Mercurial > repos > galaxyp > gffcompare_to_bed
view gffcompare_to_bed.py @ 0:7e572e148175 draft
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/gffcompare_to_bed commit 321b217382f6be33bd77c7dbb51c8caf5fa50afe
author | galaxyp |
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date | Thu, 11 Jan 2018 11:16:51 -0500 |
parents | |
children | 9a4cfc910674 |
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#!/usr/bin/env python """ # #------------------------------------------------------------------------------ # University of Minnesota # Copyright 2017, Regents of the University of Minnesota #------------------------------------------------------------------------------ # Author: # # James E Johnson # #------------------------------------------------------------------------------ """ import argparse import sys class BedEntry(object): def __init__(self, chrom=None, chromStart=None, chromEnd=None, name=None, score=None, strand=None, thickStart=None, thickEnd=None, itemRgb=None, blockCount=None, blockSizes=None, blockStarts=None): self.chrom = chrom self.chromStart = int(chromStart) self.chromEnd = int(chromEnd) self.name = name self.score = int(score) if score is not None else 0 self.strand = '-' if str(strand).startswith('-') else '+' self.thickStart = int(thickStart) if thickStart else self.chromStart self.thickEnd = int(thickEnd) if thickEnd else self.chromEnd self.itemRgb = str(itemRgb) if itemRgb is not None else r'100,100,100' self.blockCount = int(blockCount) if isinstance(blockSizes, str) or isinstance(blockSizes, unicode): self.blockSizes = [int(x) for x in blockSizes.split(',')] elif isinstance(blockSizes, list): self.blockSizes = [int(x) for x in blockSizes] else: self.blockSizes = blockSizes if isinstance(blockStarts, str) or isinstance(blockSizes, unicode): self.blockStarts = [int(x) for x in blockStarts.split(',')] elif isinstance(blockStarts, list): self.blockStarts = [int(x) for x in blockStarts] else: self.blockStarts = blockStarts def __str__(self): return '%s\t%d\t%d\t%s\t%d\t%s\t%d\t%d\t%s\t%d\t%s\t%s' % ( self.chrom, self.chromStart, self.chromEnd, self.name, self.score, self.strand, self.thickStart, self.thickEnd, str(self.itemRgb), self.blockCount, ','.join([str(x) for x in self.blockSizes]), ','.join([str(x) for x in self.blockStarts])) def __main__(): parser = argparse.ArgumentParser( description='Retrieve Ensembl cDNAs and three frame translate') parser.add_argument( 'input', help='GFFCompare annotated GTF file, (-) for stdin') parser.add_argument( 'output', help='BED file, (-) for stdout') parser.add_argument( '-C', '--class_code', action='append', default=[], help='Restrict output to gffcompare class codes') parser.add_argument('-d', '--debug', action='store_true', help='Debug') args = parser.parse_args() # print >> sys.stderr, "args: %s" % args input_rdr = open(args.input, 'r') if args.input != '-' else sys.stdin output_wtr = open(args.output, 'w') if args.output != '-' else sys.stdout def write_bed_entry(bed): if bed.blockCount == 0: bed.blockCount = 1 output_wtr.write("%s\n" % str(bed)) class_codes = [c.strip() for codes in args.class_code for c in codes.split(',')] if args.class_code else None bed = None class_code = None for i, line in enumerate(input_rdr): if line.startswith('#'): continue fields = line.rstrip('\r\n').split('\t') if len(fields) != 9: continue (seqname, source, feature, start, end, score, strand, frame, attributes) = fields attribute = {i[0]: i[1].strip('"') for i in [j.strip().split(' ') for j in attributes.rstrip(';').split(';')]} if feature == 'transcript': if args.debug: print >> sys.stderr, "%s\t%s"\ % ('\t'.join([seqname, source, feature, start, end, score, strand, frame]), attribute) if bed is not None: write_bed_entry(bed) bed = None class_code = attribute['class_code'].strip('"')\ if 'class_code' in attribute else None if class_codes and class_code not in class_codes: continue chromStart = int(start) - 1 chromEnd = int(end) cat = '_' + class_code if class_code and class_code != '=' else '' bed = BedEntry(chrom=seqname, chromStart=chromStart, chromEnd=chromEnd, name=attribute['transcript_id'] + cat, strand=strand, blockCount=0, blockSizes=[chromEnd - chromStart], blockStarts=[0]) elif feature == 'exon' and bed is not None: chromStart = int(start) - 1 chromEnd = int(end) blockSize = chromEnd - chromStart if bed.blockCount == 0: bed.blockSizes = [] bed.blockStarts = [] bed.blockSizes.append(blockSize) bed.blockStarts.append(chromStart - bed.chromStart) bed.blockCount += 1 if bed is not None: write_bed_entry(bed) if __name__ == "__main__": __main__()