LOCUS       Exported                6850 bp ds-DNA     circular SYN 13-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 13, 2018 from SnapGene 4.2.6
            http://www.snapgene.com
COMMENT     LOCUS dna 6850 bp
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..819
                     /label=p15A origin of replication region
                     /note="p15A origin of replication region"
     misc_feature    1032..3625
                     /label=Staphylococcus aureus plasmid pC194
                     /note="Staphylococcus aureus plasmid pC194 region
                     (NC_002013.1)"
     misc_feature    1126..1324
                     /label=Repeat sequence 1 of 2
                     /note="Repeat  sequence 1 of 2"
     CDS             complement(1562..2212)
                     /label=Chloramphenicol Resistance
     CDS             complement(2346..2744)
                     /label=Potential Copy number associated protein
                     /note="Potential Copy number associated protein"
     misc_feature    2463..2517
                     /label=pC194 replication origin
                     /note="pC194 replication origin (detailed in PMC401278)"
     misc_feature    2670..3605
                     /label=Replication initiation protein Rep RC
     misc_feature    3626..3824
                     /label=Repeat sequence 2 of 2
                     /note="Repeat sequence 2 of 2"
     misc_feature    3825..5278
                     /label=Xylose Inducible Promoter Region
                     /note="Xylose Inducible Promoter Region, Xyl promoter, Xyl
                     repressor protein, Xyl terminator sequence and pTX5
                     promoter facing MCS:
                     http://parts.igem.org/Part:BBa_K1323014"
     CDS             4000..5151
                     /label=XylR repressor protein
     misc_feature    5191..5241
                     /label=Bacteriophage T5 PN25 promoter
                     /note="Bacteriophage T5 PN25 promoter (Deuschle et al,
                     PMC1167251)"
     misc_feature    5206..5211
                     /label=-30 region
                     /note="-30 region"
     misc_feature    5229..5234
                     /label=-10 region
                     /note="-10 region"
     misc_feature    5241..5279
                     /label=Xyl operator containing palindromic
                     /note="interfering with transcription initiation"
     misc_feature    5279..5317
                     /label=MCS
                     /note="MCS"
     terminator      5393..5550
                     /label=rrnB term
     terminator      5516..5559
                     /label=rrnB_T1 term
     terminator      5691..5718
                     /label=rrnB_T2 term
     misc_feature    complement(5860..6720)
                     /label=Ampicillin Resistance
                     /note="gene of the plasmid pLEX5BA"
     promoter        complement(6762..6790)
                     /label=amp prom
ORIGIN
        1 ggcggccgca ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
       61 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
      121 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
      181 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
      241 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
      301 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      361 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
      421 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
      481 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
      601 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
      661 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
      721 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
      781 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatg cggccgcttc tttcctgcgt
      841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
      961 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataggaaga tccctcgacc
     1021 tgcaggcatg caagcttctg taggttttta ggcataaaac tatatgattt acccctaaat
     1081 ctttaaaatg ccccttaaaa ttcaaaataa aggcatttaa aatttaaata tttcttgtga
     1141 taaagtttgt taaaaaggag tggttttatg actgttatgt ggttatcgat tataggtatg
     1201 tggttttgta ttggaatggc attttttgct atcaaggtta ttaaaaataa aaattagacc
     1261 acgcatttat gccgagaaaa tttattgtgc gttgagaaga acccttaact aaacttgcag
     1321 acgaatgtcg gcatagcgtg agctattaag ccgaccattc gacaagtttt gggattgtta
     1381 agggttccga ggctcaacgt caataaagca attggaataa agaagcgaaa aaggagaagt
     1441 cggttcagaa aaagaaggat atggatctgg agctgtaata taaaaacctt cttcaactaa
     1501 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat
     1561 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc
     1621 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg
     1681 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat
     1741 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag
     1801 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat
