view x/static/js/4770.3d757d6b.chunk.js.map @ 125:49f3d3878413 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit 5ea1f9c1eef1de76232e69aa6d34cda77d90d566
author fubar
date Sat, 05 Oct 2024 23:58:05 +0000
parents
children
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/plugins/alignments/src/BamAdapter/BamSlightlyLazyFeature.ts","../../../plugins/alignments/src/BamAdapter/BamAdapter.ts"],"sourcesContent":["import {\n  Feature,\n  SimpleFeatureSerialized,\n} from '@jbrowse/core/util/simpleFeature'\nimport { BamRecord } from '@gmod/bam'\n\n// locals\nimport { getClip, getMismatches } from '../MismatchParser'\nimport BamAdapter from './BamAdapter'\n\nexport default class BamSlightlyLazyFeature implements Feature {\n  // uses parameter properties to automatically create fields on the class\n  // https://www.typescriptlang.org/docs/handbook/classes.html#parameter-properties\n  constructor(\n    private record: BamRecord,\n    private adapter: BamAdapter,\n    private ref?: string,\n  ) {}\n\n  _get_name() {\n    return this.record.get('name')\n  }\n\n  _get_type(): string {\n    return 'match'\n  }\n\n  _get_score(): number {\n    return this.record.get('mq')\n  }\n\n  _get_flags(): string {\n    return this.record.flags\n  }\n\n  _get_strand(): number {\n    return this.record.isReverseComplemented() ? -1 : 1\n  }\n\n  _get_pair_orientation() {\n    return this.record.isPaired() ? this.record.getPairOrientation() : undefined\n  }\n\n  _get_next_ref() {\n    return this.record.isPaired()\n      ? this.adapter.refIdToName(this.record._next_refid())\n      : undefined\n  }\n\n  _get_next_pos() {\n    return this.record.isPaired() ? this.record._next_pos() : undefined\n  }\n\n  _get_next_segment_position() {\n    return this.record.isPaired()\n      ? `${this.adapter.refIdToName(this.record._next_refid())}:${\n          this.record._next_pos() + 1\n        }`\n      : undefined\n  }\n\n  _get_seq() {\n    return this.record.getReadBases()\n  }\n\n  qualRaw() {\n    return this.record.qualRaw()\n  }\n\n  set() {}\n\n  tags() {\n    const properties = Object.getOwnPropertyNames(\n      BamSlightlyLazyFeature.prototype,\n    )\n\n    return [\n      ...new Set(\n        properties\n          .filter(\n            prop =>\n              prop.startsWith('_get_') &&\n              prop !== '_get_mismatches' &&\n              prop !== '_get_tags',\n          )\n          .map(methodName => methodName.replace('_get_', ''))\n          .concat(this.record._tags()),\n      ),\n    ]\n  }\n\n  id() {\n    return `${this.adapter.id}-${this.record.id()}`\n  }\n\n  get(field: string): any {\n    const methodName = `_get_${field}`\n    // @ts-expect-error\n    if (this[methodName]) {\n      // @ts-expect-error\n      return this[methodName]()\n    }\n    return this.record.get(field)\n  }\n\n  _get_refName() {\n    return this.adapter.refIdToName(this.record.seq_id())\n  }\n\n  parent() {\n    return undefined\n  }\n\n  children() {\n    return undefined\n  }\n\n  pairedFeature() {\n    return false\n  }\n\n  toJSON(): SimpleFeatureSerialized {\n    return {\n      ...Object.fromEntries(\n        this.tags()\n          .map(t => [t, this.get(t)])\n          .filter(elt => elt[1] !== undefined),\n      ),\n      uniqueId: this.id(),\n    }\n  }\n\n  _get_mismatches() {\n    return getMismatches(\n      this.get('CIGAR'),\n      this.get('MD'),\n      this.get('seq'),\n      this.ref,\n      this.qualRaw(),\n    )\n  }\n\n  _get_clipPos() {\n    const cigar = this.get('CIGAR') || ''\n    return getClip(cigar, this.get('strand'))\n  }\n}\n","import { BamFile } from '@gmod/bam'\nimport {\n  BaseFeatureDataAdapter,\n  BaseOptions,\n} from '@jbrowse/core/data_adapters/BaseAdapter'\nimport { Region } from '@jbrowse/core/util/types'\nimport { bytesForRegions, updateStatus, Feature } from '@jbrowse/core/util'\nimport { openLocation } from '@jbrowse/core/util/io'\nimport { ObservableCreate } from '@jbrowse/core/util/rxjs'\nimport { toArray } from 'rxjs/operators'\nimport { firstValueFrom } from 'rxjs'\n\n// locals\nimport BamSlightlyLazyFeature from './BamSlightlyLazyFeature'\nimport { IFilter } from '../shared'\n\ninterface Header {\n  idToName: string[]\n  nameToId: Record<string, number>\n}\n\nexport default class BamAdapter extends BaseFeatureDataAdapter {\n  private samHeader?: Header\n\n  