Mercurial > repos > fubar > tool_factory_2
comparison README.txt @ 22:4e3aa95ed3ac draft
Uploaded
author | fubar |
---|---|
date | Mon, 02 Mar 2015 05:14:05 -0500 |
parents | d98f5a09137f |
children |
comparison
equal
deleted
inserted
replaced
21:71b85c322600 | 22:4e3aa95ed3ac |
---|---|
1 # WARNING before you start | 1 # WARNING before you start |
2 # Install this tool on a private Galaxy ONLY | 2 # Install this tool on a private Galaxy ONLY |
3 # Please NEVER on a public or production instance | 3 # Please NEVER on a public or production instance |
4 # updated august 2014 by John Chilton adding citation support | 4 # updated august 2014 by John Chilton adding citation support |
5 # | 5 # |
6 # updated august 8 2014 to fix bugs reported by Marius van den Beek | 6 # updated august 8 2014 to fix bugs reported by Marius van den Beek |
7 # please cite the resource at | 7 # please cite the resource at http://bioinformatics.oxfordjournals.org/cgi/reprint/bts573?ijkey=lczQh1sWrMwdYWJ&keytype=ref |
8 http://bioinformatics.oxfordjournals.org/cgi/reprint/bts573?ijkey=lczQh1sWrMwdYWJ&keytype=ref | |
9 # if you use this tool in your published work. | 8 # if you use this tool in your published work. |
10 | 9 |
11 *Short Story* | 10 *Short Story* |
12 | 11 |
13 This is an unusual Galaxy tool capable of generating new Galaxy tools. | 12 This is an unusual Galaxy tool capable of generating new Galaxy tools. |
14 It works by exposing *unrestricted* and therefore extremely dangerous scripting | 13 It works by exposing *unrestricted* and therefore extremely dangerous |
15 to all designated administrators of the host Galaxy server, allowing them to | 14 scripting to all designated administrators of the host Galaxy server, allowing them to run scripts |
16 run scripts in R, python, sh and perl over multiple selected input data sets, | 15 in R, python, sh and perl over multiple selected input data sets, writing a single new data set as output. |
17 writing a single new data set as output. | |
18 | |
19 *Differences between TF2 and the original Tool Factory* | |
20 | |
21 1. TF2 (this one) allows any number of either fixed or user-editable parameters to be defined | |
22 for the new tool. If these are editable, the user can change them but otherwise, they are passed | |
23 as fixed and invisible parameters for each execution. Obviously, there are substantial security | |
24 implications with editable parameters, but these are always sanitized by Galaxy's inbuilt | |
25 parameter sanitization so you may need to "unsanitize" characters - eg translate all "__lt__" | |
26 into "<" for certain parameters where that is needed. Please practise safe toolshed. | |
27 | |
28 2. Any number of (the same datatype) of input files may be defined. | |
29 | |
30 These changes substantially complicate the way your supplied script is supplied with | |
31 all the new and variable parameters. Examples in each scripting language are shown | |
32 in the tool help | |
33 | 16 |
34 *Automated outputs in named sections* | 17 *Automated outputs in named sections* |
35 | 18 |
36 If your script writes to the current directory path, arbitrary mix of (eg) | 19 If your script writes to the current directory path, arbitrary mix of (eg) pdfs, tabular analysis results and run logs, |
37 pdfs, tabular analysis results and run logs,the tool factory can optionally | 20 the tool factory can optionally auto-generate a linked Html page with separate sections showing a thumbnail grid |
38 auto-generate a linked Html page with separate sections showing a thumbnail | 21 for all pdfs and the log text, grouping all artifacts sharing a file name and log name prefix:: |
39 grid for all pdfs and the log text, grouping all artifacts sharing a file | 22 |
40 name and log name prefix:: | 23 eg: if "foo.log" is emitted then *all* other outputs matching foo_* will all be grouped together - eg |
41 | |
42 eg: if "foo.log" is emitted then *all* other outputs matching foo_* will | |
43 all be grouped together - eg | |
44 foo_baz.pdf | 24 foo_baz.pdf |
