Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2/qiime_cutadapt_demux-single.xml @ 0:370e0b6e9826 draft
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author | florianbegusch |
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date | Wed, 17 Jul 2019 03:05:17 -0400 |
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children | 51025741f326 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_cutadapt_demux-single.xml Wed Jul 17 03:05:17 2019 -0400 @@ -0,0 +1,76 @@ +<?xml version="1.0" ?> +<tool id="qiime_cutadapt_demux-single" name="qiime cutadapt demux-single" version="2019.4"> + <description> - Demultiplex single-end sequence data with barcodes in- sequence.</description> + <requirements> + <requirement type="package" version="2019.4">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime cutadapt demux-single + +--i-seqs=$iseqs +--m-barcodes-column="$mbarcodescolumn" +--o-per-sample-sequences=opersamplesequences +--o-untrimmed-sequences=ountrimmedsequences + +#def list_dict_to_string(list_dict): + #set $file_list = list_dict[0]['additional_input'].__getattr__('file_name') + #for d in list_dict[1:]: + #set $file_list = $file_list + ',' + d['additional_input'].__getattr__('file_name') + #end for + #return $file_list +#end def + +--m-barcodes-file=$list_dict_to_string("$input_files_mbarcodesfile") + +#if $perrorrate: + --p-error-rate=$perrorrate +#end if + +; + +cp opersamplesequences.qza $opersamplesequences; +cp ountrimmedsequences.qza $ountrimmedsequences + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-seqs: ARTIFACT MultiplexedSingleEndBarcodeInSequence The single-end sequences to be demultiplexed. [required]" name="iseqs" optional="False" type="data"/> + <repeat name="$input_files_mbarcodesfile" optional="False" title="--m-barcodes-file"> + <param label="--m-barcodes-file: Metadata file or artifact viewable as metadata. This option may be supplied multiple times to merge metadata. [required]" name="additional_input" type="data" format="tabular,qza,no_unzip.zip" /> + </repeat> + <param label="--m-barcodes-column: COLUMN MetadataColumn[Categorical] The sample metadata column listing the per-sample barcodes. [required]" name="mbarcodescolumn" optional="False" type="text"/> + <param label="--p-error-rate: PROPORTION Range(0, 1, inclusive_end=True) The level of error tolerance, specified as the maximum allowable error rate. The default value specified by cutadapt is 0.1 (=10%), which is greater than `demux emp-*`, which is 0.0 (=0%). [default: 0.1]" name="perrorrate" optional="True" type="float" min="0" max="1" exclude_max="False" value="0.1"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: persamplesequences.qza" name="opersamplesequences"/> + <data format="qza" label="${tool.name} on ${on_string}: untrimmedsequences.qza" name="ountrimmedsequences"/> + </outputs> + <help><![CDATA[ +Demultiplex single-end sequence data with barcodes in-sequence. +############################################################### + +Demultiplex sequence data (i.e., map barcode reads to sample ids). Barcodes +are expected to be located within the sequence data (versus the header, or +a separate barcode file). + +Parameters +---------- +seqs : MultiplexedSingleEndBarcodeInSequence + The single-end sequences to be demultiplexed. +barcodes : MetadataColumn[Categorical] + The sample metadata column listing the per-sample barcodes. +error_rate : Float % Range(0, 1, inclusive_end=True), optional + The level of error tolerance, specified as the maximum allowable error + rate. The default value specified by cutadapt is 0.1 (=10%), which is + greater than `demux emp-*`, which is 0.0 (=0%). + +Returns +------- +per_sample_sequences : SampleData[SequencesWithQuality] + The resulting demultiplexed sequences. +untrimmed_sequences : MultiplexedSingleEndBarcodeInSequence + The sequences that were unmatched to barcodes. + ]]></help> +<macros> + <import>qiime_citation.xml</import> +</macros> +<expand macro="qiime_citation"/> +</tool>