|
20
|
1 <?xml version="1.0" ?>
|
|
|
2 <tool id="qiime_phylogeny_align-to-tree-mafft-fasttree" name="qiime phylogeny align-to-tree-mafft-fasttree"
|
|
|
3 version="2020.8">
|
|
|
4 <description>Build a phylogenetic tree using fasttree and mafft alignment</description>
|
|
|
5 <requirements>
|
|
|
6 <requirement type="package" version="2020.8">qiime2</requirement>
|
|
|
7 </requirements>
|
|
|
8 <command><![CDATA[
|
|
|
9 qiime phylogeny align-to-tree-mafft-fasttree
|
|
|
10
|
|
|
11 --i-sequences=$isequences
|
|
|
12
|
|
|
13 #if str($pnthreads) != 'None':
|
|
|
14 --p-n-threads=$pnthreads
|
|
|
15 #end if
|
|
|
16
|
|
|
17 --p-mask-max-gap-frequency=$pmaskmaxgapfrequency
|
|
|
18
|
|
|
19 --p-mask-min-conservation=$pmaskminconservation
|
|
|
20
|
|
|
21 #if $pparttree:
|
|
|
22 --p-parttree
|
|
|
23 #end if
|
|
|
24
|
|
|
25 --o-alignment=oalignment
|
|
|
26
|
|
|
27 --o-masked-alignment=omaskedalignment
|
|
|
28
|
|
|
29 --o-tree=otree
|
|
|
30
|
|
|
31 --o-rooted-tree=orootedtree
|
|
|
32
|
|
|
33 #if str($examples) != 'None':
|
|
|
34 --examples=$examples
|
|
|
35 #end if
|
|
|
36
|
|
|
37 ;
|
|
|
38 cp orootedtree.qza $orootedtree
|
|
|
39
|
|
|
40 ]]></command>
|
|
|
41 <inputs>
|
|
|
42 <param format="qza,no_unzip.zip" label="--i-sequences: ARTIFACT FeatureData[Sequence] The sequences to be used for creating a fasttree based rooted phylogenetic tree. [required]" name="isequences" optional="False" type="data" />
|
|
|
43 <param label="--p-n-threads: " name="pnthreads" optional="True" type="select">
|
|
|
44 <option selected="True" value="None">Selection is Optional</option>
|
|
|
45 <option value="Int % Range(1">Int % Range(1</option>
|
|
|
46 <option value="None">None</option>
|
|
|
47 </param>
|
|
|
48 <param exclude_max="False" label="--p-mask-max-gap-frequency: PROPORTION Range(0, 1, inclusive_end=True) The maximum relative frequency of gap characters in a column for the column to be retained. This relative frequency must be a number between 0.0 and 1.0 (inclusive), where 0.0 retains only those columns without gap characters, and 1.0 retains all columns regardless of gap character frequency. This value is used when masking the aligned sequences. [default: 1.0]" max="1" min="0" name="pmaskmaxgapfrequency" optional="True" type="float" value="1.0" />
|
|
|
49 <param exclude_max="False" label="--p-mask-min-conservation: PROPORTION Range(0, 1, inclusive_end=True) The minimum relative frequency of at least one non-gap character in a column for that column to be retained. This relative frequency must be a number between 0.0 and 1.0 (inclusive). For example, if a value of 0.4 is provided, a column will only be retained if it contains at least one character that is present in at least 40% of the sequences. This value is used when masking the aligned sequences. [default: 0.4]" max="1" min="0" name="pmaskminconservation" optional="True" type="float" value="0.4" />
|
|
|
50 <param label="--p-parttree: --p-parttree: / --p-no-parttree This flag is required if the number of sequences being aligned are larger than 1000000. Disabled by default. [default: False]" name="pparttree" selected="False" type="boolean" />
|
|
|
51 <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" />
|
|
|
52
|
|
|
53 </inputs>
|
|
|
54
|
|
|
55 <outputs>
|
|
|
56 <data format="qza" label="${tool.name} on ${on_string}: alignment.qza" name="oalignment" />
