2 227365478 227465478 +chr6 50856764 50956764 +chr6 135703067 135803067 +chr7 38228726 38328726 +chr7 51115898 51215898 +chr7 150325760 150425760 +chr8 25475691 25575691 +chr9 73374609 73474609 +chr11 55092625 55192625 +chr11 86581661 86681661 +chr12 12795284 12895284 +chr12 48151882 48251882 +chr12 83109951 83209951 +chr15 31430278 31530278 +chr15 65610815 65710815 +chr17 40766223 40866223 +chr20 17245048 17345048
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/Elements3_regions.bed Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,28 @@ +chr1 49721616 49821616 +chr1 95059425 95159425 +chr1 207236313 207336313 +chr2 73790843 73890843 +chr3 134465321 134565321 +chr4 43433669 43533669 +chr4 122032777 122132777 +chr5 12701747 12801747 +chr5 58501529 58601529 +chr5 152853250 152953250 +chr6 136085023 136185023 +chr6 147019529 147119529 +chr7 14192964 14292964 +chr7 125519883 125619883 +chr8 77652872 77752872 +chr8 108375665 108475665 +chr9 2984971 3084971 +chr10 978822 1078822 +chr11 18395046 18495046 +chr12 47273592 47373592 +chr12 60095778 60195778 +chr12 84854096 84954096 +chr12 95157539 95257539 +chr14 23982000 24082000 +chr16 1875335 1975335 +chr16 66789863 66889863 +chr18 64644131 64744131 +chr21 15966609 16066609
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/Feature1.bed Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,5400 @@ +chr1 91399892 91401892 0.914729028492568 +chr1 91401892 91403892 1.03788669317729 +chr1 91403892 91405892 1.15695078809331 +chr1 91405892 91407892 1.05433971377051 +chr1 91407892 91409892 1.05827244724317 +chr1 91409892 91411892 1.00216117481117 +chr1 91411892 91413892 0.873489001753337 +chr1 91413892 91415892 0.873100412116323 +chr1 91415892 91417892 0.928712683217515 +chr1 91417892 91419892 0.94132562114723 +chr1 91419892 91421892 1.06739894033636 +chr1 91421892 91423892 0.909275169317382 +chr1 91423892 91425892 1.10093887463398 +chr1 91425892 91427892 1.05342912580018 +chr1 91427892 91429892 0.970074712768588 +chr1 91429892 91431892 0.742552329847752 +chr1 91431892 91433892 1.09649292566553 +chr1 91433892 91435892 0.902578291528885 +chr1 91435892 91437892 0.821718064516058 +chr1 91437892 91439892 0.909063175846949 +chr1 91439892 91441892 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/features.header.bed.txt Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,2 @@ +Feature1.bed ftr1 Feature 1 +Feature2.bed ftr2 Feature 2 \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/example/regions.header.txt Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,4 @@ +Elements1_regions.bed elem1 Elements 1 +Elements2_regions.bed elem2 Elements 2 +Elements3_regions.bed elem3 Elements 3 +Controls_regions.bed control Controls \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/loadandplot.R Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,146 @@ +if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { + require(tools,quietly = FALSE) + args=commandArgs(TRUE) + + for (i in seq_along(args)) { + message(args[[i]]) + } + + # get args names and values + args_values=strsplit(args,'=') + args_names=unlist(lapply(args_values,function(arg) arg[1])) + names(args_values)=args_names + args_values=lapply(args_values,function(arg) arg[2]) + # read filenames + outrdata=args_values$outrdata + outregions=args_values$outregions + outfeatures=args_values$outfeatures + outpdf=args_values$outpdf + regionspaths=unlist(strsplit(args_values$regionspaths,'\\|')) + if("regionsheaderfile" %in% args_names){ + # the file regionsheaderfile must contain as first column the (unique) regionsfilenames, + # as second column the corresponding ids and as third column the names + tryCatch({ + regionsheader=read.delim(args_values$regionsheaderfile,header=FALSE,stringsAsFactors=FALSE,row.names=1,sep="\t") + regionsfilenames=unlist(strsplit(args_values$regionsfilenames,'\\|')) + if(length(setdiff(regionsfilenames,row.names(regionsheader)))) { + quit(save="no", status=11) + stop('Not all regionsfilenames are present in the first column of regionsheader.') + } + id_regions=regionsheader[regionsfilenames,1] + name_regions=regionsheader[regionsfilenames,2] + }, error = function(err) { + quit(save="no", status=10) #error on header file + stop(err) + }) + }else{ + eval(parse(text=args[[which(args_names=='regionsgalaxyids')]])) + id_regions=paste0('data_',regionsgalaxyids) + name_regions=paste0('data_',regionsgalaxyids) + } + featurespaths=unlist(strsplit(args_values$featurespaths,'\\|')) + if("featuresheaderfile" %in% args_names){ + # the file featuresheaderfile must contain as first column the (unique) featuresfilenames, + # as second column the corresponding ids and as third column the names + tryCatch({ + featuresheader=read.delim(args_values$featuresheaderfile,header=FALSE,stringsAsFactors=FALSE,row.names=1,sep="\t") + featuresfilenames=unlist(strsplit(args_values$featuresfilenames,'\\|')) + if(length(setdiff(featuresfilenames,row.names(featuresheader)))) { + quit(save="no", status=21) + stop('Not all featuresfilenames are present in the first column of featuresheader.') + } + id_features=featuresheader[featuresfilenames,1] + name_features=featuresheader[featuresfilenames,2] + }, error = function(err) { + quit(save="no", status=20) #error on header file + stop(err) + }) + }else{ + eval(parse(text=args[[which(args_names=='featuresgalaxyids')]])) + id_features=paste0('data_',featuresgalaxyids) + name_features=paste0('data_',featuresgalaxyids) + } + # read parameters (from smoothing on) + i_smoothing=which(args_names=='smoothing') + for(i in i_smoothing:length(args)){ + eval(parse(text=args[[i]])) + } + + # load data + tryCatch({ + regionsFeatures=IWTomicsData(regionspaths,featurespaths,alignment, + id_regions,name_regions,id_features,name_features,start.are.0based=start.are.0based) + }, error = function(err) { + if(grepl('invalid format',err$message)){ + quit(save="no", status=31) # error, not enough columns in input file + }else if(grepl('duplicated regions',err$message)){ + quit(save="no", status=32) # error, duplicated regions in region file + }else if(grepl('duplicated windows',err$message)){ + quit(save="no", status=33) # error, duplicated windows in feature file + }else if(grepl('overlapping windows',err$message)){ + quit(save="no", status=34) # error, overlapping windows in feature file + }else if(grepl('not all regions in datasets',err$message)){ + quit(save="no", status=35) # error, windows in feature files do not cover all regions in region files + }else if(grepl('ifferent size windows',err$message)){ + quit(save="no", status=36) # error, all windows in a feature files must have the same size + } + #error loading data + + stop(err) + + quit(save="no", status=30) + + }) + + # smooth data + if(smoothing!='no'){ + tryCatch({ + if(smoothing=='locpoly'){ + dist_knots=10 + }else if(smoothing=='kernel'){ + degree=3 + dist_knots=10 + }else if(smoothing=='splines'){ + bandwidth=5 + } + if(alignment=='scale'){ + if(scale==0){ + regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, + bandwidth=bandwidth,degree=degree,dist_knots=dist_knots) + }else{ + regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, + bandwidth=bandwidth,degree=degree,dist_knots=dist_knots,scale_grid=scale) + } + }else{ + regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, + bandwidth=bandwidth,degree=degree,dist_knots=dist_knots) + } + }, error = function(err) { + quit(save="no", status=40) #error on smoothing + stop(err) + }) + } + + # plot data + pdf(outpdf,width=10,height=8) + if(plottype=='boxplot'){ + # fix repeated probs + probs=sort(unique(probs)) + }else{ + probs=c(0.25,0.5,0.75) + } + plot(regionsFeatures,type=plottype,probs=probs,average=average,size=size,ask=FALSE) + dev.off() + + # create output + #write.table(cbind(unlist(strsplit(args_values$regionsfilenames,'\\|')),idRegions(regionsFeatures),nameRegions(regionsFeatures)), + #file=outregions,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + write.table(as.data.frame(t(idRegions(regionsFeatures))),file=outregions,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + #write.table(cbind(unlist(strsplit(args_values$featuresfilenames,'\\|')),idFeatures(regionsFeatures),nameFeatures(regionsFeatures)), + #file=outfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + write.table(as.data.frame(t(idFeatures(regionsFeatures))),file=outfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + save(regionsFeatures,file=outrdata) +}else{ + quit(save="no", status=255) + stop("Missing IWTomics package") +} \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/loadandplot.xml Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,325 @@ +<tool id="loadandplot" name="IWTomics Load" version="@VERSION@.0"> + <description>Smooth and Plot</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <stdio> + <exit_code range="255" source="both" level="fatal" description="Missing IWTomics package. Please be sure to have it installed before using this tool." /> + <exit_code range="10" source="both" level="fatal" description="An error has occurred reading the header file for regions. Please try again." /> + <exit_code range="11" source="both" level="fatal" description="Not all region files are present in the first column of header file for regions." /> + <exit_code range="20" source="both" level="fatal" description="An error has occurred reading the header file for features. Please try again." /> + <exit_code range="21" source="both" level="fatal" description="Not all feature files are present in the first column of header file for features." /> + <exit_code range="30" source="both" level="fatal" description="An error has occurred reading the data. Please try again." /> + <exit_code range="31" source="both" level="fatal" description="Not enough columns in input file." /> + <exit_code range="32" source="both" level="fatal" description="Duplicated regions in region file." /> + <exit_code range="33" source="both" level="fatal" description="Duplicated windows in feature file." /> + <exit_code range="34" source="both" level="fatal" description="Overlapping windows in feature file." /> + <exit_code range="35" source="both" level="fatal" description="Windows in feature files do not cover all regions in region files." /> + <exit_code range="36" source="both" level="fatal" description="All windows in a feature file must have the same size." /> + <exit_code range="40" source="both" level="fatal" description="An error has occurred smoothing the data. Please try again." /> + <regex match="Error" source="both" level="fatal" description="An error has occurred. Please try again." /> + </stdio> + + <command> +<![CDATA[ + Rscript $__tool_directory__/loadandplot.R outrdata="${outrdata}" outregions="${outregions}" outfeatures="${outfeatures}" outpdf="${outpdf}" + + #set region_paths = '"\\|"'.join( [ str( $r ) for $r in $regions ] ) + regionspaths="${region_paths}" + #set region_galaxyids = '","'.join( [ str( $r.hid ) for $r in $regions ] ) + regionsgalaxyids="c(${region_galaxyids})" + #set region_names = '"\\|"'.join( [ str( $r.name ) for $r in $regions ] ) + regionsfilenames="${region_names}" + #set region_header = '"\\|"'.join( [ str( $rh.regionsheader ) for $rh in $regionsheaderrepeat ] ) + #if $region_header != "": + regionsheaderfile="${region_header}" + #end if + + #set feature_paths = '"\\|"'.join( [ str( $f ) for $f in $features ] ) + featurespaths="${feature_paths}" + #set feature_galaxyids = '","'.join( [ str( $f.hid ) for $f in $features ] ) + featuresgalaxyids="c(${feature_galaxyids})" + #set feature_names = '"\\|"'.join( [ str( $f.name ) for $f in $features ] ) + featuresfilenames="${feature_names}" + #set feature_header = '"\\|"'.join( [ str( $fh.featuresheader ) for $fh in $featuresheaderrepeat ] ) + #if $feature_header != "": + featuresheaderfile="${feature_header}" + #end if + + smoothing="'${conditionaltype.smoothing}'" + start.are.0based="${zerobased}" + #if $conditionaltype.smoothing == 'no': + alignment="'${conditionaltype.alignment}'" + #elif $conditionaltype.smoothing == 'locpoly': + alignment="'${conditionaltype.locpolyconditionalscale.alignment}'" + #if $conditionaltype.locpolyconditionalscale.alignment == 'scale': + scale="${conditionaltype.locpolyconditionalscale.scalegrid}" + #end if + bandwidth="${conditionaltype.locpolybandwidth}" + degree="${conditionaltype.locpolydegree}" + fill_gaps="${conditionaltype.fillgaps}" + #elif $conditionaltype.smoothing == 'kernel': + alignment="'${conditionaltype.kernelconditionalscale.alignment}'" + #if $conditionaltype.kernelconditionalscale.alignment == 'scale': + scale="${conditionaltype.kernelconditionalscale.scalegrid}" + #end if + bandwidth="${conditionaltype.kernelbandwidth}" + fill_gaps="${conditionaltype.fillgaps}" + #elif $conditionaltype.smoothing == 'splines': + alignment="'${conditionaltype.splinesconditionalscale.alignment}'" + #if $conditionaltype.splinesconditionalscale.alignment == 'scale': + scale="${conditionaltype.splinesconditionalscale.scalegrid}" + #end if + degree="${conditionaltype.splinesdegree}" + dist_knots="${conditionaltype.splinesdistknots}" + fill_gaps="${conditionaltype.fillgaps}" + #end if + + average="${plotres.average}" + size="${plotres.size}" + plottype="'${plotres.conditionalplottype.plottype}'" + #if $plotres.conditionalplottype.plottype == 'boxplot': + #set probs = '","'.