# HG changeset patch # User fcaramia # Date 1347511873 14400 # Node ID 734516a21b5220c5291fe11478a55038a71ff358 # Parent 3aadcea3347bb745c8705748425683edfea971d2 Uploaded diff -r 3aadcea3347b -r 734516a21b52 htseq.xml --- a/htseq.xml Thu Sep 13 00:50:59 2012 -0400 +++ b/htseq.xml Thu Sep 13 00:51:13 2012 -0400 @@ -1,5 +1,5 @@ - + - Create a digital expression matrix by counting reads in features with htseq-count htseq.pl -m $MODE -s $STRANDED -a $MINAQUAL -t $FEATURETYPE -i $IDATTR -g $gff_file -o $output -r $report $REDUCE ## Inputs. @@ -70,7 +70,7 @@ The following figure illustrates the effect of these three modes: -.. image:: ./static/operation_icons/count_modes.png +.. image:: http://www-huber.embl.de/users/anders/HTSeq/doc/_images/count_modes.png The strandedness of the assay may also be set. For stranded=no, a read is considered overlapping with a feature regardless of whether it is mapped to the same or the opposite strand as the feature. For stranded=yes and single-end reads, the read has to be mapped to the same strand as the feature. For paired-end reads, the first read has to be on the same strand and the second read on the opposite strand. For stranded=reverse, these rules are reversed. @@ -90,6 +90,12 @@ * not_aligned: reads in the Sam/Bam file without alignment * alignment_not_unique: reads with more than one reported alignment. These reads are recognized from the NH optional SAM field tag. (If the aligner does not set this field, multiply aligned reads will be counted multiple times.) +**Reference** + +http://www-huber.embl.de/users/anders/HTSeq/doc/overview.html + + +