Mercurial > repos > gaelcge > cellranger_atac_galaxy
diff tool_setup.md @ 0:a62bafb1aaf8 draft default tip
"planemo upload commit ff0d185541dc4b0a5839fca166f1beb459dee61c"
author | gaelcge |
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date | Tue, 02 Aug 2022 19:42:44 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_setup.md Tue Aug 02 19:42:44 2022 +0000 @@ -0,0 +1,19 @@ +# Setup for creating a Galaxy wrapper for Cellranger-ATAC with planemo + +Make sure you have a working planemo installation. To find out how to install planemo, check here: https://planemo.readthedocs.io/en/latest/installation.html + +## Generate galaxy tool xml using planemo tool_init +planemo tool_init --force \ + --id 'cellranger_atac' \ + --name 'Process 10x Genomics single-cell ATAC-seq data using CellRanger-ATAC.'\ + --example_command 'cellranger-atac count' \ + --cite_url 'https://support.10xgenomics.com/single-cell-atac/software/pipelines/latest/using/fastq-input#ATACIntro' \ + --help_from_command 'cellranger-atac' + + ## Test run with very small test data + cellranger-atac count --id=sample345 \ + --reference= \ + --fastqs=/home/florian/GenAP2/test_datasets/cellranger_ATAC/atac_pbmc_500_nextgem_fastqs/100k_sub \ + --sample=test \ + --localcores=2 \ + --localmem=6 \ No newline at end of file