Mercurial > repos > florianbegusch > qiime2_suite
diff qiime2-2020.8/qiime_demux_emp-single.xml @ 20:d93d8888f0b0 draft
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author | florianbegusch |
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date | Fri, 04 Sep 2020 12:44:24 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2-2020.8/qiime_demux_emp-single.xml Fri Sep 04 12:44:24 2020 +0000 @@ -0,0 +1,125 @@ +<?xml version="1.0" ?> +<tool id="qiime_demux_emp-single" name="qiime demux emp-single" + version="2020.8"> + <description>Demultiplex sequence data generated with the EMP protocol.</description> + <requirements> + <requirement type="package" version="2020.8">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime demux emp-single + +--i-seqs=$iseqs +# if $input_files_mbarcodesfile: + # def list_dict_to_string(list_dict): + # set $file_list = list_dict[0]['additional_input'].__getattr__('file_name') + # for d in list_dict[1:]: + # set $file_list = $file_list + ' --m-barcodes-file=' + d['additional_input'].__getattr__('file_name') + # end for + # return $file_list + # end def +--m-barcodes-file=$list_dict_to_string($input_files_mbarcodesfile) +# end if + +#if '__ob__' in str($mbarcodescolumn): + #set $mbarcodescolumn_temp = $mbarcodescolumn.replace('__ob__', '[') + #set $mbarcodescolumn = $mbarcodescolumn_temp +#end if +#if '__cb__' in str($mbarcodescolumn): + #set $mbarcodescolumn_temp = $mbarcodescolumn.replace('__cb__', ']') + #set $mbarcodescolumn = $mbarcodescolumn_temp +#end if +#if 'X' in str($mbarcodescolumn): + #set $mbarcodescolumn_temp = $mbarcodescolumn.replace('X', '\\') + #set $mbarcodescolumn = $mbarcodescolumn_temp +#end if +#if '__sq__' in str($mbarcodescolumn): + #set $mbarcodescolumn_temp = $mbarcodescolumn.replace('__sq__', "'") + #set $mbarcodescolumn = $mbarcodescolumn_temp +#end if +#if '__db__' in str($mbarcodescolumn): + #set $mbarcodescolumn_temp = $mbarcodescolumn.replace('__db__', '"') + #set $mbarcodescolumn = $mbarcodescolumn_temp +#end if + +--m-barcodes-column=$mbarcodescolumn + + +#if $pnogolayerrorcorrection: + --p-no-golay-error-correction +#end if + +#if $prevcompbarcodes: + --p-rev-comp-barcodes +#end if + +#if $prevcompmappingbarcodes: + --p-rev-comp-mapping-barcodes +#end if + +--o-per-sample-sequences=opersamplesequences + +--o-error-correction-details=oerrorcorrectiondetails + +#if str($examples) != 'None': +--examples=$examples +#end if + +; +cp oerrorcorrectiondetails.qza $oerrorcorrectiondetails + + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-seqs: ARTIFACT RawSequences | EMPSingleEndSequences | EMPPairedEndSequences The single-end sequences to be demultiplexed. [required]" name="iseqs" optional="False" type="data" /> + <repeat name="input_files_mbarcodesfile" optional="True" title="--m-barcodes-file"> + <param format="tabular,qza,no_unzip.zip" label="--m-barcodes-file: METADATA" name="additional_input" optional="True" type="data" /> + </repeat> + <param label="--m-barcodes-column: COLUMN MetadataColumn[Categorical] The sample metadata column containing the per-sample barcodes. [required]" name="mbarcodescolumn" optional="False" type="text" /> + <param label="--p-no-golay-error-correction: Do not perform 12nt Golay error correction on the barcode reads. [default: True]" name="pnogolayerrorcorrection" selected="False" type="boolean" /> + <param format="tabular,qza,no_unzip.zip" label="--p-rev-comp-barcodes: --p-rev-comp-barcodes: / --p-no-rev-comp-barcodes If provided, the barcode sequence reads will be reverse complemented prior to demultiplexing. [default: False]" name="prevcompbarcodes" selected="False" type="boolean" /> + <param format="tabular,qza,no_unzip.zip" label="--p-rev-comp-mapping-barcodes: --p-rev-comp-mapping-barcodes: / --p-no-rev-comp-mapping-barcodes If provided, the barcode sequences in the sample metadata will be reverse complemented prior to demultiplexing. [default: False]" name="prevcompmappingbarcodes" selected="False" type="boolean" /> + <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" /> + + </inputs> + + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: persamplesequences.qza" name="opersamplesequences" /> + <data format="qza" label="${tool.name} on ${on_string}: errorcorrectiondetails.qza" name="oerrorcorrectiondetails" /> + + </outputs> + + <help><![CDATA[ +Demultiplex sequence data generated with the EMP protocol. +############################################################### + +Demultiplex sequence data (i.e., map barcode reads to sample ids) for data +generated with the Earth Microbiome Project (EMP) amplicon sequencing +protocol. Details about this protocol can be found at +http://www.earthmicrobiome.org/protocols-and-standards/ + +Parameters +---------- +seqs : RawSequences | EMPSingleEndSequences | EMPPairedEndSequences + The single-end sequences to be demultiplexed. +barcodes : MetadataColumn[Categorical] + The sample metadata column containing the per-sample barcodes. +golay_error_correction : Bool, optional + Perform 12nt Golay error correction on the barcode reads. +rev_comp_barcodes : Bool, optional + If provided, the barcode sequence reads will be reverse complemented + prior to demultiplexing. +rev_comp_mapping_barcodes : Bool, optional + If provided, the barcode sequences in the sample metadata will be + reverse complemented prior to demultiplexing. + +Returns +------- +per_sample_sequences : SampleData[SequencesWithQuality] + The resulting demultiplexed sequences. +error_correction_details : ErrorCorrectionDetails + Detail about the barcode error corrections. + ]]></help> + <macros> + <import>qiime_citation.xml</import> + </macros> + <expand macro="qiime_citation"/> +</tool> \ No newline at end of file