Mercurial > repos > gga > apollo_feat_from_gff3
diff export.py @ 0:5aa3bc8d0253 draft
planemo upload for repository https://github.com/galaxy-genome-annotation/galaxy-tools/tree/master/tools/apollo commit f745b23c84a615bf434d717c8c0e553a012f0268
author | gga |
---|---|
date | Mon, 11 Sep 2017 05:45:37 -0400 |
parents | |
children | 7c4a3e737e08 |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/export.py Mon Sep 11 05:45:37 2017 -0400 @@ -0,0 +1,88 @@ +#!/usr/bin/env python +from __future__ import print_function + +import argparse +import json +import sys + +from BCBio import GFF + +from Bio import SeqIO + +from future import standard_library + +from webapollo import CnOrGuess, GuessCn, WAAuth, WebApolloInstance + +standard_library.install_aliases() +try: + import StringIO as io +except ImportError: + import io + + +def export(org_cn, seqs): + org_data = wa.organisms.findOrganismByCn(org_cn) + + data = io.StringIO() + + kwargs = dict( + exportType='GFF3', + seqType='genomic', + exportGff3Fasta=True, + output="text", + exportFormat="text", + organism=org_cn, + ) + + if len(seqs) > 0: + data.write(wa.io.write( + exportAllSequences=False, + sequences=seqs, + **kwargs + ).encode('utf-8')) + else: + data.write(wa.io.write( + exportAllSequences=True, + sequences=[], + **kwargs + ).encode('utf-8')) + + # Seek back to start + data.seek(0) + + records = list(GFF.parse(data)) + if len(records) == 0: + print("Could not find any sequences or annotations for this organism + reference sequence") + sys.exit(2) + else: + for record in records: + record.annotations = {} + record.features = sorted(record.features, key=lambda x: x.location.start) + if args.gff: + GFF.write([record], args.gff) + record.description = "" + if args.fasta: + SeqIO.write([record], args.fasta, 'fasta') + + return org_data + + +if __name__ == '__main__': + parser = argparse.ArgumentParser(description='Sample script to add an attribute to a feature via web services') + WAAuth(parser) + CnOrGuess(parser) + parser.add_argument('--gff', type=argparse.FileType('w')) + parser.add_argument('--fasta', type=argparse.FileType('w')) + parser.add_argument('--json', type=argparse.FileType('w')) + + args = parser.parse_args() + + wa = WebApolloInstance(args.apollo, args.username, args.password) + + org_cn_list, seqs = GuessCn(args, wa) + + org_data = [] + for org_cn in org_cn_list: + indiv_org_data = export(org_cn, seqs) + org_data.append(indiv_org_data) + args.json.write(json.dumps(org_data, indent=2))