Mercurial > repos > goeckslab > scimap_phenotyping

annotate scimap_spatial.py @ 2:ce22e846c5e4 draft

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planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit 9fb5578191db8a559191e45156cfb95350f01aea
author goeckslab
date Mon, 10 Jun 2024 18:44:25 +0000
parents dcfcad35e847
children 3a441d347a46
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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diff changeset
1 import argparse
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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2 import json
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goeckslab
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3 import warnings
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goeckslab
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5 import scimap as sm
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goeckslab
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6 from anndata import read_h5ad
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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9 def main(inputs, anndata, output):
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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10 """
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goeckslab
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11 Parameter
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12 ---------
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13 inputs : str
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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14 File path to galaxy tool parameter.
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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15 anndata : str
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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16 File path to anndata containing phenotyping info.
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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17 output : str
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18 File path to output.
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goeckslab
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19 """
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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20 warnings.simplefilter('ignore')
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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22 with open(inputs, 'r') as param_handler:
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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23 params = json.load(param_handler)
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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25 adata = read_h5ad(anndata)
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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27 tool = params['analyses']['selected_tool']
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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28 tool_func = getattr(sm.tl, tool)
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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30 options = params['analyses']['options']
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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31 if tool == 'cluster':
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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32 options['method'] = params['analyses']['method']
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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33 subset_genes = options.pop('subset_genes')
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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34 if subset_genes:
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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35 options['subset_genes'] = \
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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36 [x.strip() for x in subset_genes.split(',')]
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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37 sub_cluster_group = options.pop('sub_cluster_group')
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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38 if sub_cluster_group:
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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39 options['sub_cluster_group'] = \
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
goeckslab
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40 [x.strip() for x in sub_cluster_group.split(',')]
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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42 for k, v in options.items():
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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43 if v == '':
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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44 options[k] = None
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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46 tool_func(adata, **options)
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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47
1
dcfcad35e847 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit d20d6f5d08b43de71182459f8a6452c564a5becc
goeckslab
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48 if tool == 'spatial_lda':
dcfcad35e847 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit d20d6f5d08b43de71182459f8a6452c564a5becc
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49 adata.uns.pop('spatial_lda_model')
dcfcad35e847 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit d20d6f5d08b43de71182459f8a6452c564a5becc
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50
0
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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51 adata.write(output)
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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53
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54 if __name__ == '__main__':
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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55 aparser = argparse.ArgumentParser()
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56 aparser.add_argument("-i", "--inputs", dest="inputs", required=True)
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57 aparser.add_argument("-e", "--output", dest="output", required=True)
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58 aparser.add_argument("-a", "--anndata", dest="anndata", required=True)
7ed4b55b11f7 planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit b19cb55dfb751cccc857b95a432890299bfeebb5
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60 args = aparser.parse_args()
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61
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62 main(args.inputs, args.anndata, args.output)