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] ) + #if $probs != "": + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})" + #else: + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})" + #end if + #end if + + >& /dev/null +]]> + <!-- + to print the stack add the following line at the end of the command and enable the corresponding entry in output + 2> "${stackerr}" + --> + </command> + + <inputs> + <!-- regions --> + <param format="tabular" name="regions" type="data" label="Select region files" multiple="true" help="" /> + <repeat name="regionsheaderrepeat" title="Header file for regions (optional)" min="0" max="1" help="Tabular file. Column 1: names of the region files; Column 2: unique identifiers of the region datasets (spaces and special characters are not allowed); Column 3: name of the region datasets to be used in the output."> + <param format="tabular" name="regionsheader" type="data" label="Select header file" /> + </repeat> + + <!-- features --> + <param format="tabular" name="features" type="data" label="Select feature files" multiple="true" help="" /> + <repeat name="featuresheaderrepeat" title="Header file for features (optional)" min="0" max="1" help="Tabular file. Column 1: names of the feature files; Column 2: unique identifiers of the features (spaces and special characters are not allowed); Column 3: name of the features to be used in the output."> + <param format="tabular" name="featuresheader" type="data" label="Select header file" /> + </repeat> + + <!-- zero-base one-base --> + <param name="zerobased" type="select" label="Start positions are:" multiple="false" help=""> + <option value="TRUE">0-based</option> + <option value="FALSE">1-based</option> + </param> + + <!-- conditional smoothing --> + <conditional name="conditionaltype"> + <!-- smoothing --> + <param name="smoothing" type="select" label="Smoothing" help="Type of smoothing to be applied to the feature curves."> + <option value="no">No</option> + <option value="locpoly">Local Polynomials</option> + <option value="kernel">Gaussian Kernel</option> + <option value="splines">B-Splines</option> + </param> + <!-- conditional choice: smoothing=no --> + <when value="no"> + <param name="alignment" type="select" label="Region alignment" help=""> + <option value="center">Center - Alignment on the central position</option> + <option value="left">Left - Alignment on the starting position</option> + <option value="right">Right - Alignment on the ending position</option> + </param> + </when> + <!-- conditional choice: smoothing=locpoly --> + <when value="locpoly"> + <!-- conditional region alignment --> + <conditional name="locpolyconditionalscale"> + <param name="alignment" type="select" label="Region alignment" help=""> + <option value="center">Center - Alignment on the central position</option> + <option value="left">Left - Alignment on the starting position</option> + <option value="right">Right - Alignment on the ending position</option> + <option value="scale">Scale - Scaling all regions to the same length</option> + </param> + <when value="scale"> + <param name="scalegrid" type="integer" value="0" min="0" label="Number of grid points" help="Number of equally-spaced grid points over which the smoothed curves are evaluated." /> + </when> + </conditional> + + <param name="locpolybandwidth" type="integer" value="5" min="2" label="Bandwidth for smoothing" help="" /> + <param name="locpolydegree" type="integer" value="3" min="0" label="Degree of local polynomial" help="" /> + + <!-- fill gaps --> + <param name="fillgaps" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Use smoothing to fill gaps (NA measurements)" help="" /> + </when> + <!-- conditional choice: smoothing=kernel --> + <when value="kernel"> + <!-- conditional region alignment --> + <conditional name="kernelconditionalscale"> + <param name="alignment" type="select" label="Region alignment" help=""> + <option value="center">Center - Alignment on the central position</option> + <option value="left">Left - Alignment on the starting position</option> + <option value="right">Right - Alignment on the ending position</option> + <option value="scale">Scale - Scaling all regions to the same length</option> + </param> + <when value="scale"> + <param name="scalegrid" type="integer" value="0" min="0" label="Number of grid points" help="Number of equally-spaced grid points over which the smoothed curves are evaluated." /> + </when> + </conditional> + + <param name="kernelbandwidth" type="integer" value="5" min="2" label="Bandwidth for smoothing" help="" /> + + <!-- fill gaps --> + <param name="fillgaps" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Use smoothing to fill gaps (NA measurements)" help="" /> + </when> + <!-- conditional choice: smoothing=splines --> + <when value="splines"> + <!-- conditional region alignment --> + <conditional name="splinesconditionalscale"> + <param name="alignment" type="select" label="Region alignment" help=""> + <option value="center">Center - Alignment on the central position</option> + <option value="left">Left - Alignment on the starting position</option> + <option value="right">Right - Alignment on the ending position</option> + <option value="scale">Scale - Scaling all regions to the same length</option> + </param> + <when value="scale"> + <param name="scalegrid" type="integer" value="0" min="0" label="Number of grid points" help="Number of equally-spaced grid points over which the smoothed curves are evaluated." /> + </when> + </conditional> + + <param name="splinesdegree" type="integer" value="3" min="0" label="Degree of B-splines" help="" /> + <param name="splinesdistknots" type="integer" value="10" min="2" label="Distance between nodes (approximate)" help="" /> + + <!-- fill gaps --> + <param name="fillgaps" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Use smoothing to fill gaps (NA measurements)" help="" /> + </when> + </conditional> + + <!-- plot IWTomics results --> + <section name="plotres" title="Plot data" expanded="True"> + <!-- average --> + <param name="average" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot the mean curves" help="" /> + <!-- sample size in each position --> + <param name="size" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot sample size in each position" help="" /> + <!-- conditional plot type --> + <conditional name="conditionalplottype"> + <!-- plot type --> + <param name="plottype" type="select" label="Plot type" help=""> + <option value="boxplot">Pointwise quantile curves (boxplot)</option> + <option value="curves">Curves (aligned)</option> + </param> + <!-- conditional choice: plottype=boxplot --> + <when value="boxplot"> + <section name="probabilitiessection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantile curves to be drawn."> + <param name="prob0" size="3" type="float" value="0.25" min="0.0" max="1.0" label="Probability" /> + <param name="prob1" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + <param name="prob2" size="3" type="float" value="0.75" min="0.0" max="1.0" label="Probability" /> + <repeat name="probabilities" title="Probabilities"> + <param name="prob" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + </repeat> + </section> + </when> + </conditional> + </section> + </inputs> + + <outputs> + <!--<data format="txt" name="stackerr" label="iwtomics.loadandplot.stackerr.txt" from_work_dir="iwtomics.loadandplot.stackerr.txt" />--> + <data format="rdata" name="outrdata" label="${tool.name} on ${on_string}: IWTomicsData Object" from_work_dir="iwtomics.loadandplot.RData" /> + <data format="tabular" name="outregions" label="${tool.name} on ${on_string}: Region Dataset IDs" from_work_dir="iwtomics.loadandplot.regions.txt" /> + <data format="tabular" name="outfeatures" label="${tool.name} on ${on_string}: Feature IDs" from_work_dir="iwtomics.loadandplot.features.txt" /> + <data format="pdf" name="outpdf" label="${tool.name} on ${on_string}: Plotted Data" from_work_dir="iwtomics.loadandplot.pdf" /> + </outputs> + + <help> +This tool imports a collection of genomic region datasets, and associates to each region +multiple genomic feature measurements. It allows to align the regions in multiple ways +(center, left, right or scale alignment), to smooth the feature curves (possibly filling gaps in the +measurements) and to create a graphical representation of the feature measurements in each +region datasets (aligned curves or pointwise quantile curves). + +----- + +**Region datasets** + +Each region dataset can be provided as a BED or Tabular file with tab delimited columns chr +start end (extra columns present in the input file are ignored). Regions can be of different +length:: + + chr2 49960150 50060150 + chr2 55912445 56012445 + ... + +----- + +**Feature measurements** + +Feature measurements corresponding to all the regions can be provided as a BED or Tabular +file with tab delimited columns chr start end value:: + + chr2 49960150 49962150 0.9426 + chr2 49962150 49964150 0.7816 + ... + +Each feature must be measured in windows of a fixed size inside all the regions (missing +values must be indicated as NA). Another way to import feature measurements is from a +Tabular file with the first three columns chr start end corresponding to the different genomic +regions, followed on the same row by all the measurements in fixed-size windows:: + + chr2 49960150 50060150 0.9426 0.7816 0.8921 ... ... 1.2063 + chr2 55912445 56012445 0.8719 0.9975 1.1619 ... ... 0.9601 + ... + +----- + +**Output** + +The tool returns: + +1. RData with the IWTomicsData object, that stores the aligned genomic region datasets, and their associated feature measurements; +2. Region dataset identifiers; +3. Feature identifiers; +4. PDF file with the plotted data. + +1-3 can be used as input of the tool *IWTomics Test and Plot* + +----- + +.. class:: infomark + +**Notes** + +This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). + +It implements a simplified version of the methods *smooth* and *plot* for *IWTomicsData* objects. +The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). + +.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf + </help> + + <citations> + <citation type="bibtex"> + @ARTICLE{ + iwt-functional-data, + author = {Pini, Alessia and Vantini, Simone}, + title = {Interval-Wise Testing for functional data}, + journal = {Journal of Nonparametric Statistics}, + year = {2017}, + volume = {29}, + number = {2}, + pages = {407-424} + } + </citation> + <citation type="bibtex"> + @MANUAL{ + iwtomics, + author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, + title = {IWTomics: Interval-Wise Testing for Omics Data}, + note = {R package version 0.99.12}, + year = {2017} + } + </citation> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/macros.xml Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,9 @@ +<macros> + <token name="@VERSION@">1.0.0</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="3.3.1">R</requirement> + <requirement type="package" version="@VERSION">bioconductor-iwtomics</requirement> + </requirements> + </xml> +</macros> \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/plotwithscale.R Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,117 @@ +if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { + args=commandArgs(TRUE) + + # get args names and values + args_values=strsplit(args,'=') + args_names=unlist(lapply(args_values,function(arg) arg[1])) + names(args_values)=args_names + args_values=lapply(args_values,function(arg) arg[2]) + # read filenames + adjustedpvalue=args_values$adjustedpvalue + iwtomicsrespdf=args_values$iwtomicsrespdf + iwtomicssumpdf=args_values$iwtomicssumpdf + iwtomicsrdata=args_values$iwtomicsrdata + iwtomicstests=args_values$iwtomicstests + iwtomicsselectedfeatures=args_values$iwtomicsselectedfeatures + test_subset=paste0('c(',strsplit(args_values$test_subset,'\\|')[[1]],')') + feature_subset=paste0('c(',strsplit(args_values$feature_subset,'\\|')[[1]],')') + # read parameters (from test_subset on) + i_scale_subset=which(args_names=='scale_subset') + for(i in i_scale_subset:length(args)){ + eval(parse(text=args[[i]])) + } + + # load RData + load(iwtomicsrdata) + # read testids and featureids and check them + unlisted=lapply(seq_along(test_subset), + function(i){ + test_subset_i=eval(parse(text=test_subset[i])) + feature_subset_i=eval(parse(text=feature_subset[i])) + test_subset_i=rep(test_subset_i,each=length(feature_subset_i)) + feature_subset_i=rep(feature_subset_i,length.out=length(test_subset_i)) + scale_subset_i=rep(scale_subset[i],length(test_subset_i)) + return(list(test_subset=test_subset_i,feature_subset=feature_subset_i,scale_subset=scale_subset_i)) + }) + test_subset=unlist(lapply(unlisted,function(l) l$test_subset)) + feature_subset=unlist(lapply(unlisted,function(l) l$feature_subset)) + scale_subset=unlist(lapply(unlisted,function(l) l$scale_subset)) + testids=as.character(read.delim(iwtomicstests,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + featureids=as.character(read.delim(iwtomicsselectedfeatures,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + id_features_subset=featureids[feature_subset] + if(sum(testids!