Mercurial > repos > greg > affy2vcf
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author | greg |
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date | Fri, 30 Nov 2018 08:23:05 -0500 |
parents | 02025c366fca |
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<tool id="affr2vcf" name="Convert Affymetrix" version="@WRAPPER_VERSION@"> <description>genotype calls and intensities to VCF</description> <macros> <import>macros.xml</import> </macros> <requirements> <requirement type="package" version="1.9">bcftools-gtc2vcf-plugin</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ @PREPARE_ENV@ #set text_outputs_dir = 'text_outputs' mkdir $text_outputs_dir && bcftools +\$BCFTOOLS_PLUGINS/affy2vcf.so #if str($reference_genome_source_cond.reference_genome_source) == "history": --fasta-ref '$reference_genome_source_cond.history_item' #else: --fasta-ref '$reference_genome_source_cond.locally_cached_item.fields.path' #end if --annot '$annot' --snp-posteriors '$snp_posteriors' --summary '$summary' --report '$report' --calls '$calls' --confidences '$confidences' #if str($output_gender_estimate_cond.output_gender_estimate) == "yes" --sex '$output_gender_estimate' #end if #if str($append_version) == "no": --no-version #end if --output '$output' --output-type $output_type @THREADS@ ]]></command> <inputs> <param name="annot" type="data" format="csv" label="Probeset annotation file" /> <param name="summary" type="data" format="txt" label="Apt-probeset genotype summary file" /> <param name="snp_posteriors" type="data" format="txt" label="Apt-probeset genotype snp-posteriors file" /> <param name="report" type="data" format="txt" label="Apt-probeset genotype report file" /> <param name="confidences" type="data" format="txt" label="Apt-probeset genotype confidences file" /> <param name="calls" type="data" format="txt" label="Apt-probeset genotype calls file" /> <conditional name="reference_genome_source_cond"> <param name="reference_genome_source" type="select" label="Choose the source for the reference genome"> <option value="history" selected="true">Use a reference genome from my history</option> <option value="cached">Use a locally cached genome index</option> </param> <when value="history"> <param name="history_item" type="data" format="fasta" label="Select reference genome" /> </when> <when value="cached"> <param name="locally_cached_item" type="select" format="fasta" label="Fasta reference sequence"> <options from_data_table="all_fasta"> <column name="name" index="2"/> <column name="value" index="0"/> <column name="path" index="3"/> <filter type="sort_by" column="1"/> <validator type="no_options" message="No cached Fasta genome references are available for the build associated with the selected probeset annotation file." /> </options> </param> </when> </conditional> <conditional name="output_gender_estimate_cond"> <param name="output_gender_estimate" type="select" force_select="true" label="Output apt-probeset-genotype gender estimate?"> <option value="no" selected="true">No</option> <option value="yes">Yes</option> </param> <when value="no"/> <when value="yes"/> </conditional> <param name="append_version" type="select" force_select="true" label="Append version and command line to the header?"> <option value="no" selected="true">No</option> <option value="yes">Yes</option> </param> <param name="output_type" type="select" force_select="true" label="Select format for output"> <option value="v" selected="true">Uncompressed VCF</option> <option value="z">Compressed VCF</option> <option value="u" selected="true">Uncompressed BCF</option> <option value="b">Compressed BCF</option> </param> </inputs> <outputs> <expand macro="vcf_output"/> <data name="output_gender_estimate" format="txt" label="${tool.name} (gender estimate) on ${on_string}"> <filter>output_gender_estimate_cond['output_gender_estimate'] == "yes"</filter> </data> </outputs> <tests> <test> <param name="reference_genome_source" value="history"/> <param name="history_item" value="sub50_adig_genome.fasta" ftype="fasta"/> <param name="annot" value="axiom_acropsnp_coral_annotation.r1.csv" ftype="csv"/> <param name="summary" value="sub50_axiomgt1.summary.txt" ftype="txt"/> <param name="snp_posteriors" value="sub50_axiomgt1.snp_posteriors.txt" ftype="txt"/> <param name="report" value="axiomgt1.report.txt" ftype="txt"/> <param name="confidences" value="sub50_axiomgt1.confidences.txt" ftype="txt"/> <param name="calls" value="sub50_axiomgt1.calls.txt" ftype="txt"/> <output name="output" value="output.vcf" ftype="vcf"/> </test> </tests> <help> This tool converts Affymetrix genotype calls and intensity files to VCF format. ----- **Required options** * **Probeset annotation file** - probeset annotation file produced by the sequencing run. * **Apt-probeset genotype summary file** - apt-probeset genotype summary file produced by the sequencing run. * **Apt-probeset genotype snp-posteriors file** - apt-probeset genotype snp-posteriors file produced by the sequencing run. * **Apt-probeset genotype report file** - apt-probeset genotype report file produced by the sequencing run. * **Apt-probeset genotype confidences file** - apt-probeset genotype confidences file produced by the sequencing run. * **Apt-probeset genotype calls file** - apt-probeset genotype calls file produced by the sequencing run. * **Choose the source for the reference genome** - select a reference genome from your history or one installed into your local Galaxy environment by a data manager tool. **Other options** * **Output apt-probeset-genotype gender estimate** - output apt-probeset-genotype gender estimate into an additional dataset. * **Append version and command line to the header** - append version and command line to the header of the output VCF dataset. * **Select format for output** - select one of uncompressed/compressed VCF/BCF. </help> <citations> <expand macro="citation1"/> <citation type="bibtex"> @misc{None, journal = {None}, author = {Genovese, Giulio}, title = {None}, year = {None}, url = {https://github.com/freeseek/gtc2vcf},} </citation> </citations> </tool>