Mercurial > repos > galaxyp > bed_to_protein_map
diff bed_to_protein_map.xml @ 0:024ed7b0ad93 draft
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/bed_to_protein_map commit 2d39f681f77eedc840c17aebe4ddc8f66c8a7c62-dirty
author | galaxyp |
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date | Thu, 04 Jan 2018 16:29:38 -0500 |
parents | |
children | a7c58b43cbaa |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bed_to_protein_map.xml Thu Jan 04 16:29:38 2018 -0500 @@ -0,0 +1,70 @@ +<tool id="bed_to_protein_map" name="bed to protein map" version="0.1.0"> + <description>genomic location of proteins for MVP</description> + <requirements> + </requirements> + <stdio> + <exit_code range="1:" /> + </stdio> + <command><![CDATA[ + python '$__tool_directory__/bed_to_protein_map.py' '$input' '$output' + ]]></command> + <inputs> + <param name="input" type="data" format="bed" label="A BED file with 12 columns, thickStart and thickEnd define protein coding region"/> + </inputs> + <outputs> + <data name="output" format="tabular"> + <actions> + <action name="column_names" type="metadata" default="name,chrom,start,end,strand,cds_start,cds_end"/> + </actions> + </data> + </outputs> + <tests> + <test> + <param name="input" ftype="bed" value="input.bed"/> + <output name="output" file="output.tabular"/> + </test> + </tests> + <help><![CDATA[ +Convert a BED format file of the proteins from a proteomics search database into a tabular format for the Multiomics Visualization Platform (MVP). + +Example input BED dataset:: + + X 276352 291629 ENST00000430923 20 + 284187 291629 80,80,80 5 42,148,137,129,131 0,7814,12380,14295,15146 + X 304749 318819 ENST00000326153 20 - 305073 318787 80,80,80 10 448,153,149,209,159,68,131,71,138,381 0,2610,2982,6669,8016,9400,10140,10479,12164,13689 + + +Output:: + + name chrom start end strand cds_start cds_end + ENST00000430923 X 284187 284314 + 0 127 + ENST00000430923 X 288732 288869 + 127 264 + ENST00000430923 X 290647 290776 + 264 393 + ENST00000430923 X 291498 291629 + 393 524 + ENST00000326153 X 318438 318787 - 0 349 + ENST00000326153 X 316913 317051 - 349 487 + ENST00000326153 X 315228 315299 - 487 558 + ENST00000326153 X 314889 315020 - 558 689 + ENST00000326153 X 314149 314217 - 689 757 + ENST00000326153 X 312765 312924 - 757 916 + ENST00000326153 X 311418 311627 - 916 1125 + ENST00000326153 X 307731 307880 - 1125 1274 + ENST00000326153 X 307359 307512 - 1274 1427 + ENST00000326153 X 305073 305197 - 1427 1551 + + +The tabular output can be converted to a sqlite database using the Query_Tabular_ tool. + +The sqlite table should be named: feature_cds_map +The names for the columns should be: name,chrom,start,end,strand,cds_start,cds_end + +This SQL query will return the genomic location for a peptide sequence in a protein (multiply the animo acid position by 3 for the cds location):: + + SELECT distinct chrom, CASE WHEN strand = '+' THEN start + cds_offset - cds_start ELSE end - cds_offset - cds_start END as "pos" + FROM feature_cds_map + WHERE name = acc_name AND cds_offset >= cds_start AND cds_offset < cds_end + + +.. _Query_Tabular: https://toolshed.g2.bx.psu.edu/view/iuc/query_tabular/1ea4e668bf73 + + ]]></help> +</tool>