45 foo_bar.pdf and | 25 foo_bar.pdf and |
46 foo_zot.xls | 26 foo_zot.xls |
47 would all be displayed and linked in the same section with foo.log's contents | 27 would all be displayed and linked in the same section with foo.log's contents - to form the "Foo" section of the Html page. |
48 - to form the "Foo" section of the Html page. Sections appear in alphabetic | 28 Sections appear in alphabetic order and there are no limits on the number of files or sections. |
49 order and there are no limits on the number of files or sections. | 29 |
50 | 30 *Automated generation of new Galaxy tool shed tools for installation into any Galaxy* |
51 *Automated generation of new Galaxy tools for installation into any Galaxy* | 31 |
52 | 32 Once a script is working correctly, this tool optionally generates a new Galaxy tool, effectively |
53 Once a script is working correctly, this tool optionally generates a | 33 freezing the supplied script into a new, ordinary Galaxy tool that runs it over one or more input files |
54 new Galaxy tool, effectively freezing the supplied script into a new, | 34 selected by the user. Generated tools are installed via a tool shed by an administrator and work exactly like all other Galaxy tools for your users. |
55 ordinary Galaxy tool that runs it over one or more input files selected by | 35 |
56 the user. Generated tools are installed via a tool shed by an administrator | 36 If you use the Html output option, please ensure that sanitize_all_html is set to False and |
57 and work exactly like all other Galaxy tools for your users. | 37 uncommented in universe_wsgi.ini - it should show:: |
58 | |
59 If you use the Html output option, please ensure that sanitize_all_html is | |
60 set to False and uncommented in universe_wsgi.ini - it should show:: | |
61 | 38 |
62 # By default, all tool output served as 'text/html' will be sanitized | 39 # By default, all tool output served as 'text/html' will be sanitized |
63 sanitize_all_html = False | 40 sanitize_all_html = False |
64 | 41 |
65 This opens potential security risks and may not be acceptable for public | 42 This opens potential security risks and may not be acceptable for public sites where the lack of stylesheets |
66 sites where the lack of stylesheets may make Html pages damage onlookers' | 43 may make Html pages damage onlookers' eyeballs but should still be correct. |
67 eyeballs but should still be correct. | |
68 | 44 |
69 | 45 |
70 *More Detail* | 46 *More Detail* |
71 | 47 |
72 To use the ToolFactory, you should have prepared a script to paste into a | 48 To use the ToolFactory, you should have prepared a script to paste into a text box, |
73 text box, and a small test input example ready to select from your history | 49 and a small test input example ready to select from your history to test your new script. |
74 to test your new script. | 50 There is an example in each scripting language on the Tool Factory form. You can just |
75 | 51 cut and paste these to try it out - remember to select the right interpreter please. You'll |
76 There is an example in each scripting language on the Tool Factory form. You | 52 also need to create a small test data set using the Galaxy history add new data tool. |
77 can just cut and paste these to try it out - remember to select the right | 53 |
78 interpreter please. You'll also need to create a small test data set using | 54 If the script fails somehow, use the "redo" button on the tool output in your history to |
79 the Galaxy history add new data tool. | 55 recreate the form complete with broken script. Fix the bug and execute again. Rinse, wash, repeat. |
80 | 56 |
81 If the script fails somehow, use the "redo" button on the tool output in | 57 Once the script runs sucessfully, a new Galaxy tool that runs your script can be generated. |
82 your history to recreate the form complete with broken script. Fix the bug | 58 Select the "generate" option and supply some help text and names. The new tool will be |