=paste(testInput(regionsFeatures_test)$id_region1,'vs',testInput(regionsFeatures_test)$id_region2))){ + quit(save="no", status=10) + stop('Wrong test ids') + } + if(sum(featureids!=idFeatures(regionsFeatures_test))){ + quit(save="no", status=20) + stop('Wrong feature ids') + } + # retrieve test and features_subset ids + id_features_subset=featureids[feature_subset] + if(sum(duplicated(paste0(test_subset,id_features_subset)))){ + quit(save="no", status=30) + stop('Two scale thresholds selected for the same test and feature.') + } + # If scale_subset=0, do not change the threshold + default=(scale_subset==0) + scale_subset=scale_subset[!default] + test_subset=test_subset[!default] + id_features_subset=id_features_subset[!default] + + # get scale threshold + scale_threshold=lapply(regionsFeatures_test@test$result, + function(result) unlist(lapply(result,function(feature) feature$max_scale))) + for(i in seq_along(test_subset)){ + if(scale_threshold[[test_subset[i]]][id_features_subset[i]]<scale_subset[i]){ + quit(save="no", status=40) + stop('Scale threshold too high.') + } + scale_threshold[[test_subset[i]]][id_features_subset[i]]=scale_subset[i] + } + + # create adjustedvalue output + pval=adjusted_pval(regionsFeatures_test,scale_threshold=scale_threshold) + for(test in seq_along(pval)){ + for(id_feature in idFeatures(regionsFeatures_test)){ + write(paste0('Test: ',testids[test],', on feature ',id_feature), + file=adjustedpvalue,append=TRUE,sep='\t') + pval_i=as.data.frame(t(pval[[test]][[id_feature]])) + row.names(pval_i)=paste('Scale',scale_threshold[[test]][[id_feature]]) + write.table(pval_i,file=adjustedpvalue,append=TRUE,sep='\t',quote=FALSE,row.names=TRUE,col.names=FALSE) + write('',file=adjustedpvalue,append=TRUE,sep='\t') + } + } + + + # plot test results + pdf(iwtomicsrespdf,width=5,height=7) + if(plottype=='boxplot'){ + # fix repeated probs + probs=sort(unique(probs)) + }else{ + probs=c(0.25,0.5,0.75) + } + plotTest(regionsFeatures_test,alpha=testalpha,type=plottype,probs=probs,average=average,size=size,scale_threshold=scale_threshold,ask=FALSE) + dev.off() + + # plot summary results + if(groupby!='none'){ + tryCatch({ + pdf(iwtomicssumpdf,width=15,height=10) + plotSummary(regionsFeatures_test,alpha=summaryalpha,only_significant=only_significant,groupby=groupby,scale_threshold=scale_threshold,ask=FALSE,append=TRUE) + dev.off() + }, error = function(err) { + if (grepl('selected features with different resolution',err$message)) { + quit(save="no", status=50) #error: groupby 'test' but selected features with different resolution. + stop(err) + } + quit(save="no", status=60) #error + stop(err) + }) + } + +}else{ + quit(save="no", status=255) + stop("Missing IWTomics package") +} \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/plotwithscale.xml Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,201 @@ +<tool id="plotwithscale" name="IWTomics Plot with Threshold" version="@VERSION@.0"> + <description>on Test Scale</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <stdio> + <exit_code range="-1" source="both" level="fatal" description="Missing IWTomics package. Please be sure to have it installed before using this tool." /> + <exit_code range="10" source="both" level="fatal" description="Wrong test ids." /> + <exit_code range="20" source="both" level="fatal" description="Wrong feature ids." /> + <exit_code range="30" source="both" level="fatal" description="Two scale thresholds selected for the same test and feature." /> + <exit_code range="40" source="both" level="fatal" description="Scale threshold too high." /> + <exit_code range="50" source="both" level="fatal" description="Group by 'test' but selected features with different resolution." /> + <exit_code range="60" source="both" level="fatal" description="Summary plot error. Please try again." /> + <regex match="Error" source="both" level="fatal" description="An error has occurred. Please try again." /> + </stdio> + + <command> +<![CDATA[ + Rscript $__tool_directory__/plotwithscale.R adjustedpvalue="${adjustedpvalue}" iwtomicsrespdf="${iwtomicsrespdf}" iwtomicssumpdf="${iwtomicssumpdf}" + iwtomicsrdata="${rdata}" + iwtomicstests="${testids}" + iwtomicsselectedfeatures="${featureids}" + + #set test_subset = '"\\|"'.join( [ str( $e.test ) for $e in $scalesection.thresholdontestscale ] ) + test_subset="${test_subset}" + #set feature_subset = '"\\|"'.join( [ str( $e.feature ) for $e in $scalesection.thresholdontestscale ] ) + feature_subset="${feature_subset}" + #set scale_subset = '","'.join( [ str( $e.scale ) for $e in $scalesection.thresholdontestscale ] ) + scale_subset="c(${scale_subset})" + + testalpha="${plotres.alpha}" + average="${plotres.average}" + size="${plotres.size}" + plottype="'${plotres.conditionalplottype.plottype}'" + #if $plotres.conditionalplottype.plottype == 'boxplot': + #set probs = '","'.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] ) + #if $probs != "": + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})" + #else: + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})" + #end if + #end if + + groupby="'${plotsum.conditionalgroupby.groupby}'" + #if $plotsum.conditionalgroupby.groupby == "test": + summaryalpha="${plotsum.conditionalgroupby.testalphaplot}" + only_significant="${plotsum.conditionalgroupby.testonlysig}" + #elif $plotsum.conditionalgroupby.groupby == "feature": + summaryalpha="${plotsum.conditionalgroupby.featurealphaplot}" + only_significant="${plotsum.conditionalgroupby.featureonlysig}" + #end if + + >& /dev/null +]]> + <!-- + to print the stack add the following line at the end of the command and enable the corresponding entry in output + 2> "${stackerr}" + --> + </command> + + <inputs> + <help> + ... + </help> + + <!-- RData --> + <param format="rdata" name="rdata" type="data" label="Select IWTomicsData object with test results" help="File created by 'IWTomics Test and Plot'." /> + <!-- test IDs --> + <param format="tabular" name="testids" type="data" label="Select test IDs" help="File created by 'IWTomics Test and Plot'." /> + <!-- feature IDs --> + <param format="tabular" name="featureids" type="data" label="Select feature IDs" help="File created by 'IWTomics Test and Plot'." /> + + <!-- repeat threshold on test scale --> + <section name="scalesection" title="Select scale for Interval-Wise Testing and plot" expanded="True" help="Maximum interval length for the p-value adjustment. If 0 the maximum possible scale is used."> + <repeat name="thresholdontestscale" title="Threshold on test scale" min="1"> + <param name="test" type="data_column" data_ref="testids" numerical="False" label="Test ID" multiple="True" use_header_names="True" /> + <param name="feature" type="data_column" data_ref="featureids" numerical="False" label="Feature ID" multiple="True" use_header_names="True" /> + <param name="scale" type="integer" value="0" min="0" label="Scale" /> + </repeat> + </section> + + <!-- plot IWTomics results --> + <section name="plotres" title="Plot IWTomics test results" expanded="True"> + <!-- alpha --> + <param name="alpha" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <!-- average --> + <param name="average" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot the mean curves" /> + <!-- average --> + <param name="size" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot sample size in each position" /> + <!-- conditional plot type --> + <conditional name="conditionalplottype"> + <!-- plot type --> + <param name="plottype" type="select" label="Plot type"> + <option value="boxplot">Pointwise quantile curves (boxplot)</option> + <option value="curves">Curves (aligned)</option> + </param> + <!-- conditional choice: plottype=boxplot --> + <when value="boxplot"> + <section name="probabilitiessection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantile curves to be drawn."> + <param name="prob0" size="3" type="float" value="0.25" min="0.0" max="1.0" label="Probability" /> + <param name="prob1" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + <param name="prob2" size="3" type="float" value="0.75" min="0.0" max="1.0" label="Probability" /> + <repeat name="probabilities" title="Probabilities"> + <param name="prob" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + </repeat> + </section> + </when> + </conditional> + </section> + + <!-- summary plot --> + <section name="plotsum" title="Summary plot" expanded="True"> + <!-- conditional group by --> + <conditional name="conditionalgroupby"> + <!-- group by --> + <param name="groupby" type="select" label="Group by" help="How tests should be grouped."> + <option value="none">No plot</option> + <option value="test">Group by test</option> + <option value="feature">Group by feature</option> + </param> + <when value="test"> + <param name="testalphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <param name="testonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> + </when> + <when value="feature"> + <param name="featurealphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <param name="featureonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> + </when> + </conditional> + </section> + </inputs> + + <outputs> + <!--<data format="txt" name="stackerr" label="iwtomics.testandplot.stackerr.txt" from_work_dir="iwtomics.testandplot.stackerr.txt" />--> + <data format="txt" name="adjustedpvalue" label="${tool.name} on ${on_string}: Adjusted p-value" from_work_dir="iwtomics.testandplot.adjustedpvalue.txt" /> + <data format="pdf" name="iwtomicsrespdf" label="${tool.name} on ${on_string}: Plotted Test Results" from_work_dir="iwtomics.testandplot.iwtomicstestresults.pdf" /> + <data format="pdf" name="iwtomicssumpdf" label="${tool.name} on ${on_string}: Summary Plot" from_work_dir="iwtomics.testandplot.summaryplot.pdf" /> + </outputs> + + <help> +This tool allows to select the scale for the Interval-Wise Testing results. In particular, it returns the p-value curves +for the different tests performed at the selected scale, and it creates a graphical representation of the +Interval-Wise Testing results and a summary plot (optional) at the selected scale. + +----- + +**Input files** + +RData file with the IWTomicsData object with test results, tabular files with test IDs and feature IDs. +These files are created by the tool *IWTomics Test and Plot*. + +----- + +**Output** + +The tool returns: + +1. TXT file with an adjusted p-value curve for every test performed at the selected scale; +2. PDF file with the plotted test results; +3. PDF file with the summary plot. + +----- + +.. class:: infomark + +**Notes** + +This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). + +It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects. +The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). + +.