Mercurial > repos > florianbegusch > qiime2_all
diff qiime_vsearch_join-pairs.xml @ 0:09b7bcb72fa7 draft
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author | florianbegusch |
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date | Thu, 24 May 2018 02:11:44 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime_vsearch_join-pairs.xml Thu May 24 02:11:44 2018 -0400 @@ -0,0 +1,152 @@ +<?xml version="1.0" ?> +<tool id="qiime_vsearch_join-pairs" name="qiime vsearch join-pairs" version="2018.4"> + <description> - Join paired-end reads.</description> + <requirements> + <requirement type="package" version="2018.4">qiime2</requirement> + </requirements> + <command> + <![CDATA[ + qiime vsearch join-pairs --i-demultiplexed-seqs=$idemultiplexedseqs + + #if $pminovlen: + --p-minovlen=$pminovlen + #end if + + #if str($ptruncqual): + --p-truncqual="$ptruncqual" + #end if + + #if str($cmdconfig) != 'None': + --cmd-config=$cmdconfig + #end if + + #if str($pminmergelen): + --p-minmergelen="$pminmergelen" + #end if + + #if $pmaxdiffs: + --p-maxdiffs=$pmaxdiffs + #end if + + #if str($pqminout): + --p-qminout="$pqminout" + #end if + + #if str($pmaxmergelen): + --p-maxmergelen="$pmaxmergelen" + #end if + + #if str($pmaxns): + --p-maxns="$pmaxns" + #end if + + #if $pallowmergestagger: + --p-allowmergestagger + #else + --p-no-allowmergestagger + #end if + + #if $pminlen: + --p-minlen=$pminlen + #end if + + #if $pqmax: + --p-qmax=$pqmax + #end if + + #if str($pmaxee): + --p-maxee="$pmaxee" + #end if + + #if str($pqmin): + --p-qmin="$pqmin" + #end if + + #if $pqmaxout: + --p-qmaxout=$pqmaxout + #end if + --o-joined-sequences=ojoinedsequences; + + cp ojoinedsequences.qza $ojoinedsequences; + ]]> + </command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-demultiplexed-seqs: SampleData[PairedEndSequencesWithQuality] The demultiplexed paired-end sequences to be joined. [required]" name="idemultiplexedseqs" optional="False" type="data"/> + + <param label="--p-truncqual: Truncate sequences at the first base with the specified quality score value or lower. [optional]" name="ptruncqual" optional="True" type="text"/> + + <param label="--p-minlen: Sequences shorter than minlen after truncation are discarded. [default: 1]" name="pminlen" optional="True" type="integer" value="1"/> + <param label="--p-maxns: Sequences with more than maxns N characters are discarded. [optional]" name="pmaxns" optional="True" type="text"/> + + <param label="--p-allowmergestagger: --p-no-allowmergestagger Allow joining of staggered read pairs. [default: False]" name="pallowmergestagger" checked="False" type="boolean"/> + + <param label="--p-minovlen: Minimum overlap length of forward and reverse reads for joining. [default: 10]" name="pminovlen" optional="True" type="integer" value="10"/> + <param label="--p-maxdiffs: Maximum number of mismatches in the forward/reverse read overlap for joining. [default: 10]" name="pmaxdiffs" optional="True" type="integer" value="10"/> + + <param label="--p-minmergelen: Minimum length of the joined read to be retained. [optional]" name="pminmergelen" optional="True" type="text"/> + <param label="--p-maxmergelen: Maximum length of the joined read to be retained. [optional]" name="pmaxmergelen" optional="True" type="text"/> + + <param label="--p-maxee: Maximum number of expected errors in the joined read to be retained. [optional]" name="pmaxee" optional="True" type="text"/> + + <param label="--p-qmin: The minimum allowed quality score in the input. [default: 0]" name="pqmin" optional="True" type="text" value="0"/> + <param label="--p-qminout: The minimum allowed quality score to use in output. [default: 0]" name="pqminout" optional="True" type="text" value="0"/> + + <param label="--p-qmax: The maximum allowed quality score in the input. [default: 41]" name="pqmax" optional="True" type="integer" value="41"/> + <param label="--p-qmaxout: The maximum allowed quality score to use in output. [default: 41]" name="pqmaxout" optional="True" type="integer" value="41"/> + + <param label="--cmd-config: Use config file for command options" name="cmdconfig" optional="True" type="data"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: joined-sequences.qza" name="ojoinedsequences"/> + </outputs> + <help> + <![CDATA[ +Join paired-end reads. +----------------------- + +Join paired-end sequence reads using vsearch's merge_pairs function. The +qmin, qminout, qmax, and qmaxout parameters should only need to be modified +when working with older fastq sequence data. See the vsearch documentation +for details on how paired-end joining is performed, and for more +information on the parameters to this method. + +Parameters +---------- +demultiplexed_seqs : SampleData[PairedEndSequencesWithQuality] + The demultiplexed paired-end sequences to be joined. +truncqual : Int % Range(0, None), optional + Truncate sequences at the first base with the specified quality score + value or lower. +minlen : Int % Range(0, None), optional + Sequences shorter than minlen after truncation are discarded. +maxns : Int % Range(0, None), optional + Sequences with more than maxns N characters are discarded. +allowmergestagger : Bool, optional + Allow joining of staggered read pairs. +minovlen : Int % Range(0, None), optional + Minimum overlap length of forward and reverse reads for joining. +maxdiffs : Int % Range(0, None), optional + Maximum number of mismatches in the forward/reverse read overlap for + joining. +minmergelen : Int % Range(0, None), optional + Minimum length of the joined read to be retained. +maxmergelen : Int % Range(0, None), optional + Maximum length of the joined read to be retained. +maxee : Float % Range(0.0, None), optional + Maximum number of expected errors in the joined read to be retained. +qmin : Int % Range(-5, 2, inclusive_end=True), optional + The minimum allowed quality score in the input. +qminout : Int % Range(-5, 2, inclusive_end=True), optional + The minimum allowed quality score to use in output. +qmax : Int % Range(40, 41, inclusive_end=True), optional + The maximum allowed quality score in the input. +qmaxout : Int % Range(40, 41, inclusive_end=True), optional + The maximum allowed quality score to use in output. + +Returns +------- +joined_sequences : SampleData[JoinedSequencesWithQuality] + The joined sequences. + ]]> + </help> +</tool>