Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2/qiime_cutadapt_trim-single.xml @ 0:370e0b6e9826 draft
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author | florianbegusch |
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date | Wed, 17 Jul 2019 03:05:17 -0400 |
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children | 914fa4daf16a |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_cutadapt_trim-single.xml Wed Jul 17 03:05:17 2019 -0400 @@ -0,0 +1,151 @@ +<?xml version="1.0" ?> +<tool id="qiime_cutadapt_trim-single" name="qiime cutadapt trim-single" version="2019.4"> + <description> - Find and remove adapters in demultiplexed single-end sequences.</description> + <requirements> + <requirement type="package" version="2019.4">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime cutadapt trim-single +--i-demultiplexed-sequences=$idemultiplexedsequences + +#set $pcores = '${GALAXY_SLOTS:-4}' +#if str($pcores): +--p-cores=$pcores +#end if + +#if str($padapter): + --p-adapter="$padapter" +#end if + +#if str($pfront): + --p-front="$pfront" +#end if + +#if '__sq__' in str($panywhere): + #set $panywhere_temp = $panywhere.replace('__sq__', "'") + #set $panywhere = $panywhere_temp +#end if + +#if str($panywhere): + --p-anywhere="$panywhere" +#end if + + +#if $perrorrate: + --p-error-rate=$perrorrate +#end if + +#if $pnoindels: + --p-no-indels +#end if + +#if $ptimes: + --p-times=$ptimes +#end if + +#if $poverlap: + --p-overlap=$poverlap +#end if + +#if $pmatchreadwildcards: + --p-match-read-wildcards +#end if + +#if $pnomatchadapterwildcards: + --p-no-match-adapter-wildcards +#end if + +#if $pminimumlength: + --p-minimum-length=$pminimumlength +#end if + +#if $pdiscarduntrimmed: + --p-discard-untrimmed +#end if + +--o-trimmed-sequences=otrimmedsequences + +; +cp otrimmedsequences.qza $otrimmedsequences + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-demultiplexed-sequences: ARTIFACT SampleData[SequencesWithQuality] The single-end sequences to be trimmed. [required]" name="idemultiplexedsequences" optional="False" type="data"/> + <param label="--p-adapter: TEXT... Sequence of an adapter ligated to the 3' end. The List[Str] adapter and any subsequent bases are trimmed. If a `$` is appended, the adapter is only found if it is at the end of the read. If your sequence of interest is 'framed' by a 5' and a 3' adapter, use this parameter to define a 'linked' primer - see https://cutadapt.readthedocs.io for complete details. [optional]" name="padapter" optional="True" type="text"/> + <param label="--p-front: TEXT... Sequence of an adapter ligated to the 5' end. The List[Str] adapter and any preceding bases are trimmed. Partial matches at the 5' end are allowed. If a `^` character is prepended, the adapter is only found if it is at the beginning of the read. [optional]" name="pfront" optional="True" type="text"/> + <param label="--p-anywhere: TEXT... Sequence of an adapter that may be ligated to the 5' List[Str] or 3' end. Both types of matches as described under `adapter` and `front` are allowed. If the first base of the read is part of the match, the behavior is as with `front`, otherwise as with `adapter`. This option is mostly for rescuing failed library preparations - do not use if you know which end your adapter was ligated to. [optional]" name="panywhere" optional="True" type="text"/> + + <param label="--p-error-rate: PROPORTION Range(0, 1, inclusive_end=True) Maximum allowed error rate. [default: 0.1]" name="perrorrate" optional="True" type="float" min="0" max="1" exclude_max="False" value="0.1"/> + <param label="--p-no-indels: Do not allow insertions or deletions of bases when matching adapters. [default: False]" name="pnoindels" selected="False" type="boolean"/> + <param label="--p-times: INTEGER Remove multiple occurrences of an adapter if it is Range(1, None) repeated, up to `times` times. [default: 1]" name="ptimes" optional="True" type="integer" min="1" value="1"/> + <param label="--p-overlap: INTEGER Require at least `overlap` bases of overlap between Range(1, None) read and adapter for an adapter to be found. [default: 3]" name="poverlap" optional="True" type="integer" min="1" value="3"/> + <param label="--p-match-read-wildcards: --p-no-match-read-wildcards Interpret IUPAC wildcards (e.g., N) in reads. [default: False]" name="pmatchreadwildcards" selected="False" type="boolean"/> + <param label="--p-no-match-adapter-wildcards: Do not interpret IUPAC wildcards (e.g., N) in adapters. [default: False]" name="pnomatchadapterwildcards" selected="False" type="boolean"/> + <param label="--p-minimum-length: INTEGER Range(1, None) Discard reads shorter than specified value. Note, the cutadapt default of 0 has been overridden, because that value produces empty sequence records. [default: 1]" name="pminimumlength" optional="True" type="integer" min="1" value="1"/> + <param label="--p-discard-untrimmed: --p-no-discard-untrimmed Discard reads in which no adapter was found. [default: False]" name="pdiscarduntrimmed" selected="False" type="boolean"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: trimmedsequences.qza" name="otrimmedsequences"/> + </outputs> + <help><![CDATA[ +Find and remove adapters in demultiplexed single-end sequences. +############################################################### + +Search demultiplexed single-end sequences for adapters and remove them. The +parameter descriptions in this method are adapted from the official +cutadapt docs - please see those docs at https://cutadapt.readthedocs.io +for complete details. + +Parameters +---------- +demultiplexed_sequences : SampleData[SequencesWithQuality] + The single-end sequences to be trimmed. +adapter : List[Str], optional + Sequence of an adapter ligated to the 3' end. The adapter and any + subsequent bases are trimmed. If a `$` is appended, the adapter is only + found if it is at the end of the read. If your sequence of interest is + "framed" by a 5' and a 3' adapter, use this parameter to define a + "linked" primer - see https://cutadapt.readthedocs.io for complete + details. +front : List[Str], optional + Sequence of an adapter ligated to the 5' end. The adapter and any + preceding bases are trimmed. Partial matches at the 5' end are allowed. + If a `^` character is prepended, the adapter is only found if it is at + the beginning of the read. +anywhere : List[Str], optional + Sequence of an adapter that may be ligated to the 5' or 3' end. Both + types of matches as described under `adapter` and `front` are allowed. + If the first base of the read is part of the match, the behavior is as + with `front`, otherwise as with `adapter`. This option is mostly for + rescuing failed library preparations - do not use if you know which end + your adapter was ligated to. +error_rate : Float % Range(0, 1, inclusive_end=True), optional + Maximum allowed error rate. +indels : Bool, optional + Allow insertions or deletions of bases when matching adapters. +times : Int % Range(1, None), optional + Remove multiple occurrences of an adapter if it is repeated, up to + `times` times. +overlap : Int % Range(1, None), optional + Require at least `overlap` bases of overlap between read and adapter + for an adapter to be found. +match_read_wildcards : Bool, optional + Interpret IUPAC wildcards (e.g., N) in reads. +match_adapter_wildcards : Bool, optional + Interpret IUPAC wildcards (e.g., N) in adapters. +minimum_length : Int % Range(1, None), optional + Discard reads shorter than specified value. Note, the cutadapt default + of 0 has been overridden, because that value produces empty sequence + records. +discard_untrimmed : Bool, optional + Discard reads in which no adapter was found. + +Returns +------- +trimmed_sequences : SampleData[SequencesWithQuality] + The resulting trimmed sequences. + ]]></help> +<macros> + <import>qiime_citation.xml</import> +</macros> +<expand macro="qiime_citation"/> +</tool>