Mercurial > repos > geert-vandeweyer > coverage_report
comparison CoverageReport.xml @ 4:2799221681dd draft
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author | geert-vandeweyer |
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date | Thu, 13 Feb 2014 04:45:56 -0500 |
parents | ea32a329aced |
children | 86df3f847a72 |
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3:39c374d4cba7 | 4:2799221681dd |
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1 <tool id="CoverageReport" name="Panel Coverage Report" version="0.0.2"> | 1 <tool id="CoverageReport2" name="Panel Coverage Report" version="0.0.2"> |
2 <description></description> | 2 <description></description> |
3 | |
3 <command interpreter="perl"> | 4 <command interpreter="perl"> |
4 CoverageReport.pl | 5 CoverageReport.pl |
5 ## input files | 6 ## input files |
6 -b $input1 | 7 -b $input1 |
7 -t $input2 | 8 -t $input2 |
25 #else: | 26 #else: |
26 -n "Unspecified" | 27 -n "Unspecified" |
27 #end if | 28 #end if |
28 </command> | 29 </command> |
29 <requirements> | 30 <requirements> |
30 <requirement type="package">BEDTools</requirement> | 31 <requirement type="package" version="2.11.0">R</requirement> |
31 <requirement type="package">samtools</requirement> | 32 <requirement type="package" version="2.18.2">bedtools</requirement> |
33 <requirement type="package" version="0.1.18">samtools</requirement> | |
32 <requirement type="binary">pdflatex</requirement> | 34 <requirement type="binary">pdflatex</requirement> |
33 </requirements> | 35 </requirements> |
34 <inputs> | 36 <inputs> |
35 <param name="input1" type="data" format="bam" label="BAM file" help="BAM file of mapped reads" /> | 37 <param name="input1" type="data" format="bam" label="BAM file" help="BAM file of mapped reads" /> |
36 <param name="input2" type="data" format="bed" label="Target Regions BED" help="BED file containing regions of interest. See below for format" /> | 38 <param name="input2" type="data" format="bed" label="Target Regions BED" help="BED file containing regions of interest. See below for format" /> |
78 The BED file containing targets of interest has very specific format requirements. You **must** use the following format:: | 80 The BED file containing targets of interest has very specific format requirements. You **must** use the following format:: |
79 | 81 |
80 Column 1: Chromosome : Use the same syntax as the references used by Galaxy. Check your sam-headers for the correct format. ('chr1' vs '1') | 82 Column 1: Chromosome : Use the same syntax as the references used by Galaxy. Check your sam-headers for the correct format. ('chr1' vs '1') |
81 Column 2: Start Position | 83 Column 2: Start Position |
82 Column 3: End Position | 84 Column 3: End Position |
83 Column 4: Target Name. Use : "GENE-NAME|Exon_number" : This is split on the 'Pipe' after 'GeneName' for correct grouping. | 85 Column 4: Target Name. Use : "GENE-NAME<space>Exon_number" : This is split on the space after 'GeneName' for correct grouping. |
84 Column 5: Score : ignored, use '0' | 86 Column 5: Score : ignored, use '0' |
85 Column 6: Strand: ignored,'+' or '-' | 87 Column 6: Strand: ignored,'+' or '-' |
86 | 88 |
87 .. class:: infomark | 89 .. class:: infomark |
88 | 90 |
109 | 111 |
110 **Targets.Position.Coverage** : Original BED file + following columns:: | 112 **Targets.Position.Coverage** : Original BED file + following columns:: |
111 - Position in target region | 113 - Position in target region |
112 - Coverage at position | 114 - Coverage at position |
113 | 115 |
114 ------ | |
115 | |
116 **Requirements** | |
117 | |
118 - BEDTools (from toolshed) | |
119 - Samtools (from toolshed) | |
120 - pdflatex : binary must be in path, to create the pdf report. | |
121 | |
122 </help> | 116 </help> |
123 </tool> | 117 </tool> |
124 | 118 |
125 | 119 |