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2
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1 LOCUS Exported 6850 bp ds-DNA circular SYN 13-NOV-2018
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2 DEFINITION synthetic circular DNA
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3 ACCESSION .
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4 VERSION .
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5 KEYWORDS .
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6 SOURCE synthetic DNA construct
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7 ORGANISM synthetic DNA construct
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8 REFERENCE 1 (bases 1 to 6850)
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9 AUTHORS .
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10 TITLE Direct Submission
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11 JOURNAL Exported Nov 13, 2018 from SnapGene 4.2.6
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12 http://www.snapgene.com
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13 COMMENT LOCUS dna 6850 bp
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14 FEATURES Location/Qualifiers
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15 source 1..6850
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16 /organism="synthetic DNA construct"
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17 /mol_type="other DNA"
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18 misc_feature 1..819
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19 /label=p15A origin of replication region
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20 /note="p15A origin of replication region"
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21 misc_feature 1032..3625
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22 /label=Staphylococcus aureus plasmid pC194
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23 /note="Staphylococcus aureus plasmid pC194 region
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24 (NC_002013.1)"
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25 misc_feature 1126..1324
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26 /label=Repeat sequence 1 of 2
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27 /note="Repeat sequence 1 of 2"
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28 CDS complement(1562..2212)
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29 /label=Chloramphenicol Resistance
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30 CDS complement(2346..2744)
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31 /label=Potential Copy number associated protein
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32 /note="Potential Copy number associated protein"
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33 misc_feature 2463..2517
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34 /label=pC194 replication origin
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35 /note="pC194 replication origin (detailed in PMC401278)"
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36 misc_feature 2670..3605
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37 /label=Replication initiation protein Rep RC
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38 misc_feature 3626..3824
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39 /label=Repeat sequence 2 of 2
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40 /note="Repeat sequence 2 of 2"
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41 misc_feature 3825..5278
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42 /label=Xylose Inducible Promoter Region
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43 /note="Xylose Inducible Promoter Region, Xyl promoter, Xyl
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44 repressor protein, Xyl terminator sequence and pTX5
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45 promoter facing MCS:
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46 http://parts.igem.org/Part:BBa_K1323014"
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47 CDS 4000..5151
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48 /label=XylR repressor protein
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49 misc_feature 5191..5241
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50 /label=Bacteriophage T5 PN25 promoter
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51 /note="Bacteriophage T5 PN25 promoter (Deuschle et al,
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52 PMC1167251)"
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53 misc_feature 5206..5211
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54 /label=-30 region
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55 /note="-30 region"
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56 misc_feature 5229..5234
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57 /label=-10 region
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58 /note="-10 region"
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59 misc_feature 5241..5279
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60 /label=Xyl operator containing palindromic
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61 /note="interfering with transcription initiation"
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62 misc_feature 5279..5317
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63 /label=MCS
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64 /note="MCS"
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65 terminator 5393..5550
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66 /label=rrnB term
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67 terminator 5516..5559
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68 /label=rrnB_T1 term
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69 terminator 5691..5718
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70 /label=rrnB_T2 term
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71 misc_feature complement(5860..6720)
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72 /label=Ampicillin Resistance
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73 /note="gene of the plasmid pLEX5BA"
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74 promoter complement(6762..6790)
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75 /label=amp prom
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76 ORIGIN
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77 1 ggcggccgca ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
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78 61 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
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79 121 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
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80 181 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
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81 241 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
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82 301 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
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83 361 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
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84 421 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
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85 481 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
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86 541 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
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87 601 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
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88 661 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
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89 721 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
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90 781 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatg cggccgcttc tttcctgcgt
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91 841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
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92 901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
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93 961 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataggaaga tccctcgacc
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94 1021 tgcaggcatg caagcttctg taggttttta ggcataaaac tatatgattt acccctaaat
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95 1081 ctttaaaatg ccccttaaaa ttcaaaataa aggcatttaa aatttaaata tttcttgtga
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96 1141 taaagtttgt taaaaaggag tggttttatg actgttatgt ggttatcgat tataggtatg
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97 1201 tggttttgta ttggaatggc attttttgct atcaaggtta ttaaaaataa aaattagacc
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98 1261 acgcatttat gccgagaaaa tttattgtgc gttgagaaga acccttaact aaacttgcag
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99 1321 acgaatgtcg gcatagcgtg agctattaag ccgaccattc gacaagtttt gggattgtta
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100 1381 agggttccga ggctcaacgt caataaagca attggaataa agaagcgaaa aaggagaagt
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101 1441 cggttcagaa aaagaaggat atggatctgg agctgtaata taaaaacctt cttcaactaa
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102 1501 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat
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103 1561 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc
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104 1621 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg
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105 1681 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat
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106 1741 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag
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107 1801 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat
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108 1861 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag