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf + </help> + + <citations> + <citation type="bibtex"> + @ARTICLE{ + iwt-functional-data, + author = {A Pini and S. Vantini}, + title = {Interval-Wise Testing for functional data}, + journal = {Journal of Nonparametric Statistics}, + year = {2017}, + volume = {29}, + number = {2}, + pages = {407-424} + } + </citation> + <citation type="bibtex"> + @MANUAL{ + iwtomics, + author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, + title = {IWTomics: Interval-Wise Testing for Omics Data}, + note = {R package version 0.99.12}, + year = {2017} + } + </citation> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/testandplot.R Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,99 @@ +if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { + args=commandArgs(TRUE) + + # get args names and values + args_values=strsplit(args,'=') + args_names=unlist(lapply(args_values,function(arg) arg[1])) + names(args_values)=args_names + args_values=lapply(args_values,function(arg) arg[2]) + # read filenames + adjustedpvaluematrix=args_values$adjustedpvaluematrix + iwtomicsrespdf=args_values$iwtomicsrespdf + iwtomicssumpdf=args_values$iwtomicssumpdf + regionids=args_values$regionids + featureids=args_values$featureids + rdatafile=args_values$rdatafile + iwtomicsrdata=args_values$iwtomicsrdata + iwtomicstests=args_values$iwtomicstests + iwtomicsselectedfeatures=args_values$iwtomicsselectedfeatures + # read parameters (from region1 on) + i_region1=which(args_names=='region1') + for(i in i_region1:length(args)){ + eval(parse(text=args[[i]])) + } + + # load RData + load(rdatafile) + # read regionids and featureids + regionids=as.character(read.delim(regionids,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + featureids=as.character(read.delim(featureids,header=FALSE,sep='\t',stringsAsFactors=FALSE)) + # retrieve region1, region2 and features_subset ids and check they are in the RData + id_region1=regionids[region1] + id_region2=regionids[region2] + id_features_subset=featureids[features_subset] + if(length(setdiff(c(id_region1,id_region2),idRegions(regionsFeatures)))!=0){ + quit(save="no", status=10) + stop('Wrong region ids') + } + if(length(setdiff(id_features_subset,idFeatures(regionsFeatures)))!=0){ + quit(save="no", status=20) + stop('Wrong feature ids') + } + if(sum(duplicated(paste0(id_region1,id_region2)))){ + quit(save="no", status=30) + stop('Same test repeated multiple times.') + } + + # perform test + tryCatch({ + # fix repeated probs + if(statistics=='quantile'){ + # fix repeated probs + testprobs=sort(unique(testprobs)) + }else{ + testprobs=0.5 + } + regionsFeatures_test=IWTomicsTest(regionsFeatures,id_region1,id_region2,id_features_subset, + statistics=statistics,probs=testprobs,B=B) + # create adjustedvaluematrix output + for(test in seq_along(id_region1)){ + for(id_feature in id_features_subset){ + write(paste0('Test: ',id_region1[test],' vs ',id_region2[test],', on feature ',id_feature), + file=adjustedpvaluematrix,append=TRUE,sep='\t') + pval=regionsFeatures_test@test$result[[test]][[id_feature]]$adjusted_pval_matrix + row.names(pval)=paste('Scale',rev(seq_len(nrow(pval)))) + write.table(pval,file=adjustedpvaluematrix,append=TRUE,sep='\t',quote=FALSE,row.names=TRUE,col.names=FALSE) + write('',file=adjustedpvaluematrix,append=TRUE,sep='\t') + } + } + }, error = function(err) { + quit(save="no", status=40) #error testing + stop(err) + }) + + # plot test results + pdf(iwtomicsrespdf,width=5,height=7) + if(plottype=='boxplot'){ + # fix repeated probs + probs=sort(unique(probs)) + }else{ + probs=c(0.25,0.5,0.75) + } + plotTest(regionsFeatures_test,alpha=testalpha,type=plottype,probs=probs,average=average,size=size,ask=FALSE) + dev.off() + + # plot summary results + if(groupby!='none'){ + pdf(iwtomicssumpdf,width=15,height=10) + plotSummary(regionsFeatures_test,alpha=summaryalpha,only_significant=only_significant,groupby=groupby,ask=FALSE,append=TRUE) + dev.off() + } + + # create output + write.table(as.data.frame(t(paste(id_region1,'vs',id_region2))),file=iwtomicstests,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + write.table(as.data.frame(t(idFeatures(regionsFeatures_test))),file=iwtomicsselectedfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) + save(regionsFeatures_test,file=iwtomicsrdata) +}else{ + quit(save="no", status=255) + stop("Missing IWTomics package") +} \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/iwtomics/testandplot.xml Wed May 31 11:06:00 2017 -0400 @@ -0,0 +1,239 @@ +<tool id="testandplot" name="IWTomics Test" version="@VERSION@.0"> + <description>and Plot</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <stdio> + <exit_code range="-1" source="both" level="fatal" description="Missing IWTomics package. Please be sure to have it installed before using this tool." /> + <exit_code range="10" source="both" level="fatal" description="Wrong region ids." /> + <exit_code range="20" source="both" level="fatal" description="Wrong feature ids." /> + <exit_code range="30" source="both" level="fatal" description="Same test repeated multiple times." /> + <exit_code range="40" source="both" level="fatal" description="Testing error." /> + <regex match="Error" source="both" level="fatal" description="An error has occurred. Please try again." /> + </stdio> + + <command> +<![CDATA[ + Rscript $__tool_directory__/testandplot.R adjustedpvaluematrix="${adjustedpvaluematrix}" iwtomicsrespdf="${iwtomicsrespdf}" iwtomicssumpdf="${iwtomicssumpdf}" iwtomicsrdata="${iwtomicsrdata}" iwtomicstests="${iwtomicstests}" iwtomicsselectedfeatures="${iwtomicsselectedfeatures}" + regionids="${regionids}" + featureids="${featureids}" + rdatafile="${rdata}" + + #set region1 = '","'.join( [ str( $r.region0 ) for $r in $regionssection.regions ] ) + #set region2 = '","'.join( [ str( $r.region1 ) for $r in $regionssection.regions ] ) + region1="c(${region1})" + region2="c(${region2})" + + features_subset="c(${featureslist})" + + statistics="'${conditionalstatistics.statistics}'" + #if $conditionalstatistics.statistics == "quantile": + #set probabilities = '","'.join( [ str( $p.qprob ) for $p in $conditionalstatistics.quantilesection.qprobabilities ] ) + testprobs="c(${probabilities})" + #end if + + B="${permutations}" + + testalpha="${plotres.alpha}" + average="${plotres.average}" + size="${plotres.size}" + plottype="'${plotres.conditionalplottype.plottype}'" + #if $plotres.conditionalplottype.plottype == 'boxplot': + #set probs = '","'.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] ) + #if $probs != "": + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})" + #else: + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})" + #end if + #end if + + groupby="'${plotsum.conditionalgroupby.groupby}'" + #if $plotsum.conditionalgroupby.groupby == "test": + summaryalpha="${plotsum.conditionalgroupby.testalphaplot}" + only_significant="${plotsum.conditionalgroupby.testonlysig}" + #elif $plotsum.conditionalgroupby.groupby == "feature": + summaryalpha="${plotsum.conditionalgroupby.featurealphaplot}" + only_significant="${plotsum.conditionalgroupby.featureonlysig}" + #end if + + >& /dev/null +]]> + <!-- + to print the stack add the following line at the end of the command and enable the corresponding entry in output + 2> "${stackerr}" + --> + </command> + + <inputs> + <!-- RData --> + <param format="rdata" name="rdata" type="data" label="Select IWTomicsData object" help="File created by 'IWTomics Load Smooth and Plot'." /> + <!-- region IDs --> + <param format="tabular" name="regionids" type="data" label="Select region dataset IDs" help="File created by 'IWTomics Load Smooth and Plot'." /> + <!-- feature IDs --> + <param format="tabular" name="featureids" type="data" label="Select feature IDs" help="File created by 'IWTomics Load Smooth and Plot'." /> + + <!-- repeat region ids --> + <section name="regionssection" title="Select regions for Interval-Wise Testing" expanded="True" help="IDs of the region datasets to be tested."> + <repeat name="regions" title="Two-sample test" min="1"> + <param name="region0" type="data_column" data_ref="regionids" numerical="False" label="Region 1" multiple="False" use_header_names="True" /> + <param name="region1" type="data_column" data_ref="regionids" numerical="False" label="Region 2" multiple="False" use_header_names="True" /> + </repeat> + </section> + + <!-- feature ids list --> + <param name="featureslist" type="data_column" data_ref="featureids" numerical="False" label="Select features" multiple="True" use_header_names="True" help="IDs of the features to be tested." /> + + <!-- conditional statistics --> + <conditional name="conditionalstatistics"> + <!-- statistics --> + <param name="statistics" type="select" label="Test statistics"> + <option value="mean">Mean difference</option> + <option value="median">Median difference</option> + <option value="variance">Variance ratio</option> + <option value="quantile">Quantile difference(s)</option> + </param> + <!-- conditional choice: statistics=quantile --> + <when value="quantile"> + <section name="quantilesection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantiles in test statistics."> + <repeat name="qprobabilities" title="Probabilities" min="1"> + <param name="qprob" size="4" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + </repeat> + </section> + </when> + </conditional> + + <!-- permutations --> + <param name="permutations" type="integer" value="1000" min="1" label="Number of permutations" /> + + <!-- plot IWTomics results --> + <section name="plotres" title="Plot IWTomics test results" expanded="True"> + <!-- alpha --> + <param name="alpha" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <!-- average --> + <param name="average" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot the mean curves" /> + <!-- average --> + <param name="size" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot sample size in each position" /> + <!-- conditional plot type --> + <conditional name="conditionalplottype"> + <!-- plot type --> + <param name="plottype" type="select" label="Plot type"> + <option value="boxplot">Pointwise quantile curves (boxplot)</option> + <option value="curves">Curves (aligned)</option> + </param> + <!-- conditional choice: plottype=boxplot --> + <when value="boxplot"> + <section name="probabilitiessection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantile curves to be drawn."> + <param name="prob0" size="3" type="float" value="0.25" min="0.0" max="1.0" label="Probability" /> + <param name="prob1" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + <param name="prob2" size="3" type="float" value="0.75" min="0.0" max="1.0" label="Probability" /> + <repeat name="probabilities" title="Probabilities"> + <param name="prob" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + </repeat> + </section> + </when> + </conditional> + </section> + + <!-- summary plot --> + <section name="plotsum" title="Summary plot" expanded="True"> + <!-- conditional group by --> + <conditional name="conditionalgroupby"> + <!-- group by --> + <param name="groupby" type="select" label="Group by" help="How tests should be grouped."> + <option value="none">No plot</option> + <option value="test">Group by test</option> + <option value="feature">Group by feature</option> + </param> + <when value="test"> + <param name="testalphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <param name="testonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> + </when> + <when value="feature"> + <param name="featurealphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <param name="featureonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> + </when> + </conditional> + </section> + </inputs> + + <outputs> + <!--<data format="txt" name="stackerr" label="iwtomics.testandplot.stackerr.txt" from_work_dir="iwtomics.testandplot.stackerr.txt" />--> + <data format="txt" name="adjustedpvaluematrix" label="${tool.name} on ${on_string}: Adjusted p-value Matrix" from_work_dir="iwtomics.testandplot.adjustedpvalue.matrix.txt" /> + <data format="pdf" name="iwtomicsrespdf" label="${tool.name} on ${on_string}: Plotted Test Results" from_work_dir="iwtomics.testandplot.iwtomicstestresults.pdf" /> + <data format="pdf" name="iwtomicssumpdf" label="${tool.name} on ${on_string}: Summary Plot" from_work_dir="iwtomics.testandplot.summaryplot.pdf" /> + <data format="rdata" name="iwtomicsrdata" label="${tool.name} on ${on_string}: IWTomicsData Object with Test Results" from_work_dir="iwtomics.testandplot.RData" /> + <data format="tabular" name="iwtomicstests" label="${tool.name} on ${on_string}: Test IDs" from_work_dir="iwtomics.testandplot.tests.txt" /> + <data format="tabular" name="iwtomicsselectedfeatures" label="${tool.name} on ${on_string}: Feature IDs" from_work_dir="iwtomics.testandplot.selectedfeatures.txt" /> + </outputs> + + <help> +This tool statistically evaluates differences in genomic features between groups of regions along the genome. +In particular, it implements the Interval-Wise Testing for omics data, an extended version of the Interval-Wise +Testing for functional data presented in Pini and Vantini (2017). It allows to perform multiple two sample permutation +tests between pairs of region datasets, on several features. It returns the adjusted p-value curves for every test +and all possible scales. Moreover, it creates a graphical representation of the Interval-Wise Testing results +and a summary plot (optional) with p-values at the maximum scale. The tool *IWTomics Plot with Threshold on Test Scale* +permits to select the scale to be used in the plots. + +----- + +**Input files** + +RData file with the IWTomicsData object, tabular files with region dataset IDs and feature IDs. +These files are created by the tool *IWTomics Load Smooth and Plot*. + +----- + +**Output** + +The tool returns: + +1. TXT file with an adjusted p-value matrix for every test performed. Each matrix contains a p-value curve (row) for every scale considered in the test; +2. PDF file with the plotted test results; +3. PDF file with the summary plot; +4. RData with the IWTomicsData object with the test results; +5. Test identifiers; +6. Feature identifiers. + +4-6 can be used as input of the tool *IWTomics Plot with Threshold on Test Scale* + +----- + +.. class:: infomark + +**Notes** + +This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). + +It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects. +The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). + +.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf + </help> + + <citations> + <citation type="bibtex"> + @ARTICLE{ + iwt-functional-data, + author = {A Pini and S. Vantini}, + title = {Interval-Wise Testing for functional data}, + journal = {Journal of Nonparametric Statistics}, + year = {2017}, + volume = {29}, + number = {2}, + pages = {407-424} + } + </citation> + <citation type="bibtex"> + @MANUAL{ + iwtomics, + author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, + title = {IWTomics: Interval-Wise Testing for Omics Data}, + note = {R package version 0.99.12}, + year = {2017} + } + </citation> + </citations> + +</tool>
