Mercurial > repos > fubar > weblogo3
diff weblogo3-91fa0a8ce39d/rgweblogo/rgWebLogo3.xml @ 1:a76ecde597e1 draft
Uploaded
author | fubar |
---|---|
date | Wed, 04 Dec 2013 19:58:06 -0500 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/weblogo3-91fa0a8ce39d/rgweblogo/rgWebLogo3.xml Wed Dec 04 19:58:06 2013 -0500 @@ -0,0 +1,145 @@ +<tool id="rgweblogo3" name="Sequence Logo" version="0.5"> + <stdio> + <regex match=".*" source="both" level="warning" description="chatter from weblogo:"/> + </stdio> + <requirements> + <requirement type="package" version="3.3">package_weblogo</requirement> + <requirement type="package" version="1.7.1">numpy</requirement> + <requirement type="package" version="9.07">ghostscript</requirement> + </requirements> + <description>Generator from fasta</description> + <command interpreter="python"> + rgWebLogo3.py -F $outformat -s $size -i $input -o $output -t "$logoname" -c "$colours" -U "$units" +#if $range.mode == 'part' +-l "$range.seqstart" -u "$range.seqend" +#end if + </command> + <inputs> + <page> + <param format="fasta" name="input" type="data" label="Fasta File" /> + <param name="logoname" label="Title for output Sequence Logo" type="text" size="50" value="Galaxy-Rgenetics Sequence Logo" /> + <param name="outformat" type="select" label="Output format for image (or text report)" > + <option value="png" selected="True">PNG screen quality</option> + <option value="png_print">High quality printable PNG</option> + <option value="pdf">PDF</option> + <option value="jpeg">JPG</option> + <option value="eps">EPS</option> + <option value="txt">Text (shows the detailed calculations for each position - no image)</option> + </param> + <param name="units" type="select" label="Display Units" + help="What the height of each logo element depicts - eg bits of entropy (default)"> + <option value="bits" selected="True">Entropy (bits)</option> + <option value="probability">Probability</option> + <option value="nats">Nats</option> + <option value="kT">kT</option> + <option value="kJ/mol">kJ/mol</option> + <option value="kcal/mol">kcal/mol</option> + </param> + <param name="colours" type="select" label="Colour scheme for output Sequence Logo" + help="Note that some of these only make sense for protein sequences!"> + <option value="auto" selected="True">Default automatic colour selection</option> + <option value="base pairing">Base pairing</option> + <option value="charge">Charge colours</option> + <option value="chemistry">Chemistry colours</option> + <option value="classic">Classical colours</option> + <option value="hydrophobicity">Hydrophobicity</option> + <option value="monochrome">monochrome</option> + </param> + + + <conditional name="range"> + <param name="mode" type="select" label="Include entire sequence (default) or specify a subsequence range to use"> + <option value="complete" selected="true">complete sequence</option> + <option value="part">Only use a part of the sequence</option> + </param> + <when value="complete"> + </when> + <when value="part"> + <param name="seqstart" size="5" type="integer" value="1" help="WARNING: Specifying indexes outside the sequence lengths will cause unpredictable but bad consequences!" + label="Index (eg 1=first letter) of the start of the sequence range to include in the logo"> + </param> + <param name="seqend" size="5" type="integer" value="99999" label="Index (eg 75=75th letter) of the end of the sequence range to include in the logo" > + </param> + </when> + </conditional> + <param name="size" type="select" label="Output weblogo size" > + <option value="large" selected="True">Large</option> + <option value="medium">Medium</option> + <option value="small">Small</option> + </param> + </page> + </inputs> + <outputs> + <data format="pdf" name="output" label="${logoname}.${outformat}"> + <change_format> + <when input="outformat" value="png_print" format="png" /> + <when input="outformat" value="png" format="png" /> + <when input="outformat" value="jpeg" format="jpg" /> + <when input="outformat" value="eps" format="eps" /> + <when input="outformat" value="txt" format="txt" /> + </change_format> + </data> + </outputs> + <tests> + <test> + <param name="input" value="rgClustal_testout.fasta" /> + <param name = "logoname" value="Galaxy/Rgenetics weblogo" /> + <param name = "outformat" value="jpeg" /> + <param name = "mode" value="complete" /> + <param name = "size" value="medium" /> + <param name = "colours" value="auto" /> + <param name = "units" value="bits" /> + <output name="output" file="rgWebLogo3_test.jpg" ftype="jpg" compare="sim_size" delta="10000" /> + </test> + <test> + <param name="input" value="rgClustal_testout.fasta" /> + <param name = "logoname" value="Galaxy/Rgenetics weblogo" /> + <param name = "outformat" value="png" /> + <param name = "mode" value="complete" /> + <param name = "size" value="medium" /> + <param name = "colours" value="auto" /> + <param name = "units" value="probability" /> + <output name="output" file="rgWebLogo3_test2.png" ftype="png" compare="sim_size" delta="10000" /> + </test> + </tests> + <help> + +**Note** + +This tool uses Weblogo3_ in Galaxy to generate a sequence logo. The input file must be a fasta file in your current history. + +It is recommended for (eg) viewing multiple sequence alignments output from the clustalw tool - set the output to fasta and feed +it in to this tool. + +A typical output looks like this + +.. image:: $PATH_TO_IMAGES/rgWebLogo3_test.jpg + +---- + +**Warning about input Fasta format files** + +The Weblogo3 program used by this tool will fail if your fasta sequences are not all EXACTLY the same length. The tool will provide a warning +and refuse to call the weblogo3 executable if irregular length sequences are detected. + +Fasta alignments from the companion ClustalW Galaxy tool will work but many other fasta files may cause this tool to fail - please do not file +a Galaxy bug report - this is a feature of the tool and a problem with your source data - not a tool error - please make certain all your fasta +sequences are exactly the same length! + + +**Attribution** + +Weblogo attribution and associated documentation are available at Weblogo3_ + +This Galaxy wrapper calls their software so depends on it and their license for your legal comfort. +The wrapper was written by Ross Lazarus for the rgenetics project and the source code is licensed under the LGPL_ like other rgenetics artefacts + +.. _Weblogo3: http://weblogo.threeplusone.com/manual.html + +.. _LGPL: http://www.gnu.org/copyleft/lesser.html + + </help> + +</tool> + +