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1 <?xml version="1.0" ?>
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2 <tool id="qiime_demux_subsample-single" name="qiime demux subsample-single" version="2019.4">
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3 <description> - Subsample single-end sequences without replacement.</description>
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4 <requirements>
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5 <requirement type="package" version="2019.4">qiime2</requirement>
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6 </requirements>
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7 <command><![CDATA[
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8 qiime demux subsample-single
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9
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10 --i-sequences=$isequences
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11 --p-fraction="$pfraction"
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12
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13 --o-subsampled-sequences=osubsampledsequences
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14 ;
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15 cp osubsampledsequences.qza $osubsampledsequences
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16 ]]></command>
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17 <inputs>
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18 <param format="qza,no_unzip.zip" label="--i-sequences: ARTIFACT SampleData[SequencesWithQuality | PairedEndSequencesWithQuality] The demultiplexed sequences to be subsampled. [required]" name="isequences" optional="False" type="data"/>
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19 <param label="--p-fraction: PROPORTION Range(0, 1, inclusive_start=False) The fraction of sequences to retain in subsample. [required]" name="pfraction" optional="False" exclude_min="True" min="0" max="1" value="" type="float"/>
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20 </inputs>
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21 <outputs>
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22 <data format="qza" label="${tool.name} on ${on_string}: subsampledsequences.qza" name="osubsampledsequences"/>
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23 </outputs>
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24 <help><![CDATA[
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25 Subsample single-end sequences without replacement.
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26 ###################################################
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27
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28 Generate a random subsample of single-end sequences containing
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29 approximately the fraction of input sequences specified by the fraction
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30 parameter. The number of output samples will always be equal to the number
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31 of input samples, even if some of those samples contain no sequences after
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32 subsampling.
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33
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34 Parameters
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35 ----------
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36 sequences : SampleData[SequencesWithQuality | PairedEndSequencesWithQuality]
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37 The demultiplexed sequences to be subsampled.
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38 fraction : Float % Range(0, 1, inclusive_start=False)
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39 The fraction of sequences to retain in subsample.
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40
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41 Returns
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42 -------
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43 subsampled_sequences : SampleData[SequencesWithQuality]
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44 The subsampled sequences.
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45 ]]></help>
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46 <macros>
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47 <import>qiime_citation.xml</import>
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48 </macros>
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49 <expand macro="qiime_citation"/>
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50 </tool>
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