Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2/qiime_cutadapt_demux-paired.xml @ 0:370e0b6e9826 draft
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author | florianbegusch |
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date | Wed, 17 Jul 2019 03:05:17 -0400 |
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children | 51025741f326 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_cutadapt_demux-paired.xml Wed Jul 17 03:05:17 2019 -0400 @@ -0,0 +1,92 @@ +<?xml version="1.0" ?> +<tool id="qiime_cutadapt_demux-paired" name="qiime cutadapt demux-paired" version="2019.4"> + <description> - Demultiplex paired-end sequence data with barcodes in- sequence.</description> + <requirements> + <requirement type="package" version="2019.4">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime cutadapt demux-paired +--i-seqs=$iseqs + +#def list_dict_to_string(list_dict): + #set $file_list = list_dict[0]['additional_input'].__getattr__('file_name') + #for d in list_dict[1:]: + #set $file_list = $file_list + ',' + d['additional_input'].__getattr__('file_name') + #end for + #return $file_list +#end def + +--m-forward-barcodes-file=$list_dict_to_string("$input_files_mforwardbarcodesfile") +--m-reverse-barcodes-file=$list_dict_to_string("$input_files_mreversebarcodesfile") + +--m-forward-barcodes-column="$mforwardbarcodescolumn" + +#if str($mreversebarcodescolumn): + --m-reverse-barcodes-column="$mreversebarcodescolumn" +#end if + +#if $perrorrate: + --p-error-rate=$perrorrate +#end if + + +--o-per-sample-sequences=opersamplesequences +--o-untrimmed-sequences=ountrimmedsequences +; + +cp opersamplesequences.qza $opersamplesequences; +cp ountrimmedsequences.qza $ountrimmedsequences + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-seqs: ARTIFACT MultiplexedPairedEndBarcodeInSequence The paired-end sequences to be demultiplexed. [required]" name="iseqs" optional="False" type="data"/> + <param label="--m-forward-barcodes-column: COLUMN MetadataColumn[Categorical] The sample metadata column listing the per-sample barcodes for the forward reads. [required]" name="mforwardbarcodescolumn" optional="False" type="text"/> + <param label="--m-reverse-barcodes-column: COLUMN MetadataColumn[Categorical] The sample metadata column listing the per-sample barcodes for the reverse reads. [optional]" name="mreversebarcodescolumn" optional="True" type="text"/> + + <repeat name="input_files_mforwardbarcodesfile" optional="False" title="--m-forward-barcodes-file"> + <param label="--m-forward-barcodes-file: Metadata file or artifact viewable as metadata. This option may be supplied multiple times to merge metadata. [required]" name="additional_input" type="data" format="tabular,qza,no_unzip.zip" /> + </repeat> + + <repeat name="input_files_mreversebarcodesfile" optional="False" title="--m-reverse-barcodes-file"> + <param label="--m-reverse-barcodes-file: Metadata file or artifact viewable as metadata. This option may be supplied multiple times to merge metadata. [required]" name="additional_input" type="data" format="tabular,qza,no_unzip.zip" /> + </repeat> + + <param label="--p-error-rate: PROPORTION Range(0, 1, inclusive_end=True) The level of error tolerance, specified as the maximum allowable error rate. [default: 0.1]" name="perrorrate" optional="True" type="float" min="0" max="1" exclude_max="False" value="0.1"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: persamplesequences.qza" name="opersamplesequences"/> + <data format="qza" label="${tool.name} on ${on_string}: untrimmedsequences.qza" name="ountrimmedsequences"/> + </outputs> + <help><![CDATA[ +Demultiplex paired-end sequence data with barcodes in-sequence. +############################################################### + +Demultiplex sequence data (i.e., map barcode reads to sample ids). Barcodes +are expected to be located within the sequence data (versus the header, or +a separate barcode file). + +Parameters +---------- +seqs : MultiplexedPairedEndBarcodeInSequence + The paired-end sequences to be demultiplexed. +forward_barcodes : MetadataColumn[Categorical] + The sample metadata column listing the per-sample barcodes for the + forward reads. +reverse_barcodes : MetadataColumn[Categorical], optional + The sample metadata column listing the per-sample barcodes for the + reverse reads. +error_rate : Float % Range(0, 1, inclusive_end=True), optional + The level of error tolerance, specified as the maximum allowable error + rate. + +Returns +------- +per_sample_sequences : SampleData[PairedEndSequencesWithQuality] + The resulting demultiplexed sequences. +untrimmed_sequences : MultiplexedPairedEndBarcodeInSequence + The sequences that were unmatched to barcodes. + ]]></help> +<macros> + <import>qiime_citation.xml</import> +</macros> +<expand macro="qiime_citation"/> +</tool>