|
|
|
57 <data format="qza" label="${tool.name} on ${on_string}: maskedalignment.qza" name="omaskedalignment" />
|
|
|
58 <data format="qza" label="${tool.name} on ${on_string}: tree.qza" name="otree" />
|
|
|
59 <data format="qza" label="${tool.name} on ${on_string}: rootedtree.qza" name="orootedtree" />
|
|
|
60
|
|
|
61 </outputs>
|
|
|
62
|
|
|
63 <help><![CDATA[
|
|
|
64 Build a phylogenetic tree using fasttree and mafft alignment
|
|
|
65 ###############################################################
|
|
|
66
|
|
|
67 This pipeline will start by creating a sequence alignment using MAFFT,
|
|
|
68 after which any alignment columns that are phylogenetically uninformative
|
|
|
69 or ambiguously aligned will be removed (masked). The resulting masked
|
|
|
70 alignment will be used to infer a phylogenetic tree and then subsequently
|
|
|
71 rooted at its midpoint. Output files from each step of the pipeline will be
|
|
|
72 saved. This includes both the unmasked and masked MAFFT alignment from
|
|
|
73 q2-alignment methods, and both the rooted and unrooted phylogenies from
|
|
|
74 q2-phylogeny methods.
|
|
|
75
|
|
|
76 Parameters
|
|
|
77 ----------
|
|
|
78 sequences : FeatureData[Sequence]
|
|
|
79 The sequences to be used for creating a fasttree based rooted
|
|
|
80 phylogenetic tree.
|
|
|
81 n_threads : Int % Range(1, None) | Str % Choices('auto'), optional
|
|
|
82 The number of threads. (Use `auto` to automatically use all available
|
|
|
83 cores) This value is used when aligning the sequences and creating the
|
|
|
84 tree with fasttree.
|
|
|
85 mask_max_gap_frequency : Float % Range(0, 1, inclusive_end=True), optional
|
|
|
86 The maximum relative frequency of gap characters in a column for the
|
|
|
87 column to be retained. This relative frequency must be a number between
|
|
|
88 0.0 and 1.0 (inclusive), where 0.0 retains only those columns without
|
|
|
89 gap characters, and 1.0 retains all columns regardless of gap
|
|
|
90 character frequency. This value is used when masking the aligned
|
|
|
91 sequences.
|
|
|
92 mask_min_conservation : Float % Range(0, 1, inclusive_end=True), optional
|
|
|
93 The minimum relative frequency of at least one non-gap character in a
|
|
|
94 column for that column to be retained. This relative frequency must be
|
|
|
95 a number between 0.0 and 1.0 (inclusive). For example, if a value of
|
|
|
96 0.4 is provided, a column will only be retained if it contains at
|
|
|
97 least one character that is present in at least 40% of the sequences.
|
|
|
98 This value is used when masking the aligned sequences.
|
|
|
99 parttree : Bool, optional
|
|
|
100 This flag is required if the number of sequences being aligned are
|
|
|
101 larger than 1000000. Disabled by default.
|
|
|
102
|
|
|
103 Returns
|
|
|
104 -------
|
|
|
105 alignment : FeatureData[AlignedSequence]
|
|
|
106 The aligned sequences.
|
|
|
107 masked_alignment : FeatureData[AlignedSequence]
|
|
|
108 The masked alignment.
|
|
|
109 tree : Phylogeny[Unrooted]
|
|
|
110 The unrooted phylogenetic tree.
|
|
|
111 rooted_tree : Phylogeny[Rooted]
|
|
|
112 The rooted phylogenetic tree.
|
|
|
113 ]]></help>
|
|
|
114 <macros>
|
|
|
115 <import>qiime_citation.xml</import>
|
|
|
116 </macros>
|
|
|
117 <expand macro="qiime_citation"/>
|
|
|
118 </tool> |