--- a/loadandplot.R Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,146 +0,0 @@ -if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { - require(tools,quietly = FALSE) - args=commandArgs(TRUE) - - for (i in seq_along(args)) { - message(args[[i]]) - } - - # get args names and values - args_values=strsplit(args,'=') - args_names=unlist(lapply(args_values,function(arg) arg[1])) - names(args_values)=args_names - args_values=lapply(args_values,function(arg) arg[2]) - # read filenames - outrdata=args_values$outrdata - outregions=args_values$outregions - outfeatures=args_values$outfeatures - outpdf=args_values$outpdf - regionspaths=unlist(strsplit(args_values$regionspaths,'\\|')) - if("regionsheaderfile" %in% args_names){ - # the file regionsheaderfile must contain as first column the (unique) regionsfilenames, - # as second column the corresponding ids and as third column the names - tryCatch({ - regionsheader=read.delim(args_values$regionsheaderfile,header=FALSE,stringsAsFactors=FALSE,row.names=1,sep="\t") - regionsfilenames=unlist(strsplit(args_values$regionsfilenames,'\\|')) - if(length(setdiff(regionsfilenames,row.names(regionsheader)))) { - quit(save="no", status=11) - stop('Not all regionsfilenames are present in the first column of regionsheader.') - } - id_regions=regionsheader[regionsfilenames,1] - name_regions=regionsheader[regionsfilenames,2] - }, error = function(err) { - quit(save="no", status=10) #error on header file - stop(err) - }) - }else{ - eval(parse(text=args[[which(args_names=='regionsgalaxyids')]])) - id_regions=paste0('data_',regionsgalaxyids) - name_regions=paste0('data_',regionsgalaxyids) - } - featurespaths=unlist(strsplit(args_values$featurespaths,'\\|')) - if("featuresheaderfile" %in% args_names){ - # the file featuresheaderfile must contain as first column the (unique) featuresfilenames, - # as second column the corresponding ids and as third column the names - tryCatch({ - featuresheader=read.delim(args_values$featuresheaderfile,header=FALSE,stringsAsFactors=FALSE,row.names=1,sep="\t") - featuresfilenames=unlist(strsplit(args_values$featuresfilenames,'\\|')) - if(length(setdiff(featuresfilenames,row.names(featuresheader)))) { - quit(save="no", status=21) - stop('Not all featuresfilenames are present in the first column of featuresheader.') - } - id_features=featuresheader[featuresfilenames,1] - name_features=featuresheader[featuresfilenames,2] - }, error = function(err) { - quit(save="no", status=20) #error on header file - stop(err) - }) - }else{ - eval(parse(text=args[[which(args_names=='featuresgalaxyids')]])) - id_features=paste0('data_',featuresgalaxyids) - name_features=paste0('data_',featuresgalaxyids) - } - # read parameters (from smoothing on) - i_smoothing=which(args_names=='smoothing') - for(i in i_smoothing:length(args)){ - eval(parse(text=args[[i]])) - } - - # load data - tryCatch({ - regionsFeatures=IWTomicsData(regionspaths,featurespaths,alignment, - id_regions,name_regions,id_features,name_features,start.are.0based=start.are.0based) - }, error = function(err) { - if(grepl('invalid format',err$message)){ - quit(save="no", status=31) # error, not enough columns in input file - }else if(grepl('duplicated regions',err$message)){ - quit(save="no", status=32) # error, duplicated regions in region file - }else if(grepl('duplicated windows',err$message)){ - quit(save="no", status=33) # error, duplicated windows in feature file - }else if(grepl('overlapping windows',err$message)){ - quit(save="no", status=34) # error, overlapping windows in feature file - }else if(grepl('not all regions in datasets',err$message)){ - quit(save="no", status=35) # error, windows in feature files do not cover all regions in region files - }else if(grepl('ifferent size windows',err$message)){ - quit(save="no", status=36) # error, all windows in a feature files must have the same size - } - #error loading data - - stop(err) - - quit(save="no", status=30) - - }) - - # smooth data - if(smoothing!='no'){ - tryCatch({ - if(smoothing=='locpoly'){ - dist_knots=10 - }else if(smoothing=='kernel'){ - degree=3 - dist_knots=10 - }else if(smoothing=='splines'){ - bandwidth=5 - } - if(alignment=='scale'){ - if(scale==0){ - regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, - bandwidth=bandwidth,degree=degree,dist_knots=dist_knots) - }else{ - regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, - bandwidth=bandwidth,degree=degree,dist_knots=dist_knots,scale_grid=scale) - } - }else{ - regionsFeatures=smooth(regionsFeatures,type=smoothing,fill_gaps=fill_gaps, - bandwidth=bandwidth,degree=degree,dist_knots=dist_knots) - } - }, error = function(err) { - quit(save="no", status=40) #error on smoothing - stop(err) - }) - } - - # plot data - pdf(outpdf,width=10,height=8) - if(plottype=='boxplot'){ - # fix repeated probs - probs=sort(unique(probs)) - }else{ - probs=c(0.25,0.5,0.75) - } - plot(regionsFeatures,type=plottype,probs=probs,average=average,size=size,ask=FALSE) - dev.off() - - # create output - #write.table(cbind(unlist(strsplit(args_values$regionsfilenames,'\\|')),idRegions(regionsFeatures),nameRegions(regionsFeatures)), - #file=outregions,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) - write.table(as.data.frame(t(idRegions(regionsFeatures))),file=outregions,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) - #write.table(cbind(unlist(strsplit(args_values$featuresfilenames,'\\|')),idFeatures(regionsFeatures),nameFeatures(regionsFeatures)), - #file=outfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) - write.table(as.data.frame(t(idFeatures(regionsFeatures))),file=outfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) - save(regionsFeatures,file=outrdata) -}else{ - quit(save="no", status=255) - stop("Missing IWTomics package") -} \ No newline at end of file
--- a/loadandplot.xml Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,321 +0,0 @@ -<tool id="loadandplot" name="IWTomics Load" version="0.1.0"> - <description>Smooth and Plot</description> - <command interpreter="Rscript"> -<![CDATA[ - loadandplot.R outrdata="${outrdata}" outregions="${outregions}" outfeatures="${outfeatures}" outpdf="${outpdf}" - - #set region_paths = '"\\|"'.join( [ str( $r ) for $r in $regions ] ) - regionspaths="${region_paths}" - #set region_galaxyids = '","'.join( [ str( $r.hid ) for $r in $regions ] ) - regionsgalaxyids="c(${region_galaxyids})" - #set region_names = '"\\|"'.join( [ str( $r.name ) for $r in $regions ] ) - regionsfilenames="${region_names}" - #set region_header = '"\\|"'.join( [ str( $rh.regionsheader ) for $rh in $regionsheaderrepeat ] ) - #if $region_header != "": - regionsheaderfile="${region_header}" - #end if - - #set feature_paths = '"\\|"'.join( [ str( $f ) for $f in $features ] ) - featurespaths="${feature_paths}" - #set feature_galaxyids = '","'.join( [ str( $f.hid ) for $f in $features ] ) - featuresgalaxyids="c(${feature_galaxyids})" - #set feature_names = '"\\|"'.join( [ str( $f.name ) for $f in $features ] ) - featuresfilenames="${feature_names}" - #set feature_header = '"\\|"'.join( [ str( $fh.featuresheader ) for $fh in $featuresheaderrepeat ] ) - #if $feature_header != "": - featuresheaderfile="${feature_header}" - #end if - - smoothing="'${conditionaltype.smoothing}'" - start.are.0based="${zerobased}" - #if $conditionaltype.smoothing == 'no': - alignment="'${conditionaltype.alignment}'" - #elif $conditionaltype.smoothing == 'locpoly': - alignment="'${conditionaltype.locpolyconditionalscale.alignment}'" - #if $conditionaltype.locpolyconditionalscale.alignment == 'scale': - scale="${conditionaltype.locpolyconditionalscale.scalegrid}" - #end if - bandwidth="${conditionaltype.locpolybandwidth}" - degree="${conditionaltype.locpolydegree}" - fill_gaps="${conditionaltype.fillgaps}" - #elif $conditionaltype.smoothing == 'kernel': - alignment="'${conditionaltype.kernelconditionalscale.alignment}'" - #if $conditionaltype.kernelconditionalscale.alignment == 'scale': - scale="${conditionaltype.kernelconditionalscale.scalegrid}" - #end if - bandwidth="${conditionaltype.kernelbandwidth}" - fill_gaps="${conditionaltype.fillgaps}" - #elif $conditionaltype.smoothing == 'splines': - alignment="'${conditionaltype.splinesconditionalscale.alignment}'" - #if $conditionaltype.splinesconditionalscale.alignment == 'scale': - scale="${conditionaltype.splinesconditionalscale.scalegrid}" - #end if - degree="${conditionaltype.splinesdegree}" - dist_knots="${conditionaltype.splinesdistknots}" - fill_gaps="${conditionaltype.fillgaps}" - #end if - - average="${plotres.average}" - size="${plotres.size}" - plottype="'${plotres.conditionalplottype.plottype}'" - #if $plotres.conditionalplottype.plottype == 'boxplot': - #set probs = '","'.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] ) - #if $probs != "": - probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})" - #else: - probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})" - #end if - #end if - - >& /dev/null -]]> - <!-- - to print the stack add the following line at the end of the command and enable the corresponding entry in output - 2> "${stackerr}" - --> - </command> - - <inputs> - <!-- regions --> - <param format="tabular" name="regions" type="data" label="Select region files" multiple="true" help="" /> - <repeat name="regionsheaderrepeat" title="Header file for regions (optional)" min="0" max="1" help="Tabular file. Column 1: names of the region files; Column 2: unique identifiers of the region datasets (spaces and special characters are not allowed); Column 3: name of the region datasets to be used in the output."> - <param format="tabular" name="regionsheader" type="data" label="Select header file" /> - </repeat> - - <!-- features --> - <param format="tabular" name="features" type="data" label="Select feature files" multiple="true" help="" /> - <repeat name="featuresheaderrepeat" title="Header file for features (optional)" min="0" max="1" help="Tabular file. Column 1: names of the feature files; Column 2: unique identifiers of the features (spaces and special characters are not allowed); Column 3: name of the features to be used in the output."> - <param format="tabular" name="featuresheader" type="data" label="Select header file" /> - </repeat> - - <!-- zero-base one-base --> - <param name="zerobased" type="select" label="Start positions are:" multiple="false" help=""> - <option value="TRUE">0-based</option> - <option value="FALSE">1-based</option> - </param> - - <!-- conditional smoothing --> - <conditional name="conditionaltype"> - <!-- smoothing --> - <param name="smoothing" type="select" label="Smoothing" help="Type of smoothing to be applied to the feature curves."> - <option value="no">No</option> - <option value="locpoly">Local Polynomials</option> - <option value="kernel">Gaussian Kernel</option> - <option value="splines">B-Splines</option> - </param> - <!-- conditional choice: smoothing=no --> - <when value="no"> - <param name="alignment" type="select" label="Region alignment" help=""> - <option value="center">Center - Alignment on the central position</option> - <option value="left">Left - Alignment on the starting position</option> - <option value="right">Right - Alignment on the ending position</option> - </param> - </when> - <!