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view qiime2/qiime_feature-classifier_classify-consensus-vsearch.xml @ 7:85b520815e85 draft
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author | florianbegusch |
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date | Tue, 13 Aug 2019 07:53:59 -0400 |
parents | de4c22a52df4 |
children | f190567fe3f6 |
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<?xml version="1.0" ?> <tool id="qiime_feature-classifier_classify-consensus-vsearch" name="qiime feature-classifier classify-consensus-vsearch" version="2019.4"> <description> - VSEARCH consensus taxonomy classifier</description> <requirements> <requirement type="package" version="2019.4">qiime2</requirement> </requirements> <command><![CDATA[ qiime feature-classifier classify-consensus-vsearch --i-query=$iquery --i-reference-reads=$ireferencereads #if str( $id_to_taxonomy_fp.selector ) == 'history' #set $tax = $id_to_taxonomy_fp.taxonomy_fp --i-reference-taxonomy '$tax' #else: #set $tax = $id_to_taxonomy_fp.taxonomy_fp.fields.path --i-reference-taxonomy '$tax' #end if #if str($pmaxaccepts): --p-maxaccepts=$pmaxaccepts #end if #if str($ppercidentity): --p-perc-identity=$ppercidentity #end if #if str($pquerycov): --p-query-cov=$pquerycov #end if #if str($pstrand) != 'None': --p-strand=$pstrand #end if #if str($pminconsensus): --p-min-consensus=$pminconsensus #end if #if '__sq__' in str($punassignablelabel): #set $punassignablelabel_temp = $punassignablelabel.replace('__sq__', "'") #set $punassignablelabel = $punassignablelabel_temp #end if #if str($punassignablelabel): --p-unassignable-label="$punassignablelabel" #end if #set $pthreads = '${GALAXY_SLOTS:-4}' #if str($pthreads): --p-threads="$pthreads" #end if --o-classification=oclassification ; cp oclassification.qza $oclassification ]]></command> <inputs> <param format="qza,no_unzip.zip" label="--i-query: ARTIFACT FeatureData[Sequence] Sequences to classify taxonomically. [required]" name="iquery" optional="False" type="data"/> <param format="qza,no_unzip.zip" label="--i-reference-reads: ARTIFACT FeatureData[Sequence] reference sequences. [required]" name="ireferencereads" optional="False" type="data"/> <conditional name="id_to_taxonomy_fp" optional="True"> <param name="selector" type="select" label="Reference taxonomy to query"> <option value="cached">Public databases</option> <option value="history">Databases from your history</option> </param> <when value="cached"> <param argument="--taxonomy_fp" label="Reference taxonomy" type="select" optional="True"> <options from_data_table="qiime_taxonomy" /> </param> </when> <when value="history"> <param argument="--taxonomy_fp" type="data" format="qza,no_unzip.zip" label="Reference databases" optional="True" /> </when> </conditional> <param label="--p-maxaccepts: INTEGER Maximum number of hits to keep for each query. Set Range(0, None) to 0 to keep all hits > perc-identity similarity. Must be in range [0, infinity]. [default: 10]" name="pmaxaccepts" optional="True" type="integer" min="0" value="10"/> <param label="--p-perc-identity: PROPORTION Range(0.0, 1.0, inclusive_end=True) Reject match if percent identity to query is lower. Must be in range [0.0, 1.0]. [default: 0.8]" name="ppercidentity" optional="True" type="float" min="0" max="1" exclude_max="False" value="0.8"/> <param label="--p-query-cov: PROPORTION Range(0.0, 1.0, inclusive_end=True) Reject match if query alignment coverage per high-scoring pair is lower. Must be in range [0.0, 1.0]. [default: 0.8]" name="pquerycov" optional="True" type="float" min="0" max="1" exclude_max="False" value="0.8"/> <param label="--p-strand: Align against reference sequences in forward ('plus') or both directions ('both'). [default: 'both']" name="pstrand" optional="True" type="select"> <option value="None">Selection is Optional</option> <option selected="True" value="both">both</option> <option value="plus">plus</option> </param> <param label="--p-min-consensus: NUMBER Range(0.5, 1.0, inclusive_start=False, inclusive_end=True) Minimum fraction of assignments must match top hit to be accepted as consensus assignment. Must be in range (0.5, 1.0]. [default: 0.51]" name="pminconsensus" optional="True" type="float" min="0.5" max="1" exclude_min="True" exclude_max="False" value="0.51"/> <param label="--p-unassignable-label: TEXT [default: 'Unassigned']" name="punassignablelabel" optional="True" type="text" value="Unassigned"/> </inputs> <outputs> <data format="qza" label="${tool.name} on ${on_string}: classification.qza" name="oclassification"/> </outputs> <help><![CDATA[ VSEARCH consensus taxonomy classifier ##################################### Assign taxonomy to query sequences using VSEARCH. Performs VSEARCH global alignment between query and reference_reads, then assigns consensus taxonomy to each query sequence from among maxaccepts top hits, min_consensus of which share that taxonomic assignment. Unlike classify- consensus-blast, this method searches the entire reference database before choosing the top N hits, not the first N hits. Parameters ---------- query : FeatureData[Sequence] Sequences to classify taxonomically. reference_reads : FeatureData[Sequence] reference sequences. reference_taxonomy : FeatureData[Taxonomy] reference taxonomy labels. maxaccepts : Int % Range(0, None), optional Maximum number of hits to keep for each query. Set to 0 to keep all hits > perc_identity similarity. Must be in range [0, infinity]. perc_identity : Float % Range(0.0, 1.0, inclusive_end=True), optional Reject match if percent identity to query is lower. Must be in range [0.0, 1.0]. query_cov : Float % Range(0.0, 1.0, inclusive_end=True), optional Reject match if query alignment coverage per high-scoring pair is lower. Must be in range [0.0, 1.0]. strand : Str % Choices('both', 'plus'), optional Align against reference sequences in forward ("plus") or both directions ("both"). min_consensus : Float % Range(0.5, 1.0, inclusive_start=False, inclusive_end=True), optional Minimum fraction of assignments must match top hit to be accepted as consensus assignment. Must be in range (0.5, 1.0]. unassignable_label : Str, optional \ Returns ------- classification : FeatureData[Taxonomy] The resulting taxonomy classifications. ]]></help> <macros> <import>qiime_citation.xml</import> </macros> <expand macro="qiime_citation"/> </tool>