Mercurial > repos > florianbegusch > qiime2_all
diff qiime_deblur_denoise-other.xml @ 0:09b7bcb72fa7 draft
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author | florianbegusch |
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date | Thu, 24 May 2018 02:11:44 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime_deblur_denoise-other.xml Thu May 24 02:11:44 2018 -0400 @@ -0,0 +1,122 @@ +<?xml version="1.0" ?> +<tool id="qiime_deblur_denoise-other" name="qiime deblur denoise-other" version="2018.4"> + <description> - Deblur sequences using a user-specified positive filter.</description> + <requirements> + <requirement type="package" version="2018.4">qiime2</requirement> + </requirements> + <command><![CDATA[ + qiime deblur denoise-other --i-demultiplexed-seqs=$idemultiplexedseqs --p-trim-length="$ptrimlength" --i-reference-seqs=$ireferenceseqs + + #if str($cmdconfig) != 'None': + --cmd-config=$cmdconfig + #end if + + #if $phashedfeatureids: + --p-hashed-feature-ids + #else + --p-no-hashed-feature-ids + #end if + + #if $pindelprob: + --p-indel-prob=$pindelprob + #end if + + #if $pindelmax: + --p-indel-max=$pindelmax + #end if + + #if $pmeanerror: + --p-mean-error=$pmeanerror + #end if + + --o-representative-sequences=orepresentativesequences --o-table=otable --o-stats=ostats + + #set $pjobstostart = '${GALAXY_SLOTS:-4}' + + #if str($pjobstostart): + --p-jobs-to-start="$pjobstostart" + #end if + + #if $pminreads: + --p-min-reads=$pminreads + #end if + + #if $pminsize: + --p-min-size=$pminsize + #end if + + --p-sample-stats + ; + cp orepresentativesequences.qza $orepresentativesequences; + cp otable.qza $otable; + cp ostats.qza $ostats; + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-demultiplexed-seqs: SampleData[JoinedSequencesWithQuality | PairedEndSequencesWithQuality | SequencesWithQuality] The demultiplexed sequences to be denoised. [required]" name="idemultiplexedseqs" optional="False" type="data"/> + <param format="qza,no_unzip.zip" label="--i-reference-seqs: FeatureData[Sequence] Positive filtering database. Keep all sequences aligning to these sequences. [required]" name="ireferenceseqs" optional="False" type="data"/> + <param label="--p-trim-length: Sequence trim length, specify -1 to disable trimming. [required]" name="ptrimlength" optional="False" type="text"/> + <param label="--p-mean-error: The mean per nucleotide error, used for original sequence estimate. [default: 0.005]" name="pmeanerror" optional="True" type="float" value="0.005"/> + <param label="--p-indel-prob: Insertion/deletion (indel) probability (same for N indels). [default: 0.01]" name="pindelprob" optional="True" type="float" value="0.01"/> + <param label="--p-indel-max: Maximum number of insertion/deletions. [default: 3]" name="pindelmax" optional="True" type="integer" value="3"/> + <param label="--p-min-reads: Retain only features appearing at least min_reads times across all samples in the resulting feature table. [default: 10]" name="pminreads" optional="True" type="integer" value="10"/> + <param label="--p-min-size: In each sample, discard all features with an abundance less than min_size. [default: 2]" name="pminsize" optional="True" type="integer" value="2"/> + <param label="--p-hashed-feature-ids: --p-no-hashed-feature-ids If true, hash the feature IDs. [default: True]" name="phashedfeatureids" checked="True" type="boolean"/> + <param label="--cmd-config: Use config file for command options" name="cmdconfig" optional="True" type="data"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: representative-sequences.qza" name="orepresentativesequences"/> + <data format="qza" label="${tool.name} on ${on_string}: table.qza" name="otable"/> + <data format="qza" label="${tool.name} on ${on_string}: stats.qza" name="ostats"/> + </outputs> + <help><![CDATA[ +Deblur sequences using a user-specified positive filter. +--------------------------------------------------------- + +Perform sequence quality control for Illumina data using the Deblur +workflow, including positive alignment-based filtering. Only forward reads +are supported at this time. This mode of execution is particularly useful +when operating on non-16S data. For example, to apply Deblur to 18S data, +you would want to specify a reference composed of 18S sequences in order to +filter out sequences which do not appear to be 18S. The assessment is +performed by local alignment using SortMeRNA with a permissive e-value +threshold. + +Parameters +---------- +demultiplexed_seqs : SampleData[JoinedSequencesWithQuality | PairedEndSequencesWithQuality | SequencesWithQuality] + The demultiplexed sequences to be denoised. +reference_seqs : FeatureData[Sequence] + Positive filtering database. Keep all sequences aligning to these + sequences. +trim_length : Int + Sequence trim length, specify -1 to disable trimming. +sample_stats : Bool, optional + If true, gather stats per sample. +mean_error : Float, optional + The mean per nucleotide error, used for original sequence estimate. +indel_prob : Float, optional + Insertion/deletion (indel) probability (same for N indels). +indel_max : Int, optional + Maximum number of insertion/deletions. +min_reads : Int, optional + Retain only features appearing at least min_reads times across all + samples in the resulting feature table. +min_size : Int, optional + In each sample, discard all features with an abundance less than + min_size. +jobs_to_start : Int, optional + Number of jobs to start (if to run in parallel). +hashed_feature_ids : Bool, optional + If true, hash the feature IDs. + +Returns +------- +table : FeatureTable[Frequency] + The resulting denoised feature table. +representative_sequences : FeatureData[Sequence] + The resulting feature sequences. +stats : DeblurStats + Per-sample stats if requested. + ]]> + </help> +</tool>