Mercurial > repos > fubar > egapx_runner

annotate nf/subworkflows/ncbi/orthology/main.nf @ 5:6effccc966d0 draft

Find changesets by keywords (author, files, the commit message), revision number or hash, or revset expression.
planemo upload for repository https://github.com/ncbi/egapx commit 9e59da535540cb4d5c1c412bb2b0969744dfb0b0
author fubar
date Sun, 04 Aug 2024 01:59:37 +0000
parents d9c5c5b87fec
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
0
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
1 #!/usr/bin/env nextflow
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
2 // main nextflow script for EGAPx execution
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
3 // route data to subworkflows
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
4
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
5 nextflow.enable.dsl=2
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
6
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
7
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
8 include { extract_products_from_models } from './extract_products_from_models/main'
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
9 include { find_orthologs; fetch_ortholog_references; } from './find_orthologs/main'
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
10 include { diamond_orthology } from './diamond_orthology/main'
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
11 include { setup_genome; setup_proteins } from './../setup/main'
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
12 include { get_swiss_prot_ids as get_prot_ref_ids } from '../shared/diamond/main'
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
13
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
14 params.intermediate = false
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
15
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
16
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
17 workflow orthology_plane {
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
18 take:
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
19 genome_asnb
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
20 gencoll_asn
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
21 models
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
22 annot_files
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
23 task_params // task parameters for every task
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
24 main:
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
25 // Protein alignments
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
26 fetch_ortholog_references()
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
27 def (scaffolds_ref, gencoll_ref_asn, unpacked_genome_ref, genome_ref_asn, genome_ref_asnb) = setup_genome(fetch_ortholog_references.out.p14_genomic_fna, [], task_params.get('setup', [:]))
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
28 def (unpacked_proteins_ref, proteins_ref_asn, proteins_ref_asnb) = setup_proteins(fetch_ortholog_references.out.p14_protein_faa, task_params.get('setup', [:]))
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
29 def prot_ref_ids = get_prot_ref_ids(proteins_ref_asnb)
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
30 //orthology plane
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
31 extract_products_from_models(annot_files, task_params.get('extract_products_from_models', [:]))
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
32 // reference side. 1) gencoll, annotation , genome and protein sequence --> asn cashe or LDS demon
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
33
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
34 diamond_orthology(extract_products_from_models.out.prot_ids, prot_ref_ids , models, proteins_ref_asnb , task_params.get('diamond_orthology', [:]))
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
35
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
36 // input side 1) gencoll asn from setup, genome_asn from setup, protein from gnomon_wnode.out, annotation it is from annotbuilder annot_files or accepts_asn
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
37 find_orthologs( gencoll_asn, gencoll_ref_asn, annot_files, fetch_ortholog_references.out.annot_file, diamond_orthology.out.alignments, [],
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
38 models, proteins_ref_asnb , genome_asnb, genome_ref_asnb , task_params.get('find_orthologs', [:]))
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
39
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
40 emit:
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
41 orthologs = find_orthologs.out.orthologs
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
42 }
d9c5c5b87fec planemo upload for repository https://github.com/ncbi/egapx commit 8173d01b08d9a91c9ec5f6cb50af346edc8020c4
fubar
parents:
diff changeset
43