Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2/qiime_cutadapt_trim-single.xml @ 14:a0a8d77a991c draft
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author | florianbegusch |
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date | Thu, 03 Sep 2020 09:51:29 +0000 |
parents | f190567fe3f6 |
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--- a/qiime2/qiime_cutadapt_trim-single.xml Thu Sep 03 09:46:00 2020 +0000 +++ b/qiime2/qiime_cutadapt_trim-single.xml Thu Sep 03 09:51:29 2020 +0000 @@ -1,77 +1,58 @@ <?xml version="1.0" ?> -<tool id="qiime_cutadapt_trim-single" name="qiime cutadapt trim-single" version="2019.7"> - <description> - Find and remove adapters in demultiplexed single-end sequences.</description> - <requirements> - <requirement type="package" version="2019.7">qiime2</requirement> - </requirements> - <command><![CDATA[ +<tool id="qiime_cutadapt_trim-single" name="qiime cutadapt trim-single" + version="2020.8"> + <description>Find and remove adapters in demultiplexed single-end sequences.</description> + <requirements> + <requirement type="package" version="2020.8">qiime2</requirement> + </requirements> + <command><![CDATA[ qiime cutadapt trim-single + --i-demultiplexed-sequences=$idemultiplexedsequences -#set $pcores = '${GALAXY_SLOTS:-4}' -#if str($pcores): --p-cores=$pcores -#end if - - -#if '__sq__' in str($padapter): - #set $padapter_temp = $padapter.replace('__sq__', "'") - #set $padapter = $padapter_temp -#end if -#if 'X' in str($padapter): - #set $padapter_temp = $padapter.replace('X', "$") - #set $padapter = $padapter_temp -#end if - -#if str($padapter): - --p-adapter="$padapter" -#end if - - - - - -#if '__sq__' in str($pfront): - #set $pfront_temp = $pfront.replace('__sq__', "'") - #set $pfront = $pfront_temp +#if $padapter: + --p-adapter #end if #if str($pfront): - --p-front="$pfront" + --p-front=$pfront +#end if +#if '__ob__' in str($panywhere): + #set $panywhere_temp = $panywhere.replace('__ob__', '[') + #set $panywhere = $panywhere_temp #end if - - - - - +#if '__cb__' in str($panywhere): + #set $panywhere_temp = $panywhere.replace('__cb__', ']') + #set $panywhere = $panywhere_temp +#end if +#if 'X' in str($panywhere): + #set $panywhere_temp = $panywhere.replace('X', '\\') + #set $panywhere = $panywhere_temp +#end if #if '__sq__' in str($panywhere): #set $panywhere_temp = $panywhere.replace('__sq__', "'") #set $panywhere = $panywhere_temp #end if - -#if str($panywhere): - --p-anywhere="$panywhere" +#if '__db__' in str($panywhere): + #set $panywhere_temp = $panywhere.replace('__db__', '"') + #set $panywhere = $panywhere_temp #end if - - +#if str($panywhere): + --p-anywhere=$panywhere +#end if -#if str($perrorrate): - --p-error-rate=$perrorrate -#end if +--p-error-rate=$perrorrate #if $pnoindels: --p-no-indels #end if -#if str($ptimes): - --p-times=$ptimes -#end if +--p-times=$ptimes -#if str($poverlap): - --p-overlap=$poverlap -#end if +--p-overlap=$poverlap #if $pmatchreadwildcards: --p-match-read-wildcards @@ -81,9 +62,7 @@ --p-no-match-adapter-wildcards #end if -#if str($pminimumlength): - --p-minimum-length=$pminimumlength -#end if +--p-minimum-length=$pminimumlength #if $pdiscarduntrimmed: --p-discard-untrimmed @@ -91,28 +70,37 @@ --o-trimmed-sequences=otrimmedsequences +#if str($examples) != 'None': +--examples=$examples +#end if + ; cp otrimmedsequences.qza $otrimmedsequences - ]]></command> - <inputs> - <param format="qza,no_unzip.zip" label="--i-demultiplexed-sequences: ARTIFACT SampleData[SequencesWithQuality] The single-end sequences to be trimmed. [required]" name="idemultiplexedsequences" optional="False" type="data"/> - <param label="--p-adapter: TEXT... Sequence of an adapter ligated to the 3' end. The List[Str] adapter and any subsequent bases are trimmed. If a `$` is appended, the adapter is only found if it is at the end of the read. If your sequence of interest is 'framed' by a 5' and a 3' adapter, use this parameter to define a 'linked' primer - see https://cutadapt.readthedocs.io for complete details. [optional]" name="padapter" optional="True" type="text"/> - <param label="--p-front: TEXT... Sequence of an adapter ligated to the 5' end. The List[Str] adapter and any preceding bases are trimmed. Partial matches at the 5' end are allowed. If a `^` character is prepended, the adapter is only found if it is at the beginning of the read. [optional]" name="pfront" optional="True" type="text"/> - <param label="--p-anywhere: TEXT... Sequence of an adapter that may be ligated to the 5' List[Str] or 3' end. Both types of matches as described under `adapter` and `front` are allowed. If the first base of the read is part of the match, the behavior is as with `front`, otherwise as with `adapter`. This option is mostly for rescuing failed library preparations - do not use if you know which end your adapter was ligated to. [optional]" name="panywhere" optional="True" type="text"/> - <param label="--p-error-rate: PROPORTION Range(0, 1, inclusive_end=True) Maximum allowed error rate. [default: 0.1]" name="perrorrate" optional="True" type="float" min="0" max="1" exclude_max="False" value="0.1"/> - <param label="--p-no-indels: Do not allow insertions or deletions of bases when matching adapters. [default: False]" name="pnoindels" selected="False" type="boolean"/> - <param label="--p-times: INTEGER Remove multiple occurrences of an adapter if it is Range(1, None) repeated, up to `times` times. [default: 1]" name="ptimes" optional="True" type="integer" min="1" value="1"/> - <param label="--p-overlap: INTEGER Require