Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2-2020.8/qiime_vsearch_uchime-ref.xml @ 27:a917731ff917 draft
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author | florianbegusch |
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date | Fri, 04 Sep 2020 12:59:12 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2-2020.8/qiime_vsearch_uchime-ref.xml Fri Sep 04 12:59:12 2020 +0000 @@ -0,0 +1,110 @@ +<?xml version="1.0" ?> +<tool id="qiime_vsearch_uchime-ref" name="qiime vsearch uchime-ref" + version="2020.8"> + <description>Reference-based chimera filtering with vsearch.</description> + <requirements> + <requirement type="package" version="2020.8">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime vsearch uchime-ref + +--i-sequences=$isequences + +--i-table=$itable + +--i-reference-sequences=$ireferencesequences + +--p-dn=$pdn + +--p-mindiffs=$pmindiffs + +--p-mindiv=$pmindiv + +--p-minh=$pminh + +--p-xn=$pxn + +--p-threads=$pthreads + +--o-chimeras=ochimeras + +--o-nonchimeras=ononchimeras + +--o-stats=ostats + +#if str($examples) != 'None': +--examples=$examples +#end if + +; +cp ostats.qza $ostats + + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-sequences: ARTIFACT FeatureData[Sequence] The feature sequences to be chimera-checked. [required]" name="isequences" optional="False" type="data" /> + <param format="qza,no_unzip.zip" label="--i-table: ARTIFACT FeatureTable[Frequency] Feature table (used for computing total feature abundances). [required]" name="itable" optional="False" type="data" /> + <param format="qza,no_unzip.zip" label="--i-reference-sequences: ARTIFACT FeatureData[Sequence] The non-chimeric reference sequences. [required]" name="ireferencesequences" optional="False" type="data" /> + <param label="--p-dn: NUMBER No vote pseudo-count, corresponding to the Range(0.0, None) parameter n in the chimera scoring function. [default: 1.4]" min="0.0" name="pdn" optional="True" type="float" value="1.4" /> + <param label="--p-mindiffs: INTEGER Minimum number of differences per segment. Range(1, None) [default: 3]" min="1" name="pmindiffs" optional="True" type="integer" value="3" /> + <param label="--p-mindiv: NUMBER Minimum divergence from closest parent. Range(0.0, None) [default: 0.8]" min="0.0" name="pmindiv" optional="True" type="float" value="0.8" /> + <param exclude_max="False" label="--p-minh: PROPORTION Range(0.0, 1.0, inclusive_end=True) Minimum score (h). Increasing this value tends to reduce the number of false positives and to decrease sensitivity. [default: 0.28]" max="1.0" min="0.0" name="pminh" optional="True" type="float" value="0.28" /> + <param exclude_min="True" label="--p-xn: NUMBER Range(1.0, None, inclusive_start=False) No vote weight, corresponding to the parameter beta in the scoring function. [default: 8.0]" max="20.0" min="1.0" name="pxn" optional="True" type="float" value="8.0" /> + <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" /> + + </inputs> + + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: chimeras.qza" name="ochimeras" /> + <data format="qza" label="${tool.name} on ${on_string}: nonchimeras.qza" name="ononchimeras" /> + <data format="qza" label="${tool.name} on ${on_string}: stats.qza" name="ostats" /> + + </outputs> + + <help><![CDATA[ +Reference-based chimera filtering with vsearch. +############################################################### + +Apply the vsearch uchime_ref method to identify chimeric feature sequences. +The results of this method can be used to filter chimeric features from the +corresponding feature table. For additional details, please refer to the +vsearch documentation. + +Parameters +---------- +sequences : FeatureData[Sequence] + The feature sequences to be chimera-checked. +table : FeatureTable[Frequency] + Feature table (used for computing total feature abundances). +reference_sequences : FeatureData[Sequence] + The non-chimeric reference sequences. +dn : Float % Range(0.0, None), optional + No vote pseudo-count, corresponding to the parameter n in the chimera + scoring function. +mindiffs : Int % Range(1, None), optional + Minimum number of differences per segment. +mindiv : Float % Range(0.0, None), optional + Minimum divergence from closest parent. +minh : Float % Range(0.0, 1.0, inclusive_end=True), optional + Minimum score (h). Increasing this value tends to reduce the number of + false positives and to decrease sensitivity. +xn : Float % Range(1.0, None, inclusive_start=False), optional + No vote weight, corresponding to the parameter beta in the scoring + function. +threads : Int % Range(0, 256, inclusive_end=True), optional + The number of threads to use for computation. Passing 0 will launch one + thread per CPU core. + +Returns +------- +chimeras : FeatureData[Sequence] + The chimeric sequences. +nonchimeras : FeatureData[Sequence] + The non-chimeric sequences. +stats : UchimeStats + Summary statistics from chimera checking. + ]]></help> + <macros> + <import>qiime_citation.xml</import> + </macros> + <expand macro="qiime_citation"/> +</tool> \ No newline at end of file