83 and execute again. Rinse, wash, repeat. | 59 generated in the form of a new Galaxy datatype - toolshed.gz - as the name suggests, |
84 | 60 it's an archive ready to upload to a Galaxy ToolShed as a new tool repository. |
85 Once the script runs sucessfully, a new Galaxy tool that runs your script | 61 |
86 can be generated. Select the "generate" option and supply some help text and | 62 Once it's in a ToolShed, it can be installed into any local Galaxy server from |
87 names. The new tool will be generated in the form of a new Galaxy datatype | 63 the server administrative interface. |
88 - toolshed.gz - as the name suggests, it's an archive ready to upload to a | 64 |
89 Galaxy ToolShed as a new tool repository. | 65 Once the new tool is installed, local users can run it - each time, the script that was supplied |
90 | 66 when it was built will be executed with the input chosen from the user's history. In other words, |
91 Once it's in a ToolShed, it can be installed into any local Galaxy server | 67 the tools you generate with the ToolFactory run just like any other Galaxy tool, |
92 from the server administrative interface. | 68 but run your script every time. |
93 | 69 |
94 Once the new tool is installed, local users can run it - each time, the script | 70 Tool factory tools are perfect for workflow components. One input, one output, no variables. |
95 that was supplied when it was built will be executed with the input chosen | 71 |
96 from the user's history. In other words, the tools you generate with the | 72 *To fully and safely exploit the awesome power* of this tool, Galaxy and the ToolShed, |
97 ToolFactory run just like any other Galaxy tool,but run your script every time. | 73 you should be a developer installing this tool on a private/personal/scratch local instance where you |
98 | 74 are an admin_user. Then, if you break it, you get to keep all the pieces |
99 Tool factory tools are perfect for workflow components. One input, one output, | 75 see https://bitbucket.org/fubar/galaxytoolfactory/wiki/Home |
100 no variables. | |
101 | |
102 *To fully and safely exploit the awesome power* of this tool, | |
103 Galaxy and the ToolShed, you should be a developer installing this | |
104 tool on a private/personal/scratch local instance where you are an | |
105 admin_user. Then, if you break it, you get to keep all the pieces see | |
106 https://bitbucket.org/fubar/galaxytoolfactory/wiki/Home | |
107 | 76 |
108 ** Installation ** | 77 ** Installation ** |
109 This is a Galaxy tool. You can install it most conveniently using the | 78 This is a Galaxy tool. You can install it most conveniently using the administrative "Search and browse tool sheds" link. |
110 administrative "Search and browse tool sheds" link. Find the Galaxy Main | 79 Find the Galaxy Main toolshed at https://toolshed.g2.bx.psu.edu/ and search for the toolfactory repository. |
111 toolshed at https://toolshed.g2.bx.psu.edu/ and search for the toolfactory | 80 Open it and review the code and select the option to install it. |
112 repository. Open it and review the code and select the option to install it. | |
113 | 81 |
114 ( | 82 ( |
115 If you can't get the tool that way, the xml and py files here need to be | 83 If you can't get the tool that way, the xml and py files here need to be copied into a new tools |
116 copied into a new tools | 84 subdirectory such as tools/toolfactory Your tool_conf.xml needs a new entry pointing to the xml |
117 subdirectory such as tools/toolfactory Your tool_conf.xml needs a new entry | |
118 pointing to the xml | |
119 file - something like:: | 85 file - something like:: |
120 | 86 |
121 <section name="Tool building tools" id="toolbuilders"> | 87 <section name="Tool building tools" id="toolbuilders"> |
122 <tool file="toolfactory/rgToolFactory.xml"/> | 88 <tool file="toolfactory/rgToolFactory.xml"/> |
123 </section> | 89 </section> |
124 | 90 |
125 If not already there (I just added it to datatypes_conf.xml.sample), | 91 If not already there (I just added it to datatypes_conf.xml.sample), please add: |
