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comparison CodonSwitchTool/pEPSA5_annotated.gb @ 2:aad5e435e4dc draft default tip

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author gianmarco_piccinno
date Tue, 21 May 2019 05:24:56 -0400
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1:1c31d6d25429 2:aad5e435e4dc
1 LOCUS Exported 6850 bp ds-DNA circular SYN 13-NOV-2018
2 DEFINITION synthetic circular DNA
3 ACCESSION .
4 VERSION .
5 KEYWORDS .
6 SOURCE synthetic DNA construct
7 ORGANISM synthetic DNA construct
8 REFERENCE 1 (bases 1 to 6850)
9 AUTHORS .
10 TITLE Direct Submission
11 JOURNAL Exported Nov 13, 2018 from SnapGene 4.2.6
12 http://www.snapgene.com
13 COMMENT LOCUS dna 6850 bp
14 FEATURES Location/Qualifiers
15 source 1..6850
16 /organism="synthetic DNA construct"
17 /mol_type="other DNA"
18 misc_feature 1..819
19 /label=p15A origin of replication region
20 /note="p15A origin of replication region"
21 misc_feature 1032..3625
22 /label=Staphylococcus aureus plasmid pC194
23 /note="Staphylococcus aureus plasmid pC194 region
24 (NC_002013.1)"
25 misc_feature 1126..1324
26 /label=Repeat sequence 1 of 2
27 /note="Repeat sequence 1 of 2"
28 CDS complement(1562..2212)
29 /label=Chloramphenicol Resistance
30 CDS complement(2346..2744)
31 /label=Potential Copy number associated protein
32 /note="Potential Copy number associated protein"
33 misc_feature 2463..2517
34 /label=pC194 replication origin
35 /note="pC194 replication origin (detailed in PMC401278)"
36 misc_feature 2670..3605
37 /label=Replication initiation protein Rep RC
38 misc_feature 3626..3824
39 /label=Repeat sequence 2 of 2
40 /note="Repeat sequence 2 of 2"
41 misc_feature 3825..5278
42 /label=Xylose Inducible Promoter Region
43 /note="Xylose Inducible Promoter Region, Xyl promoter, Xyl
44 repressor protein, Xyl terminator sequence and pTX5
45 promoter facing MCS:
46 http://parts.igem.org/Part:BBa_K1323014"
47 CDS 4000..5151
48 /label=XylR repressor protein
49 misc_feature 5191..5241
50 /label=Bacteriophage T5 PN25 promoter
51 /note="Bacteriophage T5 PN25 promoter (Deuschle et al,
52 PMC1167251)"
53 misc_feature 5206..5211
54 /label=-30 region
55 /note="-30 region"
56 misc_feature 5229..5234
57 /label=-10 region
58 /note="-10 region"
59 misc_feature 5241..5279
60 /label=Xyl operator containing palindromic
61 /note="interfering with transcription initiation"
62 misc_feature 5279..5317
63 /label=MCS
64 /note="MCS"
65 terminator 5393..5550
66 /label=rrnB term
67 terminator 5516..5559
68 /label=rrnB_T1 term
69 terminator 5691..5718
70 /label=rrnB_T2 term
71 misc_feature complement(5860..6720)
72 /label=Ampicillin Resistance
73 /note="gene of the plasmid pLEX5BA"
74 promoter complement(6762..6790)
75 /label=amp prom
76 ORIGIN
77 1 ggcggccgca ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
78 61 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
79 121 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
80 181 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
81 241 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
82 301 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
83 361 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
84 421 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
85 481 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
86 541 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
87 601 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
88 661 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
89 721 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
90 781 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatg cggccgcttc tttcctgcgt
91 841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
92 901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
93 961 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataggaaga tccctcgacc
94 1021 tgcaggcatg caagcttctg taggttttta ggcataaaac tatatgattt acccctaaat
95 1081 ctttaaaatg ccccttaaaa ttcaaaataa aggcatttaa aatttaaata tttcttgtga
96 1141 taaagtttgt taaaaaggag tggttttatg actgttatgt ggttatcgat tataggtatg
97 1201 tggttttgta ttggaatggc attttttgct atcaaggtta ttaaaaataa aaattagacc
98 1261 acgcatttat gccgagaaaa tttattgtgc gttgagaaga acccttaact aaacttgcag
99 1321 acgaatgtcg gcatagcgtg agctattaag ccgaccattc gacaagtttt gggattgtta
100 1381 agggttccga ggctcaacgt caataaagca attggaataa agaagcgaaa aaggagaagt
101 1441 cggttcagaa aaagaaggat atggatctgg agctgtaata taaaaacctt cttcaactaa
102 1501 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat
103 1561 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc
104 1621 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg
105 1681 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat
106 1741 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag
107 1801 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat
108 1861 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag
109 1921 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac
110 1981 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg
111 2041 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata
112 2101 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat
113 2161 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg
114 2221 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
115 2281 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
