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1 <?xml version="1.0" ?>
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2 <tool id="qiime_cutadapt_demux-single" name="qiime cutadapt demux-single" version="2019.4">
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3 <description> - Demultiplex single-end sequence data with barcodes in- sequence.</description>
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4 <requirements>
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5 <requirement type="package" version="2019.4">qiime2</requirement>
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6 </requirements>
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7 <command><![CDATA[
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8 qiime cutadapt demux-single
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9
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10 --i-seqs=$iseqs
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4
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11
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12
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13
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14 #if '__sq__' in str($mbarcodescolumn):
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15 #set $mbarcodescolumn_temp = $mbarcodescolumn.replace('__sq__', "'")
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16 #set $mbarcodescolumn = $mbarcodescolumn_temp
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17 #end if
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18
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19 --m-barcodes-column="$mbarcodescolumn"
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20
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21
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22 --o-per-sample-sequences=opersamplesequences
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23 --o-untrimmed-sequences=ountrimmedsequences
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24
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2
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25
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26
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27
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28
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29
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30 #if $input_files_mbarcodesfile:
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31 #def list_dict_to_string(list_dict):
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32 #set $file_list = list_dict[0]['additional_input'].__getattr__('file_name')
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33 #for d in list_dict[1:]:
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34 #set $file_list = $file_list + ',' + d['additional_input'].__getattr__('file_name')
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35 #end for
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36 #return $file_list
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37 #end def
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38 --m-barcodes-file=$list_dict_to_string($input_files_mbarcodesfile)
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2
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39 #end if
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40
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41
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42 #if $perrorrate:
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43 --p-error-rate=$perrorrate
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44 #end if
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45
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46 ;
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47
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48 cp opersamplesequences.qza $opersamplesequences;
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49 cp ountrimmedsequences.qza $ountrimmedsequences
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50 ]]></command>
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51 <inputs>
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52 <param format="qza,no_unzip.zip" label="--i-seqs: ARTIFACT MultiplexedSingleEndBarcodeInSequence The single-end sequences to be demultiplexed. [required]" name="iseqs" optional="False" type="data"/>
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53 <repeat name="input_files_mbarcodesfile" optional="False" title="--m-barcodes-file">
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54 <param label="--m-barcodes-file: Metadata file or artifact viewable as metadata. This option may be supplied multiple times to merge metadata. [required]" name="additional_input" type="data" format="tabular,qza,no_unzip.zip" />
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55 </repeat>
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56 <param label="--m-barcodes-column: COLUMN MetadataColumn[Categorical] The sample metadata column listing the per-sample barcodes. [required]" name="mbarcodescolumn" optional="False" type="text"/>
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57 <param label="--p-error-rate: PROPORTION Range(0, 1, inclusive_end=True) The level of error tolerance, specified as the maximum allowable error rate. The default value specified by cutadapt is 0.1 (=10%), which is greater than `demux emp-*`, which is 0.0 (=0%). [default: 0.1]" name="perrorrate" optional="True" type="float" min="0" max="1" exclude_max="False" value="0.1"/>
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58 </inputs>
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59 <outputs>
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60 <data format="qza" label="${tool.name} on ${on_string}: persamplesequences.qza" name="opersamplesequences"/>
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61 <data format="qza" label="${tool.name} on ${on_string}: untrimmedsequences.qza" name="ountrimmedsequences"/>
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62 </outputs>
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63 <help><![CDATA[
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64 Demultiplex single-end sequence data with barcodes in-sequence.
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65 ###############################################################
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66
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67 Demultiplex sequence data (i.e., map barcode reads to sample ids). Barcodes
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68 are expected to be located within the sequence data (versus the header, or
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69 a separate barcode file).
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70
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71 Parameters
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72 ----------
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73 seqs : MultiplexedSingleEndBarcodeInSequence
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74 The single-end sequences to be demultiplexed.
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75 barcodes : MetadataColumn[Categorical]
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76 The sample metadata column listing the per-sample barcodes.
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77 error_rate : Float % Range(0, 1, inclusive_end=True), optional
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78 The level of error tolerance, specified as the maximum allowable error
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79 rate. The default value specified by cutadapt is 0.1 (=10%), which is
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80 greater than `demux emp-*`, which is 0.0 (=0%).
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81
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82 Returns
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83 -------
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84 per_sample_sequences : SampleData[SequencesWithQuality]
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85 The resulting demultiplexed sequences.
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86 untrimmed_sequences : MultiplexedSingleEndBarcodeInSequence
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87 The sequences that were unmatched to barcodes.
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88 ]]></help>
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89 <macros>
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90 <import>qiime_citation.xml</import>
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91 </macros>
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92 <expand macro="qiime_citation"/>
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93 </tool>
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