at least `overlap` bases of overlap between Range(1, None) read and adapter for an adapter to be found. [default: 3]" name="poverlap" optional="True" type="integer" min="1" value="3"/> - <param label="--p-match-read-wildcards: --p-no-match-read-wildcards Interpret IUPAC wildcards (e.g., N) in reads. [default: False]" name="pmatchreadwildcards" selected="False" type="boolean"/> - <param label="--p-no-match-adapter-wildcards: Do not interpret IUPAC wildcards (e.g., N) in adapters. [default: False]" name="pnomatchadapterwildcards" selected="False" type="boolean"/> - <param label="--p-minimum-length: INTEGER Range(1, None) Discard reads shorter than specified value. Note, the cutadapt default of 0 has been overridden, because that value produces empty sequence records. [default: 1]" name="pminimumlength" optional="True" type="integer" min="1" value="1"/> - <param label="--p-discard-untrimmed: --p-no-discard-untrimmed Discard reads in which no adapter was found. [default: False]" name="pdiscarduntrimmed" selected="False" type="boolean"/> - </inputs> - <outputs> - <data format="qza" label="${tool.name} on ${on_string}: trimmedsequences.qza" name="otrimmedsequences"/> - </outputs> - <help><![CDATA[ + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-demultiplexed-sequences: ARTIFACT SampleData[SequencesWithQuality] The single-end sequences to be trimmed. [required]" name="idemultiplexedsequences" optional="False" type="data" /> + <param label="--p-adapter: --p-adapter: TEXT... Sequence of an adapter ligated to the 3\' end. The List[Str] adapter and any subsequent bases are trimmed. If a `$` is appended, the adapter is only found if it is at the end of the read. If your sequence of interest is \'framed\' by a 5\' and a 3\' adapter, use this parameter to define a \'linked\' primer - see https://cutadapt.readthedocs.io for complete details. [optional]" name="padapter" selected="False" type="boolean" /> + <param label="--p-front: TEXT... Sequence of an adapter ligated to the 5\' end. The List[Str] adapter and any preceding bases are trimmed. Partial matches at the 5\' end are allowed. If a `^` character is prepended, the adapter is only found if it is at the beginning of the read. [optional]" name="pfront" optional="False" type="text" /> + <param label="--p-anywhere: TEXT... Sequence of an adapter that may be ligated to the 5\' List[Str] or 3\' end. Both types of matches as described under `adapter` and `front` are allowed. If the first base of the read is part of the match, the behavior is as with `front`, otherwise as with `adapter`. This option is mostly for rescuing failed library preparations - do not use if you know which end your adapter was ligated to. [optional]" name="panywhere" optional="False" type="text" /> + <param exclude_max="False" label="--p-error-rate: PROPORTION Range(0, 1, inclusive_end=True) Maximum allowed error rate. [default: 0.1]" max="1" min="0" name="perrorrate" optional="True" type="float" value="0.1" /> + <param label="--p-no-indels: Do not allow insertions or deletions of bases when matching adapters. [default: True]" name="pnoindels" selected="False" type="boolean" /> + <param label="--p-times: INTEGER Remove multiple occurrences of an adapter if it is Range(1, None) repeated, up to `times` times. [default: 1]" min="1" name="ptimes" optional="True" type="integer" value="1" /> + <param label="--p-overlap: INTEGER Require at least `overlap` bases of overlap between Range(1, None) read and adapter for an adapter to be found. [default: 3]" min="1" name="poverlap" optional="True" type="integer" value="3" /> + <param label="--p-match-read-wildcards: --p-match-read-wildcards: / --p-no-match-read-wildcards Interpret IUPAC wildcards (e.g., N) in reads. [default: False]" name="pmatchreadwildcards" selected="False" type="boolean" /> + <param label="--p-no-match-adapter-wildcards: Do not interpret IUPAC wildcards (e.g., N) in adapters. [default: True]" name="pnomatchadapterwildcards" selected="False" type="boolean" /> + <param label="--p-minimum-length: INTEGER Range(1, None) Discard reads shorter than specified value. Note, the cutadapt default of 0 has been overridden, because that value produces empty sequence records. [default: 1]" min="1" name="pminimumlength" optional="True" type="integer" value="1" /> + <param label="--p-discard-untrimmed: --p-discard-untrimmed: / --p-no-discard-untrimmed Discard reads in which no adapter was found. [default: False]" name="pdiscarduntrimmed" selected="False" type="boolean" /> + <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" /> + + </inputs> + + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: trimmedsequences.qza" name="otrimmedsequences" /> + + </outputs> + + <help><![CDATA[ Find and remove adapters in demultiplexed single-end sequences. ############################################################### @@ -125,6 +113,8 @@ ---------- demultiplexed_sequences : SampleData[SequencesWithQuality] The single-end sequences to be trimmed. +cores : Int % Range(1, None), optional + Number of CPU cores to use. adapter : List[Str], optional Sequence of an adapter ligated to the 3' end. The adapter and any subsequent bases are trimmed. If a `$` is appended, the adapter is only @@ -169,9 +159,9 @@ ------- trimmed_sequences : SampleData[SequencesWithQuality] The resulting trimmed sequences. - ]]></help> -<macros> + ]]></help> + <macros> <import>qiime_citation.xml</import> -</macros> -<expand macro="qiime_citation"/> -</tool> + </macros> + <expand macro="qiime_citation"/> +</tool> \ No newline at end of file