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author | florianbegusch |
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date | Fri, 04 Sep 2020 12:46:48 +0000 |
parents | d93d8888f0b0 |
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<?xml version="1.0" ?> <tool id="qiime_tools_import" name="qiime tools import" version="2020.8"> <description> - Import data into a new QIIME 2 Artifact.</description> <requirements> <requirement type="package" version="2020.8">qiime2</requirement> </requirements> <command> <![CDATA[ mkdir input; #set $in_= 'input' #if '__ob__' in str($semantic_type): #set $type_temp = str($semantic_type).replace('__ob__', '[') #set $type_temp = str($type_temp).replace('__cb__', ']') #set $semantic_type = $type_temp #end if #def is_fastq($f_test) #set $f = open($f_test, 'rb') #set $fastq = str($f.readline()[0]) #if str($f.readline()[0]) == '@' #$f.close() #return True #end if #$f.close() #return $fastq #end def ## Symlinks creation or On the fly creation of a combo file #if $input_type.type == 'list_list' ## Symlinks creation or On the fly creation of a combo file #for $f in $input_type.list_list: #set $is_fastq_file = $is_fastq(str($f)) #if $is_fastq_file: #set $file_for_processing = "'input/" + $f.name + '.gz' + "'" gzip -c ${f} > $file_for_processing; ##echo $is_fastq_file; #else #set $file_for_processing = "'input/" + $f.name + "'" ln -s ${f} $file_for_processing; ##echo $is_fastq_file; #end if #end for #if 'SingleEndFastqManifestPhred' in str($inputformat): #set $cwf = str($outputpath.extra_files_path) + "_manifest_file.txt" #set $tt = str("sample-id,absolute-filepath,direction") echo $tt >> $cwf; #for $f in $input_type.list_list: #set $tt = str($f.name).split("_")[0]+",$PWD/input/"+str($f.name).split(".")[0]+".fastq.gz,forward\n" echo $tt >> $cwf; #end for #set $in_= str($cwf) #end if #elif $input_type.type == 'list_paired_collection': #set $cwf = str($outputpath.extra_files_path) + "_manifest_file.txt" #set $tt = str("sample-id,absolute-filepath,direction") echo $tt >> $cwf; #for $pair in $input_type.list_paired_collection: #set $forward_is_fastq_file = $is_fastq(str($pair.forward)) #set $reverse_is_fastq_file = $is_fastq(str($pair.reverse)) #if $forward_is_fastq_file: gzip -c ${pair.forward} > input/${pair.name}_R1_001.fastq.gz; #set $tt = str($pair.name).split("_")[0]+",$PWD/input/"+str($pair.name)+"_R1_001.fastq.gz,forward\n" echo $tt >> $cwf; #else ln -s ${pair.forward} input/${pair.name}_R1_001.fastq.gz; #set $tt = str($pair.name).split("_")[0]+",$PWD/input/"+str($pair.name)+"_R1_001.fastq.gz,forward\n" echo $tt >> $cwf; #end if #if $reverse_is_fastq_file: gzip -c ${pair.reverse} > input/${pair.name}_R2_001.fastq.gz; #set $tt = str($pair.name).split("_")[0]+",$PWD/input/"+str($pair.name)+"_R2_001.fastq.gz,reverse\n" echo $tt >> $cwf; #else ln -s ${pair.reverse} input/${pair.name}_R2_001.fastq.gz; #set $tt = str($pair.name).split("_")[0]+",$PWD/input/"+str($pair.name)+"_R2_001.fastq.gz,reverse\n" echo $tt >> $cwf; #end if #end for #if 'PairedEndFastqManifestPhred' in str($inputformat) or 'SingleEndFastqManifestPhred33V2' in str($inputformat) or 'SingleEndFastqManifestPhred33' in str($inputformat): #set $in_= str($cwf) #elif 'Casava' in str($inputformat): #set $in_= 'input' #end if #else #set $res = $is_fastq(str($file)) #if $res gzip -c ${file} > ${file.name}.gz; #set $in_= $file.name + '.gz' #else #set $in_= str($file) #end if #if "MultiplexedSingleEndBarcodeInSequence" in str($semantic_type): mv *_R1_* forward.fastq.gz; #set $in_= 'forward.fastq.gz'; #end if #end if ## rename files to predefined names for "MultiplexedPairedEndBarcodeInSequence" #if "MultiplexedPairedEndBarcodeInSequence" in str($semantic_type): mv input/*_R1_* input/forward.fastq.gz; mv input/*_R2_* input/reverse.fastq.gz; #end if qiime tools import --input-path=$in_ #if str($inputformat) != 'None': #if '__ob__' in str($inputformat): #set $inputformat_temp = str($inputformat).replace('__ob__', '[') #set $inputformat_temp = str($inputformat_temp).replace('__cb__', ']') #set $inputformat = $inputformat_temp #end if --input-format="$inputformat" #end if --type="$semantic_type" --output-path=outputpath.qza ; cp outputpath.qza $outputpath ]]> </command> <inputs> <conditional name="input_type"> <param name="type" type="select" label="Select a way to provide data, either using a file or a list" help=""> <option value="regular">Input file</option> <option selected="true" value="list_paired_collection">Multiple