Mercurial > repos > florianbegusch > qiime2_wrappers
diff qiime2/qiime_vsearch_uchime-denovo.xml @ 0:51b9b6b57732 draft
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author | florianbegusch |
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date | Thu, 24 May 2018 05:21:07 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2/qiime_vsearch_uchime-denovo.xml Thu May 24 05:21:07 2018 -0400 @@ -0,0 +1,103 @@ +<?xml version="1.0" ?> +<tool id="qiime_vsearch_uchime-denovo" name="qiime vsearch uchime-denovo" version="2018.4"> + <description> - De novo chimera filtering with vsearch.</description> + <requirements> + <requirement type="package" version="2018.4">qiime2</requirement> + </requirements> + <command> + <![CDATA[ + qiime vsearch uchime-denovo --i-table=$itable --i-sequences=$isequences + + #if $pdn: + --p-dn=$pdn + #end if + --o-nonchimeras=ononchimeras --o-stats=ostats --o-chimeras=ochimeras + #if $pmindiffs: + --p-mindiffs=$pmindiffs + #end if + + #if $pminh: + --p-minh=$pminh + #end if + + #if str($cmdconfig) != 'None': + --cmd-config=$cmdconfig + #end if + + #if $pmindiv: + --p-mindiv=$pmindiv + #end if + + #if $pxn: + --p-xn=$pxn + #end if + ; + cp ononchimeras.qza $ononchimeras; + cp ostats.qza $ostats; + cp ochimeras.qza $ochimeras; + ]]> + </command> + <inputs> + <param format="qza,no_unzip.zip" label="--i-sequences: FeatureData[Sequence] The feature sequences to be chimera-checked. [required]" name="isequences" optional="False" type="data"/> + <param format="qza,no_unzip.zip" label="--i-table: FeatureTable[Frequency] Feature table (used for computing total feature abundances). [required]" name="itable" optional="False" type="data"/> + + <param label="--p-dn: No vote pseudo-count, corresponding to the parameter n in the chimera scoring function. [default: 1.4]" name="pdn" optional="True" type="float" value="1.4"/> + + <param label="--p-mindiffs: Minimum number of differences per segment. [default: 3]" name="pmindiffs" optional="True" type="integer" value="3"/> + <param label="--p-mindiv: Minimum divergence from closest parent. [default: 0.8]" name="pmindiv" optional="True" type="float" value="0.8"/> + <param label="--p-minh: Minimum score (h). Increasing this value tends to reduce the number of false positives and to decrease sensitivity. [default: 0.28]" name="pminh" optional="True" type="float" value="0.28"/> + + <param label="--p-xn: No vote weight, corresponding to the parameter beta in the scoring function. [default: 8.0]" name="pxn" optional="True" type="float" value="8.0"/> + + <param label="--cmd-config: Use config file for command options" name="cmdconfig" optional="True" type="data"/> + </inputs> + <outputs> + <data format="qza" label="${tool.name} on ${on_string}: non-chimeras.qza" name="ononchimeras"/> + <data format="qza" label="${tool.name} on ${on_string}: stats.qza" name="ostats"/> + <data format="qza" label="${tool.name} on ${on_string}: chimeras.qza" name="ochimeras"/> + </outputs> + <help> + <![CDATA[ +De novo chimera filtering with vsearch. +---------------------------------------- + +Apply the vsearch uchime_denovo method to identify chimeric feature +sequences. The results of this method can be used to filter chimeric +features from the corresponding feature table. For additional details, +please refer to the vsearch documentation. + +Parameters +---------- +sequences : FeatureData[Sequence] + The feature sequences to be chimera-checked. +table : FeatureTable[Frequency] + Feature table (used for computing total feature abundances). +dn : Float % Range(0.0, None), optional + No vote pseudo-count, corresponding to the parameter n in the chimera + scoring function. +mindiffs : Int % Range(1, None), optional + Minimum number of differences per segment. +mindiv : Float % Range(0.0, None), optional + Minimum divergence from closest parent. +minh : Float % Range(0.0, 1.0, inclusive_end=True), optional + Minimum score (h). Increasing this value tends to reduce the number of + false positives and to decrease sensitivity. +xn : Float % Range(1.0, None, inclusive_start=False), optional + No vote weight, corresponding to the parameter beta in the scoring + function. + +Returns +------- +chimeras : FeatureData[Sequence] + The chimeric sequences. +nonchimeras : FeatureData[Sequence] + The non-chimeric sequences. +stats : UchimeStats + Summary statistics from chimera checking. + ]]> + </help> +<macros> + <import>qiime_citation.xml</import> +</macros> +<expand macro="qiime_citation" /> +</tool>