126 please add: | 92 <datatype extension="toolshed.gz" type="galaxy.datatypes.binary:Binary" mimetype="multipart/x-gzip" subclass="True" /> |
127 <datatype extension="toolshed.gz" type="galaxy.datatypes.binary:Binary" | |
128 mimetype="multipart/x-gzip" subclass="True" /> | |
129 to your local data_types_conf.xml. | 93 to your local data_types_conf.xml. |
130 ) | 94 ) |
131 | 95 |
132 Of course, R, python, perl etc are needed on your path if you want to test | 96 Of course, R, python, perl etc are needed on your path if you want to test scripts using those interpreters. |
133 scripts using those interpreters. Adding new ones to this tool code should | 97 Adding new ones to this tool code should be easy enough. Please make suggestions as bitbucket issues and code. |
134 be easy enough. Please make suggestions as bitbucket issues and code. The | 98 The HTML file code automatically shrinks R's bloated pdfs, and depends on ghostscript. The thumbnails require imagemagick . |
135 HTML file code automatically shrinks R's bloated pdfs, and depends on | |
136 ghostscript. The thumbnails require imagemagick . | |
137 | 99 |
138 * Restricted execution * | 100 * Restricted execution * |
139 The tool factory tool itself will then be usable ONLY by admin users - | 101 The tool factory tool itself will then be usable ONLY by admin users - people with IDs in admin_users in universe_wsgi.ini |
140 people with IDs in admin_users in universe_wsgi.ini **Yes, that's right. ONLY | 102 **Yes, that's right. ONLY admin_users can run this tool** Think about it for a moment. If allowed to run any |
141 admin_users can run this tool** Think about it for a moment. If allowed to | 103 arbitrary script on your Galaxy server, the only thing that would impede a miscreant bent on destroying all your |
142 run any arbitrary script on your Galaxy server, the only thing that would | 104 Galaxy data would probably be lack of appropriate technical skills. |
143 impede a miscreant bent on destroying all your Galaxy data would probably | 105 |
144 be lack of appropriate technical skills. | 106 *What it does* This is a tool factory for simple scripts in python, R and perl currently. |
145 | 107 Functional tests are automatically generated. How cool is that. |
146 *What it does* This is a tool factory for simple scripts in python, R and | 108 |
147 perl currently. Functional tests are automatically generated. How cool is that. | 109 LIMITED to simple scripts that read one input from the history. |
148 | 110 Optionally can write one new history dataset, |
149 LIMITED to simple scripts that read one input from the history. Optionally can | 111 and optionally collect any number of outputs into links on an autogenerated HTML |
150 write one new history dataset, and optionally collect any number of outputs | 112 index page for the user to navigate - useful if the script writes images and output files - pdf outputs |
151 into links on an autogenerated HTML index page for the user to navigate - | 113 are shown as thumbnails and R's bloated pdf's are shrunk with ghostscript so that and imagemagik need to |
152 useful if the script writes images and output files - pdf outputs are shown | 114 be avaailable. |
153 as thumbnails and R's bloated pdf's are shrunk with ghostscript so that and | 115 |
154 imagemagik need to be available. | 116 Generated tools can be edited and enhanced like any Galaxy tool, so start small and build up since |
155 | 117 a generated script gets you a serious leg up to a more complex one. |
156 Generated tools can be edited and enhanced like any Galaxy tool, so start | 118 |
157 small and build up since a generated script gets you a serious leg up to a | 119 *What you do* You paste and run your script |
158 more complex one. | 120 you fix the syntax errors and eventually it runs |
159 | 121 You can use the redo button and edit the script before |
160 *What you do* You paste and run your script, you fix the syntax errors and | |
161 eventually it runs. You can use the redo button and edit the script before | |