private setupP?: Promise<Header>\n  private configureP?: Promise<{\n    bam: BamFile\n    sequenceAdapter?: BaseFeatureDataAdapter\n  }>\n\n  // derived classes may not use the same configuration so a custom configure\n  // method allows derived classes to override this behavior\n  protected async configurePre() {\n    const bamLocation = this.getConf('bamLocation')\n    const location = this.getConf(['index', 'location'])\n    const indexType = this.getConf(['index', 'indexType'])\n    const pm = this.pluginManager\n    const csi = indexType === 'CSI'\n    const bam = new BamFile({\n      bamFilehandle: openLocation(bamLocation, pm),\n      csiFilehandle: csi ? openLocation(location, pm) : undefined,\n      baiFilehandle: !csi ? openLocation(location, pm) : undefined,\n      yieldThreadTime: Number.POSITIVE_INFINITY,\n    })\n\n    const adapterConfig = this.getConf('sequenceAdapter')\n    if (adapterConfig && this.getSubAdapter) {\n      const { dataAdapter } = await this.getSubAdapter(adapterConfig)\n      return {\n        bam,\n        sequenceAdapter: dataAdapter as BaseFeatureDataAdapter,\n      }\n    }\n    return { bam }\n  }\n\n  protected async configure() {\n    if (!this.configureP) {\n      this.configureP = this.configurePre().catch((e: unknown) => {\n        this.configureP = undefined\n        throw e\n      })\n    }\n    return this.configureP\n  }\n\n  async getHeader(opts?: BaseOptions) {\n    const { bam } = await this.configure()\n    return bam.getHeaderText(opts)\n  }\n\n  private async setupPre(opts?: BaseOptions) {\n    const { statusCallback = () => {} } = opts || {}\n    const { bam } = await this.configure()\n    this.samHeader = await updateStatus(\n      'Downloading index',\n      statusCallback,\n      async () => {\n        const samHeader = await bam.getHeader(opts)\n\n        // use the @SQ lines in the header to figure out the\n        // mapping between ref ref ID numbers and names\n        const idToName: string[] = []\n        const nameToId: Record<string, number> = {}\n        samHeader\n          ?.filter(l => l.tag === 'SQ')\n          .forEach((sqLine, refId) => {\n            const SN = sqLine.data.find(item => item.tag === 'SN')\n            if (SN) {\n              // this is the ref name\n              const refName = SN.value\n              nameToId[refName] = refId\n              idToName[refId] = refName\n            }\n          })\n\n        return { idToName, nameToId }\n      },\n    )\n    return this.samHeader\n  }\n\n  async setup(opts?: BaseOptions) {\n    if (!this.setupP) {\n      this.setupP = this.setupPre(opts).catch((e: unknown) => {\n        this.setupP = undefined\n        throw e\n      })\n    }\n    return this.setupP\n  }\n\n  async getRefNames(opts?: BaseOptions) {\n    const { idToName } = await this.setup(opts)\n    return idToName\n  }\n\n  private async seqFetch(refName: string, start: number, end: number) {\n    const { sequenceAdapter } = await this.configure()\n    const refSeqStore = sequenceAdapter\n    if (!refSeqStore) {\n      return undefined\n    }\n    if (!refName) {\n      return undefined\n    }\n\n    const features = refSeqStore.getFeatures({\n      refName,\n      start,\n      end,\n      assemblyName: '',\n    })\n\n    const seqChunks = await firstValueFrom(features.pipe(toArray()))\n\n    let sequence = ''\n    seqChunks\n      .sort((a, b) => a.get('start') - b.get('start'))\n      .forEach(chunk => {\n        const chunkStart = chunk.get('start')\n        const chunkEnd = chunk.get('end')\n        const trimStart = Math.max(start - chunkStart, 0)\n        const trimEnd = Math.min(end - chunkStart, chunkEnd - chunkStart)\n        const trimLength = trimEnd - trimStart\n        const chunkSeq = chunk.get('seq') || chunk.get('residues')\n        sequence += chunkSeq.slice(trimStart, trimStart + trimLength)\n      })\n\n    if (sequence.length !