-- conditional choice: smoothing=locpoly --> - <when value="locpoly"> - <!-- conditional region alignment --> - <conditional name="locpolyconditionalscale"> - <param name="alignment" type="select" label="Region alignment" help=""> - <option value="center">Center - Alignment on the central position</option> - <option value="left">Left - Alignment on the starting position</option> - <option value="right">Right - Alignment on the ending position</option> - <option value="scale">Scale - Scaling all regions to the same length</option> - </param> - <when value="scale"> - <param name="scalegrid" type="integer" value="0" min="0" label="Number of grid points" help="Number of equally-spaced grid points over which the smoothed curves are evaluated." /> - </when> - </conditional> - - <param name="locpolybandwidth" type="integer" value="5" min="2" label="Bandwidth for smoothing" help="" /> - <param name="locpolydegree" type="integer" value="3" min="0" label="Degree of local polynomial" help="" /> - - <!-- fill gaps --> - <param name="fillgaps" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Use smoothing to fill gaps (NA measurements)" help="" /> - </when> - <!-- conditional choice: smoothing=kernel --> - <when value="kernel"> - <!-- conditional region alignment --> - <conditional name="kernelconditionalscale"> - <param name="alignment" type="select" label="Region alignment" help=""> - <option value="center">Center - Alignment on the central position</option> - <option value="left">Left - Alignment on the starting position</option> - <option value="right">Right - Alignment on the ending position</option> - <option value="scale">Scale - Scaling all regions to the same length</option> - </param> - <when value="scale"> - <param name="scalegrid" type="integer" value="0" min="0" label="Number of grid points" help="Number of equally-spaced grid points over which the smoothed curves are evaluated." /> - </when> - </conditional> - - <param name="kernelbandwidth" type="integer" value="5" min="2" label="Bandwidth for smoothing" help="" /> - - <!-- fill gaps --> - <param name="fillgaps" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Use smoothing to fill gaps (NA measurements)" help="" /> - </when> - <!-- conditional choice: smoothing=splines --> - <when value="splines"> - <!-- conditional region alignment --> - <conditional name="splinesconditionalscale"> - <param name="alignment" type="select" label="Region alignment" help=""> - <option value="center">Center - Alignment on the central position</option> - <option value="left">Left - Alignment on the starting position</option> - <option value="right">Right - Alignment on the ending position</option> - <option value="scale">Scale - Scaling all regions to the same length</option> - </param> - <when value="scale"> - <param name="scalegrid" type="integer" value="0" min="0" label="Number of grid points" help="Number of equally-spaced grid points over which the smoothed curves are evaluated." /> - </when> - </conditional> - - <param name="splinesdegree" type="integer" value="3" min="0" label="Degree of B-splines" help="" /> - <param name="splinesdistknots" type="integer" value="10" min="2" label="Distance between nodes (approximate)" help="" /> - - <!-- fill gaps --> - <param name="fillgaps" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Use smoothing to fill gaps (NA measurements)" help="" /> - </when> - </conditional> - - <!-- plot IWTomics results --> - <section name="plotres" title="Plot data" expanded="True"> - <!-- average --> - <param name="average" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot the mean curves" help="" /> - <!-- sample size in each position --> - <param name="size" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot sample size in each position" help="" /> - <!-- conditional plot type --> - <conditional name="conditionalplottype"> - <!-- plot type --> - <param name="plottype" type="select" label="Plot type" help=""> - <option value="boxplot">Pointwise quantile curves (boxplot)</option> - <option value="curves">Curves (aligned)</option> - </param> - <!-- conditional choice: plottype=boxplot --> - <when value="boxplot"> - <section name="probabilitiessection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantile curves to be drawn."> - <param name="prob0" size="3" type="float" value="0.25" min="0.0" max="1.0" label="Probability" /> - <param name="prob1" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> - <param name="prob2" size="3" type="float" value="0.75" min="0.0" max="1.0" label="Probability" /> - <repeat name="probabilities" title="Probabilities"> - <param name="prob" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> - </repeat> - </section> - </when> - </conditional> - </section> - </inputs> - - <outputs> - <!--<data format="txt" name="stackerr" label="iwtomics.loadandplot.stackerr.txt" from_work_dir="iwtomics.loadandplot.stackerr.txt" />--> - <data format="rdata" name="outrdata" label="${tool.name} on ${on_string}: IWTomicsData Object" from_work_dir="iwtomics.loadandplot.RData" /> - <data format="tabular" name="outregions" label="${tool.name} on ${on_string}: Region Dataset IDs" from_work_dir="iwtomics.loadandplot.regions.txt" /> - <data format="tabular" name="outfeatures" label="${tool.name} on ${on_string}: Feature IDs" from_work_dir="iwtomics.loadandplot.features.txt" /> - <data format="pdf" name="outpdf" label="${tool.name} on ${on_string}: Plotted Data" from_work_dir="iwtomics.loadandplot.pdf" /> - </outputs> - - <help> -This tool imports a collection of genomic region datasets, and associates to each region -multiple genomic feature measurements. It allows to align the regions in multiple ways -(center, left, right or scale alignment), to smooth the feature curves (possibly filling gaps in the -measurements) and to create a graphical representation of the feature measurements in each -region datasets (aligned curves or pointwise quantile curves). - ------ - -**Region datasets** - -Each region dataset can be provided as a BED or Tabular file with tab delimited columns chr -start end (extra columns present in the input file are ignored). Regions can be of different -length:: - - chr2 49960150 50060150 - chr2 55912445 56012445 - ... - ------ - -**Feature measurements** - -Feature measurements corresponding to all the regions can be provided as a BED or Tabular -file with tab delimited columns chr start end value:: - - chr2 49960150 49962150 0.9426 - chr2 49962150 49964150 0.7816 - ... - -Each feature must be measured in windows of a fixed size inside all the regions (missing -values must be indicated as NA). Another way to import feature measurements is from a -Tabular file with the first three columns chr start end corresponding to the different genomic -regions, followed on the same row by all the measurements in fixed-size windows:: - - chr2 49960150 50060150 0.9426 0.7816 0.8921 ... ... 1.2063 - chr2 55912445 56012445 0.8719 0.9975 1.1619 ... ... 0.9601 - ... - ------ - -**Output** - -The tool returns: - -1. RData with the IWTomicsData object, that stores the aligned genomic region datasets, and their associated feature measurements; -2. Region dataset identifiers; -3. Feature identifiers; -4. PDF file with the plotted data. - -1-3 can be used as input of the tool *IWTomics Test and Plot* - ------ - -.. class:: infomark - -**Notes** - -This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). - -It implements a simplified version of the methods *smooth* and *plot* for *IWTomicsData* objects. -The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). - -.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf - </help> - - <citations> - <citation type="bibtex"> - @ARTICLE{ - iwt-functional-data, - author = {Pini, Alessia and Vantini, Simone}, - title = {Interval-Wise Testing for functional data}, - journal = {Journal of Nonparametric Statistics}, - year = {2017}, - volume = {29}, - number = {2}, - pages = {407-424} - } - </citation> - <citation type="bibtex"> - @MANUAL{ - iwtomics, - author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, - title = {IWTomics: Interval-Wise Testing for Omics Data}, - note = {R package version 0.99.12}, - year = {2017} - } - </citation> - </citations> - - <stdio> - <exit_code range="255" source="both" level="fatal" description="Missing IWTomics package. Please be sure to have it installed before using this tool." /> - <exit_code range="10" source="both" level="fatal" description="An error has occurred reading the header file for regions. Please try again." /> - <exit_code range="11" source="both" level="fatal" description="Not all region files are present in the first column of header file for regions." /> - <exit_code range="20" source="both" level="fatal" description="An error has occurred reading the header file for features. Please try again." /> - <exit_code range="21" source="both" level="fatal" description="Not all feature files are present in the first column of header file for features." /> - <exit_code range="30" source="both" level="fatal" description="An error has occurred reading the data. Please try again." /> - <exit_code range="31" source="both" level="fatal" description="Not enough columns in input file." /> - <exit_code range="32" source="both" level="fatal" description="Duplicated regions in region file." /> - <exit_code range="33" source="both" level="fatal" description="Duplicated windows in feature file." /> - <exit_code range="34" source="both" level="fatal" description="Overlapping windows in feature file." /> - <exit_code range="35" source="both" level="fatal" description="Windows in feature files do not cover all regions in region files." /> - <exit_code range="36" source="both" level="fatal" description="All windows in a feature file must have the same size." /> - <exit_code range="40" source="both" level="fatal" description="An error has occurred smoothing the data. Please try again." /> - <regex match="Error" source="both" level="fatal" description="An error has occurred. Please try again." /> - </stdio> - -</tool>
--- a/plotwithscale.R Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,117 +0,0 @@ -if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { - args=commandArgs(TRUE) - - # get args names and values - args_values=strsplit(args,'=') - args_names=unlist(lapply(args_values,function(arg) arg[1])) - names(args_values)=args_names - args_values=lapply(args_values,function(arg) arg[2]) - # read filenames - adjustedpvalue=args_values$adjustedpvalue - iwtomicsrespdf=args_values$iwtomicsrespdf - iwtomicssumpdf=args_values$iwtomicssumpdf - iwtomicsrdata=args_values$iwtomicsrdata - iwtomicstests=args_values$iwtomicstests - iwtomicsselectedfeatures=args_values$iwtomicsselectedfeatures - test_subset=paste0('c(',strsplit(args_values$test_subset,'\\|')[[1]],')') - feature_subset=paste0('c(',strsplit(args_values$feature_subset,'\\|')[[1]],')') - # read parameters (from test_subset on) - i_scale_subset=which(args_names=='scale_subset') - for(i in i_scale_subset:length(args)){ - eval(parse(text=args[[i]])) - } - - # load RData - load(iwtomicsrdata) - # read testids and featureids and check them - unlisted=lapply(seq_along(test_subset), - function(i){ - test_subset_i=eval(parse(text=test_subset[i])) - feature_subset_i=eval(parse(text=feature_subset[i])) - test_subset_i=rep(test_subset_i,each=length(feature_subset_i)) - feature_subset_i=rep(feature_subset_i,length.out=length(test_subset_i)) - scale_subset_i=rep(scale_subset[i],length(test_subset_i)) - return(list(test_subset=test_subset_i,feature_subset=feature_subset_i,scale_subset=scale_subset_i)) - }) - test_subset=unlist(lapply(unlisted,function(l) l$test_subset)) - feature_subset=unlist(lapply(unlisted,function(l) l$feature_subset)) - scale_subset=unlist(lapply(unlisted,function(l) l$scale_subset)) - testids=as.character(read.delim(iwtomicstests,header=FALSE,sep='\t',stringsAsFactors=FALSE)) - featureids=as.character(read.delim(iwtomicsselectedfeatures,header=FALSE,sep='\t',stringsAsFactors=FALSE)) - id_features_subset=featureids[feature_subset] - if(sum(testids!=paste(testInput(regionsFeatures_test)$id_region1,'vs',testInput(regionsFeatures_test)$id_region2))){ - quit(save="no", status=10) - stop('Wrong test ids') - } - if(sum(featureids!