<?xml version="1.0" ?>
<tool id="qiime_demux_emp-single" name="qiime demux emp-single" version="2018.4">
	<description>- Demultiplex sequence data generated with the EMP protocol.</description>
	<requirements>
		<requirement type="package" version="2018.4">qiime2</requirement>
	</requirements>
	<command><![CDATA[
  qiime demux emp-single --m-barcodes-column="$mbarcodescolumn" --i-seqs=$iseqs
  
  #def list_dict_to_string(list_dict):
  	#set $file_list = list_dict[0]['additional_input'].__getattr__('file_name')
  	#for d in list_dict[1:]:
  		#set $file_list = $file_list + ',' + d['additional_input'].__getattr__('file_name')
  	#end for
  	#return $file_list
  #end def
  
   --m-barcodes-file=$list_dict_to_string($input_files_mbarcodesfile) --o-per-sample-sequences=opersamplesequences
   
  #if $prevcompbarcodes:
   --p-rev-comp-barcodes
  #else
    --p-no-rev-comp-barcodes
  #end if
  
  #if $prevcompmappingbarcodes:
   --p-rev-comp-mapping-barcodes
  #else
    --p-no-rev-comp-mapping-barcodes
  #end if
  
  #if str($cmdconfig) != 'None':
   --cmd-config=$cmdconfig
  #end if
  ;
  cp opersamplesequences.qza $opersamplesequences;
  ]]></command>
	<inputs>
		<param format="qza,no_unzip.zip" label="--i-seqs: EMPPairedEndSequences | EMPSingleEndSequences | RawSequences The single-end sequences to be demultiplexed.  [required]" name="iseqs" optional="False" type="data"/>
		<repeat name="input_files_mbarcodesfile" optional="False" title="--m-barcodes-file">
			<param label="--m-barcodes-file: Metadata file or artifact viewable as metadata. This option may be supplied multiple times to merge metadata. [required]" name="additional_input" type="data" format="tabular,qza,no_unzip.zip"/>
		</repeat>
		<param label="--m-barcodes-column: MetadataColumn[Categorical] Column from metadata file or artifact viewable as metadata. The sample metadata column containing the per-sample barcodes. [required]" name="mbarcodescolumn" optional="False" type="text"/>
		<param label="--p-rev-comp-barcodes: --p-no-rev-comp-barcodes If provided, the barcode sequence reads will be reverse complemented prior to demultiplexing.  [default: False]" name="prevcompbarcodes" checked="False" type="boolean"/>
		<param label="--p-rev-comp-mapping-barcodes: --p-no-rev-comp-mapping-barcodes If provided, the barcode sequences in the sample metadata will be reverse complemented prior to demultiplexing.  [default: False]" name="prevcompmappingbarcodes" checked="False" type="boolean"/>
		<param label="--cmd-config: Use config file for command options" name="cmdconfig" optional="True" type="data"/>
	</inputs>
	<outputs>
		<data format="qza" label="${tool.name} on ${on_string}: per-sample-sequences.qza" name="opersamplesequences"/>
	</outputs>
	<help><![CDATA[
Demultiplex sequence data generated with the EMP protocol.
----------------------------------------------------------

Demultiplex sequence data (i.e., map barcode reads to sample ids) for data
generated with the Earth Microbiome Project (EMP) amplicon sequencing
protocol. Details about this protocol can be found at
http://www.earthmicrobiome.org/protocols-and-standards/

Parameters
----------
seqs : EMPPairedEndSequences | EMPSingleEndSequences | RawSequences
    The single-end sequences to be demultiplexed.
barcodes : MetadataColumn[Categorical]
    The sample metadata column containing the per-sample barcodes.
rev_comp_barcodes : Bool, optional
    If provided, the barcode sequence reads will be reverse complemented
    prior to demultiplexing.
rev_comp_mapping_barcodes : Bool, optional
    If provided, the barcode sequences in the sample metadata will be
    reverse complemented prior to demultiplexing.

Returns
-------
per_sample_sequences : SampleData[SequencesWithQuality]
    The resulting demultiplexed sequences.
      ]]>
	</help>
<macros>
	<import>qiime_citation.xml</import>
</macros>
<expand macro="qiime_citation" />
</tool>