== end - start) {\n      throw new Error(\n        `sequence fetch failed: fetching ${refName}:${(\n          start - 1\n        ).toLocaleString()}-${end.toLocaleString()} returned ${sequence.length.toLocaleString()} bases, but should have returned ${(\n          end - start\n        ).toLocaleString()}`,\n      )\n    }\n    return sequence\n  }\n\n  getFeatures(\n    region: Region & { originalRefName?: string },\n    opts?: BaseOptions & {\n      filterBy: IFilter\n    },\n  ) {\n    const { refName, start, end, originalRefName } = region\n    const { signal, filterBy, statusCallback = () => {} } = opts || {}\n    return ObservableCreate<Feature>(async observer => {\n      const { bam } = await this.configure()\n      await this.setup(opts)\n      const records = await updateStatus(\n        'Downloading alignments',\n        statusCallback,\n        () => bam.getRecordsForRange(refName, start, end, opts),\n      )\n\n      await updateStatus('Processing alignments', statusCallback, async () => {\n        const {\n          flagInclude = 0,\n          flagExclude = 0,\n          tagFilter,\n          readName,\n        } = filterBy || {}\n\n        for (const record of records) {\n          let ref: string | undefined\n          if (!record.get('MD')) {\n            ref = await this.seqFetch(\n              originalRefName || refName,\n              record.get('start'),\n              record.get('end'),\n            )\n          }\n\n          const flags = record.flags\n          if ((flags & flagInclude) !== flagInclude && !(flags & flagExclude)) {\n            continue\n          }\n\n          if (tagFilter) {\n            const readVal = record.get(tagFilter.tag)\n            const filterVal = tagFilter.value\n            if (\n              filterVal === '*'\n                ? readVal !== undefined\n                : `${readVal}` !== `${filterVal}`\n            ) {\n              continue\n            }\n          }\n\n          if (readName && record.get('name') !== readName) {\n            continue\n          }\n\n          observer.next(new BamSlightlyLazyFeature(record, this, ref))\n        }\n        observer.complete()\n      })\n    }, signal)\n  }\n\n  async getMultiRegionFeatureDensityStats(\n    regions: Region[],\n    opts?: BaseOptions,\n  ) {\n    const { bam } = await this.configure()\n    // this is a method to avoid calling on htsget adapters\n    if (bam.index) {\n      const bytes = await bytesForRegions(regions, bam)\n      const fetchSizeLimit = this.getConf('fetchSizeLimit')\n      return { bytes, fetchSizeLimit }\n    }\n    return super.getMultiRegionFeatureDensityStats(regions, opts)\n  }\n\n  freeResources(/* { region } */): void {}\n\n  // depends on setup being called before the BAM constructor\n  refIdToName(refId: number) {\n    return this.samHeader?.idToName[refId]\n  }\n}\n"],"names":["BamSlightlyLazyFeature","constructor","record","adapter","ref","_get_name","this","get","_get_type","_get_score","_get_flags","flags","_get_strand","isReverseComplemented","_get_pair_orientation","isPaired","getPairOrientation","undefined","_get_next_ref","refIdToName","_next_refid","_get_next_pos","_next_pos","_get_next_segment_position","_get_seq","getReadBases","qualRaw","set","tags","properties","Object","getOwnPropertyNames","prototype","Set","filter","prop","startsWith","map","methodName","replace","concat","_tags","id","field","_get_refName","seq_id","parent","children","pairedFeature","toJSON","fromEntries","t","elt","uniqueId","_get_mismatches","getMismatches","_get_clipPos","cigar","getClip","BamAdapter","BaseFeatureDataAdapter","configurePre","bamLocation","getConf","location","indexType","pm","pluginManager","csi","bam","BamFile","bamFilehandle","openLocation","csiFilehandle","baiFilehandle","yieldThreadTime","Number","POSITIVE_INFINITY","adapterConfig","getSubAdapter","dataAdapter","sequenceAdapter","configure","configureP","catch","e","getHeader","opts","getHeaderText","setupPre","statusCallback","samHeader","updateStatus","async","idToName","nameToId","l","tag","forEach","sqLine","refId","SN","data","find","item","refName","value","setup","setupP","getRefNames","seqFetch","start","end","features","getFeatures","assemblyName","seqChunks","firstValueFrom","pipe","toArray","sequence","sort","a","b","chunk","chunkStart","chunkEnd","trimStart","Math","max","trimLength","min","chunkSeq","slice","length","Error","toLocaleString","region","originalRefName","signal","filterBy","ObservableCreate","records","getRecordsForRange","flagInclude","flagExclude","tagFilter","readName","readVal","filterVal","observer","next","complete","getMultiRegionFeatureDensityStats","regions","index","bytes","bytesForRegions","fetchSizeLimit","super","freeResources"],"sourceRoot":""}