Mercurial > repos > genouest > feelnc2asko
view feelnc2asko.pl @ 0:e323c49b8bcc draft
planemo upload for repository https://github.com/genouest/galaxy-tools/tree/master/tools/feelnc2asko commit 92849224db1963d090fbb25d410cc659a5449241
author | genouest |
---|---|
date | Thu, 12 Apr 2018 06:05:23 -0400 |
parents | |
children | af75f883cab4 |
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use strict; use warnings; use Getopt::Long; use Bio::Tools::GFF; my ($anngff, $lncgff, $newgff); GetOptions("ann=s" => \$anngff, "lnc=s" => \$lncgff, "new=s" => \$newgff); my $gffout = Bio::Tools::GFF->new(-fh=> \*STDOUT, -gff_version => 3); #1. the standard annotation my $gffin = Bio::Tools::GFF->new(-file => $anngff, -gff_version => 3); while (my $feature = $gffin->next_feature()) { if ($feature-> primary_tag eq 'mRNA') { my ($gene)=$feature->get_tag_values("gene"); $feature->remove_tag("Parent"); $feature->add_tag_value("Parent", $gene); $feature->add_tag_value("feelnc_type", "standard"); $gffout->write_feature($feature); } if ($feature-> primary_tag eq 'gene') { my ($name)=$feature->get_tag_values("Name"); $feature->remove_tag("ID"); $feature->add_tag_value("ID", $name); $feature->add_tag_value("feelnc_type", "standard"); $gffout->write_feature($feature); } } $gffin->close(); my %genes=(); my %transcripts=(); #2. The lncRNA gtf my $fncgtf = Bio::Tools::GFF->new( -file => $lncgff, -gff_version => '2' ); while (my $feat = $fncgtf->next_feature()) { next if ($feat->primary_tag() ne 'exon'); my $mrna= ($feat->get_tag_values('transcript_id'))[0]; my $gene= ($feat->get_tag_values('gene_id'))[0]; # print STDERR "str: ", $feat->strand(), "\n"; if (exists($genes{$gene})) { if ($genes{$gene}->start() > $feat->start()) { $genes{$gene}->start($feat->start()); } if ($genes{$gene}->end() < $feat->end()) { $genes{$gene}->end($feat->end()); } } else { my $geneft = Bio::SeqFeature::Generic->new( -start => $feat->start(), -end => $feat->end(), -strand => $feat->strand(), -primary_tag => 'gene', -source_tag => $feat->source_tag(), -seq_id => $feat->seq_id()); # $geneft->add_tag_value("feelnc_type", "lncRNA"); $genes{$gene}=$geneft; } if (exists($transcripts{$mrna})) { if ($transcripts{$mrna}->start() > $feat->start()) { $transcripts{$mrna}->start($feat->start()); } if ($transcripts{$mrna}->end() < $feat->end()) { $transcripts{$mrna}->end($feat->end()); } } else { my $tr = Bio::SeqFeature::Generic->new( -start => $feat->start(), -end => $feat->end(), -strand => $feat->strand(), -primary_tag => 'mRNA', -source_tag => $feat->source_tag(), -seq_id => $feat->seq_id()); $tr->add_tag_value("ID", $mrna); $tr->add_tag_value("Parent",$gene); $tr->add_tag_value("feelnc_type", "lncRNA"); $transcripts{$mrna}=$tr; } } #3. The new mRNA gtf my $nmgtf = Bio::Tools::GFF->new( -file => $newgff, -gff_version => '2' ); while (my $feat = $nmgtf->next_feature()) { next if ($feat->primary_tag() ne 'exon'); my $mrna= ($feat->get_tag_values('transcript_id'))[0]; my $gene= ($feat->get_tag_values('gene_id'))[0]; # print STDERR "str: ", $feat->strand() , "\n"; if (exists($genes{$gene})) { if ($genes{$gene}->start() > $feat->start()) { $genes{$gene}->start($feat->start()); } if ($genes{$gene}->end() < $feat->end()) { $genes{$gene}->end($feat->end()); } } else { my $geneft = Bio::SeqFeature::Generic->new( -start => $feat->start(), -end => $feat->end(), -strand => $feat->strand(), -primary_tag => 'gene', -source_tag => $feat->source_tag(), -seq_id => $feat->seq_id()); $geneft->add_tag_value("ID", $gene); # $geneft->add_tag_value("feelnc_type", "new"); $genes{$gene}=$geneft; } if (exists($transcripts{$mrna})) { if ($transcripts{$mrna}->start() > $feat->start()) { $transcripts{$mrna}->start($feat->start()); } if ($transcripts{$mrna}->end() < $feat->end()) { $transcripts{$mrna}->end($feat->end()); } } else { my $tr = Bio::SeqFeature::Generic->new( -start => $feat->start(), -end => $feat->end(), -strand => $feat->strand(), -primary_tag => 'mRNA', -source_tag => $feat->source_tag(), -seq_id => $feat->seq_id()); $tr->add_tag_value("ID", $mrna); $tr->add_tag_value("Parent",$gene); $tr->add_tag_value("feelnc_type", "new"); $transcripts{$mrna}=$tr; } } foreach my $mrna (keys %transcripts) { my ($parent)=$transcripts{$mrna}->get_tag_values("Parent"); $gffout->write_feature($genes{$parent}); $gffout->write_feature($transcripts{$mrna}); }