Mercurial > repos > florianbegusch > qiime2_wrappers
diff qiime2/qiime_vsearch_join-pairs.xml @ 2:149432539226 draft
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author | florianbegusch |
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date | Wed, 17 Jul 2019 01:49:31 -0400 |
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children | 71f124e02000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_vsearch_join-pairs.xml Wed Jul 17 01:49:31 2019 -0400 @@ -0,0 +1,139 @@ +<?xml version="1.0" ?> +<tool id="qiime_vsearch_join-pairs" name="qiime vsearch join-pairs" version="2019.4"> + <description> - Join paired-end reads.</description> + <requirements> + <requirement type="package" version="2019.4">qiime2</requirement> + </requirements> + <command><![CDATA[ +qiime vsearch join-pairs + +--i-demultiplexed-seqs=$idemultiplexedseqs + +#if str($ptruncqual): + --p-truncqual="$ptruncqual" +#end if + +#if $pminlen: + --p-minlen=$pminlen +#end if + +#if str($pmaxns): + --p-maxns="$pmaxns" +#end if + +#if $pallowmergestagger: + --p-allowmergestagger +#end if + +#if $pminovlen: + --p-minovlen=$pminovlen +#end if + +#if $pmaxdiffs: + --p-maxdiffs=$pmaxdiffs +#end if + +#if str($pminmergelen): + --p-minmergelen="$pminmergelen" +#end if + +#if str($pmaxmergelen): + --p-maxmergelen="$pmaxmergelen" +#end if + +#if str($pmaxee): + --p-maxee="$pmaxee" +#end if + +#if $pqmin: + --p-qmin=$pqmin +#end if + +#if $pqminout: + --p-qminout=$pqminout +#end if + +#if $pqmax: + --p-qmax=$pqmax +#end if + +#if $pqmaxout: + --p-qmaxout=$pqmaxout +#end if + +--o-joined-sequences=ojoinedsequences +; +cp ojoinedsequences.qza $ojoinedsequences + ]]></command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-demultiplexed-seqs: ARTIFACT SampleData[PairedEndSequencesWithQuality] The demultiplexed paired-end sequences to be joined. [required]" name="idemultiplexedseqs" optional="False" type="data"/> + <param label="--p-truncqual: INTEGER Truncate sequences at the first base with the Range(0, None) specified quality score value or lower. [optional]" name="ptruncqual" optional="True" min="0" type="integer"/> + <param label="--p-minlen: INTEGER Sequences shorter than minlen after truncation are Range(0, None) discarded. [default: 1]" name="pminlen" optional="True" type="integer" min="0" value="1"/> + <param label="--p-maxns: INTEGER Sequences with more than maxns N characters are Range(0, None) discarded. [optional]" name="pmaxns" optional="True" min="0" type="integer"/> + <param label="--p-allowmergestagger: --p-no-allowmergestagger Allow joining of staggered read pairs. [default: False]" name="pallowmergestagger" selected="False" type="boolean"/> + <param label="--p-minovlen: INTEGER Minimum overlap length of forward and reverse reads Range(0, None) for joining. [default: 10]" name="pminovlen" optional="True" type="integer" min="0" value="10"/> + <param label="--p-maxdiffs: INTEGER Maximum number of mismatches in the forward/reverse Range(0, None) read overlap for joining. [default: 10]" name="pmaxdiffs" optional="True" type="integer" min="0" value="10"/> + <param label="--p-minmergelen: INTEGER Range(0, None) Minimum length of the joined read to be retained. [optional]" name="pminmergelen" optional="True" min="0" type="integer"/> + <param label="--p-maxmergelen: INTEGER Range(0, None) Maximum length of the joined read to be retained. [optional]" name="pmaxmergelen" optional="True" min="0" type="integer"/> + <param label="--p-maxee: NUMBER Maximum number of expected errors in the joined read Range(0.0, None) to be retained. [optional]" name="pmaxee" optional="True" min="0" type="float"/> + <param label="--p-qmin: INTEGER Range(-5, 2, inclusive_end=True) The minimum allowed quality score in the input. [default: 0]" name="pqmin" optional="True" type="integer" min="-5" max="2" exclude_max="False" value="0"/> + <param label="--p-qminout: INTEGER Range(-5, 2, inclusive_end=True) The minimum allowed quality score to use in output. [default: 0]" name="pqminout" optional="True" type="integer" min="-5" max="2" exclude_max="False" value="0"/> + <param label="--p-qmax: INTEGER Range(40, 41, inclusive_end=True) The maximum allowed quality score in the input. [default: 41]" name="pqmax" optional="True" type="integer" min="40" max="41" exclude_max="False" value="41"/> + <param label="--p-qmaxout: INTEGER Range(40, 41, inclusive_end=True) The maximum allowed quality score to use in output. [default: 41]" name="pqmaxout" optional="True" type="integer" min="40" max="41" exclude_max="False" value="41"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: joinedsequences.qza" name="ojoinedsequences"/> + </outputs> + <help><![CDATA[ +Join paired-end reads. +###################### + +Join paired-end sequence reads using vsearch's merge_pairs function. The +qmin, qminout, qmax, and qmaxout parameters should only need to be modified +when working with older fastq sequence data. See the vsearch documentation +for details on how paired-end joining is performed, and for more +information on the parameters to this method. + +Parameters +---------- +demultiplexed_seqs : SampleData[PairedEndSequencesWithQuality] + The demultiplexed paired-end sequences to be joined. +truncqual : Int % Range(0, None), optional + Truncate sequences at the first base with the specified quality score + value or lower. +minlen : Int % Range(0, None), optional + Sequences shorter than minlen after truncation are discarded. +maxns : Int % Range(0, None), optional + Sequences with more than maxns N characters are discarded. +allowmergestagger : Bool, optional + Allow joining of staggered read pairs. +minovlen : Int % Range(0, None), optional + Minimum overlap length of forward and reverse reads for joining. +maxdiffs : Int % Range(0, None), optional + Maximum number of mismatches in the forward/reverse read overlap for + joining. +minmergelen : Int % Range(0, None), optional + Minimum length of the joined read to be retained. +maxmergelen : Int % Range(0, None), optional + Maximum length of the joined read to be retained. +maxee : Float % Range(0.0, None), optional + Maximum number of expected errors in the joined read to be retained. +qmin : Int % Range(-5, 2, inclusive_end=True), optional + The minimum allowed quality score in the input. +qminout : Int % Range(-5, 2, inclusive_end=True), optional + The minimum allowed quality score to use in output. +qmax : Int % Range(40, 41, inclusive_end=True), optional + The maximum allowed quality score in the input. +qmaxout : Int % Range(40, 41, inclusive_end=True), optional + The maximum allowed quality score to use in output. + +Returns +------- +joined_sequences : SampleData[JoinedSequencesWithQuality] + The joined sequences. + ]]></help> +<macros> + <import>qiime_citation.xml</import> +</macros> +<expand macro="qiime_citation"/> +</tool>