Mercurial > repos > gaelcge > r_signac_galaxy
diff signac_createAssayObject.xml @ 0:6e0b320d8b6a draft default tip
"planemo upload commit dc808171975d0012e25bd7b32adc7a5a5c56a145-dirty"
author | gaelcge |
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date | Tue, 02 Aug 2022 19:11:27 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/signac_createAssayObject.xml Tue Aug 02 19:11:27 2022 +0000 @@ -0,0 +1,36 @@ +<tool id="signac_createAssayObject" name="Create Assay Object" version="0.1.0" python_template_version="3.5"> +<description> Create Assay Object and Normalize Data</description> +<macros> + <import>signac_macros.xml</import> +</macros> +<expand macro="requirements"/> +<stdio> + <exit_code range="1:" /> +</stdio> +<command><![CDATA[ + Rscript '${__tool_directory__}'/signac-createAssayObject.R --signac-object $signac_object --counts $counts --name $name --min-cells $min_cells --min-features $min_features --method $method --scale-factor $scale_factor --margin $margin --output-object-file $rds_signac_seurat_file +]]></command> +<inputs> + <param type="data" name="signac_object" label="Signac object." format="rdata" multiple="false" optional="false"/> + <param type="data" name="counts" label="Counts Matrix (Output of Gene Activity)" format="rdata" multiple="false" optional="true"/> + <param type="text" name="name" label="Assay Name." multiple="false" optional="true" value="RNA"/> + <param type="integer" name="min_cells" label="Min Cells." multiple="false" optional="false" value="0"/> + <param type="integer" name="min_features" label="Min Features." multiple="false" optional="false" value="0"/> + <param type="select" name="method" label="Method for normalization." value="LogNormalize"> + <option value="LogNormalize">LogNormalize</option> + <option value="CLR">CLR</option> + <option value="RC">RC</option> + </param> + <param type="float" name="scale_factor" value='10000' label="Sets the scale factor for cell-level normalization."/> + <param type="select" name="margin" label="If performing CLR normalization, normalize across features (1) or cells (2)." value="1"> + <option value="1">1</option> + <option value="2">2</option> + </param> +</inputs> +<outputs> + <data name="rds_signac_seurat_file" format="rdata" label="Signac Object RDS (Create Assay Object - Normalized)"/> +</outputs> +<help><![CDATA[ +This tool creates a new assay and runs the NormalizeData function. + ]]></help> +</tool>