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planemo upload for repository https://github.com/usegalaxy-au/tools-au commit 7b3bb16e4b4be0e9e2914f81cd072508ba6d5898
author | galaxy-australia |
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date | Sun, 19 Mar 2023 23:45:23 +0000 |
parents | aaa0d6a8b808 |
children | d7ce0ec78e51 |
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<tool id="cactus_export" name="Cactus: export" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@" license="MIT"> <description>whole-genome multiple sequence alignment to other formats</description> <macros> <import>macros.xml</import> </macros> <expand macro="xrefs"/> <expand macro="requirements"/> <command detect_errors="exit_code"><![CDATA[ export TMPDIR=\${_GALAXY_JOB_TMP_DIR} && ## check if the reference level was specified #if $export_select.format in ['maf_selector', 'vg_selector']: #if $export_select.ref_level == '': echo "You must provide a reference level" && exit 1 #end if #end if ln -s '$hal_file' alignment.hal ## make a MAF #if $export_select.format == 'maf_selector': && hal2maf --refGenome $export_select.ref_level alignment.hal alignment.maf #end if ## make an assemblyHub #if $export_select.format == 'ah_selector': && hal2assemblyHub.py --maxCores \${GALAXY_SLOTS:-4} --maxMemory \${GALAXY_MEMORY_MB:-8192}M ./jobStore alignment.hal assemblyhub && tar -cv assemblyhub > assemblyhub.tar #end if ## make a VG #if $export_select.format == 'vg_selector': && hal2vg alignment.hal --progress > alignment.pg #end if ]]></command> <inputs> <param name="hal_file" type="data" format="h5" label="HAL file" help="Multiple-genome alignment generated by Cactus" /> <conditional name="export_select"> <param name="format" type="select" label="Output format" help="The type of output you want to export. Run this tool once for each output format."> <option value="maf_selector" selected="true">MAF file</option> <option value="ah_selector">Assembly Hub</option> <option value="vg_selector">VG file</option> </param> <when value="maf_selector"> <param name="ref_level" type="text" value="" label="Reference genome" help="Specify one of the Input Genomes as the reference level. This must match the label used in 'Genome Label'." /> </when> <when value="ah_selector"> </when> <when value="vg_selector"> <param name="ref_level" type="text" value="" label="Reference genome" help="Specify one of the Input Genomes as the reference level. This must match the label used in 'Genome Label'." /> </when> </conditional> </inputs> <outputs> <data name="out_maf" format="maf" from_work_dir="alignment.maf" label="${tool.name} on ${on_string} (MAF file)" > <filter>export_select['format'] == 'maf_selector'</filter> </data> <data name="out_vg" format="vg" from_work_dir="alignment.pg" label="${tool.name} on ${on_string} (VG file)" > <filter>export_select['format'] == 'vg_selector'</filter> </data> <data name="out_ah" format="tar" from_work_dir="assemblyhub.tar" label="${tool.name} on ${on_string} (Assembly Hub)" > <filter>export_select['format'] == 'ah_selector'</filter> </data> </outputs> <tests> <!-- this fails because no reference level is set --> <test expect_num_outputs="1" expect_failure="true"> <conditional name="export_select"> <param name="format" value="maf_selector" /> </conditional> <param name="hal_file" ftype="h5" value="evolverMammals.hal" /> </test> <!-- within-species mode --> <test expect_num_outputs="1"> <conditional name="export_select"> <param name="ref_level" value="simMouse_chr6" /> <param name="format" value="maf_selector" /> </conditional> <param name="hal_file" ftype="h5" value="within.hal" /> <output name="out_maf" file="set_reference.maf" /> </test> <!-- between-species --> <test expect_num_outputs="1"> <conditional name="export_select"> <param name="ref_level" value="simMouse_chr6" /> <param name="format" value="maf_selector" /> </conditional> <param name="hal_file" ftype="h5" value="evolverMammals_noroot.hal" /> <output name="out_maf" file="evolverMammals_noroot.maf" /> </test> <!-- assembly hub format --> <test expect_num_outputs="1"> <conditional name="export_select"> <param name="format" value="ah_selector" /> </conditional> <param name="hal_file" ftype="h5" value="within.hal" /> <output name="out_ah"> <assert_contents> <has_archive_member path="assemblyhub/hub.txt"/> </assert_contents> </output> </test> <!-- vg format --> <test expect_num_outputs="1"> <conditional name="export_select"> <param name="format" value="vg_selector" /> <param name="ref_level" value="simMouse_chr6" /> </conditional> <param name="hal_file" ftype="h5" value="within.hal" /> <output name="out_vg"> <assert_contents> <has_size value="860452" delta="200000" /> </assert_contents> </output> </test> <!-- gfa format (gfa2): not implemented --> </tests> <help><![CDATA[ **What it does** Converts the HAL output from `Cactus whole-genome multiple sequence aligner <root?tool_id=cactus_cactus>`__ into other formats. **Input** A `HAL file <https://github.com/ComparativeGenomicsToolkit/cactus#using-the-output>`__ from `Cactus <root?tool_id=cactus_cactus>`__. **Output** Currently, you can export a pangenome in VG format, for use with `vg <https://github.com/vgteam/vg>`__, or Multiple Alignment Format (MAF). Note that these files are **reference-based**, so it will contain alignments to a single reference from your list of inputs (i.e. not a graph). You have to provide this with the ‘reference genome’ option. You can also export an Assembly Hub for use with the UCSC Genome Browser. ]]></help> <expand macro="citations"/> </tool>