Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2/qiime_quality-control_evaluate-seqs.xml @ 29:3ba9833030c1 draft
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author | florianbegusch |
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date | Fri, 04 Sep 2020 13:12:49 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_quality-control_evaluate-seqs.xml Fri Sep 04 13:12:49 2020 +0000 @@ -0,0 +1,80 @@ +<?xml version="1.0" ?> +<tool id="qiime_quality-control_evaluate-seqs" name="qiime quality-control evaluate-seqs" + version="2020.8"> + <description>Compare query (observed) vs. reference (expected) sequences.</description> + <requirements> + <requirement type="package" version="2020.8">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime quality-control evaluate-seqs + +--i-query-sequences=$iquerysequences + +--i-reference-sequences=$ireferencesequences + +--o-visualization=ovisualization + +#if str($examples) != 'None': +--examples=$examples +#end if + +; +cp odatabase.qza $odatabase + +; +qiime tools export ovisualization.qzv --output-path out +&& mkdir -p '$ovisualization.files_path' +&& cp -r out/* '$ovisualization.files_path' +&& mv '$ovisualization.files_path/index.html' '$ovisualization' + +; +qiime tools export ovisualization.qzv --output-path out +&& mkdir -p '$ovisualization.files_path' +&& cp -r out/* '$ovisualization.files_path' +&& mv '$ovisualization.files_path/index.html' '$ovisualization' + + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-query-sequences: ARTIFACT FeatureData[Sequence] Sequences to test for exclusion [required]" name="iquerysequences" optional="False" type="data" /> + <param format="qza,no_unzip.zip" label="--i-reference-sequences: ARTIFACT FeatureData[Sequence] Reference sequences to align against feature sequences [required]" name="ireferencesequences" optional="False" type="data" /> + <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" /> + + </inputs> + + <outputs> + <data format="html" label="${tool.name} on ${on_string}: visualization.html" name="ovisualization" /> + + </outputs> + + <help><![CDATA[ +Compare query (observed) vs. reference (expected) sequences. +############################################################### + +This action aligns a set of query (e.g., observed) sequences against a set +of reference (e.g., expected) sequences to evaluate the quality of +alignment. The intended use is to align observed sequences against expected +sequences (e.g., from a mock community) to determine the frequency of +mismatches between observed sequences and the most similar expected +sequences, e.g., as a measure of sequencing/method error. However, any +sequences may be provided as input to generate a report on pairwise +alignment quality against a set of reference sequences. + +Parameters +---------- +query_sequences : FeatureData[Sequence] + Sequences to test for exclusion +reference_sequences : FeatureData[Sequence] + Reference sequences to align against feature sequences +show_alignments : Bool, optional + Option to plot pairwise alignments of query sequences and their top + hits. + +Returns +------- +visualization : Visualization + ]]></help> + <macros> + <import>qiime_citation.xml</import> + </macros> + <expand macro="qiime_citation"/> +</tool> \ No newline at end of file