116 2341 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac
117 2401 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
118 2461 ttttctttct tatcttgata ataagggtaa ctattgccgg cgaggctagt tacccttaag
119 2521 ttattggtat gactggtttt aagcgcaaaa aaagttgctt tttcgtacct attaatgtat
120 2581 cgttttaaat gaatagtaaa aaacatacat agaaagggga aaaagcaact ttttttattg
121 2641 tcatagtttg tgaaaactaa gttgttttta tgtgttataa catggaaaag tatactgaga
122 2701 aaaaacaaag aaatcaagta tttcagaaat ttattaaacg tcatattgga gagaatcaaa
123 2761 tggatttagt tgaagattgc aatacatttc tgtcttttgt agctgataaa actttagaaa
124 2821 aacagaaatt atataaagct aattcttgta aaaatcgatt ttgtcctgtc tgtgcttgga
125 2881 gaaaagctag aaaagatgca ttgggtttat ctttgatgat gcaatatatt aagcagcaag
126 2941 agaaaaagga gtttatcttt ttaactttga ctacacctaa tgtaatgagt gatgaattag
127 3001 aaaatgaaat aaaacgttat aataattctt ttagaaaact tataaagaga aaaaaagtag
128 3061 gtagtgttat aaagggatat gttcgtaagt tagagattac atataataaa aaaagagatg
129 3121 attataatcc tcattttcat gtgttaattg cagtaaataa atcgtatttc acagataaaa
130 3181 gatattatat tagccaacaa gaatggttag atttatggcg tgatgtaacg ggcatttcag
131 3241 aaataacaca agttcaagtt caaaaaataa gacaaaataa taataaagaa ttatatgaaa
132 3301 tggctaagta ttctggtaaa gatagtgatt atttaataaa tcaaaaagtc tttgatgcat
133 3361 tttataaatc acttaaaggt aaacaggtat tagtttattc aggattattt aaagaggcta
134 3421 aaaagaaatt aaaaaatggg gatttagatt acttaaaaga aattgatcca accgaatata
135 3481 tctatcaaat tttttatatt tggaaacaaa aagagtattt agctagtgaa ctttatgact
136 3541 taacagaaca agaaaaaaga gaaattaatc acaaaatgat agacgaaatc gaggaagaac
137 3601 aataacaaaa tataagtgct aacagtcgtc tgcaagttta gttaagggtt cttctcaacg
138 3661 cacaataaat tttctcggca taaatgcgtg gtctaatttt tatttttaat aaccttgata
139 3721 gcaaaaaatg ccattccaat acaaaaccac atacctataa tcgataacca cataacagtc
140 3781 ataaaaccac tcctttttaa caaactttat cacaagaaat attttggcat tctacgacta
141 3841 taacttaaat ttatattttt tactttataa tatataattg attatagaat aatgttgctc
142 3901 atatcgtttg ccaacatcta gtactcaaat tacactatgt tacacttggt aatattaacc
143 3961 gaacttcccc tgtccaaatt agataagagg taataataaa tggaaaataa ttttatagta
144 4021 aatgaaaatg agaagcgtgt attaaaacaa attttcaata acagcaatat ttcacgaaca
145 4081 caaatatcga agaatttaga acttaataaa gctactattt ctaacattct gaacaactta
146 4141 aaacacaaga gtttagttaa tgaagtagga gaaggtaata gtactaaaag tggtggacga
147 4201 aagcctattt tactcgaaat taaccaaaaa tatggctact atatttctat ggatttaaca
148 4261 tatgattccg ttgaattaat gtacaactac tttgatgcta ctatattaaa gcaagattcc
149 4321 tacgaattaa atgataaaaa tgtaagcagt atattacaaa ttttaaaatc taatataaac
150 4381 gtctcagaaa aatatgatac gttatatggg ttacttggta tatctatatc catacacggt
151 4441 atcgttgacg atgagcaaaa cataatcaat cttccttttc ataaaaatga gaaacgcaca
152 4501 tttaccgatg aattaaagtc attcacaaat gttcctgtcg ttatagaaaa tgaagcaaat
153 4561 ttatcagcgc tatatgaaaa aagtttatat attaattcaa acataaataa tttgattact
154 4621 ttaagtattc acaagggtat aggcgctggc atcctaataa ataaaaaact ttatcgtggc
155 4681 tcaaatggag aggctggaga gataggtaag acattggttt tggaatctat aaataacaat
156 4741 gacaacaaat attataaaat cgaagatata tgctcccaag acgctttaat acagaaaata
157 4801 aataataggt tgggcgtcac attgacgttt acagaactaa tccaatatta caacgaagga
158 4861 aattcaattg ttgctcatga aattaaacaa tttattaata aaatgacagt tctgattcat
159 4921 aatttgaata cacaatttaa cccagacgct atttatatta actgtccttt aattaatgaa
160 4981 ttaccaaata ttttaaatga aattaaagag caattctcct gtttttctca aggcagtcca
161 5041 gttcaattac atttaactac taatgtaaaa caagctactt tattgggtgg cactttagca
162 5101 ataatgcaaa aaacattaaa tataaataac attcaaatga atattaaata attacagcag
163 5161 tctgagttat aaaatagata tctcggaccg tcataaaaaa tttatttgct ttcaggaaaa
164 5221 tttttctgta taatagattc aagttagttt gtttattaaa ttaaccaact aaaatgtaga
165 5281 attcgagctc ggtacccggg gatcctctag agtcgacctg cagccaagct tgggcttttc
166 5341 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
167 5401 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
168 5461 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
169 5521 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
170 5581 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
171 5641 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
172 5701 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
173 5761 caaatatgta tccgctcatc cccatcctat cgatgataag ctgtcaaaca tgagaattaa
174 5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
175 5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
176 5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
177 6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
178 6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
179 6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
180 6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
181 6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
182 6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
183 6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
184 6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
185 6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgctcttc
186 6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
187 6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
188 6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
189 6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
190 6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
191 6841 agtgccacct
192 //