162 trying to rerun it as you debug - it works pretty well. | 122 trying to rerun it as you debug - it works pretty well. |
163 | 123 |
164 Once the script works on some test data, you can generate a toolshed compatible | 124 Once the script works on some test data, you can |
165 gzip file containing your script ready to run as an ordinary Galaxy tool in | 125 generate a toolshed compatible gzip file |
166 a repository on your local toolshed. That means safe and largely automated | 126 containing your script ready to run as an ordinary Galaxy tool in a |
167 installation in any production Galaxy configured to use your toolshed. | 127 repository on your local toolshed. That means safe and largely automated installation in any |
128 production Galaxy configured to use your toolshed. | |
168 | 129 |
169 *Generated tool Security* Once you install a generated tool, it's just | 130 *Generated tool Security* Once you install a generated tool, it's just |
170 another tool - assuming the script is safe. They just run normally and their | 131 another tool - assuming the script is safe. They just run normally and their user cannot do anything unusually insecure |
171 user cannot do anything unusually insecure but please, practice safe toolshed. | 132 but please, practice safe toolshed. |
172 Read the fucking code before you install any tool. Especially this one - | 133 Read the fucking code before you install any tool. |
173 it is really scary. | 134 Especially this one - it is really scary. |
174 | 135 |
175 If you opt for an HTML output, you get all the script outputs arranged | 136 If you opt for an HTML output, you get all the script outputs arranged |
176 as a single Html history item - all output files are linked, thumbnails for | 137 as a single Html history item - all output files are linked, thumbnails for all the pdfs. |
177 all the pdfs. Ugly but really inexpensive. | 138 Ugly but really inexpensive. |
178 | 139 |
179 Patches and suggestions welcome as bitbucket issues please? | 140 Patches and suggestions welcome as bitbucket issues please? |
180 | 141 |
181 copyright ross lazarus (ross stop lazarus at gmail stop com) May 2012 | 142 copyright ross lazarus (ross stop lazarus at gmail stop com) May 2012 |
182 | 143 |
183 all rights reserved | 144 all rights reserved |
184 Licensed under the LGPL if you want to improve it, feel free | 145 Licensed under the LGPL if you want to improve it, feel free https://bitbucket.org/fubar/galaxytoolfactory/wiki/Home |
185 https://bitbucket.org/fubar/galaxytoolfactory/wiki/Home | 146 |
186 | 147 Material for our more enthusiastic and voracious readers continues below - we salute you. |
187 Material for our more enthusiastic and voracious readers continues below - | 148 |
188 we salute you. | 149 **Motivation** Simple transformation, filtering or reporting scripts get written, run and lost every day in most busy labs |
189 | 150 - even ours where Galaxy is in use. This 'dark script matter' is pervasive and generally not reproducible. |
190 **Motivation** Simple transformation, filtering or reporting scripts get | 151 |
191 written, run and lost every day in most busy labs - even ours where Galaxy is | 152 **Benefits** For our group, this allows Galaxy to fill that important dark script gap - all those "small" bioinformatics |
192 in use. This 'dark script matter' is pervasive and generally not reproducible. | 153 tasks. Once a user has a working R (or python or perl) script that does something Galaxy cannot currently do (eg transpose a |
193 | 154 tabular file) and takes parameters the way Galaxy supplies them (see example below), they: |
194 **Benefits** For our group, this allows Galaxy to fill that important dark | |
195 script gap - all those "small" bioinformatics tasks. Once a user has a working | |
196 R (or python or perl) script that does something Galaxy cannot currently do | |
197 (eg transpose a tabular file) and takes parameters the way Galaxy supplies | |
198 them (see example below), they: | |
199 | 155 |
