Mercurial > repos > galaxy-australia > ipa_hifi
diff README.md @ 0:f78303e25f3e draft
"planemo upload for repository https://github.com/usegalaxy-au/tools-au/tree/master/tools/ipa commit 835a903a68cae7f23fd35f3adc4af28b8f508a51"
author | galaxy-australia |
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date | Wed, 08 Feb 2023 04:31:08 +0000 |
parents | |
children | 1289beb50ab0 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.md Wed Feb 08 04:31:08 2023 +0000 @@ -0,0 +1,32 @@ +*A Galaxy Wrapper of IPA HiFi Genome Assembler* + +**Description** + +This repo contains the implementation of the IPA HiFi Genome Assembler. It's currently implemented as a Snakemake workflow (workflow/ipa.snakefile) and runs the following stages: + +1. Building the SeqDB and SeedDB from the input reads. +2. Overlapping using the Pancake overlapper. +3. Phasing the overlaps using the Nighthawk phasing tool. +4. Filtering the overlaps using Falconc m4Filt. +5. Contig construction using Falcon's ovlp_to_graph and graph_to_contig tools. +6. Read tracking for read-to-contig assignment. +7. Polishing using Racon. + +For more info: https://github.com/PacificBiosciences/pbipa and https://github.com/PacificBiosciences/pbbioconda/wiki/Improved-Phased-Assembler + +*test-data* + + +The test data can be downloaded from ([bam](https://downloads.pacbcloud.com/public/dataset/2021-11-Microbial-96plex/demultiplexed-reads/m64004_210929_143746.bc2046.bam)) or select one of the bam files [here](https://downloads.pacbcloud.com/public/dataset/2021-11-Microbial-96plex/demultiplexed-reads/) or a small ccs bam (85MB) bam file [here](https://downloads.pacbcloud.com/public/dataset/ISMB_workshop/isoseq3/alz.ccs.bam) + +*planemo test* + +1. download the m64004_210929_143746.bc2046.bam file into test-data folder +2. create a softlink (test.bam) pointing to m64004_210929_143746.bc2046.bam + +3. run planemo test (i.e. planemo test --galaxy_root=/path/to/your/galaxy/folder ipa.xml) +or +3. run planemo test --update_test_data (i.e. planemo test --galaxy_root=/path/to/your/galaxy/folder ipa.xml) to update the test data +or +3. run planemoe test (i.e. planemo test --galaxy_root=/path/to/your/galaxy/folder ipa.xml) +