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109 1921 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac
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110 1981 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg
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111 2041 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata
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112 2101 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat
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113 2161 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg
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114 2221 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
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115 2281 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
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116 2341 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac
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117 2401 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
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118 2461 ttttctttct tatcttgata ataagggtaa ctattgccgg cgaggctagt tacccttaag
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119 2521 ttattggtat gactggtttt aagcgcaaaa aaagttgctt tttcgtacct attaatgtat
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120 2581 cgttttaaat gaatagtaaa aaacatacat agaaagggga aaaagcaact ttttttattg
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121 2641 tcatagtttg tgaaaactaa gttgttttta tgtgttataa catggaaaag tatactgaga
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122 2701 aaaaacaaag aaatcaagta tttcagaaat ttattaaacg tcatattgga gagaatcaaa
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123 2761 tggatttagt tgaagattgc aatacatttc tgtcttttgt agctgataaa actttagaaa
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124 2821 aacagaaatt atataaagct aattcttgta aaaatcgatt ttgtcctgtc tgtgcttgga
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125 2881 gaaaagctag aaaagatgca ttgggtttat ctttgatgat gcaatatatt aagcagcaag
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126 2941 agaaaaagga gtttatcttt ttaactttga ctacacctaa tgtaatgagt gatgaattag
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127 3001 aaaatgaaat aaaacgttat aataattctt ttagaaaact tataaagaga aaaaaagtag
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128 3061 gtagtgttat aaagggatat gttcgtaagt tagagattac atataataaa aaaagagatg
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129 3121 attataatcc tcattttcat gtgttaattg cagtaaataa atcgtatttc acagataaaa
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130 3181 gatattatat tagccaacaa gaatggttag atttatggcg tgatgtaacg ggcatttcag
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131 3241 aaataacaca agttcaagtt caaaaaataa gacaaaataa taataaagaa ttatatgaaa
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132 3301 tggctaagta ttctggtaaa gatagtgatt atttaataaa tcaaaaagtc tttgatgcat
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133 3361 tttataaatc acttaaaggt aaacaggtat tagtttattc aggattattt aaagaggcta
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134 3421 aaaagaaatt aaaaaatggg gatttagatt acttaaaaga aattgatcca accgaatata
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135 3481 tctatcaaat tttttatatt tggaaacaaa aagagtattt agctagtgaa ctttatgact
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136 3541 taacagaaca agaaaaaaga gaaattaatc acaaaatgat agacgaaatc gaggaagaac
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137 3601 aataacaaaa tataagtgct aacagtcgtc tgcaagttta gttaagggtt cttctcaacg
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138 3661 cacaataaat tttctcggca taaatgcgtg gtctaatttt tatttttaat aaccttgata
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139 3721 gcaaaaaatg ccattccaat acaaaaccac atacctataa tcgataacca cataacagtc
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140 3781 ataaaaccac tcctttttaa caaactttat cacaagaaat attttggcat tctacgacta
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141 3841 taacttaaat ttatattttt tactttataa tatataattg attatagaat aatgttgctc
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142 3901 atatcgtttg ccaacatcta gtactcaaat tacactatgt tacacttggt aatattaacc
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143 3961 gaacttcccc tgtccaaatt agataagagg taataataaa tggaaaataa ttttatagta
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144 4021 aatgaaaatg agaagcgtgt attaaaacaa attttcaata acagcaatat ttcacgaaca
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145 4081 caaatatcga agaatttaga acttaataaa gctactattt ctaacattct gaacaactta
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146 4141 aaacacaaga gtttagttaa tgaagtagga gaaggtaata gtactaaaag tggtggacga
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147 4201 aagcctattt tactcgaaat taaccaaaaa tatggctact atatttctat ggatttaaca
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148 4261 tatgattccg ttgaattaat gtacaactac tttgatgcta ctatattaaa gcaagattcc
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149 4321 tacgaattaa atgataaaaa tgtaagcagt atattacaaa ttttaaaatc taatataaac
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150 4381 gtctcagaaa aatatgatac gttatatggg ttacttggta tatctatatc catacacggt
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151 4441 atcgttgacg atgagcaaaa cataatcaat cttccttttc ataaaaatga gaaacgcaca
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152 4501 tttaccgatg aattaaagtc attcacaaat gttcctgtcg ttatagaaaa tgaagcaaat
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153 4561 ttatcagcgc tatatgaaaa aagtttatat attaattcaa acataaataa tttgattact
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154 4621 ttaagtattc acaagggtat aggcgctggc atcctaataa ataaaaaact ttatcgtggc
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155 4681 tcaaatggag aggctggaga gataggtaag acattggttt tggaatctat aaataacaat
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156 4741 gacaacaaat attataaaat cgaagatata tgctcccaag acgctttaat acagaaaata
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157 4801 aataataggt tgggcgtcac attgacgttt acagaactaa tccaatatta caacgaagga
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158 4861 aattcaattg ttgctcatga aattaaacaa tttattaata aaatgacagt tctgattcat
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159 4921 aatttgaata cacaatttaa cccagacgct atttatatta actgtccttt aattaatgaa
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160 4981 ttaccaaata ttttaaatga aattaaagag caattctcct gtttttctca aggcagtcca
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161 5041 gttcaattac atttaactac taatgtaaaa caagctactt tattgggtgg cactttagca
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162 5101 ataatgcaaa aaacattaaa tataaataac attcaaatga atattaaata attacagcag
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163 5161 tctgagttat aaaatagata tctcggaccg tcataaaaaa tttatttgct ttcaggaaaa
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164 5221 tttttctgta taatagattc aagttagttt gtttattaaa ttaaccaact aaaatgtaga
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165 5281 attcgagctc ggtacccggg gatcctctag agtcgacctg cagccaagct tgggcttttc
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166 5341 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
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167 5401 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
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168 5461 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
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169 5521 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
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170 5581 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
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171 5641 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
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172 5701 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
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173 5761 caaatatgta tccgctcatc cccatcctat cgatgataag ctgtcaaaca tgagaattaa
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174 5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
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175 5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
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176 5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
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177 6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
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178 6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
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179 6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
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180 6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
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181 6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
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182 6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
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183 6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
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184 6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
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185 6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgctcttc
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186 6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
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187 6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
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188 6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
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189 6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
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190 6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
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191 6841 agtgccacct
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192 //
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