Mercurial > repos > glogobyte > mirviz
diff viz.py @ 5:4d59ac081282 draft
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author | glogobyte |
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date | Wed, 13 Oct 2021 11:19:17 +0000 |
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children | 0a4d6c483ff0 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/viz.py Wed Oct 13 11:19:17 2021 +0000 @@ -0,0 +1,96 @@ +from viz_graphs import * +from viz_functions import * +import argparse +import time +from multiprocessing import Process, Lock, Manager + +################################################################################################################################################################################################################################################################## +starttime = time.time() + +parser = argparse.ArgumentParser() +parser.add_argument("-in", "--input", help="choose type of analysis", action="store") +parser.add_argument("-p_value", "--pval", help="choose type of analysis", action="store") +parser.add_argument("-fc", "--log2fc", help="choose type of analysis", action="store") +parser.add_argument("-top", "--top_mirnas", help="choose type of analysis", action="store") +parser.add_argument("-tool_dir", "--tool_directory", help="tool directory path", action="store") +parser.add_argument("-statistic", "--stat", help="tool directory path", action="store") +parser.add_argument("-diff_tool", "--tool", help="tool directory path", action="store") + +args = parser.parse_args() + +l=Lock() +number = int(args.top_mirnas) +log2fc = float(args.log2fc) +pval = float(args.pval) +iso_star_flag=0 +non_star_flag=0 + +if args.tool=="2": + + raw_EdgeR = read(args.input,0) + EdgeR = [x.rstrip("\n").split("\t") for x in raw_EdgeR] + del EdgeR[0] + for x in EdgeR: + if "/" in x[0]: + x[0]=x[0].split("/")[0]+"★" + + if args.stat=="1": + non_templated = [[x[0],x[1],x[4]] for x in EdgeR if "nt" in x[0] and x[1]!="NA" and x[4]!="NA"] + matures = [[x[0],x[1],x[4]] for x in EdgeR if 'chr' in x[0].split("_")[-1] and "nt" not in x[0] and x[1]!="NA" and x[4]!="NA"] + isoforms = [[x[0],x[1],x[4]] for x in EdgeR if 'chr' not in x[0].split("_")[-1] and "nt" not in x[0] and x[1]!="NA" and x[4]!="NA"] + else: + non_templated = [[x[0],x[1],x[5]] for x in EdgeR if "nt" in x[0] and x[1]!="NA" and x[5]!="NA"] + matures = [[x[0],x[1],x[5]] for x in EdgeR if 'chr' in x[0].split("_")[-1] and "nt" not in x[0] and x[1]!="NA" and x[5]!="NA"] + isoforms = [[x[0],x[1],x[5]] for x in EdgeR if 'chr' not in x[0].split("_")[-1] and "nt" not in x[0] and x[1]!="NA" and x[5]!="NA"] + +if args.tool=="1": + + raw_Deseq = read(args.input,0) + Deseq = [x.rstrip("\n").split("\t") for x in raw_Deseq] + for x in Deseq: + if "/" in x[0]: + x[0]=x[0].split("/")[0]+"★" + + if args.stat=="1": + non_templated = [[x[0],x[2],x[5]] for x in Deseq if "nt" in x[0] and x[2]!="NA" and x[5]!="NA"] + matures = [[x[0],x[2],x[5]] for x in Deseq if 'chr' in x[0].split("_")[-1] and "nt" not in x[0] and x[2]!="NA" and x[5]!="NA"] + isoforms = [[x[0],x[2],x[5]] for x in Deseq if 'chr' not in x[0].split("_")[-1] and "nt" not in x[0] and x[2]!="NA" and x[5]!="NA"] + elif args.stat=="2": + non_templated = [[x[0],x[2],x[6]] for x in Deseq if "nt" in x[0] and x[2]!="NA" and x[6]!="NA"] + matures = [[x[0],x[2],x[6]] for x in Deseq if 'chr' in x[0].split("_")[-1] and "nt" not in x[0] and x[2]!="NA" and x[6]!="NA"] + isoforms = [[x[0],x[2],x[6]] for x in Deseq if 'chr' not in x[0].split("_")[-1] and "nt" not in x[0] and x[2]!="NA" and x[6]!="NA"] + else: + non_templated = [[x[0],x[2],x[1]] for x in Deseq if "nt" in x[0] and x[2]!="NA" and x[1]!="NA"] + matures = [[x[0],x[2],x[1]] for x in Deseq if 'chr' in x[0].split("_")[-1] and "nt" not in x[0] and x[2]!="NA" and x[1]!="NA"] + isoforms = [[x[0],x[2],x[1]] for x in Deseq if 'chr' not in x[0].split("_")[-1] and "nt" not in x[0] and x[2]!="NA" and x[1]!="NA"] + + +diff_matures,diff_isoforms,diff_non_templated,names,non_temp,mat_iso = preproccess(non_templated,matures,isoforms,log2fc,pval,args.stat) +for x in mat_iso[:number]: + if "★" in x[0]: + iso_star_flag=1 + break +for x in non_temp[:number]: + if "★" in x[0]: + non_star_flag=1 + break + + +if non_templated!=[]: + analysis="2" + p=[Process(target=top_diff,args=(non_temp,number,"nt",l))] + p.extend([Process(target=top_diff,args=(mat_iso,number,"t",l))]) + p.extend([Process(target=top_scatter_non,args=(diff_matures,diff_isoforms,diff_non_templated,names,number))]) + +else: + analysis="1" + p=[Process(target=top_diff,args=(mat_iso,number,"t",l))] + p.extend([Process(target=top_scatter_tem,args=(diff_matures,diff_isoforms,names,number))]) + +[x.start() for x in p] +[x.join() for x in p] + +pdf_after_DE(analysis,args.top_mirnas,args.tool_directory,iso_star_flag,non_star_flag) + +print('That took {} seconds'.format(time.time() - starttime)) +