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view qiime_feature-classifier_classify-consensus-vsearch.xml @ 0:09b7bcb72fa7 draft
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author | florianbegusch |
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date | Thu, 24 May 2018 02:11:44 -0400 |
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<?xml version="1.0" ?> <tool id="qiime_feature-classifier_classify-consensus-vsearch" name="qiime feature-classifier classify-consensus-vsearch" version="2018.4"> <description> - VSEARCH consensus taxonomy classifier</description> <requirements> <requirement type="package" version="2018.4">qiime2</requirement> </requirements> <command><![CDATA[ qiime feature-classifier classify-consensus-vsearch --i-query=$iquery #if str( $id_to_taxonomy_fp.selector ) == 'history' #set $tax = $id_to_taxonomy_fp.taxonomy_fp --i-reference-taxonomy '$tax' #else: #set $tax = $id_to_taxonomy_fp.taxonomy_fp.fields.path --i-reference-taxonomy '$tax' #end if --i-reference-reads=$ireferencereads #if $ppercidentity: --p-perc-identity=$ppercidentity #end if #if str($cmdconfig) != 'None': --cmd-config=$cmdconfig #end if #if $pminconsensus: --p-min-consensus=$pminconsensus #end if #if str($pstrand) != 'None': --p-strand=$pstrand #end if --o-classification=oclassification #if str($punassignablelabel): --p-unassignable-label="$punassignablelabel" #end if #set $pthreads = '${GALAXY_SLOTS:-4}' --p-threads="$pthreads" #if $pmaxaccepts: --p-maxaccepts=$pmaxaccepts #end if ; cp oclassification.qza $oclassification; ]]></command> <inputs> <param format="qza,no_unzip.zip" label="--i-query: FeatureData[Sequence] Sequences to classify taxonomically. [required]" name="iquery" optional="False" type="data"/> <param format="qza,no_unzip.zip" label="--i-reference-reads: FeatureData[Sequence] reference sequences. [required]" name="ireferencereads" optional="False" type="data"/> <conditional name="id_to_taxonomy_fp" optional="True"> <param name="selector" type="select" label="Reference taxonomy to query"> <option value="cached">Public databases</option> <option value="history">Databases from your history</option> </param> <when value="cached"> <param argument="--taxonomy_fp" label="Reference taxonomy" type="select" optional="True"> <options from_data_table="qiime_taxonomy" /> </param> </when> <when value="history"> <param argument="--taxonomy_fp" type="data" format="qza,no_unzip.zip" label="Reference databases" optional="True" /> </when> </conditional> <param label="--p-maxaccepts: Maximum number of hits to keep for each query. Must be in range [0, infinity]. [default: 10]" name="pmaxaccepts" optional="True" type="integer" value="10"/> <param label="--p-perc-identity: Reject match if percent identity to query is lower. Must be in range [0.0, 1.0]. [default: 0.8]" name="ppercidentity" optional="True" type="float" value="0.8"/> <param label="--p-strand: [both|plus] Align against reference sequences in forward ('plus') or both directions ('both'). [default: both]" name="pstrand" optional="True" type="select"> <option selected="True" value="both">both</option> <option value="plus">plus</option> </param> <param label="--p-min-consensus: Minimum fraction of assignments must match top hit to be accepted as consensus assignment. Must be in range (0.5, 1.0]. [default: 0.51]" name="pminconsensus" optional="True" type="float" value="0.51"/> <param label="--p-unassignable-label: [default: Unassigned]" name="punassignablelabel" optional="True" type="text" value="Unassigned"/> <param label="--cmd-config: Use config file for command options" name="cmdconfig" optional="True" type="data"/> </inputs> <outputs> <data format="qza" label="${tool.name} on ${on_string}: classification.qza" name="oclassification"/> </outputs> <help><![CDATA[ VSEARCH consensus taxonomy classifier ------------------------------------- Assign taxonomy to query sequences using VSEARCH. Performs VSEARCH global alignment between query and reference_reads, then assigns consensus taxonomy to each query sequence from among maxaccepts top hits, min_consensus of which share that taxonomic assignment. Parameters ---------- query : FeatureData[Sequence] Sequences to classify taxonomically. reference_reads : FeatureData[Sequence] reference sequences. reference_taxonomy : FeatureData[Taxonomy] reference taxonomy labels. maxaccepts : Int % Range(1, None), optional Maximum number of hits to keep for each query. Must be in range [0, infinity]. perc_identity : Float % Range(0.0, 1.0, inclusive_end=True), optional Reject match if percent identity to query is lower. Must be in range [0.0, 1.0]. strand : Str % Choices({'both', 'plus'}), optional Align against reference sequences in forward ("plus") or both directions ("both"). min_consensus : Float % Range(0.5, 1.0, inclusive_start=False, inclusive_end=True), optional Minimum fraction of assignments must match top hit to be accepted as consensus assignment. Must be in range (0.5, 1.0]. unassignable_label : Str, optional \ Returns ------- classification : FeatureData[Taxonomy] The resulting taxonomy classifications. ]]> </help> </tool>