pairs - Combo mode (list:paired collection)</option> <option value="list_list">Input list</option> </param> <when value="regular"> <param name="file" type="data" multiple="false" label="Input file" /> </when> <when value="list_paired_collection"> <param name="list_paired_collection" type="data_collection" collection_type="list:paired" label="Fastq pairs (collection)" help="Dataset collection made from multiple pairs of files" /> </when> <when value="list_list"> <param name="list_list" type="data_collection" collection_type="list" label="Input list" /> </when> </conditional> <param label="--type: The semantic type of the artifact that will be created upon importing. [required]" name="semantic_type" optional="False" type="select"> <option value="DeblurStats">DeblurStats</option> <option value="DistanceMatrix">DistanceMatrix</option> <option value="EMPPairedEndSequences">EMPPairedEndSequences</option> <option value="EMPSingleEndSequences">EMPSingleEndSequences</option> <option value="ErrorCorrectionDetails">ErrorCorrectionDetails</option> <option value="FeatureData[AlignedSequence]">FeatureData[AlignedSequence]</option> <option value="FeatureData[Importance]">FeatureData[Importance]</option> <option value="FeatureData[PairedEndSequence]">FeatureData[PairedEndSequence]</option> <option value="FeatureData[Sequence]">FeatureData[Sequence]</option> <option value="FeatureData[Taxonomy]">FeatureData[Taxonomy]</option> <option value="FeatureTable[Balance]">FeatureTable[Balance]</option> <option value="FeatureTable[Composition]">FeatureTable[Composition]</option> <option value="FeatureTable[Frequency]">FeatureTable[Frequency]</option> <option value="FeatureTable[PercentileNormalized]">FeatureTable[PercentileNormalized]</option> <option value="FeatureTable[PresenceAbsence]">FeatureTable[PresenceAbsence]</option> <option value="FeatureTable[RelativeFrequency]">FeatureTable[RelativeFrequency]</option> <option value="Hierarchy">Hierarchy</option> <option value="MultiplexedPairedEndBarcodeInSequence">MultiplexedPairedEndBarcodeInSequence</option> <option value="MultiplexedSingleEndBarcodeInSequence">MultiplexedSingleEndBarcodeInSequence</option> <option value="PCoAResults">PCoAResults</option> <option value="Phylogeny[Rooted]">Phylogeny[Rooted]</option> <option value="Phylogeny[Unrooted]">Phylogeny[Unrooted]</option> <option value="Placements">Placements</option> <option value="QualityFilterStats">QualityFilterStats</option> <option value="RawSequences">RawSequences</option> <option value="SampleData[AlphaDiversity]">SampleData[AlphaDiversity]</option> <option value="SampleData[BooleanSeries]">SampleData[BooleanSeries]</option> <option value="SampleData[ClassifierPredictions]">SampleData[ClassifierPredictions]</option> <option value="SampleData[DADA2Stats]">SampleData[DADA2Stats]</option> <option value="SampleData[FirstDifferences]">SampleData[FirstDifferences]</option> <option value="SampleData[JoinedSequencesWithQuality]">SampleData[JoinedSequencesWithQuality]</option> <option selected="True" value="SampleData[PairedEndSequencesWithQuality]">SampleData[PairedEndSequencesWithQuality]</option> <option value="SampleData[RegressorPredictions]">SampleData[RegressorPredictions]</option> <option value="SampleData[SequencesWithQuality]">SampleData[SequencesWithQuality]</option> <option value="SampleData[Sequences]">SampleData[Sequences]</option> <option value="SampleData[Classifier]">SampleData[Classifier]</option> <option value="SampleData[Regressor]">SampleData[Regressor]</option> <option value="TaxonomicClassifier">TaxonomicClassifier</option> <option value="UchimeStats">UchimeStats</option> </param> <param label="--input-format: The format of the data to be imported. If not provided, data must be in the format expected by the semantic type provided via --type." name="inputformat" optional="True" type="select"> <option value="AlignedDNAFASTAFormat">AlignedDNAFASTAFormat</option> <option value="AlignedDNASequencesDirectoryFormat">AlignedDNASequencesDirectoryFormat</option> <option value="AlphaDiversityDirectoryFormat">AlphaDiversityDirectoryFormat</option> <option value="AlphaDiversityFormat">AlphaDiversityFormat</option> <option value="BIOMV100DirFmt">BIOMV100DirFmt</option> <option value="BIOMV100Format">BIOMV100Format</option> <option value="BIOMV210DirFmt">BIOMV210DirFmt</option> <option value="BIOMV210Format">BIOMV210Format</option> <option