Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2/qiime_deblur_denoise-16S.xml @ 0:370e0b6e9826 draft
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author | florianbegusch |
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date | Wed, 17 Jul 2019 03:05:17 -0400 |
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children | de4c22a52df4 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_deblur_denoise-16S.xml Wed Jul 17 03:05:17 2019 -0400 @@ -0,0 +1,128 @@ +<?xml version="1.0" ?> +<tool id="qiime_deblur_denoise-16S" name="qiime deblur denoise-16S" version="2019.4"> + <description> - Deblur sequences using a 16S positive filter.</description> + <requirements> + <requirement type="package" version="2019.4">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime deblur denoise-16S + +--i-demultiplexed-seqs=$idemultiplexedseqs +--p-trim-length="$ptrimlength" + +#if $plefttrimlen: + --p-left-trim-len=$plefttrimlen +#end if + +#if $psamplestats: + --p-sample-stats +#end if + +#if $pmeanerror: + --p-mean-error=$pmeanerror +#end if + +#if $pindelprob: + --p-indel-prob=$pindelprob +#end if + +#if $pindelmax: + --p-indel-max=$pindelmax +#end if + +#if $pminreads: + --p-min-reads=$pminreads +#end if + +#if $pminsize: + --p-min-size=$pminsize +#end if + +#set $pjobstostart = '${GALAXY_SLOTS:-4}' + +#if str($pjobstostart): + --p-jobs-to-start="$pjobstostart" +#end if + + +#if $pnohashedfeatureids: + --p-no-hashed-feature-ids +#end if + +--o-table=otable +--o-representative-sequences=orepresentativesequences +--o-stats=ostats +; +cp otable.qza $otable; +cp orepresentativesequences.qza $orepresentativesequences; +cp ostats.qza $ostats + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-demultiplexed-seqs: ARTIFACT SampleData[SequencesWithQuality | PairedEndSequencesWithQuality | JoinedSequencesWithQuality] The demultiplexed sequences to be denoised. [required]" name="idemultiplexedseqs" optional="False" type="data"/> + <param label="--p-trim-length: INTEGER Sequence trim length, specify -1 to disable trimming. [required]" name="ptrimlength" optional="False" value="" type="integer"/> + <param label="--p-left-trim-len: INTEGER Range(0, None) Sequence trimming from the 5' end. A value of 0 will disable this trim. [default: 0]" name="plefttrimlen" optional="True" type="integer" min="0" value="0"/> + <param label="--p-sample-stats: --p-no-sample-stats If true, gather stats per sample. [default: False]" name="psamplestats" selected="False" type="boolean"/> + <param label="--p-mean-error: NUMBER The mean per nucleotide error, used for original sequence estimate. [default: 0.005]" name="pmeanerror" optional="True" type="float" value="0.005"/> + <param label="--p-indel-prob: NUMBER Insertion/deletion (indel) probability (same for N indels). [default: 0.01]" name="pindelprob" optional="True" type="float" value="0.01"/> + <param label="--p-indel-max: INTEGER Maximum number of insertion/deletions. [default: 3]" name="pindelmax" optional="True" type="integer" value="3"/> + <param label="--p-min-reads: INTEGER Retain only features appearing at least min-reads times across all samples in the resulting feature table. [default: 10]" name="pminreads" optional="True" type="integer" value="10"/> + <param label="--p-min-size: INTEGER In each sample, discard all features with an abundance less than min-size. [default: 2]" name="pminsize" optional="True" type="integer" value="2"/> + <param label="--p-no-hashed-feature-ids: If false, hash the feature IDs. [default: False]" name="pnohashedfeatureids" selected="False" type="boolean"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: table.qza" name="otable"/> + <data format="qza" label="${tool.name} on ${on_string}: representativesequences.qza" name="orepresentativesequences"/> + <data format="qza" label="${tool.name} on ${on_string}: stats.qza" name="ostats"/> + </outputs> + <help><![CDATA[ +Deblur sequences using a 16S positive filter. +############################################# + +Perform sequence quality control for Illumina data using the Deblur +workflow with a 16S reference as a positive filter. Only forward reads are +supported at this time. The specific reference used is the 88% OTUs from +Greengenes 13_8. This mode of operation should only be used when data were +generated from a 16S amplicon protocol on an Illumina platform. The +reference is only used to assess whether each sequence is likely to be 16S +by a local alignment using SortMeRNA with a permissive e-value; the +reference is not used to characterize the sequences. + +Parameters +---------- +demultiplexed_seqs : SampleData[SequencesWithQuality | PairedEndSequencesWithQuality | JoinedSequencesWithQuality] + The demultiplexed sequences to be denoised. +trim_length : Int + Sequence trim length, specify -1 to disable trimming. +left_trim_len : Int % Range(0, None), optional + Sequence trimming from the 5' end. A value of 0 will disable this trim. +sample_stats : Bool, optional + If true, gather stats per sample. +mean_error : Float, optional + The mean per nucleotide error, used for original sequence estimate. +indel_prob : Float, optional + Insertion/deletion (indel) probability (same for N indels). +indel_max : Int, optional + Maximum number of insertion/deletions. +min_reads : Int, optional + Retain only features appearing at least min_reads times across all + samples in the resulting feature table. +min_size : Int, optional + In each sample, discard all features with an abundance less than + min_size. +hashed_feature_ids : Bool, optional + If true, hash the feature IDs. + +Returns +------- +table : FeatureTable[Frequency] + The resulting denoised feature table. +representative_sequences : FeatureData[Sequence] + The resulting feature sequences. +stats : DeblurStats + Per-sample stats if requested. + ]]></help> +<macros> + <import>qiime_citation.xml</import> +</macros> +<expand macro="qiime_citation"/> +</tool>