Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2/qiime_demux_emp-paired.xml @ 0:370e0b6e9826 draft
Uploaded
author | florianbegusch |
---|---|
date | Wed, 17 Jul 2019 03:05:17 -0400 |
parents | |
children | 51025741f326 |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_demux_emp-paired.xml Wed Jul 17 03:05:17 2019 -0400 @@ -0,0 +1,92 @@ +<?xml version="1.0" ?> +<tool id="qiime_demux_emp-paired" name="qiime demux emp-paired" version="2019.4"> + <description> - Demultiplex paired-end sequence data generated with the EMP protocol.</description> + <requirements> + <requirement type="package" version="2019.4">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime demux emp-paired + +--i-seqs=$iseqs +--m-barcodes-column="$mbarcodescolumn" + + +#def list_dict_to_string(list_dict): +#set $file_list = list_dict[0]['additional_input'].__getattr__('file_name') +#for d in list_dict[1:]: + #set $file_list = $file_list + ',' + d['additional_input'].__getattr__('file_name') +#end for +#return $file_list +#end def + +--m-barcodes-file=$list_dict_to_string($input_files_mbarcodesfile) + + +#if $pnogolayerrorcorrection: + --p-no-golay-error-correction +#end if + +#if $prevcompbarcodes: + --p-rev-comp-barcodes +#end if + +#if $prevcompmappingbarcodes: + --p-rev-comp-mapping-barcodes +#end if + +--o-per-sample-sequences=opersamplesequences +--o-error-correction-details=oerrorcorrectiondetails +; +cp opersamplesequences.qza $opersamplesequences; +cp oerrorcorrectiondetails.qza $oerrorcorrectiondetails + ]]></command> + <inputs> + <repeat name="input_files_mbarcodesfile" optional="False" title="--m-barcodes-file"> + <param label="--m-barcodes-file: Metadata file or artifact viewable as metadata. This option may be supplied multiple times to merge metadata. [required]" name="additional_input" type="data" format="tabular,qza,no_unzip.zip" /> + </repeat> + <param format="qza,no_unzip.zip" label="--i-seqs: ARTIFACT EMPPairedEndSequences The paired-end sequences to be demultiplexed. [required]" name="iseqs" optional="False" type="data"/> + <param label="--m-barcodes-column: COLUMN MetadataColumn[Categorical] The sample metadata column containing the per-sample barcodes. [required]" name="mbarcodescolumn" optional="False" type="text"/> + <param label="--p-no-golay-error-correction: Do not perform 12nt Golay error correction on the barcode reads. [default: False]" name="pnogolayerrorcorrection" selected="False" type="boolean"/> + <param label="--p-rev-comp-barcodes: --p-no-rev-comp-barcodes If provided, the barcode sequence reads will be reverse complemented prior to demultiplexing. [default: False]" name="prevcompbarcodes" selected="False" type="boolean"/> + <param label="--p-rev-comp-mapping-barcodes: --p-no-rev-comp-mapping-barcodes If provided, the barcode sequences in the sample metadata will be reverse complemented prior to demultiplexing. [default: False]" name="prevcompmappingbarcodes" selected="False" type="boolean"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: persamplesequences.qza" name="opersamplesequences"/> + <data format="qza" label="${tool.name} on ${on_string}: errorcorrectiondetails.qza" name="oerrorcorrectiondetails"/> + </outputs> + <help><![CDATA[ +Demultiplex paired-end sequence data generated with the EMP protocol. +##################################################################### + +Demultiplex paired-end sequence data (i.e., map barcode reads to sample +ids) for data generated with the Earth Microbiome Project (EMP) amplicon +sequencing protocol. Details about this protocol can be found at +http://www.earthmicrobiome.org/protocols-and-standards/ + +Parameters +---------- +seqs : EMPPairedEndSequences + The paired-end sequences to be demultiplexed. +barcodes : MetadataColumn[Categorical] + The sample metadata column containing the per-sample barcodes. +golay_error_correction : Bool, optional + Perform 12nt Golay error correction on the barcode reads. +rev_comp_barcodes : Bool, optional + If provided, the barcode sequence reads will be reverse complemented + prior to demultiplexing. +rev_comp_mapping_barcodes : Bool, optional + If provided, the barcode sequences in the sample metadata will be + reverse complemented prior to demultiplexing. + +Returns +------- +per_sample_sequences : SampleData[PairedEndSequencesWithQuality] + The resulting demultiplexed sequences. +error_correction_details : ErrorCorrectionDetails + Detail about the barcode error corrections. + ]]></help> +<macros> + <import>qiime_citation.xml</import> +</macros> +<expand macro="qiime_citation"/> +</tool>