value="BooleanSeriesDirectoryFormat">BooleanSeriesDirectoryFormat</option> <option value="BooleanSeriesFormat">BooleanSeriesFormat</option> <option value="CasavaOneEightLanelessPerSampleDirFmt">CasavaOneEightLanelessPerSampleDirFmt</option> <option value="CasavaOneEightSingleLanePerSampleDirFmt">CasavaOneEightSingleLanePerSampleDirFmt</option> <option value="DADA2StatsDirFmt">DADA2StatsDirFmt</option> <option value="DADA2StatsFormat">DADA2StatsFormat</option> <option value="DNAFASTAFormat">DNAFASTAFormat</option> <option value="DNASequencesDirectoryFormat">DNASequencesDirectoryFormat</option> <option value="DeblurStatsDirFmt">DeblurStatsDirFmt</option> <option value="DeblurStatsFmt">DeblurStatsFmt</option> <option value="DistanceMatrixDirectoryFormat">DistanceMatrixDirectoryFormat</option> <option value="EMPPairedEndCasavaDirFmt">EMPPairedEndCasavaDirFmt</option> <option value="EMPPairedEndDirFmt">EMPPairedEndDirFmt</option> <option value="EMPSingleEndCasavaDirFmt">EMPSingleEndCasavaDirFmt</option> <option value="EMPSingleEndDirFmt">EMPSingleEndDirFmt</option> <option value="ErrorCorrectionDetailsDirFmt">ErrorCorrectionDetailsDirFmt</option> <option value="FastqGzFormat">FastqGzFormat</option> <option value="FirstDifferencesDirectoryFormat">FirstDifferencesDirectoryFormat</option> <option value="FirstDifferencesFormat">FirstDifferencesFormat</option> <option value="HeaderlessTSVTaxonomyDirectoryFormat">HeaderlessTSVTaxonomyDirectoryFormat</option> <option value="HeaderlessTSVTaxonomyFormat">HeaderlessTSVTaxonomyFormat</option> <option value="ImportanceDirectoryFormat">ImportanceDirectoryFormat</option> <option value="ImportanceFormat">ImportanceFormat</option> <option value="LSMatFormat">LSMatFormat</option> <option value="MultiplexedPairedEndBarcodeInSequenceDirFmt">MultiplexedPairedEndBarcodeInSequenceDirFmt</option> <option value="MultiplexedSingleEndBarcodeInSequenceDirFmt">MultiplexedSingleEndBarcodeInSequenceDirFmt</option> <option value="NewickDirectoryFormat">NewickDirectoryFormat</option> <option value="NewickFormat">NewickFormat</option> <option value="OrdinationDirectoryFormat">OrdinationDirectoryFormat</option> <option value="OrdinationFormat">OrdinationFormat</option> <option value="PairedDNASequencesDirectoryFormat">PairedDNASequencesDirectoryFormat</option> <option value="PairedEndFastqManifestPhred33">PairedEndFastqManifestPhred33</option> <option value="PairedEndFastqManifestPhred33V2">PairedEndFastqManifestPhred33V2</option> <option value="PairedEndFastqManifestPhred64">PairedEndFastqManifestPhred64</option> <option value="PairedEndFastqManifestPhred64V2">PairedEndFastqManifestPhred64V2</option> <option value="PlacementsDirFmt">PlacementsDirFmt</option> <option value="PlacementsFormat">PlacementsFormat</option> <option value="PredictionsDirectoryFormat">PredictionsDirectoryFormat</option> <option value="PredictionsFormat">PredictionsFormat</option> <option value="QIIME1DemuxDirFmt">QIIME1DemuxDirFmt</option> <option value="QIIME1DemuxFormat">QIIME1DemuxFormat</option> <option value="QualityFilterStatsDirFmt">QualityFilterStatsDirFmt</option> <option value="QualityFilterStatsFmt">QualityFilterStatsFmt</option> <option value="SampleEstimatorDirFmt">SampleEstimatorDirFmt</option> <option value="SingleEndFastqManifestPhred33">SingleEndFastqManifestPhred33</option> <option value="SingleEndFastqManifestPhred33V2">SingleEndFastqManifestPhred33V2</option> <option value="SingleEndFastqManifestPhred64">SingleEndFastqManifestPhred64</option> <option value="SingleEndFastqManifestPhred64V2">SingleEndFastqManifestPhred64V2</option> <option value="SingleLanePerSamplePairedEndFastqDirFmt">SingleLanePerSamplePairedEndFastqDirFmt</option> <option value="SingleLanePerSampleSingleEndFastqDirFmt">SingleLanePerSampleSingleEndFastqDirFmt</option> <option value="TSVTaxonomyDirectoryFormat">TSVTaxonomyDirectoryFormat</option> <option value="TSVTaxonomyFormat">TSVTaxonomyFormat</option> <option value="TaxonomicClassiferTemporaryPickleDirFmt">TaxonomicClassiferTemporaryPickleDirFmt</option> <option value="UchimeStatsDirFmt">UchimeStatsDirFmt</option> <option value="UchimeStatsFmt">UchimeStatsFmt</option> </param> </inputs> <outputs> <data format="qza" label="${tool.name} on ${on_string}: outputpath.qza" name="outputpath"/> </outputs> <!--<stdio> <exit_code range="2" level="warning" description="Error" /> </stdio>--> <help> </help> <macros> <import>qiime_citation.xml</import> </macros> <expand macro="qiime_citation" /> </tool>