Mercurial > repos > george-weingart > lefse
diff home/ubuntu/lefse_to_export/plot_cladogram.py @ 1:db64b6287cd6 draft
Modified datatypes
author | george-weingart |
---|---|
date | Wed, 20 Aug 2014 16:56:51 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/home/ubuntu/lefse_to_export/plot_cladogram.py Wed Aug 20 16:56:51 2014 -0400 @@ -0,0 +1,342 @@ +#!/usr/bin/env python + +import os,sys,matplotlib,argparse,string +matplotlib.use('Agg') +from pylab import * +from lefse import * +import numpy as np + +colors = ['r','g','b','m','c',[1.0,0.5,0.0],[0.0,1.0,0.0],[0.33,0.125,0.0],[0.75,0.75,0.75],'k'] +dark_colors = [[0.4,0.0,0.0],[0.0,0.2,0.0],[0.0,0.0,0.4],'m','c',[1.0,0.5,0.0],[0.0,1.0,0.0],[0.33,0.125,0.0],[0.75,0.75,0.75],'k'] + +class CladeNode: + def __init__(self, name, abundance, viz = True): + self.id = name + self.name = name.split(".") + self.last_name = self.name[-1] + self.abundance = abundance + self.pos = (-1.0,-1.0) + self.children = {} + self.isleaf = True + self.color = 'y' + self.next_leaf = -1 + self.prev_leaf = -1 + self.viz = viz + def __repr__(self): + return self.last_name + def add_child(self,node): + self.isleaf = False + self.children[node.__repr__()] = node + def get_children(self): + ck = sorted(self.children.keys()) + return [self.children[k] for k in ck] + def get_color(self): + return self.color + def set_color(self,c): + self.color = c + def set_pos(self,pos): + self.pos = pos + +def read_params(args): + parser = argparse.ArgumentParser(description='Cladoplot') + parser.add_argument('input_file', metavar='INPUT_FILE', type=str, help="tab delimited input file") + parser.add_argument('output_file', metavar='OUTPUT_FILE', type=str, help="the file for the output image") + parser.add_argument('--clade_sep',dest="clade_sep", type=float, default=1.5) + parser.add_argument('--max_lev',dest="max_lev", type=int, default=-1) + parser.add_argument('--max_point_size',dest="max_point_size", type=float, default=6.0) + parser.add_argument('--min_point_size',dest="min_point_size", type=float, default=1) + parser.add_argument('--point_edge_width',dest="markeredgewidth", type=float, default=.25) + parser.add_argument('--siblings_connector_width',dest="siblings_connector_width", type=float, default=2) + parser.add_argument('--parents_connector_width',dest="parents_connector_width", type=float, default=0.75) + parser.add_argument('--radial_start_lev',dest="radial_start_lev", type=int, default=1) + parser.add_argument('--labeled_start_lev',dest="labeled_start_lev", type=int, default=2) + parser.add_argument('--labeled_stop_lev',dest="labeled_stop_lev", type=int, default=5) + parser.add_argument('--abrv_start_lev',dest="abrv_start_lev", type=int, default=3) + parser.add_argument('--abrv_stop_lev',dest="abrv_stop_lev", type=int, default=5) + parser.add_argument('--expand_void_lev',dest="expand_void_lev", type=int, default=1) + parser.add_argument('--class_legend_vis',dest="class_legend_vis", type=int, default=1) + parser.add_argument('--colored_connector',dest="colored_connectors", type=int, default=1) + parser.add_argument('--alpha',dest="alpha", type=float, default=0.2) + parser.add_argument('--title',dest="title", type=str, default="Cladogram") + parser.add_argument('--sub_clade',dest="sub_clade", type=str, default="") + parser.add_argument('--title_font_size',dest="title_font_size", type=str, default="14") + parser.add_argument('--right_space_prop',dest="r_prop", type=float, default=0.1) + parser.add_argument('--left_space_prop',dest="l_prop", type=float, default=0.1) + parser.add_argument('--label_font_size',dest="label_font_size", type=str, default="6") + parser.add_argument('--background_color',dest="back_color", type=str, choices=["k","w"], default="w", help="set