=idFeatures(regionsFeatures_test))){ - quit(save="no", status=20) - stop('Wrong feature ids') - } - # retrieve test and features_subset ids - id_features_subset=featureids[feature_subset] - if(sum(duplicated(paste0(test_subset,id_features_subset)))){ - quit(save="no", status=30) - stop('Two scale thresholds selected for the same test and feature.') - } - # If scale_subset=0, do not change the threshold - default=(scale_subset==0) - scale_subset=scale_subset[!default] - test_subset=test_subset[!default] - id_features_subset=id_features_subset[!default] - - # get scale threshold - scale_threshold=lapply(regionsFeatures_test@test$result, - function(result) unlist(lapply(result,function(feature) feature$max_scale))) - for(i in seq_along(test_subset)){ - if(scale_threshold[[test_subset[i]]][id_features_subset[i]]<scale_subset[i]){ - quit(save="no", status=40) - stop('Scale threshold too high.') - } - scale_threshold[[test_subset[i]]][id_features_subset[i]]=scale_subset[i] - } - - # create adjustedvalue output - pval=adjusted_pval(regionsFeatures_test,scale_threshold=scale_threshold) - for(test in seq_along(pval)){ - for(id_feature in idFeatures(regionsFeatures_test)){ - write(paste0('Test: ',testids[test],', on feature ',id_feature), - file=adjustedpvalue,append=TRUE,sep='\t') - pval_i=as.data.frame(t(pval[[test]][[id_feature]])) - row.names(pval_i)=paste('Scale',scale_threshold[[test]][[id_feature]]) - write.table(pval_i,file=adjustedpvalue,append=TRUE,sep='\t',quote=FALSE,row.names=TRUE,col.names=FALSE) - write('',file=adjustedpvalue,append=TRUE,sep='\t') - } - } - - - # plot test results - pdf(iwtomicsrespdf,width=5,height=7) - if(plottype=='boxplot'){ - # fix repeated probs - probs=sort(unique(probs)) - }else{ - probs=c(0.25,0.5,0.75) - } - plotTest(regionsFeatures_test,alpha=testalpha,type=plottype,probs=probs,average=average,size=size,scale_threshold=scale_threshold,ask=FALSE) - dev.off() - - # plot summary results - if(groupby!='none'){ - tryCatch({ - pdf(iwtomicssumpdf,width=15,height=10) - plotSummary(regionsFeatures_test,alpha=summaryalpha,only_significant=only_significant,groupby=groupby,scale_threshold=scale_threshold,ask=FALSE,append=TRUE) - dev.off() - }, error = function(err) { - if (grepl('selected features with different resolution',err$message)) { - quit(save="no", status=50) #error: groupby 'test' but selected features with different resolution. - stop(err) - } - quit(save="no", status=60) #error - stop(err) - }) - } - -}else{ - quit(save="no", status=255) - stop("Missing IWTomics package") -} \ No newline at end of file
--- a/plotwithscale.xml Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,197 +0,0 @@ -<tool id="plotwithscale" name="IWTomics Plot with Threshold" version="0.1.0"> - <description>on Test Scale</description> - <command interpreter="Rscript"> -<![CDATA[ - plotwithscale.R adjustedpvalue="${adjustedpvalue}" iwtomicsrespdf="${iwtomicsrespdf}" iwtomicssumpdf="${iwtomicssumpdf}" - iwtomicsrdata="${rdata}" - iwtomicstests="${testids}" - iwtomicsselectedfeatures="${featureids}" - - #set test_subset = '"\\|"'.join( [ str( $e.test ) for $e in $scalesection.thresholdontestscale ] ) - test_subset="${test_subset}" - #set feature_subset = '"\\|"'.join( [ str( $e.feature ) for $e in $scalesection.thresholdontestscale ] ) - feature_subset="${feature_subset}" - #set scale_subset = '","'.join( [ str( $e.scale ) for $e in $scalesection.thresholdontestscale ] ) - scale_subset="c(${scale_subset})" - - testalpha="${plotres.alpha}" - average="${plotres.average}" - size="${plotres.size}" - plottype="'${plotres.conditionalplottype.plottype}'" - #if $plotres.conditionalplottype.plottype == 'boxplot': - #set probs = '","'.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] ) - #if $probs != "": - probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})" - #else: - probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})" - #end if - #end if - - groupby="'${plotsum.conditionalgroupby.groupby}'" - #if $plotsum.conditionalgroupby.groupby == "test": - summaryalpha="${plotsum.conditionalgroupby.testalphaplot}" - only_significant="${plotsum.conditionalgroupby.testonlysig}" - #elif $plotsum.conditionalgroupby.groupby == "feature": - summaryalpha="${plotsum.conditionalgroupby.featurealphaplot}" - only_significant="${plotsum.conditionalgroupby.featureonlysig}" - #end if - - >& /dev/null -]]> - <!-- - to print the stack add the following line at the end of the command and enable the corresponding entry in output - 2> "${stackerr}" - --> - </command> - - <inputs> - <help> - ... - </help> - - <!-- RData --> - <param format="rdata" name="rdata" type="data" label="Select IWTomicsData object with test results" help="File created by 'IWTomics Test and Plot'." /> - <!-- test IDs --> - <param format="tabular" name="testids" type="data" label="Select test IDs" help="File created by 'IWTomics Test and Plot'." /> - <!-- feature IDs --> - <param format="tabular" name="featureids" type="data" label="Select feature IDs" help="File created by 'IWTomics Test and Plot'." /> - - <!-- repeat threshold on test scale --> - <section name="scalesection" title="Select scale for Interval-Wise Testing and plot" expanded="True" help="Maximum interval length for the p-value adjustment. If 0 the maximum possible scale is used."> - <repeat name="thresholdontestscale" title="Threshold on test scale" min="1"> - <param name="test" type="data_column" data_ref="testids" numerical="False" label="Test ID" multiple="True" use_header_names="True" /> - <param name="feature" type="data_column" data_ref="featureids" numerical="False" label="Feature ID" multiple="True" use_header_names="True" /> - <param name="scale" type="integer" value="0" min="0" label="Scale" /> - </repeat> - </section> - - <!-- plot IWTomics results --> - <section name="plotres" title="Plot IWTomics test results" expanded="True"> - <!-- alpha --> - <param name="alpha" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> - <!-- average --> - <param name="average" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot the mean curves" /> - <!-- average --> - <param name="size" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot sample size in each position" /> - <!-- conditional plot type --> - <conditional name="conditionalplottype"> - <!-- plot type --> - <param name="plottype" type="select" label="Plot type"> - <option value="boxplot">Pointwise quantile curves (boxplot)</option> - <option value="curves">Curves (aligned)</option> - </param> - <!-- conditional choice: plottype=boxplot --> - <when value="boxplot"> - <section name="probabilitiessection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantile curves to be drawn."> - <param name="prob0" size="3" type="float" value="0.25" min="0.0" max="1.0" label="Probability" /> - <param name="prob1" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> - <param name="prob2" size="3" type="float" value="0.75" min="0.0" max="1.0" label="Probability" /> - <repeat name="probabilities" title="Probabilities"> - <param name="prob" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> - </repeat> - </section> - </when> - </conditional> - </section> - - <!-- summary plot --> - <section name="plotsum" title="Summary plot" expanded="True"> - <!-- conditional group by --> - <conditional name="conditionalgroupby"> - <!-- group by --> - <param name="groupby" type="select" label="Group by" help="How tests should be grouped."> - <option value="none">No plot</option> - <option value="test">Group by test</option> - <option value="feature">Group by feature</option> - </param> - <when value="test"> - <param name="testalphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> - <param name="testonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> - </when> - <when value="feature"> - <param name="featurealphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> - <param name="featureonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> - </when> - </conditional> - </section> - </inputs> - - <outputs> - <!--<data format="txt" name="stackerr" label="iwtomics.testandplot.stackerr.txt" from_work_dir="iwtomics.testandplot.stackerr.txt" />--> - <data format="txt" name="adjustedpvalue" label="${tool.name} on ${on_string}: Adjusted p-value" from_work_dir="iwtomics.testandplot.adjustedpvalue.txt" /> - <data format="pdf" name="iwtomicsrespdf" label="${tool.name} on ${on_string}: Plotted Test Results" from_work_dir="iwtomics.testandplot.iwtomicstestresults.pdf" /> - <data format="pdf" name="iwtomicssumpdf" label="${tool.name} on ${on_string}: Summary Plot" from_work_dir="iwtomics.testandplot.summaryplot.pdf" /> - </outputs> - - <help> -This tool allows to select the scale for the Interval-Wise Testing results. In particular, it returns the p-value curves -for the different tests performed at the selected scale, and it creates a graphical representation of the -Interval-Wise Testing results and a summary plot (optional) at the selected scale. - ------ - -**Input files** - -RData file with the IWTomicsData object with test results, tabular files with test IDs and feature IDs. -These files are created by the tool *IWTomics Test and Plot*. - ------ - -**Output** - -The tool returns: - -1. TXT file with an adjusted p-value curve for every test performed at the selected scale; -2. PDF file with the plotted test results; -3. PDF file with the summary plot. - ------ - -.. class:: infomark - -**Notes** - -This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). - -It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects. -The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). - -.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf - </help> - - <citations> - <citation type="bibtex"> - @ARTICLE{ - iwt-functional-data, - author = {A Pini and S. Vantini}, - title = {Interval-Wise Testing for functional data}, - journal = {Journal of Nonparametric Statistics}, - year = {2017}, - volume = {29}, - number = {2}, - pages = {407-424} - } - </citation> - <citation type="bibtex"> - @MANUAL{ - iwtomics, - author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, - title = {IWTomics: Interval-Wise Testing for Omics Data}, - note = {R package version 0.99.12}, - year = {2017} - } - </citation> - </citations> - - <stdio> - <exit_code range="-1" source="both" level="fatal" description="Missing IWTomics package. Please be sure to have it installed before using this tool." /> - <exit_code range="10" source="both" level="fatal" description="Wrong test ids." /> - <exit_code range="20" source="both" level="fatal" description="Wrong feature ids." /> - <exit_code range="30" source="both" level="fatal" description="Two scale thresholds selected for the same test and feature." /> - <exit_code range="40" source="both" level="fatal" description="Scale threshold too high." /> - <exit_code range="50" source="both" level="fatal" description="Group by 'test' but selected features with different resolution." /> - <exit_code range="60" source="both" level="fatal" description="Summary plot error. Please try again." /> - <regex match="Error" source="both" level="fatal" description="An error has occurred. Please try again." /> - </stdio> - -</tool>
