Mercurial > repos > fubar > clustalw
diff clustalw-76de6971a0de/rgclustal/README @ 1:cf59a9ae0efe draft
From test toolshed - appears to install fine.
author | fubar |
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date | Sun, 01 Dec 2013 19:57:03 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/clustalw-76de6971a0de/rgclustal/README Sun Dec 01 19:57:03 2013 -0500 @@ -0,0 +1,55 @@ +Updated july 11 2013 for automated toolshed installation + +** This is a wrapper for ClustalW ** + +This tool allows you to align multiple sequences in Galaxy, using ClustalW2_ with mostly default options which should work reasonably well for many alignments. +DNA or protein sequences can be aligned. The input file must be a fasta file in your current history. + +The alignments will appear as a clustal format file or optionally, as phylip or fasta format files in your history and a text log will be output to your history +showing the output Clustalw would normally write to standard output. + +If Clustal format is chosen, you have the option of adding basepair counts to the output + +A subsequence of the alignment can be output by setting the Output complete parameter to "Partial" and defining the offset and end of the subsequence to be output + +**Installation** + +As of July 2013, automated installation from the tool shed should have worked. + +Otherwise, the old skool way was: + +Make sure clustalw2 is available on the path for all your nodes + +Move the test data files to your galaxy root test-data +Move the xml file to a subdirectory of your tools folder (eg rgenetics/) and then add a line in your tool_conf.xml to point there. +Run +sh run_functional_tests.sh -id clustalw +to make sure the tests work + +then restart Galaxy and you should be good to go. + +**Attribution** + +Clustal attribution and associated documentation are available at http://www.clustal.org + +An implementation of a Galaxy Clustal wrapper was written by Hans-Rudolf Hotz in an email on the developer list - +http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-November/003732.html + +This version by Ross Lazarus for the rgenetics project, builds on Hans-Rudolf's code, adding some additional controls and a log file. It also +deals with stderr so Cluastalw2 writing there doesn't cause the job to error out. That's encoded in the tail of the command line. + +**License** + +Assuming Hans-Rudolf is ok with a new license for this derived work, this version of his wrapper is LGPL like other rgenetics artefacts + +Written by Ross Lazarus for the Rgenetics project + +Copyright Ross Lazarus at gmail com 2011 + +All rights reserved. + +Released under the LGPL - see http://www.gnu.org/copyleft/lesser.html + + + +