the color of the background") + parser.add_argument('--colored_labels',dest="col_lab", type=int, choices=[0,1], default=1, help="draw the label with class color (1) or in black (0)") + parser.add_argument('--class_legend_font_size',dest="class_legend_font_size", type=str, default="10") + parser.add_argument('--dpi',dest="dpi", type=int, default=72) + parser.add_argument('--format', dest="format", choices=["png","svg","pdf"], default="svg", type=str, help="the format for the output file") + parser.add_argument('--all_feats', dest="all_feats", type=str, default="") + args = parser.parse_args() + return vars(args) + +def cmp_names(la,lb): + if len(la) != len(lb): return False + for p in [(a,b) for i,a in enumerate(la) for j,b in enumerate(lb) if i == j]: + if p[0] != p[1]: return False + return True + +def build_tree(father,all_nodes,l,depth,viz): + cc = [n for n in all_nodes if len(n.name) > len(father.name) and cmp_names(father.name,n.name[:len(father.name)])] + children = [n for n in cc if len(n.name) == len(father.name)+1] + if len(children) == 0 and l < depth -1: # !!! + nc = CladeNode(father.id+"."+father.id.split(".")[-1],1.0,viz) + father.add_child(nc) + children.append(nc) + for child in children: + build_tree(child,cc,l+1,depth,viz) + father.add_child(child) + +def get_all_nodes(father): + ret = [father] + children = father.get_children() + for c in children: + ret += get_all_nodes(c) + return ret + +def read_data(input_file,params): + with open(input_file, 'r') as inp: + if params['sub_clade'] == "": rows = [line.strip().split()[:-1] for line in inp.readlines() if params['max_lev'] < 1 or line.split()[0].count(".") < params['max_lev']] + else: rows = [line.split(params['sub_clade']+".")[1].strip().split()[:-1] for line in inp.readlines() if ( params['max_lev'] < 1 or line.split()[0].count(".") < params['max_lev'] ) and line.startswith(params['sub_clade']+".")] + all_names = [lin[0] for lin in rows] + to_add = [] + + abundances = [float(v) for v in zip(*rows)[1] if v >= 0.0] + tree = {} + tree['classes'] = list(set([v[2] for v in rows if len(v)>2])) + tree['classes'].sort() + all_nodes = [CladeNode("root."+row[0],float(row[1])) for row in rows] + + depth = max([len(n.name) for n in all_nodes]) + + n2 = ["_".join(nn.name) for nn in all_nodes] + for i,nn in enumerate(all_nodes): + n = nn + while "_".join(n.name[:-1]) not in n2 and len(n.name) > 1: + n = CladeNode(".".join(n.name[:-1]),n.abundance) + all_nodes.append(n) + n2.append("_".join(n.name)) + + cls2 = [] + if params['all_feats'] != "": + cls2 = sorted(params['all_feats'].split(":")) + for i,v in enumerate(rows): + if len(v)>2: + if len(cls2) > 0: all_nodes[i].set_color(colors[cls2.index(v[2])%len(colors)]) + else: + if v[2].count('rgbcol') > 0: + ccc = [float(tt) for tt in v[2].split('_')[1:]] + all_nodes[i].set_color(ccc) + else: all_nodes[i].set_color(colors[sorted(tree['classes']).index(v[2])%len(colors)]) + root = CladeNode("root",-1.0) + root.set_pos((0.0,0.0)) + + build_tree(root,all_nodes,0,depth,params['expand_void_lev']==1) + + all_nodes = get_all_nodes(root) + + tree['root'] = root + tree['max_abs'] = max(abundances) + tree['min_abs'] = min(abundances) + levs = [] + for i in range(depth): + depthi = [n for n in all_nodes if len(n.name) == i+1] + levs.append(len(depthi)) + tree['nlev'] = levs + return tree + +def add_all_pos(father,n,distn,seps,tsep,mlev,last_leaf=-1,nc=1): + children = father.get_children() + leaves = True if children[0].isleaf else False + for i,child in enumerate(children): + if leaves: + n += 1.0 + men = 0.5 if len(children) == 1 else 0.0 + child.set_pos((n*distn-men*float(distn)+tsep,(len(father.name))/float(mlev-1))) + if last_leaf != -1: + child.prev_leaf = last_leaf + last_leaf.next_leaf = child + last_leaf = child + else: + ln = n + ltsep = tsep + n,tsep,last_leaf = add_all_pos(child,n,distn,seps,tsep,mlev,last_leaf,len(children)) + nn = (ln + n)*0.5*distn + ssep = (ltsep + tsep)*0.5 + if n-ln == 1: + ssep = ltsep + child.set_pos((nn+ssep,(len(father.name))/float(mlev-1))) + tsep += seps[len(father.name)-1] + return n,tsep,last_leaf + +def plot_points(father,params,pt_scale,ax): + children = father.get_children() + children.sort(key = lambda a: -int(a.get_color() == 'y')*a.abundance) + x,r = father.pos[0], father.pos[1] + for i,child in enumerate(children): + xc,rc = plot_points(child,params,pt_scale,ax) + if not father.viz: return x,r + ps = pt_scale[0]+father.abundance/pt_scale[1]+pt_scale[0] + col = father.get_color() + pw = params['markeredgewidth'] if col == 'y' else params['markeredgewidth']*3.0 + if x==0 and r==0: ax.plot(x,r, 'o',markersize=ps,color=col,markeredgewidth=0.01,markeredgecolor=params['fore_color']) + else: ax.plot(x,r, 'o',markersize=ps,color=col,markeredgewidth=pw,markeredgecolor=params['fore_color']) + return x,r + +def plot_lines(father,params,depth,ax,xf): + children = father.get_children() + x,r = father.pos[0], father.pos[1] + for i,child in enumerate(children): + xc,rc = plot_lines(child,params,depth,ax,x) + if i == 0: x_first, r_first = xc, rc + if len(father.name) >= depth-params['radial_start_lev']: + col = params['fore_color'] + lw=params['parents_connector_width'] + if not child.viz: continue + if father.get_color() != 'y' and father.get_color() == child.get_color() and params['colored_connectors']: + col = child.get_color() + lw *=2.5 + if col != params['fore_color']: + ax.plot([x,xc],[r,rc],"-",color=params['fore_color'],lw=lw*1.5) + ax.plot([x,xc],[r,rc],"-",color=col,lw=lw) + + if not father.viz or (len(children) == 1 and not children[0].viz): return x,r + if len(father.name) < depth-params['radial_start_lev']: + col = params['fore_color'] + lw=params['parents_connector_width'] + if father.get_color() != 'y': + f =True + for child in children: + if child.get_color() != father.get_color() or not params['colored_connectors']: + f = False + break + if f: + col = father.get_color() + lw *= 2.5 + if not (x==0 and r==0): + xx = xc if len(children) > 0 else x + if len(children) == 0: rc = r + xt = x if len(children)>1 else xx + if col != params['fore_color']: + ax.plot([x,xt],[r,rc],"-",color=params['fore_color'],lw=lw*1.5) + ax.plot([x,xt],[r,rc],"-",color=col,lw=lw) + if len(children) > 0 and 1 < len(father.name) < depth-params['radial_start_lev']: + xs = arange(x_first,xc,0.01) + ys = [rc for t in xs] + ax.plot(xs,ys,"-",color=col,lw=params['siblings_connector_width'],markeredgecolor=params['fore_color']) + return x,r + +def uniqueid(): + for l in string.lowercase: yield l + for l in string.lowercase: + for i in range(10): + yield l+str(i) + i = 0 + while True: + yield str(i) + i += 1 + +def plot_names(father,params,depth,ax,u_i,seps): + children = father.get_children() + l = len(father.name) + if len(children)==0: + if father.prev_leaf == -1 or father.next_leaf == -1: + fr_0, fr_1 = father.pos[0], father.pos[0] + else: fr_0, fr_1 = (father.pos[0]+father.prev_leaf.pos[0])*0.5, (father.pos[0]+father.next_leaf.pos[0])*0.5 + for i,child in enumerate(children): + fr,to = plot_names(child,params,depth,ax,u_i,seps) + if i == 0: fr_0 = fr + fr_1 = to + if father.get_color() != 'y' and params['labeled_start_lev'] < l <= params['labeled_stop_lev']+1: + col = father.get_color() + dd = params['labeled_stop_lev'] - params['labeled_start_lev'] + 1 + de = depth - 1 + dim = 1.0/float(de) + perc_ext = 0.65 if dim > 0.1 else 1.0 + clto = (de-l+1)*dim+dim*(dd+1-(l-dd-1))*perc_ext + clto = (de-l+1)*dim+dim*(dd-(l-params['labeled_start_lev'])+1)*perc_ext + des = float(180.0*(fr_0+fr_1)/np.pi)*0.5-90 + lab = "" + txt = father.last_name + if params['abrv_start_lev'] < l <= params['abrv_stop_lev'] + 1: + ide = u_i.next() + lab = str(ide)+": "+father.last_name + txt = str(ide) +# ax.bar(fr_0, clto, width = fr_1-fr_0, bottom = float(l-1)/float(depth-1), alpha = params['alpha'], color=col, edgecolor=col) + ax.bar(fr_0, clto, width = fr_1-fr_0, bottom = float(l-1)/float(de), alpha = params['alpha'], color=col, edgecolor=col) + ax.bar(0.0, 0.0, width = 0.0, bottom = 0.0, alpha = 1.0, color=col, edgecolor=params['fore_color'], label=lab) + if l <= params['abrv_stop_lev'] + 1: + if not params['col_lab']: col = params['fore_color'] + else: + if col not in colors: col = params['fore_color'] + else: col = dark_colors[colors.index(col)%len(dark_colors)] + ax.text((fr_0+fr_1)*0.5, clto+float(l-1)/float(de)-dim*perc_ext/2.0, txt, size = params['label_font_size'], rotation=des, ha ="center", va="center", color=col) + return fr_0, fr_1 + +def draw_tree(out_file,tree,params): + plt_size = 7 + nlev = tree['nlev'] + pt_scale = (params['min_point_size'],max(1.0,((tree['max_abs']-tree['min_abs']))/(params['max_point_size']-params['min_point_size']))) + depth = len(nlev) + sep = (2.0*np.pi)/float(nlev[-1]) + seps = [params['clade_sep']*sep/float(depth-i+1) for i in range(1,len(tree['nlev'])+1)] + totseps = sum([s*nlev[i] for i,s in enumerate(seps[:-1])]) + clade_sep_err = True if totseps > np.pi else False + while totseps > np.pi: + params['clade_sep'] *= 0.75 + seps = [params['clade_sep']*sep/(float(depth-i+1)*0.25) for i in range(1,len(tree['nlev'])+1)] + totseps = sum([s*nlev[i] for i,s in enumerate(seps[:-1])]) + if clade_sep_err: print 'clade_sep parameter too large, lowered to',params['clade_sep'] + + fig = plt.figure(edgecolor=params['back_color'],facecolor=params['back_color']) + ax = fig.add_subplot(111, polar=True, frame_on=False, axis_bgcolor=params['back_color'] ) + plt.subplots_adjust(right=1.0-params['r_prop'],left=params['l_prop']) + ax.grid(False) + xticks([]) + yticks([]) + + ds = (2.0*np.pi-totseps)/float(nlev[-1]) + + add_all_pos(tree['root'],0.0,ds,seps,0.0,depth) + + plot_lines(tree['root'],params,depth,ax,0) + plot_points(tree['root'],params,pt_scale,ax) + plot_names(tree['root'],params,depth,ax,uniqueid(),seps) + + r = np.arange(0, 3.0, 0.01) + theta = 2*np.pi*r + + def get_col_attr(x): + return hasattr(x, 'set_color') and not hasattr(x, 'set_facecolor') + + h, l = ax.get_legend_handles_labels() + if len(l) > 0: + leg = ax.legend(bbox_to_anchor=(1.05, 1), frameon=False, loc=2, borderaxespad=0.,prop={'size':params['label_font_size']}) + if leg != None: + gca().add_artist(leg) + for o in leg.findobj(get_col_attr): + o.set_color(params['fore_color']) + + cll = sorted(tree['classes']) if params['all_feats'] == "" else sorted(params['all_feats'].split(":")) + nll = [ax.bar(0.0, 0.0, width = 0.0, bottom = 0.0, color=colors[i%len(colors)], label=c) for i,c in enumerate(cll) if c in tree['classes']] + cl = [c for c in cll if c in tree['classes']] + + ax.set_title(params['title'],size=params['title_font_size'],color=params['fore_color']) + + if params['class_legend_vis']: + l2 = legend(nll, cl, loc=2, prop={'size':params['class_legend_font_size']}, frameon=False) + if l2 != None: + for o in l2.findobj(get_col_attr): + o.set_color(params['fore_color']) + + plt.savefig(out_file,format=params['format'],facecolor=params['back_color'],edgecolor=params['fore_color'],dpi=params['dpi']) + plt.close() + +if __name__ == '__main__': + params = read_params(sys.argv) + params['fore_color'] = 'w' if params['back_color'] == 'k' else 'k' + clad_tree = read_data(params['input_file'],params) + draw_tree(params['output_file'],clad_tree,params) +