Mercurial > repos > glogobyte > armdb
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author | glogobyte |
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date | Mon, 16 Oct 2023 14:27:43 +0000 |
parents | f5b6cad56b62 |
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<tool id="ArmDB" name="ArmDB: Generation of Pre-miRNA arm reference DB (5p and 3p arms)" version="0.1.1"> <requirements> <requirement type="package" version="2.28.0">bedtools</requirement> <requirement type="package" version="3.7.4">python</requirement> </requirements> <command> #if $mir_input.analysis == "1": #set path=$mir_input.genome1.fields.dbkey python $__tool_directory__/armdb_mirbase.py -pos $positions -tool_dir $__tool_directory__ -gff3 "$path" -gen $mir_input.genome1.fields.value #end if #if $mir_input.analysis == "2": #set path=$mir_input.genome2.fields.value python $__tool_directory__/armdb_mirgene.py -pos $positions -tool_dir $__tool_directory__ -sym "$path" #end if </command> <inputs> <conditional name="mir_input"> <param name="analysis" type="select" label="Choose reference Mir database" help="Choose which database prefer to be used."> <option value="1" selected="true">MirBase</option> <option value="2">MirGene</option> </param> <when value="1"> <param name="genome1" type="select" label="Choose organism" help="If your genome coordinates of interest is not listed, contact the Galaxy team"> <options from_data_table="mirbase" /> </param> </when> <when value="2"> <param name="genome2" type="select" label="Choose organism" help="If your genome coordinates of interest is not listed, contact the" > <options from_data_table="mirgene" /> </param> </when> </conditional> <param name="positions" type="integer" min="0" max="8" value="1" label="Custom Arm length" help="Choose how many nucleotides you want to extend the 5p and 3p mature sequences to create the refernce arms" /> </inputs> <outputs> <data name="shifted_gff1" format="txt" label="Custom Bed file" from_work_dir="$__tool_directory__/shifted_mirnas.bed"> <filter>mir_input['analysis']== "1" </filter> </data> <data name="normal_gff1" format="fasta" label="Official Bed file" from_work_dir="$__tool_directory__/original_mirnas.bed" > <filter>mir_input['analysis']== "1" </filter> </data> <data name="custom reference genome1" format="fasta" label="Custom reference arms" from_work_dir="$__tool_directory__/new_ref.fa" > <filter>mir_input['analysis']== "1" </filter> </data> <data name="shifted_gff" format="txt" label="Mature and star miRNA sequences" from_work_dir="$__tool_directory__/shifted_mirnas.bed"> <filter>mir_input['analysis']== "2" </filter> </data> <data name="normal_gff" format="fasta" label="Primary transcripts with flanks" from_work_dir="$__tool_directory__/original_mirnas.bed" > <filter>mir_input['analysis']== "2" </filter> </data> <data name="custom reference genome" format="fasta" label="Custom reference arms" from_work_dir="$__tool_directory__/new_ref.fa" > <filter>mir_input['analysis']== "2" </filter> </data> </outputs> <help> </help> </tool>