200 1. Install the tool factory on a personal private instance | 156 1. Install the tool factory on a personal private instance |
201 | 157 |
202 2. Upload a small test data set | 158 2. Upload a small test data set |
203 | 159 |
204 3. Paste the script into the 'script' text box and iteratively run the | 160 3. Paste the script into the 'script' text box and iteratively run the insecure tool on test data until it works right - |
205 insecure tool on test data until it works right - there is absolutely no | 161 there is absolutely no reason to do this anywhere other than on a personal private instance. |
206 reason to do this anywhere other than on a personal private instance. | 162 |
207 | 163 4. Once it works right, set the 'Generate toolshed gzip' option and run it again. |
208 4. Once it works right, set the 'Generate toolshed gzip' option and run | 164 |
209 it again. | 165 5. A toolshed style gzip appears ready to upload and install like any other Toolshed entry. |
210 | |
211 5. A toolshed style gzip appears ready to upload and install like any other | |
212 Toolshed entry. | |
213 | 166 |
214 6. Upload the new tool to the toolshed | 167 6. Upload the new tool to the toolshed |
215 | 168 |
216 7. Ask the local admin to check the new tool to confirm it's not evil and | 169 7. Ask the local admin to check the new tool to confirm it's not evil and install it in the local production galaxy |
217 install it in the local production galaxy | |
218 | 170 |
219 **Simple examples on the tool form** | 171 **Simple examples on the tool form** |
220 | 172 |
221 A simple Rscript "filter" showing how the command line parameters can be | 173 A simple Rscript "filter" showing how the command line parameters can be handled, takes an input file, |
222 handled, takes an input file, does something (transpose in this case) and | 174 does something (transpose in this case) and writes the results to a new tabular file:: |
223 writes the results to a new tabular file:: | |
224 | 175 |
225 # transpose a tabular input file and write as a tabular output file | 176 # transpose a tabular input file and write as a tabular output file |
226 ourargs = commandArgs(TRUE) | 177 ourargs = commandArgs(TRUE) |
227 inf = ourargs[1] | 178 inf = ourargs[1] |
228 outf = ourargs[2] | 179 outf = ourargs[2] |
229 inp = read.table(inf,head=F,row.names=NULL,sep='\t') | 180 inp = read.table(inf,head=F,row.names=NULL,sep='\t') |
230 outp = t(inp) | 181 outp = t(inp) |
231 write.table(outp,outf, quote=FALSE, sep="\t",row.names=F,col.names=F) | 182 write.table(outp,outf, quote=FALSE, sep="\t",row.names=F,col.names=F) |
232 | 183 |
233 Calculate a multiple test adjusted p value from a column of p values - | 184 Calculate a multiple test adjusted p value from a column of p values - for this script to be useful, |
234 for this script to be useful, it needs the right column for the input to be | 185 it needs the right column for the input to be specified in the code for the |
235 specified in the code for the given input file type(s) specified when the | 186 given input file type(s) specified when the tool is generated :: |
236 tool is generated :: | 187 |
237 | 188 # use p.adjust - assumes a HEADER row and column 1 - please fix for any real use |
238 # use p.adjust - assumes a HEADER row and column 1 - please fix for any | |
239 real use | |
240 column = 1 # adjust if necessary for some other kind of input | 189 column = 1 # adjust if necessary for some other kind of input |
241 fdrmeth = 'BH' | 190 fdrmeth = 'BH' |
242 ourargs = commandArgs(TRUE) | 191 ourargs = commandArgs(TRUE) |
243 inf = ourargs[1] | 192 inf = ourargs[1] |
244 outf = ourargs[2] | 193 outf = ourargs[2] |
247 q = p.adjust(p,method=fdrmeth) | 196 q = p.adjust(p,method=fdrmeth) |
248 newval = paste(fdrmeth,'p-value',sep='_') | 197 newval = paste(fdrmeth,'p-value',sep='_') |
249 q = data.frame(q) | 198 q = data.frame(q) |
250 names(q) = newval | 199 names(q) = newval |
251 outp = cbind(inp,newval=q) | 200 outp = cbind(inp,newval=q) |