     1861 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag
     1921 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac
     1981 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg
     2041 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata
     2101 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat
     2161 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg
     2221 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
     2281 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
     2341 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac
     2401 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     2461 ttttctttct tatcttgata ataagggtaa ctattgccgg cgaggctagt tacccttaag
     2521 ttattggtat gactggtttt aagcgcaaaa aaagttgctt tttcgtacct attaatgtat
     2581 cgttttaaat gaatagtaaa aaacatacat agaaagggga aaaagcaact ttttttattg
     2641 tcatagtttg tgaaaactaa gttgttttta tgtgttataa catggaaaag tatactgaga
     2701 aaaaacaaag aaatcaagta tttcagaaat ttattaaacg tcatattgga gagaatcaaa
     2761 tggatttagt tgaagattgc aatacatttc tgtcttttgt agctgataaa actttagaaa
     2821 aacagaaatt atataaagct aattcttgta aaaatcgatt ttgtcctgtc tgtgcttgga
     2881 gaaaagctag aaaagatgca ttgggtttat ctttgatgat gcaatatatt aagcagcaag
     2941 agaaaaagga gtttatcttt ttaactttga ctacacctaa tgtaatgagt gatgaattag
     3001 aaaatgaaat aaaacgttat aataattctt ttagaaaact tataaagaga aaaaaagtag
     3061 gtagtgttat aaagggatat gttcgtaagt tagagattac atataataaa aaaagagatg
     3121 attataatcc tcattttcat gtgttaattg cagtaaataa atcgtatttc acagataaaa
     3181 gatattatat tagccaacaa gaatggttag atttatggcg tgatgtaacg ggcatttcag
     3241 aaataacaca agttcaagtt caaaaaataa gacaaaataa taataaagaa ttatatgaaa
     3301 tggctaagta ttctggtaaa gatagtgatt atttaataaa tcaaaaagtc tttgatgcat
     3361 tttataaatc acttaaaggt aaacaggtat tagtttattc aggattattt aaagaggcta
     3421 aaaagaaatt aaaaaatggg gatttagatt acttaaaaga aattgatcca accgaatata
     3481 tctatcaaat tttttatatt tggaaacaaa aagagtattt agctagtgaa ctttatgact
     3541 taacagaaca agaaaaaaga gaaattaatc acaaaatgat agacgaaatc gaggaagaac
     3601 aataacaaaa tataagtgct aacagtcgtc tgcaagttta gttaagggtt cttctcaacg
     3661 cacaataaat tttctcggca taaatgcgtg gtctaatttt tatttttaat aaccttgata
     3721 gcaaaaaatg ccattccaat acaaaaccac atacctataa tcgataacca cataacagtc
     3781 ataaaaccac tcctttttaa caaactttat cacaagaaat attttggcat tctacgacta
     3841 taacttaaat ttatattttt tactttataa tatataattg attatagaat aatgttgctc
     3901 atatcgtttg ccaacatcta gtactcaaat tacactatgt tacacttggt aatattaacc
     3961 gaacttcccc tgtccaaatt agataagagg taataataaa tggaaaataa ttttatagta
     4021 aatgaaaatg agaagcgtgt attaaaacaa attttcaata acagcaatat ttcacgaaca
     4081 caaatatcga agaatttaga acttaataaa gctactattt ctaacattct gaacaactta
     4141 aaacacaaga gtttagttaa tgaagtagga gaaggtaata gtactaaaag tggtggacga
     4201 aagcctattt tactcgaaat taaccaaaaa tatggctact atatttctat ggatttaaca
     4261 tatgattccg ttgaattaat gtacaactac tttgatgcta ctatattaaa gcaagattcc
     4321 tacgaattaa atgataaaaa tgtaagcagt atattacaaa ttttaaaatc taatataaac
     4381 gtctcagaaa aatatgatac gttatatggg ttacttggta tatctatatc catacacggt
     4441 atcgttgacg atgagcaaaa cataatcaat cttccttttc ataaaaatga gaaacgcaca
     4501 tttaccgatg aattaaagtc attcacaaat gttcctgtcg ttatagaaaa tgaagcaaat
     4561 ttatcagcgc tatatgaaaa aagtttatat attaattcaa acataaataa tttgattact
     4621 ttaagtattc acaagggtat aggcgctggc atcctaataa ataaaaaact ttatcgtggc
     4681 tcaaatggag aggctggaga gataggtaag acattggttt tggaatctat aaataacaat
     4741 gacaacaaat attataaaat cgaagatata tgctcccaag acgctttaat acagaaaata
     4801 aataataggt tgggcgtcac attgacgttt acagaactaa tccaatatta caacgaagga
     4861 aattcaattg ttgctcatga aattaaacaa tttattaata aaatgacagt tctgattcat
     4921 aatttgaata cacaatttaa cccagacgct atttatatta actgtccttt aattaatgaa
     4981 ttaccaaata ttttaaatga aattaaagag caattctcct gtttttctca aggcagtcca
     5041 gttcaattac atttaactac taatgtaaaa caagctactt tattgggtgg cactttagca
     5101 ataatgcaaa aaacattaaa tataaataac attcaaatga atattaaata attacagcag
     5161 tctgagttat aaaatagata tctcggaccg tcataaaaaa tttatttgct ttcaggaaaa
     5221 tttttctgta taatagattc aagttagttt gtttattaaa ttaaccaact aaaatgtaga
     5281 attcgagctc ggtacccggg gatcctctag agtcgacctg cagccaagct tgggcttttc
     5341 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     5401 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     5461 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     5521 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     5581 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     5641 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     5701 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     5761 caaatatgta tccgctcatc cccatcctat cgatgataag ctgtcaaaca tgagaattaa
     5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgctcttc
     6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6841 agtgccacct
//
