Mercurial > repos > fubar > jbrowse2
view autogenJB2.py @ 51:e18f2d6c4dbe draft
planemo upload for repository https://github.com/usegalaxy-eu/temporary-tools/tree/master/jbrowse2 commit 44df75b3714aa9e02983e0b67ef43fc0eee4a8d4
author | fubar |
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date | Thu, 07 Mar 2024 04:52:28 +0000 |
parents | 460d5b6c5d98 |
children | ae12977c0e5e |
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import argparse import logging import os import sys from jbrowse2 import JbrowseConnector as jbC logging.basicConfig(level=logging.debug) log = logging.getLogger("jbrowse") def makeDefaultLocation(): refName = jc.genome_firstcontig defloc = "%s:100..10000" % refName return defloc if __name__ == "__main__": parser = argparse.ArgumentParser(description="", epilog="") parser.add_argument("--sessName", help="Session name", default="AutoJBrowse") parser.add_argument( "--trackmeta", help="Repeatable 'filename,filext,filepath,[bai/crai path for filesystem bam/cram]' for JBrowse2 tracks", default=[], action="append", ) parser.add_argument( "--referencemeta", help="Repeatable 'filename, filext, filepath, ... ,' for JBrowse2 reference tracks - usually only one needed", default=[], action="append", ) parser.add_argument( "--pafmeta", help="Repeatable. Each is a 'pafname, filext, filepath, ... ,' for a JBrowse2 paf track", default=[], action="append", ) parser.add_argument( "--pafreferencemeta", help="Repeatable. Each is a 'pafname,refpath,refname' Every pafname must have one or more", default=[], action="append", ) parser.add_argument( "--jbrowse2path", help="Path to JBrowse2 directory in biocontainer or Conda" ) parser.add_argument("--outdir", help="Output directory", required=True) parser.add_argument("--version", "-V", action="version", version="%(prog)s 2.10.2") args = parser.parse_args() sessName = args.sessName # --trackmeta $jbrowseme[$key],$jbrowseme[$key].ext,'$key' trackList = [x.strip().split(",") for x in args.trackmeta if x > ""] refList = [x.strip().split(",") for x in args.referencemeta if x > ""] if len(refList) > 0: listgenomes = [f for f in refList if f[1] in ["fasta", "fasta.gz"]] # assume no pafs here if len(listgenomes) > 0: genome_paths = [x[0] for x in listgenomes] genome_names = [x[2] for x in listgenomes] guseuri = [] for x in genome_paths: if x.startswith('http://') or x.startswith('https://'): guseuri.append('yes') else: guseuri.append('no') jc = jbC( outdir=args.outdir, jbrowse2path=args.jbrowse2path, genomes=[ { "path": x, "label": genome_names[i], "useuri": guseuri[i], "meta": {"name": genome_names[i], "dataset_dname": genome_names[i] } } for i, x in enumerate(genome_paths) ], ) jc.process_genomes() default_session_data = { "visibility": { "default_on": [], "default_off": [], }, "style": {}, "style_labels": {}, } track_conf = {} listtracks = trackList # foo.paf must have a foo_paf.fasta or fasta.gz to match tnames = [x[2] for x in listtracks] texts = [x[1] for x in listtracks] for i, track in enumerate(listtracks): tpath, trext, trackname = track[:3] track_conf["dataset_id"] = trackname if trext == "paf": refname = trackname + "_paf.fasta" refdat = [x[2] for x in listtracks if x[2] == refname] if not refdat: jc.logging.warn( "!! No reference file %s corresponding to paf file %s found. Not building - there must be a corresponding fasta for each paf" % (refname, trackname) ) sys.exit(3) else: track_conf.update({ "conf": { "options": { "paf": {"genome": refdat, "genome_label": trackname} } } }) elif trext == "bam": ipath = track[3] if not os.path.exists(ipath): ipath = os.path.realpath(os.path.join(jc.outdir, trackname + '.bai')) cmd = ["samtools", "index", "-b", "-o", ipath, os.path.realpath(track[0])] sys.stdout.write('#### calling %s' % ' '.join(cmd)) jc.subprocess_check_call(cmd) track_conf.update({"conf": {"options": {"bam": {"bam_index": ipath}}}}) elif trext == "cram": ipath = track[3] if not os.path.exists(ipath): jc.logging.info('calling %s' % ' '.join(cmd)) ipath = os.path.realpath(os.path.join('./', trackname + '.crai')) cmd = ["samtools", "index", "-c", "-o", ipath, os.path.realpath(track[0])] sys.stdout.write('#### calling %s' % ' '.join(cmd)) jc.subprocess_check_call(cmd) track_conf.update({"conf": {"options": {"cram": {"cram_index": ipath}}}}) track_conf["path"] = tpath track_conf["format"] = trext track_conf["name"] = trackname track_conf["label"] = trackname useu = tpath.startswith('http://') or tpath.startswith('https://') useuri = 'no' if useu: useuri = 'yes' track_conf["trackfiles"] = [ (tpath, trext, useuri, trackname, {}), ] track_conf["category"] = "Autogenerated" keys = jc.process_annotations(track_conf) if keys: for key in keys: if trext in [ "bigwig", "gff3", "gff", "vcf", "maf", ]: default_session_data["visibility"]["default_on"].append(key) else: default_session_data["visibility"]["default_off"].append( key ) # general_data = { # "analytics": root.find("metadata/general/analytics").text, # "primary_color": root.find("metadata/general/primary_color").text, # "secondary_color": root.find("metadata/general/secondary_color").text, # "tertiary_color": root.find("metadata/general/tertiary_color").text, # "quaternary_color": root.find("metadata/general/quaternary_color").text, # "font_size": root.find("metadata/general/font_size").text, # } # jc.add_general_configuration(general_data) trackconf = jc.config_json.get("tracks", None) if trackconf: jc.config_json["tracks"].update(jc.tracksToAdd) else: jc.config_json["tracks"] = jc.tracksToAdd jc.write_config() defLoc = makeDefaultLocation() default_session_data.update( {"defaultLocation": defLoc, "session_name": sessName} ) track_conf.update(default_session_data) jc.add_default_session(default_session_data) # jc.text_index() not sure what broke here. else: sys.stderr.write( "!!!! Collection has no suitable trackfiles for autogenJB2 - nothing to process" )