--- a/testandplot.R Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,99 +0,0 @@ -if (require("IWTomics",character.only = TRUE,quietly = FALSE)) { - args=commandArgs(TRUE) - - # get args names and values - args_values=strsplit(args,'=') - args_names=unlist(lapply(args_values,function(arg) arg[1])) - names(args_values)=args_names - args_values=lapply(args_values,function(arg) arg[2]) - # read filenames - adjustedpvaluematrix=args_values$adjustedpvaluematrix - iwtomicsrespdf=args_values$iwtomicsrespdf - iwtomicssumpdf=args_values$iwtomicssumpdf - regionids=args_values$regionids - featureids=args_values$featureids - rdatafile=args_values$rdatafile - iwtomicsrdata=args_values$iwtomicsrdata - iwtomicstests=args_values$iwtomicstests - iwtomicsselectedfeatures=args_values$iwtomicsselectedfeatures - # read parameters (from region1 on) - i_region1=which(args_names=='region1') - for(i in i_region1:length(args)){ - eval(parse(text=args[[i]])) - } - - # load RData - load(rdatafile) - # read regionids and featureids - regionids=as.character(read.delim(regionids,header=FALSE,sep='\t',stringsAsFactors=FALSE)) - featureids=as.character(read.delim(featureids,header=FALSE,sep='\t',stringsAsFactors=FALSE)) - # retrieve region1, region2 and features_subset ids and check they are in the RData - id_region1=regionids[region1] - id_region2=regionids[region2] - id_features_subset=featureids[features_subset] - if(length(setdiff(c(id_region1,id_region2),idRegions(regionsFeatures)))!=0){ - quit(save="no", status=10) - stop('Wrong region ids') - } - if(length(setdiff(id_features_subset,idFeatures(regionsFeatures)))!=0){ - quit(save="no", status=20) - stop('Wrong feature ids') - } - if(sum(duplicated(paste0(id_region1,id_region2)))){ - quit(save="no", status=30) - stop('Same test repeated multiple times.') - } - - # perform test - tryCatch({ - # fix repeated probs - if(statistics=='quantile'){ - # fix repeated probs - testprobs=sort(unique(testprobs)) - }else{ - testprobs=0.5 - } - regionsFeatures_test=IWTomicsTest(regionsFeatures,id_region1,id_region2,id_features_subset, - statistics=statistics,probs=testprobs,B=B) - # create adjustedvaluematrix output - for(test in seq_along(id_region1)){ - for(id_feature in id_features_subset){ - write(paste0('Test: ',id_region1[test],' vs ',id_region2[test],', on feature ',id_feature), - file=adjustedpvaluematrix,append=TRUE,sep='\t') - pval=regionsFeatures_test@test$result[[test]][[id_feature]]$adjusted_pval_matrix - row.names(pval)=paste('Scale',rev(seq_len(nrow(pval)))) - write.table(pval,file=adjustedpvaluematrix,append=TRUE,sep='\t',quote=FALSE,row.names=TRUE,col.names=FALSE) - write('',file=adjustedpvaluematrix,append=TRUE,sep='\t') - } - } - }, error = function(err) { - quit(save="no", status=40) #error testing - stop(err) - }) - - # plot test results - pdf(iwtomicsrespdf,width=5,height=7) - if(plottype=='boxplot'){ - # fix repeated probs - probs=sort(unique(probs)) - }else{ - probs=c(0.25,0.5,0.75) - } - plotTest(regionsFeatures_test,alpha=testalpha,type=plottype,probs=probs,average=average,size=size,ask=FALSE) - dev.off() - - # plot summary results - if(groupby!='none'){ - pdf(iwtomicssumpdf,width=15,height=10) - plotSummary(regionsFeatures_test,alpha=summaryalpha,only_significant=only_significant,groupby=groupby,ask=FALSE,append=TRUE) - dev.off() - } - - # create output - write.table(as.data.frame(t(paste(id_region1,'vs',id_region2))),file=iwtomicstests,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) - write.table(as.data.frame(t(idFeatures(regionsFeatures_test))),file=iwtomicsselectedfeatures,quote=FALSE,sep='\t',row.names=FALSE,col.names=FALSE) - save(regionsFeatures_test,file=iwtomicsrdata) -}else{ - quit(save="no", status=255) - stop("Missing IWTomics package") -} \ No newline at end of file
--- a/testandplot.xml Wed May 03 11:10:18 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,235 +0,0 @@ -<tool id="testandplot" name="IWTomics Test" version="0.1.0"> - <description>and Plot</description> - <command interpreter="Rscript"> -<![CDATA[ - testandplot.R adjustedpvaluematrix="${adjustedpvaluematrix}" iwtomicsrespdf="${iwtomicsrespdf}" iwtomicssumpdf="${iwtomicssumpdf}" iwtomicsrdata="${iwtomicsrdata}" iwtomicstests="${iwtomicstests}" iwtomicsselectedfeatures="${iwtomicsselectedfeatures}" - regionids="${regionids}" - featureids="${featureids}" - rdatafile="${rdata}" - - #set region1 = '","'.join( [ str( $r.region0 ) for $r in $regionssection.regions ] ) - #set region2 = '","'.join( [ str( $r.region1 ) for $r in $regionssection.regions ] ) - region1="c(${region1})" - region2="c(${region2})" - - features_subset="c(${featureslist})" - - statistics="'${conditionalstatistics.statistics}'" - #if $conditionalstatistics.statistics == "quantile": - #set probabilities = '","'.join( [ str( $p.qprob ) for $p in $conditionalstatistics.quantilesection.qprobabilities ] ) - testprobs="c(${probabilities})" - #end if - - B="${permutations}" - - testalpha="${plotres.alpha}" - average="${plotres.average}" - size="${plotres.size}" - plottype="'${plotres.conditionalplottype.plottype}'" - #if $plotres.conditionalplottype.plottype == 'boxplot': - #set probs = '","'.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] ) - #if $probs != "": - probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})" - #else: - probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})" - #end if - #end if - - groupby="'${plotsum.conditionalgroupby.groupby}'" - #if $plotsum.conditionalgroupby.groupby == "test": - summaryalpha="${plotsum.conditionalgroupby.testalphaplot}" - only_significant="${plotsum.conditionalgroupby.testonlysig}" - #elif $plotsum.conditionalgroupby.groupby == "feature": - summaryalpha="${plotsum.conditionalgroupby.featurealphaplot}" - only_significant="${plotsum.conditionalgroupby.featureonlysig}" - #end if - - >& /dev/null -]]> - <!-- - to print the stack add the following line at the end of the command and enable the corresponding entry in output - 2> "${stackerr}" - --> - </command> - - <inputs> - <!-- RData --> - <param format="rdata" name="rdata" type="data" label="Select IWTomicsData object" help="File created by 'IWTomics Load Smooth and Plot'." /> - <!-- region IDs --> - <param format="tabular" name="regionids" type="data" label="Select region dataset IDs" help="File created by 'IWTomics Load Smooth and Plot'." /> - <!-- feature IDs --> - <param format="tabular" name="featureids" type="data" label="Select feature IDs" help="File created by 'IWTomics Load Smooth and Plot'." /> - - <!-- repeat region ids --> - <section name="regionssection" title="Select regions for Interval-Wise Testing" expanded="True" help="IDs of the region datasets to be tested."> - <repeat name="regions" title="Two-sample test" min="1"> - <param name="region0" type="data_column" data_ref="regionids" numerical="False" label="Region 1" multiple="False" use_header_names="True" /> - <param name="region1" type="data_column" data_ref="regionids" numerical="False" label="Region 2" multiple="False" use_header_names="True" /> - </repeat> - </section> - - <!-- feature ids list --> - <param name="featureslist" type="data_column" data_ref="featureids" numerical="False" label="Select features" multiple="True" use_header_names="True" help="IDs of the features to be tested." /> - - <!-- conditional statistics --> - <conditional name="conditionalstatistics"> - <!-- statistics --> - <param name="statistics" type="select" label="Test statistics"> - <option value="mean">Mean difference</option> - <option value="median">Median difference</option> - <option value="variance">Variance ratio</option> - <option value="quantile">Quantile difference(s)</option> - </param> - <!-- conditional choice: statistics=quantile --> - <when value="quantile"> - <section name="quantilesection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantiles in test statistics."> - <repeat name="qprobabilities" title="Probabilities" min="1"> - <param name="qprob" size="4" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> - </repeat> - </section> - </when> - </conditional> - - <!-- permutations --> - <param name="permutations" type="integer" value="1000" min="1" label="Number of permutations" /> - - <!-- plot IWTomics results --> - <section name="plotres" title="Plot IWTomics test results" expanded="True"> - <!-- alpha --> - <param name="alpha" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> - <!-- average --> - <param name="average" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot the mean curves" /> - <!-- average --> - <param name="size" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot sample size in each position" /> - <!-- conditional plot type --> - <conditional name="conditionalplottype"> - <!-- plot type --> - <param name="plottype" type="select" label="Plot type"> - <option value="boxplot">Pointwise quantile curves (boxplot)</option> - <option value="curves">Curves (aligned)</option> - </param> - <!-- conditional choice: plottype=boxplot --> - <when value="boxplot"> - <section name="probabilitiessection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantile curves to be drawn."> - <param name="prob0" size="3" type="float" value="0.25" min="0.0" max="1.0" label="Probability" /> - <param name="prob1" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> - <param name="prob2" size="3" type="float" value="0.75" min="0.0" max="1.0" label="Probability" /> - <repeat name="probabilities" title="Probabilities"> - <param name="prob" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> - </repeat> - </section> - </when> - </conditional> - </section> - - <!-- summary plot --> - <section name="plotsum" title="Summary plot" expanded="True"> - <!-- conditional group by --> - <conditional name="conditionalgroupby"> - <!-- group by --> - <param name="groupby" type="select" label="Group by" help="How tests should be grouped."> - <option value="none">No plot</option> - <option value="test">Group by test</option> - <option value="feature">Group by feature</option> - </param> - <when value="test"> - <param name="testalphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> - <param name="testonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> - </when> - <when value="feature"> - <param name="featurealphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> - <param name="featureonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> - </when> - </conditional> - </section> - </inputs> - - <outputs> - <!--<data format="txt" name="stackerr" label="iwtomics.testandplot.stackerr.txt" from_work_dir="iwtomics.testandplot.stackerr.txt" />--> - <data format="txt" name="adjustedpvaluematrix" label="${tool.name} on ${on_string}: Adjusted p-value Matrix" from_work_dir="iwtomics.testandplot.adjustedpvalue.matrix.txt" /> - <data format="pdf" name="iwtomicsrespdf" label="${tool.name} on ${on_string}: Plotted Test Results" from_work_dir="iwtomics.testandplot.iwtomicstestresults.pdf" /> - <data format="pdf" name="iwtomicssumpdf" label="${tool.name} on ${on_string}: Summary Plot" from_work_dir="iwtomics.testandplot.summaryplot.pdf" /> - <data format="rdata" name="iwtomicsrdata" label="${tool.name} on ${on_string}: IWTomicsData Object with Test Results" from_work_dir="iwtomics.testandplot.RData" /> - <data format="tabular" name="iwtomicstests" label="${tool.name} on ${on_string}: Test IDs" from_work_dir="iwtomics.testandplot.tests.txt" /> - <data format="tabular" name="iwtomicsselectedfeatures" label="${tool.name} on ${on_string}: Feature IDs" from_work_dir="iwtomics.testandplot.selectedfeatures.txt" /> - </outputs> - - <help> -This tool statistically evaluates differences in genomic features between groups of regions along the genome. -In particular, it implements the Interval-Wise Testing for omics data, an extended version of the Interval-Wise -Testing for functional data presented in Pini and Vantini (2017). It allows to perform multiple two sample permutation -tests between pairs of region datasets, on several features. It returns the adjusted p-value curves for every test -and all possible scales. Moreover, it creates a graphical representation of the Interval-Wise Testing results -and a summary plot (optional) with p-values at the maximum scale. The tool *IWTomics Plot with Threshold on Test Scale* -permits to select the scale to be used in the plots. - ------ - -**Input files** - -RData file with the IWTomicsData object, tabular files with region dataset IDs and feature IDs. -These files are created by the tool *IWTomics Load Smooth and Plot*. - ------ - -**Output** - -The tool returns: - -1. TXT file with an adjusted p-value matrix for every test performed. Each matrix contains a p-value curve (row) for every scale considered in the test; -2. PDF file with the plotted test results; -3. PDF file with the summary plot; -4. RData with the IWTomicsData object with the test results; -5. Test identifiers; -6. Feature identifiers. - -4-6 can be used as input of the tool *IWTomics Plot with Threshold on Test Scale* - ------ - -.. class:: infomark - -**Notes** - -This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). - -It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects. -The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). - -.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf - </help> - - <citations> - <citation type="bibtex"> - @ARTICLE{ - iwt-functional-data, - author = {A Pini and S. Vantini}, - title = {Interval-Wise Testing for functional data}, - journal = {Journal of Nonparametric Statistics}, - year = {2017}, - volume = {29}, - number = {2}, - pages = {407-424} - } - </citation> - <citation type="bibtex"> - @MANUAL{ - iwtomics, - author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, - title = {IWTomics: Interval-Wise Testing for Omics Data}, - note = {R package version 0.99.12}, - year = {2017} - } - </citation> - </citations> - - <stdio> - <exit_code range="-1" source="both" level="fatal" description="Missing IWTomics package. Please be sure to have it installed before using this tool." /> - <exit_code range="10" source="both" level="fatal" description="Wrong region ids." /> - <exit_code range="20" source="both" level="fatal" description="Wrong feature ids." /> - <exit_code range="30" source="both" level="fatal" description="Same test repeated multiple times." /> - <exit_code range="40" source="both" level="fatal" description="Testing error." /> - <regex match="Error" source="both" level="fatal" description="An error has occurred. Please try again." /> - </stdio> - -</tool>