252 write.table(outp,outf, quote=FALSE, sep="\t",row.names=F,col.names=T) | 201 write.table(outp,outf, quote=FALSE, sep="\t",row.names=F,col.names=T) |
253 | 202 |
254 | 203 |
255 | 204 |
256 Another Rscript example without any input file - generates a random heatmap | 205 Another Rscript example without any input file - generates a random heatmap pdf - you must make sure the option to create an HTML output file is |
257 pdf - you must make sure the option to create an HTML output file is | 206 turned on for this to work. The heatmap will be presented as a thumbnail linked to the pdf in the resulting HTML page:: |
258 turned on for this to work. The heatmap will be presented as a thumbnail | |
259 linked to the pdf in the resulting HTML page:: | |
260 | 207 |
261 # note this script takes NO input or output because it generates random data | 208 # note this script takes NO input or output because it generates random data |
262 foo = data.frame(a=runif(100),b=runif(100),c=runif(100),d=runif(100), | 209 foo = data.frame(a=runif(100),b=runif(100),c=runif(100),d=runif(100),e=runif(100),f=runif(100)) |
263 e=runif(100),f=runif(100)) | |
264 bar = as.matrix(foo) | 210 bar = as.matrix(foo) |
265 pdf( "heattest.pdf" ) | 211 pdf( "heattest.pdf" ) |
266 heatmap(bar,main='Random Heatmap') | 212 heatmap(bar,main='Random Heatmap') |
267 dev.off() | 213 dev.off() |
268 | 214 |
269 A Python example that reverses each row of a tabular file. You'll need | 215 A Python example that reverses each row of a tabular file. You'll need to remove the leading spaces for this to work if cut |
270 to remove the leading spaces for this to work if cut and pasted into the | 216 and pasted into the script box. Note that you can already do this in Galaxy by setting up the cut columns tool with the |
271 script box. Note that you can already do this in Galaxy by setting up the | 217 correct number of columns in reverse order,but this script will work for any number of columns so is completely generic:: |
272 cut columns tool with the correct number of columns in reverse order,but | |
273 this script will work for any number of columns so is completely generic:: | |
274 | 218 |
275 # reverse order of columns in a tabular file | 219 # reverse order of columns in a tabular file |
276 import sys | 220 import sys |
277 inp = sys.argv[1] | 221 inp = sys.argv[1] |
278 outp = sys.argv[2] | 222 outp = sys.argv[2] |
286 i.close() | 230 i.close() |
287 o.close() | 231 o.close() |
288 | 232 |
289 | 233 |
290 Galaxy as an IDE for developing API scripts | 234 Galaxy as an IDE for developing API scripts |
291 If you need to develop Galaxy API scripts and you like to live dangerously, | 235 If you need to develop Galaxy API scripts and you like to live dangerously, please read on. |
292 please read on. | |
293 | 236 |
294 Galaxy as an IDE? | 237 Galaxy as an IDE? |
295 Amazingly enough, blend-lib API scripts run perfectly well *inside* | 238 Amazingly enough, blend-lib API scripts run perfectly well *inside* Galaxy when pasted into a Tool Factory form. No need to generate a new tool. Galaxy+Tool_Factory = IDE I think we need a new t-shirt. Seriously, it is actually quite useable. |
296 Galaxy when pasted into a Tool Factory form. No need to generate a new | |
297 tool. Galaxy+Tool_Factory = IDE I think we need a new t-shirt. Seriously, | |
298 it is actually quite useable. | |
299 | 239 |
300 Why bother - what's wrong with Eclipse | 240 Why bother - what's wrong with Eclipse |
301 Nothing. But, compared with developing API scripts in the usual way outside | 241 Nothing. But, compared with developing API scripts in the usual way outside Galaxy, you get persistence and other framework benefits plus at absolutely no extra charge, a ginormous security problem if you share the history or any outputs because they contain the api script with key so development servers only please! |
302 Galaxy, you get persistence and other framework benefits plus at absolutely | |
303 no extra charge, a ginormous security problem if you share the history or | |
304 any outputs because they contain the api script with key so development | |
