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comparison blasttoolssearch/blasttoolssearch.xml @ 4:2051ee2bedc4 draft

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author fubar
date Wed, 19 Jul 2023 06:45:26 +0000
parents a6593725b728
children 3a499f3ed69c
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3:a6593725b728 4:2051ee2bedc4
96 96
97 Wraps https://github.com/schmidda/blast-tools/tree/master as a Galaxy tool as a demonstration for Roberto Barrero 97 Wraps https://github.com/schmidda/blast-tools/tree/master as a Galaxy tool as a demonstration for Roberto Barrero
98 98
99 99
100 100
101 ------
102
103
104 Script::
105
106 ## eResearch Office, QUT
107 ## Created: 31 March 2021
108 ## Last modified: 28 September 2022
109 ## Script: Processes Galaxy Australia generated blastN outputs to summarise and report hits to REGULATED and ENDEMIC viruses/viroids.
110 ## Usage: ./run_VirReport_Summary.sh
111 ## changed to accept a single input file name passed as $1
112 ## Ross Lazarus for a ToolFactory wrapper for Robert Barrero
113 ## July 18 2023
114 dataPath=${PWD}
115 # Requirement: One or more GA-VSD .tabular outputs need to be in the folder where the command above (Usage)is executed.
116 # The script will Look for all files with the suffix *.tabular
117 #Processing tabular files
118 file=$1
119 var=$(basename $file)
120 #STEP0: fetch Top 1 Hits
121 cat $file | awk '{print $1}' | sort | uniq > ${var}.top1.ids
122 for i in `cat ${var}.top1.ids`
123 do
124 echo "fetching top hits..." $i;
125 grep $i $file | head -1 >> ${var}.top1Hits.txt;
126 done
127 #STEP1: modify the columns of Galaxy Australia (GA) blast output to the expected format by the BlastTools.jar tool
128 ###### namely: qseqid sgi sacc length pident mismatch gapopen qstart qend qlen sstart send slen sstrand evalue bitscore qcovhsp stitle staxids qseq sseq sseqid qcovs qframe sframe
129 cat ${var}.top1Hits.txt |csvtk cut -H -t -f 1,19,20,4,3,5,6,7,8,17,9,10,18,22,11,12,24,21,25,15,16,2,23,13,14 | sed 's/ /_/g' > ${var}.txt
130 #STEP2: summarise the GA blastN files
131 #java -jar /mnt/c/Users/lelwala/HTS/BlastTools.jar -t blastn ${var}.txt
132 java -jar $3 -t blastn ${var}.txt
133 #filter virus/viroid/endo
134 cat summary_${var}.txt | grep "virus\|viroid\|endo" > summary_${var}_filtered.txt
135 #STEP3: fetch unique names from Blast summary reports
136 cat summary_${var}_filtered.txt | awk '{print $7}' | awk -F "|" '{print $2}'| sort | uniq | sed 's/Species://' > ${var}_uniq.ids
137 #STEP4: retrieve the best hit for each virus/viroid
138 echo "processing top hits ..."
139 touch ${var}_filtered.txt
140 for id in `cat ${var}_uniq.ids`
141 do
142 #print on the screen the name of the virus/viroids to search
143 #echo "fetching species matches ..." $id
144 #fetch the virus name on the summary_blastn file by selecting the longest alignment (column 3) and highest genome coverage (column 5)
145 grep $id summary_${var}.txt | sort -k3,3nr -k5,5nr | head -1 >> ${var}_filtered.txt
146 done
147 #print the header of the inital summary_blastn file
148 cat summary_${var}.txt | head -1 > header
149 #report 1
150 cat header ${var}_filtered.txt > $2
151 #removing intermediate files
152 rm summary_${file}.txt ${file}.txt ${file}.top1.ids ${file}_uniq.ids summary_${file}_filtered.txt header* ${var}_filtered.txt *top1Hits.txt
153
154 ]]></help> 101 ]]></help>
155 <citations> 102 <citations>
156 <citation type="doi">10.1093/bioinformatics/bts573</citation> 103 <citation type="doi">10.1093/bioinformatics/bts573</citation>
157 </citations> 104 </citations>
158 </tool> 105 </tool>