Mercurial > repos > fubar > clustalw
diff rgclustal/rgClustalw.xml @ 3:9c4d943fbef7 draft default tip
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author | fubar |
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date | Sun, 01 Dec 2013 20:07:58 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/rgclustal/rgClustalw.xml Sun Dec 01 20:07:58 2013 -0500 @@ -0,0 +1,128 @@ +<tool id="clustalw" name="ClustalW" version="0.2"> + <description>multiple sequence alignment program for DNA or proteins</description> + <requirements> + <requirement type="package" version="2.1">clustalw</requirement> + </requirements> + <command> + #if ($range.mode=="part") + clustalw2 -infile=$input -outfile=$output -OUTORDER=$out_order -RANGE=$range.seq_range_start,$range.seq_range_end + #elif ($range.mode=="complete") + clustalw2 -infile=$input -outfile=$output -OUTORDER=$out_order + #end if + #if ($outcontrol.outform=="clustal") + -SEQNOS=$outcontrol.out_seqnos + #end if + #if ($outcontrol.outform=="phylip") + -OUTPUT=PHYLIP + #end if + #if ($outcontrol.outform=="fasta") + -OUTPUT=FASTA + #end if + -TYPE=$dnarna 1>$outlog 2>&1 + </command> + <inputs> + <page> + <param format="fasta" name="input" type="data" label="Fasta File" /> + <param name="outname" label="Name for output files to make it easy to remember what you did" type="text" size="50" value="Clustal_run" /> + <param name="dnarna" type="select" label="Data Type"> + <option value="DNA" selected="True">DNA nucleotide sequences</option> + <option value="PROTEIN">Protein sequences</option> + </param> + <conditional name="outcontrol"> + <param name="outform" type="select" label="Output alignment format"> + <option value="clustal" selected="True">Native Clustal output format</option> + <option value="phylip">Phylip format</option> + <option value="fasta">Fasta format</option> + </param> + <when value="fasta" /> + <when value="phylip" /> + <when value="clustal"> + <param name="out_seqnos" type="select" label="Show residue numbers in clustal format output"> + <option value="ON">yes</option> + <option value="OFF" selected="true">no</option> + </param> + </when> + </conditional> + <param name="out_order" type="select" label="Output Order"> + <option value="ALIGNED">aligned</option> + <option value="INPUT">same order as input file</option> + </param> + + <conditional name="range"> + <param name="mode" type="select" label="Output complete alignment (or specify part to output)"> + <option value="complete">complete alignment</option> + <option value="part">only part of the alignment</option> + </param> + <when value="complete"> + </when> + <when value="part"> + <param name="seq_range_start" size="5" type="integer" value="1" label="start point" help="sequence range to write"> + </param> + <param name="seq_range_end" size="5" type="integer" value="99999" label="end point" > + </param> + </when> + </conditional> + </page> + </inputs> + <outputs> + <data format="clustal" name="output" label="${outname}_output.${outcontrol.outform}"> + <change_format> + <when input="outcontrol.outform" value="phylip" format="phylip" /> + <when input="outcontrol.outform" value="fasta" format="fasta" /> + </change_format> + </data> + <data format="txt" name="outlog" label="${outname}_clustal_log.txt"/> + </outputs> + <tests> + <test> + <param name="input" value="rgClustal_testin.fasta" /> + <param name = "outname" value="" /> + <param name = "outform" value="fasta" /> + <param name = "dnarna" value="DNA" /> + <param name = "mode" value="complete" /> + <param name = "out_order" value="ALIGNED" /> + <output name="output" file="rgClustal_testout.fasta" ftype="fasta" /> + <output name="output" file="rgClustal_testout.log" ftype="txt" lines_diff="5" /> + </test> + </tests> + <help> + +**Note** + +This tool allows you to run a multiple sequence alignment with ClustalW2 (see Clustsrc_) using the default options. + +For a tutorial introduction, see ClustalW2_ + +You can align DNA or protein sequences in the input file which should be multiple sequences to be aligned in a fasta file + +A log will be output to your history showing the output Clustal would normally write to standard output. + +The alignments will appear as a clustal format file or optionally, as phylip or fasta format files in your history. If you choose fasta as +the output format, you can create a 'Logo' image using the Sequence Logo tool. + +If Clustal format is chosen, you have the option of adding basepair counts to the output + +A subsequence of the alignment can be output by setting the Output complete parameter to "Partial" and defining the offset and end of the subsequence to be output + +---- + +**Attribution** + +Clustal attribution and associated documentation are available at Clustsrc_ + +The first iteration of this Galaxy wrapper was written by Hans-Rudolf Hotz - see Clustfirst_ + +It was modified by Ross Lazarus for the rgenetics project + +This wrapper is now LGPL + +.. _ClustalW2: http://www.ebi.ac.uk/2can/tutorials/protein/clustalw.html + +.. _Clustsrc: http://www.clustal.org + +.. _Clustfirst: http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-November/003732.html + + </help> + +</tool> +