305 servers only please! | |
306 | 242 |
307 Workflow | 243 Workflow |
308 Fire up the Tool Factory in Galaxy. | 244 Fire up the Tool Factory in Galaxy. |
309 | 245 |
310 Leave the input box empty, set the interpreter to python, paste and run an | 246 Leave the input box empty, set the interpreter to python, paste and run an api script - eg working example (substitute the url and key) below. |
311 api script - eg working example (substitute the url and key) below. | 247 |
312 | 248 It took me a few iterations to develop the example below because I know almost nothing about the API. I started with very simple code from one of the samples and after each run, the (edited..) api script is conveniently recreated using the redo button on the history output item. So each successive version of the developing api script you run is persisted - ready to be edited and rerun easily. It is ''very'' handy to be able to add a line of code to the script and run it, then view the output to (eg) inspect dicts returned by API calls to help move progressively deeper iteratively. |
313 It took me a few iterations to develop the example below because I know | 249 |
314 almost nothing about the API. I started with very simple code from one of the | 250 Give the below a whirl on a private clone (install the tool factory from the main toolshed) and try adding complexity with few rerun/edit/rerun cycles. |
315 samples and after each run, the (edited..) api script is conveniently recreated | |
316 using the redo button on the history output item. So each successive version | |
317 of the developing api script you run is persisted - ready to be edited and | |
318 rerun easily. It is ''very'' handy to be able to add a line of code to the | |
319 script and run it, then view the output to (eg) inspect dicts returned by | |
320 API calls to help move progressively deeper iteratively. | |
321 | |
322 Give the below a whirl on a private clone (install the tool factory from | |
323 the main toolshed) and try adding complexity with few rerun/edit/rerun cycles. | |
324 | 251 |
325 Eg tool factory api script | 252 Eg tool factory api script |
326 import sys | 253 import sys |
327 from blend.galaxy import GalaxyInstance | 254 from blend.galaxy import GalaxyInstance |
328 ourGal = 'http://x.x.x.x:xxxx' | 255 ourGal = 'http://x.x.x.x:xxxx' |
329 ourKey = 'xxx' | 256 ourKey = 'xxx' |
330 gi = GalaxyInstance(ourGal, key=ourKey) | 257 gi = GalaxyInstance(ourGal, key=ourKey) |
331 libs = gi.libraries.get_libraries() | 258 libs = gi.libraries.get_libraries() |
332 res = [] | 259 res = [] |
333 # libs looks like | 260 # libs looks like |
334 # u'url': u'/galaxy/api/libraries/441d8112651dc2f3', u'id': | 261 # u'url': u'/galaxy/api/libraries/441d8112651dc2f3', u'id': u'441d8112651dc2f3', u'name':.... u'Demonstration sample RNA data', |
335 u'441d8112651dc2f3', u'name':.... u'Demonstration sample RNA data', | 262 for lib in libs: |
336 for lib in libs: | |
337 res.append('%s:\n' % lib['name']) | 263 res.append('%s:\n' % lib['name']) |
338 res.append(str(gi.libraries.show_library(lib['id'],contents=True))) | 264 res.append(str(gi.libraries.show_library(lib['id'],contents=True))) |
339 outf=open(sys.argv[2],'w') | 265 outf=open(sys.argv[2],'w') |
340 outf.write('\n'.join(res)) | 266 outf.write('\n'.join(res)) |
341 outf.close() | 267 outf.close() |
342 | 268 |
343 **Attribution** | 269 **Attribution** |
344 Creating re-usable tools from scripts: The Galaxy Tool Factory | 270 Creating re-usable tools from scripts: The Galaxy Tool Factory |
345 Ross Lazarus; Antony Kaspi; Mark Ziemann; The Galaxy Team | 271 Ross Lazarus; Antony Kaspi; Mark Ziemann; The Galaxy Team |
346 Bioinformatics 2012; doi: 10.1093/bioinformatics/bts573 | 272 Bioinformatics 2012; doi: 10.1093/bioinformatics/bts573 |
347 | 273 |
348 http://bioinformatics.oxfordjournals.org/cgi/reprint/bts573?ijkey=lczQh1sWrMwdYWJ&keytype=ref | 274 http://bioinformatics.oxfordjournals.org/cgi/reprint/bts573?ijkey=lczQh1sWrMwdYWJ&keytype=ref |
349 | 275 |