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1 <?xml version="1.0" ?>
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2 <tool id="qiime_feature-classifier_classify-consensus-vsearch" name="qiime feature-classifier classify-consensus-vsearch" version="2018.4">
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3 <description> - VSEARCH consensus taxonomy classifier</description>
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4 <requirements>
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5 <requirement type="package" version="2018.4">qiime2</requirement>
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6 </requirements>
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7 <command><![CDATA[
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8 qiime feature-classifier classify-consensus-vsearch --i-query=$iquery
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9
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10 #if str( $id_to_taxonomy_fp.selector ) == 'history'
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11 #set $tax = $id_to_taxonomy_fp.taxonomy_fp
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12 --i-reference-taxonomy '$tax'
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13 #else:
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14 #set $tax = $id_to_taxonomy_fp.taxonomy_fp.fields.path
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15 --i-reference-taxonomy '$tax'
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16 #end if
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17
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18 --i-reference-reads=$ireferencereads
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19 #if $ppercidentity:
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20 --p-perc-identity=$ppercidentity
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21 #end if
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22
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23 #if str($cmdconfig) != 'None':
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24 --cmd-config=$cmdconfig
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25 #end if
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26
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27 #if $pminconsensus:
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28 --p-min-consensus=$pminconsensus
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29 #end if
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30
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31 #if str($pstrand) != 'None':
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32 --p-strand=$pstrand
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33 #end if
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34
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35 --o-classification=oclassification
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36
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37 #if str($punassignablelabel):
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38 --p-unassignable-label="$punassignablelabel"
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39 #end if
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40
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41 #set $pthreads = '${GALAXY_SLOTS:-4}'
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42 --p-threads="$pthreads"
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43
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44 #if $pmaxaccepts:
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45 --p-maxaccepts=$pmaxaccepts
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46 #end if
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47 ;
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48 cp oclassification.qza $oclassification;
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49 ]]></command>
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50 <inputs>
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51 <param format="qza,no_unzip.zip" label="--i-query: FeatureData[Sequence] Sequences to classify taxonomically. [required]" name="iquery" optional="False" type="data"/>
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52 <param format="qza,no_unzip.zip" label="--i-reference-reads: FeatureData[Sequence] reference sequences. [required]" name="ireferencereads" optional="False" type="data"/>
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53
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54 <conditional name="id_to_taxonomy_fp" optional="True">
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55 <param name="selector" type="select" label="Reference taxonomy to query">
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56 <option value="cached">Public databases</option>
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57 <option value="history">Databases from your history</option>
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58 </param>
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59 <when value="cached">
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60 <param argument="--taxonomy_fp" label="Reference taxonomy" type="select" optional="True">
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61 <options from_data_table="qiime_taxonomy" />
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62 </param>
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63 </when>
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64 <when value="history">
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65 <param argument="--taxonomy_fp" type="data" format="qza,no_unzip.zip" label="Reference databases" optional="True" />
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66 </when>
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67 </conditional>
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68
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69 <param label="--p-maxaccepts: Maximum number of hits to keep for each query. Must be in range [0, infinity]. [default: 10]" name="pmaxaccepts" optional="True" type="integer" value="10"/>
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70 <param label="--p-perc-identity: Reject match if percent identity to query is lower. Must be in range [0.0, 1.0]. [default: 0.8]" name="ppercidentity" optional="True" type="float" value="0.8"/>
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71 <param label="--p-strand: [both|plus] Align against reference sequences in forward ('plus') or both directions ('both'). [default: both]" name="pstrand" optional="True" type="select">
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72 <option selected="True" value="both">both</option>
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73 <option value="plus">plus</option>
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74 </param>
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75 <param label="--p-min-consensus: Minimum fraction of assignments must match top hit to be accepted as consensus assignment. Must be in range (0.5, 1.0]. [default: 0.51]" name="pminconsensus" optional="True" type="float" value="0.51"/>
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76 <param label="--p-unassignable-label: [default: Unassigned]" name="punassignablelabel" optional="True" type="text" value="Unassigned"/>
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77 <param label="--cmd-config: Use config file for command options" name="cmdconfig" optional="True" type="data"/>
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78 </inputs>
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79 <outputs>
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80 <data format="qza" label="${tool.name} on ${on_string}: classification.qza" name="oclassification"/>
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81 </outputs>
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82 <help><![CDATA[
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83 VSEARCH consensus taxonomy classifier
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84 -------------------------------------
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85
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86 Assign taxonomy to query sequences using VSEARCH. Performs VSEARCH global
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87 alignment between query and reference_reads, then assigns consensus
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88 taxonomy to each query sequence from among maxaccepts top hits,
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89 min_consensus of which share that taxonomic assignment.
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90
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91 Parameters
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92 ----------
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93 query : FeatureData[Sequence]
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94 Sequences to classify taxonomically.
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95 reference_reads : FeatureData[Sequence]
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96 reference sequences.
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97 reference_taxonomy : FeatureData[Taxonomy]
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98 reference taxonomy labels.
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99 maxaccepts : Int % Range(1, None), optional
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100 Maximum number of hits to keep for each query. Must be in range [0,
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101 infinity].
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102 perc_identity : Float % Range(0.0, 1.0, inclusive_end=True), optional
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103 Reject match if percent identity to query is lower. Must be in range
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104 [0.0, 1.0].
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105 strand : Str % Choices({'both', 'plus'}), optional
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106 Align against reference sequences in forward ("plus") or both
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107 directions ("both").
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108 min_consensus : Float % Range(0.5, 1.0, inclusive_start=False, inclusive_end=True), optional
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109 Minimum fraction of assignments must match top hit to be accepted as
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110 consensus assignment. Must be in range (0.5, 1.0].
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111 unassignable_label : Str, optional
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112 \
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113
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114 Returns
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115 -------
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116 classification : FeatureData[Taxonomy]
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117 The resulting taxonomy classifications.
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118 ]]>
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119 </help>
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120 <macros>
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121 <import>qiime_citation.xml</import>
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122 </macros>
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123 <expand macro="qiime_citation" />
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124 </tool>
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